Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,680 T→C 8.0% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6800TC 8.0% 702.9 / 33.4 278intergenic (+287/+428)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (77/168);  new base C (3/19);  total (90/188)
Fisher's exact test for biased strand distribution p-value = 9.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829
                                                                                                                                                     |                                                                                                                                                     
  ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTaa                                                                                                                                                     <  1:1377918/150‑1 (MQ=40)
  ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTaa                                                                                                                                                     <  1:1827463/150‑1 (MQ=40)
   gTCAGACTAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                                                    <  2:1461179/150‑1 (MQ=38)
   gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTTGCGCGTATTATCAGGCCTACACCGCTTTGAAGTGCTCCACCCCGTATGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                                                    <  1:3412839/150‑1 (MQ=14)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATATGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   <  1:7495336/150‑1 (MQ=255)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  2:9681376/1‑150 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  2:296056/1‑150 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  1:2539457/1‑150 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAGAAGGCGGTAACGCCGCATCCGACATCGAACg                                                                                                                                                   >  2:4064575/1‑150 (MQ=255)
     cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                                                                                                                                  <  1:6405217/150‑1 (MQ=38)
      aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACgtc                                                                                                                                                 <  2:9480297/150‑3 (MQ=255)
      aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                                                 >  1:1601590/1‑150 (MQ=38)
       gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                                                                                                                                <  2:6602475/150‑1 (MQ=37)
       gACAAGGCGGTCACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGTAGTGCTCCACCCCGTAGGTCGGAGAAGGGGGTGACGCCGCATCCGACATCCaagggcg                                                                                                                                                >  1:9443989/1‑145 (MQ=11)
         cAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGAATCCGAAATCTAACGCCCaa                                                                                                                                              <  1:4949413/150‑1 (MQ=25)
           aGGCGGTCCCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                            <  2:4815970/150‑1 (MQ=21)
             gCGGACACCCCGCATCCGACATCCAACCCCCGAGCCGCTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCAGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          <  2:10503677/150‑1 (MQ=11)
              cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                         <  2:9339807/150‑1 (MQ=34)
              cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCAGACATCTAACGCCCAAGCCgg                                                                                                                                         <  1:8909985/150‑1 (MQ=21)
               ggTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTGGCAGGCCTACGCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGCTTACGCCGCATCCGACATCTAACGCCCGAGCCGGt                                                                                                                                        >  2:10030391/1‑150 (MQ=11)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                      <  1:10646802/150‑1 (MQ=33)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                      >  1:6397481/1‑150 (MQ=33)
                   aCGCCGCATCCGACATCCAACGCCCGTGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  2:4626797/150‑1 (MQ=17)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCTGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:7545340/150‑1 (MQ=17)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                                                 <  2:8055038/137‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                                                 <  2:5369740/137‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                                                 >  1:5369740/1‑137 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:431071/150‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:6667072/150‑1 (MQ=32)
                   aCGCAGCAGCCGACATCCAACGCCCGAGCCGGGTGCGTTATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:5068471/150‑1 (MQ=11)
                    cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   <  2:2036957/150‑1 (MQ=32)
                     gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                              <  1:6261207/138‑1 (MQ=32)
                     gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                                                                                                                                             >  2:368980/1‑139 (MQ=32)
                     gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                                                                                                                                             <  1:368980/139‑1 (MQ=32)
                     gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                                                  <  1:8149086/150‑1 (MQ=32)
                     gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                                                  <  1:2827790/150‑1 (MQ=17)
                     gcagcATACGACATCCAACGCCCGTGCGGTTTGCCTGATTCGACGCTGGCGCGTCTTATGTGGGCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                                                  <  2:10941971/147‑1 (MQ=11)
                      cgtcATCCGAAATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:4187390/147‑1 (MQ=12)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGa                                                                                                                                 <  2:6382890/150‑1 (MQ=12)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:5975627/150‑1 (MQ=32)
                       cgcATCCGACATCGAACGCCCGTGCCTGTTGACTGTTGCGTGGCTGTCGCGTCTTTTCTGGCCTACTCCGCTGTTAAGTTCTCCACCCCGTAGGTCGGAAAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  1:2052195/150‑1 (MQ=11)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  2:2650983/150‑1 (MQ=32)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  1:5152204/150‑1 (MQ=32)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  2:4121347/150‑1 (MQ=32)
                        gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGTCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                               <  1:7898963/150‑1 (MQ=17)
                        gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                               <  1:5218902/150‑1 (MQ=32)
                        gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                               <  2:7034469/150‑1 (MQ=32)
                        gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                               <  1:8390543/150‑1 (MQ=32)
                        gcATCCAACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                               <  1:6645841/150‑1 (MQ=17)
                            ccGACATACAACGCCCGAGCCGGTTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGAAAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc                                                                                                                           <  2:678106/150‑1 (MQ=255)
                             cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                                                    <  1:9301489/124‑1 (MQ=32)
                             cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                                                    >  2:1826743/1‑124 (MQ=32)
                                cATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                                                       <  2:10260756/150‑1 (MQ=32)
                                cATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                                                       <  2:3675178/150‑1 (MQ=32)
                                 aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGcgcc                                                                                                                      <  2:1059345/150‑4 (MQ=17)
                                  tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                     <  2:6115303/150‑1 (MQ=17)
                                  tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                     <  1:11025027/150‑1 (MQ=17)
                                   ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                >  2:230467/1‑138 (MQ=32)
                                   ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  1:230467/138‑1 (MQ=32)
                                   ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                    <  1:4062527/150‑1 (MQ=32)
                                   ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                    <  1:10215284/150‑1 (MQ=32)
                                   ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                    <  2:7931643/150‑1 (MQ=17)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   >  2:5108253/1‑150 (MQ=17)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                       <  1:6879354/129‑1 (MQ=32)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                  >  2:1374208/1‑150 (MQ=17)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  2:8822736/150‑1 (MQ=17)
                                       cGCCCGAGCCGGTTGCGGGATGGGACGCTGGCGCGGCGGAACTGCGCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCTGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                <  2:5204577/150‑1 (MQ=11)
                                       cGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                <  1:3969116/150‑1 (MQ=32)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                               <  2:7616723/150‑1 (MQ=31)
                                         cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                              <  1:4531578/150‑1 (MQ=11)
                                                                                                               cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACCGATGTGAAGTGCTCACCACAGTAGTTAGGATAAGTCGGTTACGCAGCATCCGACAt                                        >  1:8707401/1‑150 (MQ=11)
                                                                                                                    gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                   >  1:9487167/1‑150 (MQ=34)
                                                                                                                    gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGCTGTGACGAGCTCCGCCCCGGAGGATGGATAAGTCGGTTACGCCGCATCCGACATCCAAc                                   >  1:7031733/1‑150 (MQ=11)
                                                                                                                     tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCAAAAGACGGTAGCCTGATGCGACGCTGGCGCGTCTTGTCAGGCCTACACCGCTGTGAAGGGCTCCACCCCGTAGGTTGGATGAGGCGGTTACGCCGCATCCGACATCCAGCg                                  >  2:7846255/1‑150 (MQ=11)
                                                                                                                       ggATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACgac                                >  2:8719395/1‑148 (MQ=21)
                                                                                                                       ggATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTc                                >  1:6146416/1‑150 (MQ=35)
                                                                                                                          caaGGCTGTTACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  2:2530721/149‑1 (MQ=255)
                                                                                                                          caaGGCGGTCACGCCGCATCCGACATCCAACGCCCTAGCCTGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  2:9629074/149‑1 (MQ=11)
                                                                                                                             ggCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                          <  1:4831502/150‑1 (MQ=37)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         >  2:7538977/1‑150 (MQ=37)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCg                         >  2:4321339/1‑150 (MQ=21)
                                                                                                                               cGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGCGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCgg                        >  2:8547385/1‑150 (MQ=37)
                                                                                                                                ggTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                       <  2:4692645/150‑1 (MQ=37)
                                                                                                                                ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCGACGTCCGAGCCGGt                       >  2:9245972/1‑150 (MQ=255)
                                                                                                                                  ttattccgcAACCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                     <  1:2744992/145‑1 (MQ=11)
                                                                                                                                   tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                    <  2:6398241/150‑1 (MQ=37)
                                                                                                                                   caCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                    <  2:7296283/149‑1 (MQ=18)
                                                                                                                                    aCGCCGCCTCCGACATCCACCGTCCGAGCCGGTTGCCCGACGGGACGCTGTCGCGTCTTTACTTGGCTACACCGCATTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                   <  2:8311164/150‑1 (MQ=11)
                                                                                                                                    aCGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                   <  1:6056841/150‑1 (MQ=255)
                                                                                                                                       ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                <  2:4961246/150‑1 (MQ=37)
                                                                                                                                       ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGGCCTACACCGCTGTGAAGTGCTACACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                >  1:3464848/1‑150 (MQ=255)
                                                                                                                                        cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt               <  2:9028809/150‑1 (MQ=37)
                                                                                                                                        cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt               <  2:5509358/150‑1 (MQ=37)
                                                                                                                                                 cATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTTTTTTGAGGCCTTCACGGCTGTGAAGTGCTCCACCCCGTAGGTTGGATATGGCGGTTACGCCGCATCCGACATCAAACGACCTAGCCGGTTGCCTGATGCGACGCTg      <  1:4679518/150‑1 (MQ=11)
                                                                                                                                                  aTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGGCCGAGCCGGTTGCCTGATGCGACGCTgg     >  2:8633201/1‑150 (MQ=12)
                                                                                                                                                    cTACCGCCCGCGCCGTGTGCCAGATGCGACGCTGGCGCGACTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg   <  2:5949650/150‑1 (MQ=11)
                                                                                                                                                    cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg   >  2:6948779/1‑150 (MQ=37)
                                                                                                                                                    cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg   <  1:6808663/150‑1 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  <  1:3533472/150‑1 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCAACAACGCGGGGAGGTCCTCCACCCCGTAGGTTGGATAGGGCGGTTACGCCGCATCCGTCGTCGCCCGACCAAGCCGGTTGCCTGATGCGGCGCGGgcgc  >  2:5442594/1‑150 (MQ=11)
                                                                                                                                                     |                                                                                                                                                     
TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: