Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,439 G→T 6.4% D437Y (GAC→TAC) ‡ gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4390GT 6.4% 2542.3 / 8.8 840D437Y (GAC→TAC) ‡gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (329/413);  new base T (44/5);  total (422/418)
Fisher's exact test for biased strand distribution p-value = 1.26e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 852 total aligned reads displayed.

TTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGT  >  NC_000913/2470290‑2470588
                                                                                                                                                     |                                                                                                                                                     
tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                                       >  1:6386045/1‑150 (MQ=255)
     ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAATCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATGAAAAAATAATTCAAATAAATCAAGaaaaa                                                                                                                                                  >  2:5413210/1‑149 (MQ=255)
     ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGACaaa                                                                                                                                                  >  2:730320/1‑149 (MQ=255)
      gAATATCTCAATTTTATGTGTCATTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAg                                                                                                                                                >  1:8931026/1‑150 (MQ=255)
       aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATTTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                                <  1:8175825/150‑1 (MQ=255)
         tatCTCAATTTTACGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                              <  2:10178751/150‑1 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAAAATCGCTGt                                                                                                                                          >  1:5230347/1‑150 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATAAAATATCGAttt                                                                                                                                          >  2:162349/1‑148 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAAACAAAAGCGATGt                                                                                                                                          >  2:1295304/1‑150 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAATCAACACAAAAGCGCTgg                                                                                                                                          >  2:2521139/1‑149 (MQ=255)
              cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                                                                                                         <  2:8394738/150‑1 (MQ=255)
              cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAAAAGAGATGTa                                                                                                                                         >  1:10081064/1‑150 (MQ=255)
               aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAACGATGTaa                                                                                                                                        >  2:5244373/1‑150 (MQ=255)
                aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAATCGATGTaaa                                                                                                                                       >  2:9831274/1‑150 (MQ=255)
                 ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                      <  1:3625739/150‑1 (MQ=255)
                     ttgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                                                                                                                  <  1:6093316/149‑1 (MQ=255)
                        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAATAAAATAGAGATTTAAAGCACaaat                                                                                                                               >  2:3143194/1‑149 (MQ=255)
                        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                               <  1:2710515/150‑1 (MQ=255)
                        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAACAAGACAAAAGCGATGTAAAGCACTAAg                                                                                                                               >  2:6978882/1‑150 (MQ=255)
                          tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAATAAATAAATAAAATATAGATTTAAAGCACTAAGtt                                                                                                                             >  1:7507957/1‑150 (MQ=255)
                          tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAggt                                                                                                                             >  2:3335582/1‑148 (MQ=255)
                            aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                                                                                                           <  1:5077839/150‑1 (MQ=255)
                             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                          <  2:10685293/150‑1 (MQ=255)
                             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                          <  1:3253614/150‑1 (MQ=255)
                             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                          <  2:5717511/150‑1 (MQ=255)
                                 tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCACAGAAATCAAGACAAAAGCGATGTAAACCACAAAGGTTAAAATg                                                                                                                      >  2:4496645/1‑150 (MQ=255)
                                  cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                                                                                                                     <  1:4838890/150‑1 (MQ=255)
                                   ggggAATGATATAGAGATTTAAGCAATCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                                    <  2:6154135/150‑1 (MQ=255)
                                         ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACAAGACAATATCGATGTTAAGCACGAAGTTTAAATTTAGCGCCaa                                                                                                              >  2:9352161/1‑150 (MQ=255)
                                          gATATAGAGATTTAAGCAATCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACATTCGTTTTTTTAATTTATAAAATAATTCAAAGATATCAAGACTATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                                                                                                             <  1:686057/150‑1 (MQ=255)
                                            tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                           <  1:5440130/150‑1 (MQ=255)
                                             ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                                          <  2:7422241/150‑1 (MQ=255)
                                             ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                                          <  1:7149963/150‑1 (MQ=255)
                                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                         <  2:1903626/150‑1 (MQ=255)
                                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAATCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                     >  2:6441048/1‑150 (MQ=255)
                                                    tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAATAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTAAAATTCGCGCCAATAAAGgcgcgc                                                                                                   >  2:7796928/1‑150 (MQ=255)
                                                       aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAGAAAAAAACGATTTAAAGCAAAAAGGTTAAATTTAGCGCCCAGCCTGGCGCGcaca                                                                                                >  1:1417560/1‑150 (MQ=255)
                                                        agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAATAATTAAAAAAAAAAAAGAAAAAATCCAAGTAAATCAAGAAATTTAAATTTAGCGCCCATAAAGgggcgcccca                                                                                               >  1:10893896/1‑146 (MQ=255)
                                                         gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                              <  2:9647645/150‑1 (MQ=255)
                                                         gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAAACGAAGTAAATCAAGAAGTTTAATTTTAGCGCCAAAAAAGGCGCGCCCaac                                                                                              >  1:2131152/1‑149 (MQ=255)
                                                           aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAATACAAAAGCGATGTAAAGCACTAATTTTAAATTTCGCGCCAAACATTGCGCGCACAAGCt                                                                                            >  2:4177337/1‑150 (MQ=255)
                                                             gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAAAAAAAAAATAAAAAATCGATGTAAAGCACTAAGTGTAAATTTGGCGCCAAAAAATGCGCGCACAAgaaat                                                                                          >  1:9225192/1‑146 (MQ=255)
                                                               atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGAAAAAAGCCATGTAAAGCACAAACTTTAAATTGCGCGCCAATCATTGCGCGCACAAGCTataa                                                                                        >  2:4014242/1‑150 (MQ=255)
                                                               atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAAATTATAAAAAAATTAAAAGAAAACAAAACAAAAAACAAGTAAAGAAATAAGTTTAAAATTAGCGCCAAACAAGGCGCGCACAAGCAataa                                                                                        >  1:851101/1‑150 (MQ=255)
                                                               atttatttGGAATGTATATTTCGTTCGACCTTTCCTTATACATAACAGTCCATTTTATAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCCAAGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                                        <  2:2992708/150‑1 (MQ=255)
                                                               atttatttGGAATGTATATTTCGTTCGACCATTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                                        <  2:1157287/150‑1 (MQ=255)
                                                                         aaTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATAAAAAAGAGATGTAAAGCAATAAGTTTAAATTTCGCGCCAATCATTGCGCGCACAAGCTATAATTCCAAccaa                                                                              >  2:7668282/1‑148 (MQ=255)
                                                                           tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTAAAAAAAAAATAAAATAAAAAAAGAAAAAAGCGATGTAAAACAAAAAGATTAAAATTCGCCCCAAACAAGGCGCGCCCCACCTAAAAAACCAAcaaaat                                                                            >  2:8569979/1‑145 (MQ=255)
                                                                                  ttcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACAAGACAATAGCGATGTTAAGCACTTAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcct                                                                     >  2:8128057/1‑150 (MQ=255)
                                                                                   tcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                                    <  1:1859169/150‑1 (MQ=255)
                                                                                    cgtacgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAACGATGTAAAGCACTAAGTTTAAATTTCGAGCCAATCAAGGCGCGCACAAGCTATAATACCAAACTAATATctcctca                                                                   >  2:6348554/1‑149 (MQ=255)
                                                                                        cgACCTTTGCTTATACATAACAGTCGTTTTTTTAAGTTATAAAATAATTCAAAGAAAGCAAGACAATAGCGATGTAAAGCACTAACTTTAAAATGCGCGCCAATCAAGGCGCGCACACGCGCTAATACCAACCTAAGTTctcctcctcat                                                               >  2:5937560/1‑148 (MQ=255)
                                                                                         gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCGATAATACCCACCAAATTTctcctccgcgta                                                              >  2:10849938/1‑145 (MQ=255)
                                                                                             tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAATTCTCCTCCTCTTAGAGt                                                          >  2:11089391/1‑150 (MQ=255)
                                                                                             tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAATCTATAAAAACAACCAAAATTCTCCTCCTCTTACAGt                                                          >  2:8297576/1‑150 (MQ=255)
                                                                                               tGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                        <  1:5616054/150‑1 (MQ=255)
                                                                                                    ataCATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAAAAAAGAAAAAAGCGATGTAAATCACGAAGTTTAAATTGCGCGCCAATCAAGGCGCGCACAAGCTATAATAACAACCAAAATTCTCCTCCGCATAGAGTGACgata                                                   >  2:4834764/1‑150 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                               <  1:4189061/150‑1 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                               <  1:7721588/150‑1 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                               <  2:8237430/150‑1 (MQ=255)
                                                                                                         tatcAGTCTTTTTTTTAATTTATAAAATAATTAAATGAAATCAAGACAATAGCGATTTAAATCACTAAGTTTAATTTGCTCGCCAATCATGGCGCTCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                              <  1:7908436/147‑1 (MQ=255)
                                                                                                          aaCAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAAACAAGACAAAAGCGATGTAAAGCACTAAGTTTAAAATTCGCGCCAATCAAGGCGCGCCCAAGCGATAATACCAACCTAATTTATCCTCCTCTTAGAGTGACTATAACTCCt                                             >  2:9131143/1‑150 (MQ=255)
                                                                                                               tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                        >  2:2368700/1‑150 (MQ=255)
                                                                                                               tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                        <  1:8360133/150‑1 (MQ=255)
                                                                                                                     ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTGTTAGAGTGACTATATCTCCTGTTAGAATTGc                                  >  2:2208278/1‑150 (MQ=255)
                                                                                                                     ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                                  <  2:1733847/150‑1 (MQ=255)
                                                                                                                     ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                                  <  2:6715969/150‑1 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 >  1:10758468/1‑150 (MQ=255)
                                                                                                                        tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                               >  1:3757353/1‑150 (MQ=255)
                                                                                                                          aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                             >  2:3538892/1‑150 (MQ=255)
                                                                                                                           tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                            <  2:4646002/150‑1 (MQ=255)
                                                                                                                            ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                           >  2:5031477/1‑150 (MQ=255)
                                                                                                                            ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                           >  2:2724202/1‑150 (MQ=255)
                                                                                                                            ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                           >  2:7117590/1‑150 (MQ=255)
                                                                                                                             tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                          <  2:10970561/150‑1 (MQ=255)
                                                                                                                              ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                         <  2:4358864/150‑1 (MQ=255)
                                                                                                                                aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTATAGTGACTGTATCTCCTGATAGAATTGCGGTATTGACTa                       >  1:9649363/1‑150 (MQ=255)
                                                                                                                                aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                       >  1:10652661/1‑150 (MQ=255)
                                                                                                                                  aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCAGGGCGCGCACAAGCTATAATACCAACCTAAGTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                     <  1:2843973/150‑1 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  1:3112653/150‑1 (MQ=255)
                                                                                                                                      atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt                 >  2:3692786/1‑150 (MQ=255)
                                                                                                                                       ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                <  2:8974709/150‑1 (MQ=255)
                                                                                                                                        tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGATTGACTATATCTCCTGATAGAATTGAGGAATTGACTATAAAATGc               >  2:8816145/1‑150 (MQ=255)
                                                                                                                                        tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc               <  2:10489709/150‑1 (MQ=255)
                                                                                                                                        tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc               >  1:7286244/1‑150 (MQ=255)
                                                                                                                                        tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc               >  1:996930/1‑150 (MQ=255)
                                                                                                                                        tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc               >  1:1223236/1‑150 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  1:10366865/150‑1 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  2:6798781/150‑1 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGACAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  2:9954524/150‑1 (MQ=255)
                                                                                                                                          aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc             >  2:7565189/1‑150 (MQ=255)
                                                                                                                                          aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc             >  1:4555190/1‑150 (MQ=255)
                                                                                                                                           aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt            <  2:1448549/150‑1 (MQ=255)
                                                                                                                                           aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt            >  1:9120079/1‑150 (MQ=255)
                                                                                                                                           aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt            >  1:6509509/1‑150 (MQ=255)
                                                                                                                                           aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt            >  1:3415413/1‑150 (MQ=255)
                                                                                                                                           aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAGTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt            <  1:4496645/150‑1 (MQ=255)
                                                                                                                                              aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt         <  1:11049135/150‑1 (MQ=255)
                                                                                                                                                aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc       >  1:3943352/1‑150 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    >  1:1661922/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGT  >  NC_000913/2470290‑2470588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: