Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,688 A→G 9.9% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6880AG 9.9% 532.5 / 40.7 237intergenic (+295/+420)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (72/124);  new base G (5/17);  total (78/159)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

ACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304539‑2304837
                                                                                                                                                      |                                                                                                                                                     
aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                                       >  1:4860896/1‑150 (MQ=35)
  aaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                                                                                                                                                     >  2:4531578/1‑150 (MQ=34)
  aaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                                                                                                                                                     >  2:9550065/1‑150 (MQ=34)
  aaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCGTCTCAGGCCTACACCGCGGGGACGTGCTCCACCCCGGAGGGCGGAGTAGGCGGGGCCGCCGCAGCCGACATCGAACGCCCGAg                                                                                                                                                     >  2:6457283/1‑150 (MQ=11)
   aGGCGGTCCCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  2:4815970/150‑1 (MQ=21)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  1:167923/150‑1 (MQ=34)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  2:9027323/150‑1 (MQ=34)
     gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  >  2:871547/1‑150 (MQ=34)
     gCGGTCACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  >  2:7932992/1‑150 (MQ=17)
      cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTTAAGTGCTCCACCCCGTTGGTCGGATAAGGCGTTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                                 <  1:4064575/150‑1 (MQ=255)
      cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                                 <  2:9339807/150‑1 (MQ=34)
      cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                                 <  1:8273026/150‑1 (MQ=34)
      cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                                 <  2:8543209/150‑1 (MQ=34)
        gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               >  2:7773814/1‑150 (MQ=34)
         tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                              <  1:10646802/150‑1 (MQ=33)
         tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                              >  1:6397481/1‑150 (MQ=33)
          cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTATGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                             <  1:7711053/150‑1 (MQ=17)
           aCGCCGCATCCGACATCCAACGCCCGTGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  2:4626797/150‑1 (MQ=17)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:431071/150‑1 (MQ=32)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  2:9172087/150‑1 (MQ=32)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:7545816/150‑1 (MQ=32)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:6667072/150‑1 (MQ=32)
           aCGCAGCAGCCGACATCCAACGCCCGAGCCGGGTGCGTTATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:5068471/150‑1 (MQ=11)
            cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                           <  2:2036957/150‑1 (MQ=32)
            cgccgcATCCGACAGCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCAGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTACCGCCCAAGCCGGTTGCCt                                                                                                                                           <  2:5426733/150‑1 (MQ=12)
             gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                                      <  1:6261207/138‑1 (MQ=32)
             gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                                      >  2:6261207/1‑138 (MQ=32)
             gcagcATACGACATCCAACGCCCGTGCGGTTTGCCTGATTCGACGCTGGCGCGTCTTATGTGGGCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                                                          <  2:10941971/147‑1 (MQ=11)
              ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                         <  1:5975253/150‑1 (MQ=32)
              ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                         <  1:2539376/150‑1 (MQ=32)
               cgcATCCGACATCGAACGCCCGTGCCTGTTGACTGTTGCGTGGCTGTCGCGTCTTTTCTGGCCTACTCCGCTGTTAAGTTCTCCACCCCGTAGGTCGGAAAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                        <  1:2052195/150‑1 (MQ=11)
               cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                        <  2:4121347/150‑1 (MQ=32)
                gcATCCGAGATCCAACGCCCGAGCCGGTGGCCTGATTCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  2:7034308/150‑1 (MQ=255)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  1:3748416/150‑1 (MQ=17)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  1:5218902/150‑1 (MQ=32)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  2:1395292/150‑1 (MQ=32)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  1:6456561/150‑1 (MQ=32)
                gcATCCAACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  1:6645841/150‑1 (MQ=17)
                 cATCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGc                                                                                                                                      <  2:8227449/150‑1 (MQ=17)
                  aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg                                                                                                                                     <  1:11040393/150‑1 (MQ=32)
                  aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCCTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg                                                                                                                                     >  1:9866172/1‑150 (MQ=17)
                   tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                                    <  1:4022196/150‑1 (MQ=32)
                    ccGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc                                                                                                                                   <  1:10938847/150‑1 (MQ=17)
                      gACATCCAACGCCCGAGCCGGTTGCCTGTTGCGGCGCTGGCGCGTCTTATCAGGCCGACACCGCGGTGAAGTGCTCCACCCCGGAGGGCGGGTAAGGCGGTTACGCCGCCTCCGACATCGGAAGCCCAAGCCGGGTGCCGGTTGcgccgc                                                                                                                                 >  1:2898426/1‑150 (MQ=11)
                      gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                         >  2:4883830/1‑142 (MQ=32)
                        cctCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAACCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                                                               <  2:10260762/148‑1 (MQ=12)
                        cATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                                                               <  2:3675178/150‑1 (MQ=32)
                         ctcccccGCCCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                              <  2:10065794/144‑1 (MQ=11)
                         aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGcgcc                                                                                                                              <  2:1059345/150‑4 (MQ=17)
                           ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                        <  1:230253/138‑1 (MQ=32)
                           ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                        >  2:230467/1‑138 (MQ=32)
                            cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           <  2:6758836/150‑1 (MQ=32)
                            cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           >  2:5108253/1‑150 (MQ=17)
                             aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               <  1:6879337/129‑1 (MQ=32)
                             aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               >  2:6879337/1‑129 (MQ=32)
                             aaCGCCCGAGCCGGTTGCCAGATGCGACCCTGGCGCGTCTTATCAGGCCGACACCGCTGTGAAGTGCTCCCCCCCGAAGGTCGGATAAGGCGGTTACGCCGCAACCGACATCTAACCCCCAAGCCGGGTGCCAGAGGCGACGCAcgcgcg                                                                                                                          >  1:6117079/1‑150 (MQ=11)
                               cgcccgcgcccgTTGCCTGATGATACGCTGTATCGTCTTAAAAGGACTACTCCGCCGAGAAGTGCACACGGGCCGTGGTGGTATATGGCGGTTTCGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                        <  2:1805452/150‑1 (MQ=2)
                               cGCCCGAGCCGGTTGCGGGATGGGACGCTGGCGCGGCGGAACTGCGCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCTGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                        <  2:5204577/150‑1 (MQ=11)
                                gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  2:7615944/150‑1 (MQ=31)
                                 cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                      <  1:4531578/150‑1 (MQ=11)
                                                                                                       ccGTTGGTCTGTTTAGGCGGTCACTCATCTTCCGACATCTTACGCCCTAGCCGGTTGCCTGATTCTAATCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa                                                 <  1:5353176/150‑1 (MQ=11)
                                                                                                        cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACCGATGTGAAGTGCTCACCACAGTAGTTAGGATAAGTCGGTTACGCAGCATCCGACAt                                                >  1:8707401/1‑150 (MQ=11)
                                                                                                         gTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc                                               >  2:3533253/1‑150 (MQ=32)
                                                                                                          gcggTGGGATATGGCGGTCACGCCGCATCGTACATCTCACGCGCTAGACGGTTGCGTGATTCGACGCTGGCGCGTCTTATCTGGGCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATcc                                              <  2:8743935/148‑1 (MQ=11)
                                                                                                             gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                           >  1:9487158/1‑150 (MQ=34)
                                                                                                              tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                          >  2:7846934/1‑150 (MQ=34)
                                                                                                              tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                          <  2:9866172/150‑1 (MQ=34)
                                                                                                                   tAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                     <  1:7421396/150‑1 (MQ=12)
                                                                                                                   caaGGCGGTCACGCCGCATCCGACATCCAACGCCCTAGCCTGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                     <  2:9629074/149‑1 (MQ=11)
                                                                                                                   caaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                     <  2:2530924/149‑1 (MQ=2)
                                                                                                                    aaGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAg                                    >  2:4229715/1‑150 (MQ=37)
                                                                                                                    aaGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGGCCGAg                                    >  1:8041199/1‑150 (MQ=21)
                                                                                                                       gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                 >  2:3966172/1‑150 (MQ=37)
                                                                                                                       gCGGTCACGCCGCATCCGACATCTAACGCCCCCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                 <  1:4217001/150‑1 (MQ=255)
                                                                                                                         ggTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                               <  2:4692645/150‑1 (MQ=37)
                                                                                                                           ttattccgcAACCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                             <  1:2744992/145‑1 (MQ=11)
                                                                                                                            tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                            <  2:6397481/150‑1 (MQ=37)
                                                                                                                            caCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                            <  2:7296283/149‑1 (MQ=18)
                                                                                                                             aCGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                           <  1:6056841/150‑1 (MQ=255)
                                                                                                                                ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                        <  2:8582112/150‑1 (MQ=37)
                                                                                                                                ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGGCCTACACCGCTGTGAAGTGCTACACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                        >  1:3464848/1‑150 (MQ=255)
                                                                                                                                 cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt                       <  2:5509358/150‑1 (MQ=37)
                                                                                                                                 cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGACAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt                       <  1:6959191/150‑1 (MQ=255)
                                                                                                                                  gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACTCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                      <  1:5051891/150‑1 (MQ=255)
                                                                                                                                        gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACCGCTGAGAAGTGCTCCACCCCGTAGGTTGGATAAGGAGGTTACGCCGCATACGACATCAAACGAACGAGCCGGTTGCCTGATGATACGc                >  2:10524156/1‑150 (MQ=11)
                                                                                                                                          cATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTTTTTTGAGGCCTTCACGGCTGTGAAGTGCTCCACCCCGTAGGTTGGATATGGCGGTTACGCCGCATCCGACATCAAACGACCTAGCCGGTTGCCTGATGCGACGCTg              <  1:4679518/150‑1 (MQ=11)
                                                                                                                                            tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc            >  2:2376414/1‑150 (MQ=37)
                                                                                                                                             cTACCGCCCGCGCCGTGTGCCAGATGCGACGCTGGCGCGACTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg           <  2:5949650/150‑1 (MQ=11)
                                                                                                                                             cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg           <  1:6808663/150‑1 (MQ=37)
                                                                                                                                             cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCGCTGAGCAGTGCTCCACCCCGTAGGTAGGAGAAGGCGGTTACGCCGCATCCTACATCCAACGTCCGAGCCGGTTGCCAGATGCGACGCTGgcg           >  2:7337389/1‑150 (MQ=11)
                                                                                                                                              tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          <  1:3533253/150‑1 (MQ=37)
                                                                                                                                              tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCAACAACGCGGGGAGGTCCTCCACCCCGTAGGTTGGATAGGGCGGTTACGCCGCATCCGTCGTCGCCCGACCAAGCCGGTTGCCTGATGCGGCGCGGgcgc          >  2:5442594/1‑150 (MQ=11)
                                                                                                                                                aCGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGTATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt        <  1:335170/150‑1 (MQ=255)
                                                                                                                                                 cGCCCGAGCCTGTTGCCTGCTGCGACGCTGGCGCGTCTTATCATGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc       <  2:10120637/150‑1 (MQ=11)
                                                                                                                                                   cccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCGCTGTGAGGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCtg     >  2:1308253/1‑149 (MQ=255)
                                                                                                                                                    ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTcagg    <  1:10030391/150‑4 (MQ=37)
                                                                                                                                                     cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   >  1:8992559/1‑150 (MQ=37)
                                                                                                                                                      aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc  >  1:6887310/1‑150 (MQ=17)
                                                                                                                                                      aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACACCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc  >  2:2644283/1‑150 (MQ=12)
                                                                                                                                                      aaGCCGGTTGCCAGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc  >  1:8793897/1‑150 (MQ=17)
                                                                                                                                                      |                                                                                                                                                     
ACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304539‑2304837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: