Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 843,205 T→G 7.0% W292G (TGG→GGG)  rlmF → 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913843,2050TG 7.0% 1526.1 / 6.7 548W292G (TGG→GGG) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (129/312);  new base G (56/2);  total (234/314)
Fisher's exact test for biased strand distribution p-value = 1.39e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 561 total aligned reads displayed.

GCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGG  >  NC_000913/843056‑843354
                                                                                                                                                     |                                                                                                                                                     
gCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCt                                                                                                                                                       >  1:10418401/1‑150 (MQ=255)
gCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCt                                                                                                                                                       <  1:2046256/150‑1 (MQ=255)
 cTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTg                                                                                                                                                      <  2:5807050/150‑1 (MQ=255)
 cTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTg                                                                                                                                                      <  2:5429393/150‑1 (MQ=255)
  ttCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCTgg                                                                                                                                                     >  2:160207/1‑150 (MQ=255)
   tCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGa                                                                                                                                                    >  2:7218527/1‑150 (MQ=255)
    cgcgAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGAc                                                                                                                                                   <  2:9341755/150‑1 (MQ=255)
        aaGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCttt                                                                                                                                               <  1:10138616/150‑1 (MQ=255)
        aaGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCttt                                                                                                                                               <  1:79682/150‑1 (MQ=255)
        aaGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCttt                                                                                                                                               <  2:4142019/150‑1 (MQ=255)
        aaGCAGGTGATGTGGTGTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGCGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCttt                                                                                                                                               <  2:86856/150‑1 (MQ=255)
         aGCAGGTGATGTGGATTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTa                                                                                                                                              <  2:898616/150‑1 (MQ=255)
          gCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTGTTTCCTGGACCGTTAt                                                                                                                                             >  1:1235433/1‑150 (MQ=255)
          gCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTAt                                                                                                                                             <  2:1699056/150‑1 (MQ=255)
          gCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTAt                                                                                                                                             <  1:4063164/150‑1 (MQ=255)
             ggTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGaa                                                                                                                                          <  2:2300730/150‑1 (MQ=255)
             ggTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGACAGGACCTTTATTaa                                                                                                                                          >  2:10834361/1‑150 (MQ=255)
                   gtgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCATGACCATTAAGTAagacga                                                                                                                                    >  2:10577208/1‑150 (MQ=255)
                    tgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTACAGGACCATTATGaaacaccag                                                                                                                                   >  1:4849564/1‑143 (MQ=255)
                    tgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCTGGACCTTTATGTACGACGag                                                                                                                                   >  1:7114081/1‑150 (MQ=255)
                    tgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCAGGACCTTTATTAAACACGag                                                                                                                                   >  1:134296/1‑150 (MQ=255)
                     ggTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGGTATTGACAGGGCCATTATTAAAGACGagc                                                                                                                                  >  1:2959568/1‑150 (MQ=255)
                      gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCGGGACCTTTATGTACGACGagca                                                                                                                                 >  1:5845018/1‑150 (MQ=255)
                      gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGACCATTATGAACGACGagca                                                                                                                                 >  1:5954399/1‑150 (MQ=255)
                        ttACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAACCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagcagc                                                                                                                               <  1:6283542/150‑1 (MQ=255)
                         tACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAgcg                                                                                                                              <  1:9782864/150‑1 (MQ=255)
                             tCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTACAGGACCGATATTAAAGACGAGCAGcgccgc                                                                                                                          >  1:5191563/1‑150 (MQ=255)
                              cACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTCCTGGTCCGTTATGAACGACCAGccgccccgcc                                                                                                                         >  1:96760/1‑150 (MQ=255)
                              cACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCGGGACCATTATTAAACACCACCAGCgccccc                                                                                                                         >  2:6589919/1‑150 (MQ=255)
                                cTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCTTTATTAAAGAAGAGCATCGCCGCCGt                                                                                                                       >  2:559129/1‑150 (MQ=255)
                                  ggTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGttt                                                                                                                     <  2:2085767/150‑1 (MQ=255)
                                   gTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGtttt                                                                                                                    <  1:2436055/150‑1 (MQ=255)
                                     aTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGt                                                                                                                  <  2:5777607/150‑1 (MQ=255)
                                        tcGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCTGGACCATTATGAAACACGAGCAGCCCCGCCGGTTTGTCaa                                                                                                               >  1:1744857/1‑150 (MQ=255)
                                        tcGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCaa                                                                                                               >  2:858163/1‑150 (MQ=255)
                                             gtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGGTTTTTCCTGGTCCATTATGGAACACCAGCAGCGCCGCCGGTTTTTCAATAGcc                                                                                                          >  2:4001596/1‑150 (MQ=255)
                                              tgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGTCCGTTATTAACGAACAGCAGCGCCGCCCTTTTTTCAAACGCCa                                                                                                         >  1:11038066/1‑150 (MQ=255)
                                              tgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGACCTTTATTAACGAACCGCAGCGCCGCCGGTTTGTCAATAGccc                                                                                                         >  2:3084018/1‑149 (MQ=255)
                                                aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCTTTATTAACGACGAGCAGCGCCGCCCTTTTGTCAATACCCAGc                                                                                                       >  2:10926141/1‑150 (MQ=255)
                                                aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTGCCTGGACCATTAAGAACGACGAGCAGCGCCCCCCTTTTGTCAAtacccacc                                                                                                       >  2:7721286/1‑143 (MQ=255)
                                                 aaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCg                                                                                                      <  2:95048/150‑1 (MQ=255)
                                                          gccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTTTTTACAGGACCATTATTTAAGACGAGCCGCGCCGCCCTTTTGTGAAACGCCAGCGTTAAAGCGt                                                                                             >  1:3399829/1‑150 (MQ=255)
                                                            cgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTg                                                                                           <  1:3780202/150‑1 (MQ=255)
                                                             gTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGACCGTTATTAAAGACGAGCAGCGCCGCCCTTTTGTCAATCGCCCGCCTGAAAGCGTTgg                                                                                          >  1:9986749/1‑150 (MQ=255)
                                                             gTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTgg                                                                                          <  1:8250313/150‑1 (MQ=255)
                                                                gTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTc                                                                                       <  1:8622052/150‑1 (MQ=255)
                                                                 tATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTcc                                                                                      <  1:5297743/150‑1 (MQ=255)
                                                                 tATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTcc                                                                                      <  1:6607735/150‑1 (MQ=255)
                                                                  aTCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGGTTTTGCCGGGAACTTTATGAAAGAAGCGCAGCGCCGCCGGTTTTGCAATAGCCAGCCGTAAATCGTTGGTTacc                                                                                     >  1:10686705/1‑150 (MQ=255)
                                                                   tCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCAGGACCATTAAGAAAGACGAGCAGCGCCGCCCTTTTTTAAATCGCCAGCCTTAAATCGGTGGTTcccc                                                                                    >  2:1154882/1‑150 (MQ=255)
                                                                      tGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCTTTATGAACCACCAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGGTAAAGCGGTGGTTCCCCcgg                                                                                 >  2:6032146/1‑150 (MQ=255)
                                                                        cccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcggcg                                                                               <  2:3505027/150‑1 (MQ=255)
                                                                           tGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCTTTATTAACGACCAGCCGCGCCCCCCGTTTTTCAAAAGCCACCCGAAAAGCGGTGGGTCCCCccgccgga                                                                            >  2:10397441/1‑146 (MQ=255)
                                                                             acggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAAc                                                                          <  1:10506546/150‑1 (MQ=255)
                                                                              cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACg                                                                         <  1:160207/150‑1 (MQ=255)
                                                                              cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACg                                                                         <  1:761988/150‑1 (MQ=255)
                                                                               ggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGGCCGTTAAGAACGCCCAGCCCCCCCGCCCTTTTGTGAAACCCCACCGGTAAACACTTGGTTGCCCCCGCGGGAAccg                                                                        >  1:6841357/1‑148 (MQ=255)
                                                                                gtcgTGGGCGCGGTGTAGGTTGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGTCTAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTTAAGCGTTGGTTCCCCCGGCGGTAACGGt                                                                       <  2:10633845/148‑1 (MQ=255)
                                                                                 acgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTACAGGACAATTATGAAACACGAGCAGCGCCCCCCTTTTTTAAAAAGCCACCGTTAAATCGTTTGTTACCCCCGCGGTAACGGtt                                                                      >  2:4419473/1‑150 (MQ=255)
                                                                                 acgGGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGtt                                                                      <  2:6188485/150‑1 (MQ=255)
                                                                                   gTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCa                                                                    <  2:2529891/150‑1 (MQ=255)
                                                                                    ggggCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAg                                                                   <  2:4949695/149‑1 (MQ=255)
                                                                                       gcgcGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGAACTATAAGAAAGACGAGCAGCGCCCCCGATTTGTCAAACGCCACCGTTAAAGAGTTGGTTACCCCCGCGGTAACCGTTCAGCCg                                                                >  2:7901771/1‑150 (MQ=255)
                                                                                           ggTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGt                                                            <  2:10675299/150‑1 (MQ=255)
                                                                                           ggTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCGTTATTAACCACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGGTAAATCGTTGGTTACCCCCGCGGTAACGGTTCAGCCGGGGt                                                            >  2:711650/1‑150 (MQ=255)
                                                                                            gTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCAGGGCCGTTATTAAACAAGAGCAGCCCCCCCCATTTTTTAAAAGACACCCTTAAAAACTTTGTTACCCCCGCGGGAAAGGTTAAGCCcggggc                                                           >  1:1512326/1‑148 (MQ=255)
                                                                                               aaGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTcgcg                                                        <  2:8268089/150‑1 (MQ=255)
                                                                                                  gtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGACCATTAATAACCACCAACACCCCCGCCCATTTTTCAAAACCCAGCCTTAAAACCTTTGTTACCCCCGCCGTAACCGTTCATCCCGGGTCcccccc                                                     >  2:3422657/1‑150 (MQ=255)
                                                                                                  gtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGACCCTTAAGAAACACCAGCAGCGCCCCCCTTTTGTCAAAAGCCCGCGGTAAAGCGTTGGTTACCCCCGCGGGAACGGGTCAGCCCGGGTCGCGccc                                                     >  2:1457729/1‑150 (MQ=255)
                                                                                                     gtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAAGCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGa                                                  <  1:4992265/150‑1 (MQ=255)
                                                                                                       tAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCGTTATGAACGCCGAGCAGCCCCGCCGGTTTGTCAATACCCAGCGTTAAAGCGTTGGTTCCCCCCGCGGGAACGGTTCAGCCGGGGTCGCGCCCGGAgc                                                >  2:4591268/1‑150 (MQ=255)
                                                                                                          aaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCGGGACCATTATTAAACACCAGCAGCGCCCCCCGTTTGTCAAACGCCACCGGTAAAACGGGGGTTGCCCCCGCGGGGACCGTGCAGCCGGGGGCGCGCCCCGCgcacg                                             >  2:3803257/1‑148 (MQ=255)
                                                                                                                    gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATTAACGACGAGCAGCGCCGCCGGTTTGTCAATAGCCAGCGTTAAAGCGGTGGTTCCCCCCGCGGTAACCGGTCAGCCGGGGTCGCGCCCCGAGCAGGCAGCACCAGa                                   >  2:2400212/1‑150 (MQ=255)
                                                                                                                    gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGa                                   <  2:4517667/150‑1 (MQ=255)
                                                                                                                    gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGa                                   <  2:9115897/150‑1 (MQ=255)
                                                                                                                      ccAGGGGCAAAAGCAGAGTCGCTTTAATGGCGGGGCCATTATGAACGACCAGCAGCGCCGCCCGTTTGGCCAGCCCCAGCGGTAAAGCGGTGGGTGCCCCCGCGGTAACGGGTCAGCCGGGGGCGCGCCCGGAGCAGGCAGCACCAGATa                                 >  2:389731/1‑150 (MQ=255)
                                                                                                                      ccAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGCACTTTATGAACGACCAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGGTAAAGCGTTGGTTCCCCCCGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATa                                 >  2:7400958/1‑150 (MQ=255)
                                                                                                                       cAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                                <  1:8676323/150‑1 (MQ=255)
                                                                                                                       cAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                                <  2:6737684/150‑1 (MQ=255)
                                                                                                                       cAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                                <  2:2903046/150‑1 (MQ=255)
                                                                                                                        aGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCCTTTTGTCAAAAGCCAGCCGTAAAGCGTTGGTTCCCCCCGCGGGAACGGGTCAGCCGGGGTCGCGCCCGGCGCAGGCAGCACCGGAGAAg                               >  2:9932936/1‑150 (MQ=255)
                                                                                                                          gggCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGCCGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTc                             <  2:6962515/150‑1 (MQ=255)
                                                                                                                           ggCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCt                            <  2:4728230/150‑1 (MQ=255)
                                                                                                                           ggCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCt                            <  1:2280813/150‑1 (MQ=255)
                                                                                                                             cAAAAGCAGAGTCGCTTTATTGCCGGGCCCCTTATGTACGAAGAGCAGCGCCCCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTTGTTCCCCCGGCGGTAACGGGTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGc                          >  2:2656949/1‑150 (MQ=255)
                                                                                                                             cAAAAGCAGAGTCGCGTTATTGCCTGGACCTTTATTAACGACGAGCAGCGCCCCCGTTTTGTCAATCGCCAGCGTTAAAGCGGTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGc                          >  2:8181133/1‑150 (MQ=255)
                                                                                                                                    agagTCGCTTTATTGCCTGGACCTTTATGAAACACGAGCAGCGCCGCCCTTTTGTAAATAGCCAGCGGTAAAGCGTTGGGTCCCCCCGCGGTAAAGGTTAAGCCGGGGTCGCGCCCCGAGTAGGCACCACCAGATAAGTCAGCACTGgcg                   >  2:10209371/1‑150 (MQ=255)
                                                                                                                                      agTCGCTTTATTGCCTGGACCTTTATTAACGACGAGCAGCGCCGCCGTTTTGTCAAACGCCAGCGTTAAAGCGTTGGTTCCCCCCGCGGTAAACGTTCAGCCCGGGTCGCGCCCGGAGCAGGCAGCACCAGATAAGTCTGCACAGgcgcg                 >  2:127836/1‑150 (MQ=255)
                                                                                                                                      agTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGgcgcg                 <  1:2660161/150‑1 (MQ=255)
                                                                                                                                       gTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGgcgcgc                <  1:10021195/150‑1 (MQ=255)
                                                                                                                                        tCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGcgcgcg               <  1:4221970/150‑1 (MQ=255)
                                                                                                                                           cTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAc            >  2:6397288/1‑150 (MQ=255)
                                                                                                                                             ttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgc          <  2:9806560/150‑1 (MQ=255)
                                                                                                                                              tATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTTATAAGTCTGCACTGGCGCGCGAAcgcc         <  1:7776486/150‑1 (MQ=255)
                                                                                                                                              tATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcc         <  2:5191563/150‑1 (MQ=255)
                                                                                                                                                ttGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcccg       <  2:3275122/150‑1 (MQ=255)
                                                                                                                                                 tGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcccgc      <  2:5896399/150‑1 (MQ=255)
                                                                                                                                                  gCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAg                                                 <  1:4588699/106‑1 (MQ=255)
                                                                                                                                                   ccTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCa    >  2:1919412/1‑150 (MQ=255)
                                                                                                                                                    cTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcggcg                                                                               <  1:9299158/74‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTCGCGAAGCAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGG  >  NC_000913/843056‑843354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: