Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,015 T→A 6.5% intergenic (+258/‑319) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0150TA 6.5% 1885.8 / 15.4 606intergenic (+258/‑319)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base T (234/309);  new base A (58/2);  total (295/311)
Fisher's exact test for biased strand distribution p-value = 1.28e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 622 total aligned reads displayed.

ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164
                                                                                                                                                     |                                                                                                                                                      
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAt                                                                                                                                                        <  2:5865832/150‑1 (MQ=255)
 tGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAtt                                                                                                                                                       >  1:10165040/1‑150 (MQ=255)
    tctcTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTAt                                                                                                                                                    <  1:5556929/150‑1 (MQ=255)
     ctctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                   <  1:6046988/150‑1 (MQ=255)
     ctctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                   <  1:1347878/150‑1 (MQ=255)
     ctctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                   <  2:3420353/150‑1 (MQ=255)
         gCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATATGGCGTTCACGCCGCATCCGGCAATAAACAGCAAAAAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCata                                                                                                                                               <  1:4261/150‑1 (MQ=255)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataa                                                                                                                                              <  1:3529987/150‑1 (MQ=255)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCaaaa                                                                                                                                              >  1:1736845/1‑150 (MQ=255)
              atatTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaata                                                                                                                                          <  1:10954907/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                        <  2:4627432/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                        <  2:6589331/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGAATCCGTCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                        <  1:5220603/150‑1 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAaaataaat                                                                                                                                       <  1:1498193/150‑1 (MQ=255)
                     gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaa                                                                                                                                   <  1:6828549/150‑1 (MQ=255)
                      tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaa                                                                                                                                  <  1:1994774/150‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGCAAGAAACAGCTAACAATCCACACCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCGGCTTCGCAATTTATCCATTATATAAATTTAAAATaa                                                                                                                              <  2:4017488/150‑1 (MQ=38)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                              <  2:1657430/150‑1 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTAGCGAATTAATTCCGCTTCGCAAATTATCCAAAAAATAAAATTAAAATAACaaaa                                                                                                                         >  1:2656050/1‑150 (MQ=39)
                                tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAACCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAAc                                                                                                                        <  1:6400943/150‑1 (MQ=255)
                                tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAATAAACAAAAc                                                                                                                        >  2:7151021/1‑150 (MQ=255)
                                      ggCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAtt                                                                                                                  >  2:8700692/1‑150 (MQ=255)
                                       gCCGGATGCGGCGTGAACGCCTTATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAATCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATta                                                                                                                 >  2:2400384/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTCTTCGCGAATTAATTACGCGTCGCAATTTATCCATAAAAAAAATTTAAAATAACAAAACAAAATta                                                                                                                 >  1:10026540/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCAAAAAATAAATTTAAAAAAACAAAAAATAATta                                                                                                                 >  1:9962469/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTAGTCGCCAAATAATTCCCCGTCGCAAGTTAAACAAAAAATAAAATTAAAATAAAAAAAaataaata                                                                                                                 >  1:10569354/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTCGCCAATTAAATACGCTTAGCAAATTATACAAAAAAAAAAATTAAAATAAAAAAAAAAAATta                                                                                                                 >  1:5318069/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTCGTCGCGAAAAAATTCCGCGTCGCAATTTATCCCTAAAATAAATTTAAAAAAACAAAACAAAAAta                                                                                                                 >  2:6788888/1‑150 (MQ=255)
                                         cGGATGCGGCGTTAACGCCTTATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaa                                                                                                               <  2:985328/150‑1 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAGCAAAACATAATtaaa                                                                                                               <  2:8399355/150‑1 (MQ=255)
                                           gATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGGATTTATTCCGCGTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAAAAAAaataaata                                                                                                             >  1:4456476/1‑150 (MQ=255)
                                           gATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCCATTTATACAAAAAATAAATTTAAAATAAAAAAACATAATtaaata                                                                                                             >  2:1216111/1‑150 (MQ=255)
                                           gATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCAGCTTCGCAATTTATACATAAAATAAATTTAAAATAAAAAAAaataaataaata                                                                                                             >  1:7211925/1‑150 (MQ=255)
                                              atggCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                          <  2:1106641/148‑1 (MQ=255)
                                                 gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATACATAAAATAAATTTAAAATAACAAAAAATAATTAAATAAAATGt                                                                                                       >  2:7907205/1‑150 (MQ=255)
                                                 gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTGTCGGCGTTTCTTCCCGAAATAATTACCCGGCCCAAAATAACCAAAAAAAAAAGTTAAAAAAACAAAAAATAATTAAATAAAAAGt                                                                                                       >  1:7970982/1‑150 (MQ=255)
                                                 gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGAGTTCAGCGGCGTTTTTTGTTGTTTTAATAGCGAAATAAATAAGAGTAGCAATTTATAAATAAAAAAAAATTAAAAAAAAAAAAAATAAATAAATAAAAttt                                                                                                       >  1:8809937/1‑148 (MQ=255)
                                                    ttCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGGTTTTCTGCGCGAATTAATTCCCCTTCGCACTTTATGCATAAAAAAAATTTAAAAAAACAAAAAAAAATTTAATAAAAAGTaca                                                                                                    >  1:8670714/1‑148 (MQ=255)
                                                     tCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCGTTTCTTCGCGAATTAATTCCGCGTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAAcc                                                                                                   >  2:4178331/1‑150 (MQ=255)
                                                      cACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATTTAAACg                                                                                                  >  2:10668079/1‑150 (MQ=255)
                                                       aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGc                                                                                                 <  2:2212938/150‑1 (MQ=255)
                                                       aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGc                                                                                                 <  2:8941202/150‑1 (MQ=255)
                                                       aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAAAAAACATAATTAAATAAAATGTAACCGc                                                                                                 >  2:688522/1‑150 (MQ=255)
                                                          ccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCGTCGCGAATTAATTCCGCTTCGCAAATTATACATAAAATAAAATTAAAATAACAAAACATAAATAAATAAAATGTAACCGCttt                                                                                              >  2:3056854/1‑150 (MQ=255)
                                                           cgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTGTTAGCGAATTAATTACGCGTCGCAAATTATCACTAAAATAAATTTAAAATAAAAAAACAAAAATAAATAAAATGTAAACGcgatc                                                                                             >  1:10721639/1‑146 (MQ=255)
                                                           cgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTc                                                                                             <  2:8188706/150‑1 (MQ=255)
                                                             cATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTATGCTTTTGTTCGCGAATTAATTAAGCGTAGCCATTTATCCAAAAAATAAATTTAAAATAAACAAAAATAAATAAATATAATGTAAACGCgttgag                                                                                           >  1:9452941/1‑147 (MQ=255)
                                                              aTCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATc                                                                                          <  2:6359617/150‑1 (MQ=255)
                                                               tCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCt                                                                                         <  2:4929456/150‑1 (MQ=255)
                                                               tCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATAAATTCCGCTTAGCAATTTATACATAAAATAAATTTAAAATAAAAAAAAATAATAAAATAAAATTTAAACGCTTTAAtat                                                                                         >  2:1020654/1‑148 (MQ=255)
                                                                  ggCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGc                                                                                      <  2:4297388/150‑1 (MQ=255)
                                                                   gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                     <  1:3009245/150‑1 (MQ=255)
                                                                   gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                     <  1:5466653/150‑1 (MQ=255)
                                                                       gAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGaa                                                                                <  1:8128285/150‑1 (MQ=255)
                                                                       gAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGaa                                                                                <  1:9226480/150‑1 (MQ=255)
                                                                        aaaCCGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAt                                                                               <  1:5777265/150‑1 (MQ=255)
                                                                          aCAGCAAACAATCCAACACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAttt                                                                             <  2:5689142/150‑1 (MQ=255)
                                                                           cAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTc                                                                            <  1:7022669/150‑1 (MQ=255)
                                                                            aGCAAACAATCCACAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                           <  2:2768788/150‑1 (MQ=255)
                                                                            aGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                           <  1:8356338/150‑1 (MQ=255)
                                                                             gCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCAc                                                                          <  2:6191584/150‑1 (MQ=255)
                                                                              cAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACg                                                                         <  2:9115412/150‑1 (MQ=255)
                                                                                       cAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAAAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTTCTGGGATTTCACGCTTTTAttc                                                                >  2:1780001/1‑150 (MQ=255)
                                                                                        aaaaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTAACGCTTTTAttct                                                               >  2:7309780/1‑150 (MQ=255)
                                                                                        aaaaCGCCGCGTTCAGCGGCGTTTTTTCTGCGTTTCGTAGCGAATTAATTCCCCGGCGCAAATTATCCAAAAAAAAAAATTAAAAAAAAAAAACAAAAATAAAAAAAATGTAACCGCGTTCAAAGTGCTGGAATTTAACGCTTTTAttat                                                               >  2:4803349/1‑148 (MQ=255)
                                                                                         aaaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAATAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCGTTTAttctt                                                              >  2:4557413/1‑150 (MQ=255)
                                                                                          aaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttcttc                                                             <  2:5498426/150‑1 (MQ=255)
                                                                                             gccgcGTTCAGCGGCGTTTTTTCTGCTTTTATTAGCGAATTAATTCCGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTGTCAACTTGCTGGCATTTCACGCTTTTATTCTTCTGc                                                          >  2:615567/1‑150 (MQ=255)
                                                                                              ccgcGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCa                                                         <  2:7521414/150‑1 (MQ=255)
                                                                                                  gTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAATAAATAAAATGTAACCGCTTTCATAATGCTGGAATTTCAAGCTTTTATTCTTATGCAAGcc                                                     >  2:10799738/1‑150 (MQ=255)
                                                                                                  gTTCAGCGGCGTTTTTTCTGCTTTTCATCGCGAATTAATTCCCCGTCGCAAATTATCCCAAAAAAAAATTTAAAAAAACACAACATAAATAAAAAAAAATTAACCCCTTGCATCATGCTGGAAATTCACGCGGTTATTCTTCTGCAAGcc                                                     >  2:9333925/1‑150 (MQ=255)
                                                                                                             ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                          <  1:6910284/150‑1 (MQ=255)
                                                                                                             ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                          <  2:9483555/150‑1 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAATCCTTTCAACCGCAAACTTaa                                   >  1:6631280/1‑150 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   <  1:2807038/150‑1 (MQ=255)
                                                                                                                    tGCCTTTCTTCGCGAAGTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAACTAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   <  1:2193037/150‑1 (MQ=255)
                                                                                                                     gCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAg                                  >  1:9725585/1‑150 (MQ=255)
                                                                                                                      cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                 >  1:3850736/1‑150 (MQ=255)
                                                                                                                      cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                 >  2:7765842/1‑150 (MQ=255)
                                                                                                                       ttttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGcc                                >  2:9755919/1‑150 (MQ=255)
                                                                                                                       ttttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGcc                                >  1:6193586/1‑150 (MQ=255)
                                                                                                                         ttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                              <  1:3278091/150‑1 (MQ=255)
                                                                                                                          tcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTg                             <  1:10431982/150‑1 (MQ=255)
                                                                                                                          tcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTg                             <  1:2525534/150‑1 (MQ=255)
                                                                                                                          tcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTg                             <  2:3835357/150‑1 (MQ=255)
                                                                                                                           cttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGt                            <  2:3227996/150‑1 (MQ=255)
                                                                                                                            ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTa                           <  2:1984714/150‑1 (MQ=255)
                                                                                                                               gcgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACa                        >  1:3624116/1‑150 (MQ=255)
                                                                                                                                cgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaa                       <  1:4036664/150‑1 (MQ=255)
                                                                                                                                   attaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaaa                    <  1:4896835/150‑1 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   <  2:288887/150‑1 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   <  2:2609644/150‑1 (MQ=255)
                                                                                                                                       attCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatca                 <  2:5411997/150‑1 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  1:7480250/150‑1 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               >  1:9133262/1‑150 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca              >  2:882847/1‑150 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTCGTAACAAAAAtcatca              >  2:5157145/1‑150 (MQ=255)
                                                                                                                                           cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa            <  2:3699166/150‑1 (MQ=255)
                                                                                                                                           cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa            >  2:60154/1‑150 (MQ=255)
                                                                                                                                                  cAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgtg     >  2:1440325/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: