Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,910,574 T→G 5.3% L100R (CTT→CGT)  yebQ → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,910,5740TG 5.3% 1688.2 / 4.6 535L100R (CTT→CGT) yebQputative transporter
Reads supporting (aligned to +/- strand):  ref base T (153/331);  new base G (43/2);  total (202/333)
Fisher's exact test for biased strand distribution p-value = 5.41e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 556 total aligned reads displayed.

AGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTATTGTTG  >  NC_000913/1910425‑1910723
                                                                                                                                                     |                                                                                                                                                     
  ccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCttt                                                                                                                                                     <  2:422268/150‑1 (MQ=255)
  ccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCttt                                                                                                                                                     <  1:1827676/150‑1 (MQ=255)
  ccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTAATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGTCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTATGTTGTCTTAACTGTTCTGCGCCCtgt                                                                                                                                                     >  1:7862771/1‑148 (MQ=255)
      ttCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGTGGTCTGGTCGTTTTTCTGTTGTCTTCACGGTTCTGCGCCCGTTCGGa                                                                                                                                                 >  2:8567369/1‑150 (MQ=255)
        ccATCTGTGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCGCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGAtt                                                                                                                                               <  2:10240809/150‑1 (MQ=255)
          aTCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCg                                                                                                                                             >  1:7437553/1‑150 (MQ=255)
          aTCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCg                                                                                                                                             <  2:3201363/150‑1 (MQ=255)
          aTCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCg                                                                                                                                             <  2:6150371/150‑1 (MQ=255)
          aTCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCAAGGTTCTGCGCCCGTTCTGATTCg                                                                                                                                             >  1:8886127/1‑150 (MQ=255)
              gggTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGc                                                                                                                                         <  1:1575081/150‑1 (MQ=255)
               ggTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCGTTCTGATTCGCTGCa                                                                                                                                        >  1:6904904/1‑150 (MQ=255)
               ggTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGGTGTCTTCACTGTTCTGCGCCCGGGCTGATTCGCTGCa                                                                                                                                        >  2:9160481/1‑150 (MQ=255)
                 tAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTCTGTTGTCTTCACGGTTCTGCGCCCTTTCTGATTCGCTGCaaa                                                                                                                                      >  2:3901471/1‑150 (MQ=255)
                    tGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGc                                                                                                                                   <  2:5925943/150‑1 (MQ=255)
                      aaCGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTc                                                                                                                                 >  2:2816228/1‑150 (MQ=255)
                              tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTTTTGTCTTCACAGTTCTGCGCCCGTTCTGATTCGCTGCAAAAGCTCACCCATgc                                                                                                                         >  1:3189111/1‑150 (MQ=255)
                              tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTgc                                                                                                                         >  2:7388952/1‑150 (MQ=255)
                              tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCGTCACTGTTCTGCGCCCGGTCGGATTCGCTGCAAATGCTCACCCATgc                                                                                                                         >  1:9735173/1‑150 (MQ=255)
                              tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTGTGTTGTGTTCACTGTTCTGCGCCCGGTCGGATTCGCGGCAAATGCTCACCCATgc                                                                                                                         >  1:3833048/1‑150 (MQ=255)
                                aaaTCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGTGTTGTCTTGACTGTTATGCGCCCGTTATGGTTAGCTGCAAAAGCTCACCCGTgcgc                                                                                                                       >  1:2251721/1‑150 (MQ=255)
                                aaaTCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTgcgc                                                                                                                       <  2:1697829/150‑1 (MQ=255)
                                  aTCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCgt                                                                                                                     <  2:1231528/150‑1 (MQ=255)
                                     gCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTTTTTTTTGTTGAATTTTGTGGGGCCTTTGTGATTGGCTGCAAATTATCAAACTTTCGCgtgta                                                                                                                  >  1:8342342/1‑149 (MQ=255)
                                     gCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTc                                                                                                                  >  2:7608970/1‑150 (MQ=255)
                                         ttGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATAc                                                                                                              >  2:8680886/1‑150 (MQ=255)
                                         ttGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTATGTTGTATTCACAGTTCTGCGCCCGTTCTGATTAGCTGCACAAGCTCACCCATGCGCGTGTCATaa                                                                                                              >  1:281841/1‑149 (MQ=255)
                                           gTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGTCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACaa                                                                                                            <  1:1911211/150‑1 (MQ=255)
                                           gTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACaa                                                                                                            <  2:717245/150‑1 (MQ=255)
                                             cATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAgg                                                                                                          <  1:8210834/150‑1 (MQ=255)
                                             cATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAgg                                                                                                          <  2:5263852/150‑1 (MQ=255)
                                              aTCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGTTTTGTGTTAAATGTTGTGAGCCCGTTCTGATTCGCTGCAAAATATCACCCATGCGCGTGTCATACAAGGt                                                                                                         >  1:5758465/1‑150 (MQ=255)
                                                ctcCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGttt                                                                                                       <  2:5049211/150‑1 (MQ=255)
                                                 tcCCTGCTCGCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACAGTTCTGCGCCCATTCTGATTCGCTGCAAATGCTCACCCATGCGCGTGTCATACAAGGTTTc                                                                                                      >  2:9757693/1‑150 (MQ=255)
                                                      gCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTcggcgg                                                                                                 <  2:8144560/150‑1 (MQ=255)
                                                        tctcGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTg                                                                                               <  2:10666592/150‑1 (MQ=255)
                                                         ctcGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGc                                                                                              <  1:5311197/150‑1 (MQ=255)
                                                               ttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGtt                                                                                        <  1:6682693/150‑1 (MQ=255)
                                                               ttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGtt                                                                                        <  1:4557690/150‑1 (MQ=255)
                                                               ttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGtt                                                                                        <  2:1374982/150‑1 (MQ=255)
                                                               ttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGtt                                                                                        <  1:1302753/150‑1 (MQ=255)
                                                               ttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGATTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTCATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGtt                                                                                        <  1:10378139/150‑1 (MQ=255)
                                                                  gtttCTGGGCGATATGTTTGGCTATCGACGTATTTATACATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtgat                                                                                     <  1:9428834/150‑1 (MQ=255)
                                                                     tCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTAACTGTTCTGCGCCCTTTCTGATTCGCTTCAAATGCTCACCCATGCGCGTGTCATAAAAGGTTTCGGCGGTGCAGCGTTGATGAg                                                                                  >  2:101025/1‑150 (MQ=255)
                                                                      cTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGc                                                                                 <  1:9654470/150‑1 (MQ=255)
                                                                      cTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGc                                                                                 <  2:4522729/150‑1 (MQ=255)
                                                                        gggCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGt                                                                               <  2:5798048/150‑1 (MQ=255)
                                                                         ggCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGtt                                                                              >  2:6363517/1‑150 (MQ=255)
                                                                            gATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAAt                                                                           <  2:11030150/150‑1 (MQ=255)
                                                                            gATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAAt                                                                           <  2:8249418/150‑1 (MQ=255)
                                                                            gATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTCTGTTGTCTTCACTGTTCTGCGCCCGTTCTGATTCGCTGCAAAAGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAAt                                                                           >  2:4871562/1‑150 (MQ=255)
                                                                            gATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTCTGTTGTCGTAACTGTTAAGCGCCCGTTCTGATTCGCAGCAAAATCTCACCCATGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATTAGCGTTAAt                                                                           >  1:6378341/1‑150 (MQ=255)
                                                                              tatGTTTGGCTATCGACGTATTTATAACTGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATAc                                                                         <  1:4066697/150‑1 (MQ=255)
                                                                                tGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCg                                                                       >  2:5440126/1‑150 (MQ=255)
                                                                                      gCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTa                                                                 <  1:5552452/150‑1 (MQ=255)
                                                                                        tATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATc                                                               >  2:672038/1‑150 (MQ=255)
                                                                                           cGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc                                                            <  1:10046793/150‑1 (MQ=255)
                                                                                           cGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc                                                            <  1:1739965/150‑1 (MQ=255)
                                                                                           cGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc                                                            <  2:8813181/150‑1 (MQ=255)
                                                                                           cGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGAGTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc                                                            <  1:2425864/150‑1 (MQ=255)
                                                                                            gACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGcc                                                           <  2:1569850/150‑1 (MQ=255)
                                                                                             aCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCt                                                          <  1:10699448/150‑1 (MQ=255)
                                                                                             aCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCt                                                          <  2:2251721/150‑1 (MQ=255)
                                                                                             aCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCt                                                          >  2:2588301/1‑150 (MQ=255)
                                                                                             aCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACAGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCGTGCGCGTGTCATACAAGGTTTCGGCGGTGCATCGTTGATGAGCGTTAATACCGCACTTATCCGCCt                                                          >  2:10368345/1‑150 (MQ=255)
                                                                                                tATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGAt                                                       <  1:2588677/150‑1 (MQ=255)
                                                                                                tATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGAt                                                       <  1:4404390/150‑1 (MQ=255)
                                                                                                tATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGAt                                                       <  2:9583159/150‑1 (MQ=255)
                                                                                                tATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTAGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGAt                                                       <  2:6928988/150‑1 (MQ=255)
                                                                                                     ataAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATc                                                  <  2:10005638/150‑1 (MQ=255)
                                                                                                        aaTGCGGTCTGGTCGTTTTTATGTTGTCATAACTTTTATTCGCCCTTTAATATTATCTGAAAATGCTAAACCATGCGCGTTGCATAAAATGTTTAGGCGGTGCAGCGTTGATTATCGTTAATAACGCAATTATCCGCCTTATATATAcaa                                               >  2:3551867/1‑149 (MQ=255)
                                                                                                            cGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACg                                           <  2:2002607/150‑1 (MQ=255)
                                                                                                             ggTCTGGTCGTTTTTCTGTTTTCTTCACTGTTATGCCCCCAATCAGATTCGCAGCAAAATCTAACCCATGCCCGTGTCATAAAAGGTTTCGGCGGTGCACCGTTGATGAGCGTTAAAAACCCACATATAACCCAGATCTAAACACaaact                                          >  2:7494634/1‑147 (MQ=255)
                                                                                                                   gTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTg                                    <  2:3418077/150‑1 (MQ=255)
                                                                                                                    tCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTgg                                   <  1:10250305/150‑1 (MQ=255)
                                                                                                                    tCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTAGCTGCAAATGCTCACCCTTGCGCGTGTCATACTAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCTCTTATCAGCCTGATCTATCCACAACGTTTTCTgg                                   >  2:9010148/1‑150 (MQ=255)
                                                                                                                        ttttCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTag                               <  1:2913327/150‑1 (MQ=255)
                                                                                                                          ttCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTagag                             <  1:10857768/150‑1 (MQ=255)
                                                                                                                               ttgtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCTCAACGTTTTCTGGGTAGTGGGAtc                        >  2:10867624/1‑149 (MQ=255)
                                                                                                                               ttgtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATg                        <  1:3406496/150‑1 (MQ=255)
                                                                                                                                 gtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATggg                      <  2:8056665/150‑1 (MQ=255)
                                                                                                                                 gtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATggg                      <  1:7597047/150‑1 (MQ=255)
                                                                                                                                  tCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGc                     >  2:3730000/1‑150 (MQ=255)
                                                                                                                                  tCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGc                     <  1:9161081/150‑1 (MQ=255)
                                                                                                                                  tCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGc                     >  2:6576475/1‑150 (MQ=255)
                                                                                                                                  tCTTCACTGGTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGc                     <  1:3606315/150‑1 (MQ=255)
                                                                                                                                    ttCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCAt                   >  1:5027954/1‑150 (MQ=255)
                                                                                                                                    ttCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCAt                   >  1:11086494/1‑150 (MQ=255)
                                                                                                                                     tCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATTGGCATa                  >  2:9094551/1‑150 (MQ=255)
                                                                                                                                      cACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATaa                 >  1:5372764/1‑150 (MQ=255)
                                                                                                                                         tGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACt              >  2:1074345/1‑150 (MQ=255)
                                                                                                                                          gTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTc             <  2:4471831/150‑1 (MQ=255)
                                                                                                                                           ttCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCg            >  2:4050052/1‑150 (MQ=255)
                                                                                                                                           ttCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCg            >  2:7905381/1‑150 (MQ=255)
                                                                                                                                             cTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGtt          <  1:8568048/150‑1 (MQ=255)
                                                                                                                                                 gcCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAtt      <  1:5870514/150‑1 (MQ=255)
                                                                                                                                                 gcCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAtt      <  1:8612439/150‑1 (MQ=255)
                                                                                                                                                  cccTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttg     >  1:6035913/1‑150 (MQ=255)
                                                                                                                                                  cccTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCCATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttg     >  1:2083477/1‑150 (MQ=255)
                                                                                                                                                   ccTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttgt    >  2:10343130/1‑150 (MQ=255)
                                                                                                                                                    cTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttgtt   <  1:72042/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTATTGTTG  >  NC_000913/1910425‑1910723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: