Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,634 C→T 100% E38K (GAG→AAG)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,6340CT99.0% 3038.9 / 6.5 819E38K (GAG→AAG) yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (1/7);  new base T (370/440);  total (372/447)
Fisher's exact test for biased strand distribution p-value = 7.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 822 total aligned reads displayed.

AACTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAG  >  NC_000913/4633487‑4633780
                                                                                                                                                   |                                                                                                                                                  
 aCTGACAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGATGTAATCGGCGGGCATTGGCTACCCGTTTTCGTGCGCCAGCGCGCGTTTCCTCAGTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGAc                                                                                                                                                 <  1:8419066/150‑1 (MQ=255)
 aCTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGAc                                                                                                                                                 <  1:9254623/150‑1 (MQ=255)
 aCTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGAc                                                                                                                                                 <  2:5115874/150‑1 (MQ=255)
   tGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACgg                                                                                                                                               >  2:2587586/1‑150 (MQ=255)
   tGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACgg                                                                                                                                               <  2:5555007/150‑1 (MQ=255)
      aaGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCaa                                                                                                                                            <  2:5748382/150‑1 (MQ=255)
          gCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGa                                                                                                                                        <  1:9084664/150‑1 (MQ=255)
           cTGTCACCCTCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAt                                                                                                                                       <  1:695220/150‑1 (MQ=255)
           cTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAt                                                                                                                                       >  1:1475726/1‑150 (MQ=255)
           cTGTCACCATCGATGCCAGCGTCAATCGCCACCCAAAAATCATCCTCTGGAAGTAAACGGCGGGAATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAt                                                                                                                                       <  1:3634376/150‑1 (MQ=255)
            tGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATg                                                                                                                                      >  1:9367228/1‑150 (MQ=255)
               cACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCaa                                                                                                                                   <  1:1820584/150‑1 (MQ=255)
                  cATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAatat                                                                                                                                >  2:9086047/1‑150 (MQ=255)
                   aTCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATg                                                                                                                               >  1:827361/1‑150 (MQ=255)
                   aTCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATg                                                                                                                               >  2:9461175/1‑150 (MQ=255)
                     cGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGc                                                                                                                             >  1:8946465/1‑150 (MQ=255)
                     cGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGc                                                                                                                             <  2:1091379/150‑1 (MQ=255)
                      gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                            >  2:10789769/1‑150 (MQ=255)
                         gCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGa                                                                                                                         <  2:3514110/150‑1 (MQ=255)
                          ccAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTCCACCGCTCTTGACGGCACCGGATGCAAGATGGCAGGAt                                                                                                                        >  2:715470/1‑150 (MQ=255)
                          ccAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGAt                                                                                                                        >  2:9704581/1‑150 (MQ=255)
                           cAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCCCAGGGCTGTTCCGGTCCACCGCTCGTGACGGCCACGGATGCACTATGGCAGGATc                                                                                                                       >  2:465794/1‑150 (MQ=255)
                            aGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATcc                                                                                                                      >  2:1697999/1‑150 (MQ=255)
                            aGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATcc                                                                                                                      >  2:8977054/1‑150 (MQ=255)
                               ttCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTc                                                                                                                   >  2:10544183/1‑150 (MQ=255)
                                 cAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGc                                                                                                                 <  1:10155472/150‑1 (MQ=255)
                                   aTCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCg                                                                                                               >  2:4381903/1‑150 (MQ=255)
                                    tCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGa                                                                                                              >  1:7387923/1‑150 (MQ=255)
                                     cGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGACAACGGATGCAATATGGCAGGATCCTTCGCCGaa                                                                                                             <  1:2405610/150‑1 (MQ=255)
                                      gCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAg                                                                                                            <  1:1668371/150‑1 (MQ=255)
                                       ccACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGa                                                                                                           >  1:5713439/1‑150 (MQ=255)
                                       ccACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGa                                                                                                           >  2:1977990/1‑150 (MQ=255)
                                         aCCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtc                                                                                                         >  1:5049678/1‑150 (MQ=255)
                                         aCCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtc                                                                                                         >  2:8287806/1‑150 (MQ=255)
                                           ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                       >  1:10224083/1‑150 (MQ=255)
                                           ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                       >  2:4767677/1‑150 (MQ=255)
                                            cAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCg                                                                                                      >  1:6139961/1‑150 (MQ=255)
                                            cAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCg                                                                                                      <  1:2230925/150‑1 (MQ=255)
                                             aaaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGt                                                                                                     >  2:1459921/1‑150 (MQ=255)
                                             aaaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGt                                                                                                     >  2:7786056/1‑150 (MQ=255)
                                              aaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTg                                                                                                    >  2:5396414/1‑150 (MQ=255)
                                                  tCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAAt                                                                                                >  2:4912033/1‑150 (MQ=255)
                                                    aGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGc                                                                                              <  2:8751072/150‑1 (MQ=255)
                                                      ccTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCt                                                                                            <  1:9843369/150‑1 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          >  2:343521/1‑150 (MQ=255)
                                                        tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                          <  1:2547479/150‑1 (MQ=255)
                                                         ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCa                                                                                         <  1:2831390/150‑1 (MQ=255)
                                                         ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCa                                                                                         <  1:3933301/150‑1 (MQ=255)
                                                             aaGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAAt                                                                                     <  1:10003373/150‑1 (MQ=255)
                                                             aaGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAAt                                                                                     <  2:10161423/150‑1 (MQ=255)
                                                              aGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATg                                                                                    <  1:8099786/150‑1 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  >  2:3699321/1‑150 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  >  2:5248280/1‑150 (MQ=255)
                                                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                  <  1:6619890/150‑1 (MQ=255)
                                                                   aCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTg                                                                               >  2:2225355/1‑150 (MQ=255)
                                                                       cggGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAAtt                                                                           >  2:10732214/1‑150 (MQ=255)
                                                                        gggCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAAttt                                                                          >  2:699218/1‑150 (MQ=255)
                                                                         ggCTTTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAATGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCCCGTGAAATGCCTGCAGAATGGCCTGAAtttt                                                                         <  2:6004813/150‑1 (MQ=255)
                                                                          gCATTGGCTACCCGATTTCGTGCGCTAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTa                                                                        >  2:3049874/1‑150 (MQ=255)
                                                                               ggCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCggg                                                                   <  1:3383720/150‑1 (MQ=255)
                                                                                gCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                  >  2:10377737/1‑150 (MQ=255)
                                                                                gCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                  >  1:11115558/1‑150 (MQ=255)
                                                                                 cTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCACCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGAt                                                                 <  1:4061126/150‑1 (MQ=255)
                                                                                    cccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                              >  2:386896/1‑150 (MQ=255)
                                                                                    cccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                              >  2:9258152/1‑150 (MQ=255)
                                                                                    cccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                              >  1:5423819/1‑150 (MQ=255)
                                                                                     ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtggt                                                             <  1:10932304/150‑1 (MQ=255)
                                                                                        aTTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTggtggt                                                          >  2:2792462/1‑150 (MQ=255)
                                                                                            cGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGca                                                      <  2:2422583/150‑1 (MQ=255)
                                                                                             gTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcac                                                     >  2:5920094/1‑150 (MQ=255)
                                                                                                cgcCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                  >  2:8072438/1‑150 (MQ=255)
                                                                                                cgcCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                  <  2:1211243/150‑1 (MQ=255)
                                                                                                 gcCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCACTATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGGGGTCGCACAGAc                                                 >  2:6226897/1‑150 (MQ=255)
                                                                                                       gcgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGACAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTg                                           >  1:5279815/1‑150 (MQ=255)
                                                                                                              tCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa                                    <  1:2964679/150‑1 (MQ=255)
                                                                                                              tCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa                                    >  2:11106771/1‑150 (MQ=255)
                                                                                                               ccTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATaa                                   >  1:7731423/1‑150 (MQ=255)
                                                                                                                cTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                  >  1:9563205/1‑150 (MQ=255)
                                                                                                                 tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATc                                 >  1:1235224/1‑150 (MQ=255)
                                                                                                                   aCTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAg                               >  1:8636933/1‑150 (MQ=255)
                                                                                                                   aCTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAg                               >  2:1342436/1‑150 (MQ=255)
                                                                                                                   aCTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCAGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAg                               >  1:3173542/1‑150 (MQ=255)
                                                                                                                     tGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCa                             <  1:1977990/150‑1 (MQ=255)
                                                                                                                        cAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTa                          <  2:3335/150‑1 (MQ=255)
                                                                                                                        cAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTa                          <  1:4811947/150‑1 (MQ=255)
                                                                                                                        cAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTa                          >  2:6417293/1‑150 (MQ=255)
                                                                                                                        cAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTa                          >  2:402760/1‑150 (MQ=255)
                                                                                                                           aGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTc                       <  1:10544183/150‑1 (MQ=255)
                                                                                                                           aGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTc                       >  1:6002640/1‑150 (MQ=255)
                                                                                                                               cTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGaa                   <  2:2158940/150‑1 (MQ=255)
                                                                                                                                 gTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGaaaa                 <  2:8669747/150‑1 (MQ=255)
                                                                                                                                  ttCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                >  2:7356912/1‑150 (MQ=255)
                                                                                                                                  ttCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                >  1:4367864/1‑150 (MQ=255)
                                                                                                                                    ccGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTa              >  1:7889320/1‑150 (MQ=255)
                                                                                                                                    ccGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTa              >  1:4613560/1‑150 (MQ=255)
                                                                                                                                    ccGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTa              >  2:5634134/1‑150 (MQ=255)
                                                                                                                                    ccGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTa              >  1:6386162/1‑150 (MQ=255)
                                                                                                                                      ggTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAAc            <  2:6653122/150‑1 (MQ=255)
                                                                                                                                              gCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagc    <  1:6806223/150‑1 (MQ=255)
                                                                                                                                                tcttGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagcag  <  1:728338/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAG  >  NC_000913/4633487‑4633780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: