Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,873 C→G 7.2% intergenic (+84/‑260) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8730CG 7.2% 2045.4 / 24.8 624intergenic (+84/‑260)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (275/290);  new base G (48/2);  total (332/292)
Fisher's exact test for biased strand distribution p-value = 2.40e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 636 total aligned reads displayed.

TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCT  >  NC_000913/454724‑455022
                                                                                                                                                     |                                                                                                                                                       
  ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                                                                                       >  1:450852/1‑150 (MQ=255)
  ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                                                                                       >  2:5034919/1‑150 (MQ=255)
  ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTg                                                                                                                                                       >  1:9811580/1‑150 (MQ=255)
  ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTAGGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTGTCTg                                                                                                                                                       >  2:3875378/1‑150 (MQ=255)
   gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTgg                                                                                                                                                      >  1:9491111/1‑150 (MQ=255)
        gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                                                 <  2:1434242/150‑1 (MQ=255)
        gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                                                 >  1:1146288/1‑150 (MQ=255)
        gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGAtttt                                                                                                                                                 >  1:10116566/1‑150 (MQ=255)
         ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                                                                >  2:7000948/1‑150 (MQ=255)
         ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTAAGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                                                                >  2:8190530/1‑150 (MQ=255)
           gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTtcg                                                                                                                                              >  2:3969338/1‑150 (MQ=255)
           gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcg                                                                                                                                              <  1:157661/150‑1 (MQ=255)
             aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGAGCt                                                                                                                                            >  1:4220796/1‑150 (MQ=255)
              ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                                           >  2:668768/1‑150 (MQ=255)
               gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCttt                                                                                                                                          >  1:7807689/1‑150 (MQ=255)
               gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                                                                          <  1:712629/150‑1 (MQ=255)
               gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                                                                          <  1:10546905/150‑1 (MQ=255)
                 cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAATCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTATAGTTTTTATGAAAATCGGGGTGCGGGCCGTTTTGTTTTGTGTGGTTTTTGCGGttttt                                                                                                                                        >  2:8675292/1‑150 (MQ=255)
                   ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGc                                                                                                                                      <  1:7491729/150‑1 (MQ=255)
                   ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGCAAGCAACGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTGTCTGGATTTTTCGCATTTTGc                                                                                                                                      >  2:10619901/1‑150 (MQ=255)
                    cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGTGTGGATTTTTCGGTTTTTggc                                                                                                                                     >  1:4712508/1‑148 (MQ=255)
                       cTTTGCCTCTCCTCCCGGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                                                  <  1:5142117/150‑1 (MQ=255)
                        tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGc                                                                                                                                 <  2:9264577/150‑1 (MQ=255)
                        tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGc                                                                                                                                 <  1:1390626/150‑1 (MQ=255)
                         ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCa                                                                                                                                <  2:2602981/150‑1 (MQ=255)
                            ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGGTTTTTGGCTTTTTGACAAGCCtta                                                                                                                             >  1:10245640/1‑149 (MQ=255)
                            ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                                             >  2:3719814/1‑150 (MQ=255)
                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                            <  2:7482372/150‑1 (MQ=255)
                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                            >  1:3685467/1‑150 (MQ=255)
                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGGTTTGTCTGGAGTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                            >  2:4066970/1‑150 (MQ=255)
                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAAATGTCGGCGCTTTTCCAGCATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTGTTGGCTGGCTTTTGCGGTTTTTGCCCCGCATTcg                                                                                                                            >  1:10771799/1‑149 (MQ=255)
                              tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGGTTTTGCGCGTTTTGCCCAGCAATCAg                                                                                                                           >  2:2030584/1‑150 (MQ=255)
                                 cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                                                                        <  2:1739469/150‑1 (MQ=255)
                                       tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGGTTTTGCGCTTTTTGCCCAGCATTAAGACG‑AAAAt‑t                                                                                                                >  1:2370131/1‑150 (MQ=255)
                                         tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGc                                                                                                              >  2:6076686/1‑150 (MQ=255)
                                            tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCg                                                                                                           <  1:5471424/150‑1 (MQ=255)
                                            tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCg                                                                                                           <  2:10457406/150‑1 (MQ=255)
                                              gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCggg                                                                                                         >  2:599799/1‑150 (MQ=255)
                                               aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAGTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGa                                                                                                        >  1:3510027/1‑150 (MQ=255)
                                                    gAAGCATCGCGTAAAAATGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATtgt                                                                                                   <  1:4155052/150‑1 (MQ=255)
                                                    gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGGATtgt                                                                                                   >  2:5508136/1‑150 (MQ=255)
                                                     aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGTTTTTGGGCTTTTTGGCCAGCATTAATTCG‑AAAAT‑TTCCCGGGTATtttg                                                                                                  >  2:1138594/1‑150 (MQ=255)
                                                      aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGa                                                                                                 <  1:10941077/150‑1 (MQ=255)
                                                        cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGaaa                                                                                               <  2:11007931/150‑1 (MQ=255)
                                                            gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAAt                                                                                           <  1:1864069/150‑1 (MQ=255)
                                                             cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATa                                                                                          <  1:9963269/150‑1 (MQ=255)
                                                             cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTAGGGTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATa                                                                                          <  2:1266196/150‑1 (MQ=255)
                                                               tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATAcg                                                                                        <  2:3409595/150‑1 (MQ=255)
                                                               tAAAAACGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGTTTGTTTTTTCTTGATTTTTCGCTTTTTGCCCACCATTCAGACG‑AAAAT‑TTCCCGGGGATTGTGAAAAAATaag                                                                                        >  1:5810333/1‑148 (MQ=255)
                                                                aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATAcgc                                                                                       <  1:8393148/150‑1 (MQ=255)
                                                                    aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTTGATTTTTCGCTTTTTGCCCAGCATTCAGACG‑AAAAA‑TTCCCCGGAATTGTGTAAAAATAAGCGACa                                                                                   >  1:8194040/1‑150 (MQ=255)
                                                                      gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTCTTGGTTTTGGGGTTTTTTGCCACCAATAAGACG‑GAAAA‑TGGCCGGGGATTGTGTAAAAATAAGCGACAgc                                                                                 >  1:3000257/1‑150 (MQ=255)
                                                                        aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                               <  2:5412599/150‑1 (MQ=255)
                                                                         tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                                                              <  2:6838377/150‑1 (MQ=255)
                                                                          ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGTTTTTTGGCTTTTTTGCCAGCATTAAGAAG‑AAAAA‑TTCCCCGGCATTTTGTAAAAATAAGCCACAccgcgc                                                                             >  1:9595346/1‑150 (MQ=255)
                                                                          ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTTGGGTTTTTGGGCCGCAATAAGAAG‑AAAAA‑TTACCGGGAATTGTTAAAAAAAAAGCGACAgcgcgc                                                                             >  1:9402370/1‑150 (MQ=255)
                                                                            gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                                                           <  2:9151819/150‑1 (MQ=255)
                                                                              aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATAGTGAAAAAATACGCGACAGCGCGCAATa                                                                         >  2:6243729/1‑150 (MQ=255)
                                                                              aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTATGGATTTTTCGCTTTTTTACCAGCAAAAAGAAGAAAAAT‑TGCCGGGGAATT‑TGAAAAAAAAAACGACAgcgcgccaaa                                                                         >  1:8906623/1‑146 (MQ=255)
                                                                               aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                        >  2:8960947/1‑150 (MQ=255)
                                                                                   tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTTGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAACGt                                                                    >  2:2251335/1‑150 (MQ=255)
                                                                                   tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                                                                    >  2:6531943/1‑150 (MQ=255)
                                                                                    cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                                                                   <  2:2124718/150‑1 (MQ=255)
                                                                                     ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCAATCAGACG‑AAAAA‑TTCCCCGGAAATGTGAAAAAAAAAGCCACAGCGCGCAATAAACGTtc                                                                  >  2:1921782/1‑150 (MQ=255)
                                                                                     ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCCAAAACCGTtc                                                                  >  2:10523734/1‑150 (MQ=255)
                                                                                     ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                                                                  >  2:7905872/1‑150 (MQ=255)
                                                                                      gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGAGGATTTTTCGGTTTTTTACCAGCATTCAGACG‑AAAAA‑ATACCGGGAAGTGTGAAAAAAAAAGCGAAAGCCCGCAATAACCGAtct                                                                 >  1:6125988/1‑150 (MQ=255)
                                                                                       ctcTTTTCCAGTTTTTTGATAAAGTTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCATTCAGACT‑AATAT‑TGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                <  1:2284153/148‑1 (MQ=255)
                                                                                       cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                <  2:10116566/150‑1 (MQ=255)
                                                                                         cTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTGTGTGGGTTTTGGGCTTTTTTGCCAGCAATAAAACG‑AAAATATCCCCGGGAATT‑TGAAAAAATAAGCGACACCGCCCAAAAAACCATCTCGa                                                              >  1:4932712/1‑150 (MQ=255)
                                                                                                  tATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                     <  1:6855527/150‑1 (MQ=255)
                                                                                                  tATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                     <  1:4469266/150‑1 (MQ=255)
                                                                                                    tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                   <  1:2740440/150‑1 (MQ=255)
                                                                                                     gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg                                                  <  2:4649318/150‑1 (MQ=255)
                                                                                                          tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                             >  2:8355880/1‑150 (MQ=255)
                                                                                                           aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                            <  2:7691174/150‑1 (MQ=255)
                                                                                                            aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGACTTGTGCAAAAATAAGCGACAGCGGGCACTAACCGTTCTCGACTCATAAAAGTGATGCCGc                                           >  2:2993050/1‑150 (MQ=255)
                                                                                                                  ttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                                     <  2:9644433/150‑1 (MQ=255)
                                                                                                                   aaaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCGGACG‑AAAAT‑TGCCCGGGCATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTCTAATg                                    >  2:515286/6‑150 (MQ=255)
                                                                                                                     tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                  <  1:6653354/150‑1 (MQ=255)
                                                                                                                      acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                 >  2:2788830/3‑150 (MQ=255)
                                                                                                                       aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                <  2:4905683/150‑1 (MQ=255)
                                                                                                                       aaaaaCGGCCTGCGGGCCGATTTGTATTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                <  1:5296818/150‑1 (MQ=255)
                                                                                                                        aaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                               <  1:7000948/150‑1 (MQ=255)
                                                                                                                         aaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGt                              >  2:5621616/1‑150 (MQ=255)
                                                                                                                               ccTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                        <  2:3174102/150‑1 (MQ=255)
                                                                                                                               ccTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                        <  2:9491111/150‑1 (MQ=255)
                                                                                                                               ccTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                        <  1:7354963/150‑1 (MQ=255)
                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                       <  2:2470737/150‑1 (MQ=255)
                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                       <  2:7318607/150‑1 (MQ=255)
                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                       <  1:6488612/150‑1 (MQ=255)
                                                                                                                                 tGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTg                      <  1:313540/150‑1 (MQ=255)
                                                                                                                                  gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa                     <  1:1994492/150‑1 (MQ=255)
                                                                                                                                  gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa                     <  1:3875501/150‑1 (MQ=255)
                                                                                                                                  gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa                     <  1:1803876/150‑1 (MQ=255)
                                                                                                                                          ttttgttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc             >  2:1571748/1‑150 (MQ=255)
                                                                                                                                           tttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg            <  2:10662187/150‑1 (MQ=255)
                                                                                                                                           tttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg            <  1:8687738/150‑1 (MQ=255)
                                                                                                                                            ttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg           <  2:450852/150‑1 (MQ=255)
                                                                                                                                                    tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc   >  2:4265334/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCT  >  NC_000913/454724‑455022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑