Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,481,804 G→T 8.8% P125T (CCA→ACA)  emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,481,8040GT 8.8% 2676.2 / 40.8 891P125T (CCA→ACA) emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (485/281);  new base T (2/110);  total (487/404)
Fisher's exact test for biased strand distribution p-value = 2.94e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 910 total aligned reads displayed.

TATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACC  >  NC_000913/2481655‑2481953
                                                                                                                                                     |                                                                                                                                                      
 atCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGa                                                                                                                                                       <  1:10648028/150‑1 (MQ=255)
      aCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTaa                                                                                                                                                  >  1:10591443/1‑150 (MQ=255)
      aCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTaa                                                                                                                                                  <  2:8366627/150‑1 (MQ=255)
       ccccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAAc                                                                                                                                                 <  2:4287148/150‑1 (MQ=255)
        cccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACg                                                                                                                                                >  2:10836514/1‑150 (MQ=255)
             cTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGcccc                                                                                                                                           >  1:2694946/1‑150 (MQ=255)
                aaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGcc                                                                                                                                        <  2:9843334/150‑1 (MQ=255)
                 aGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCa                                                                                                                                       <  2:8082928/150‑1 (MQ=255)
                 aGTAATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAACGCCAGAGCAAATGTTCTTTTTTCGGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCa                                                                                                                                       <  1:6591187/150‑1 (MQ=255)
                  gTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCAt                                                                                                                                      >  2:10466373/1‑150 (MQ=255)
                   ttATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCAtt                                                                                                                                     <  2:772496/150‑1 (MQ=255)
                   ttATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCAtt                                                                                                                                     >  2:7641211/1‑150 (MQ=255)
                   ttATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCAtt                                                                                                                                     >  2:5802346/1‑150 (MQ=255)
                      tCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACTGCCCCGCCATTAAc                                                                                                                                 >  1:7456028/1‑150 (MQ=255)
                      tCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAc                                                                                                                                 <  2:9389119/150‑1 (MQ=255)
                        acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                               <  1:340299/150‑1 (MQ=255)
                        acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                               >  1:4992102/1‑150 (MQ=255)
                         caAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAcccc                                                                                                                              >  1:3639767/1‑150 (MQ=255)
                          aaaTATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCt                                                                                                                             <  1:6851516/150‑1 (MQ=255)
                             tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                                                                          <  2:564921/150‑1 (MQ=255)
                             tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                                                                          <  2:3165290/150‑1 (MQ=255)
                             tataACCCCCCAATATCGGCCCACATATCTGATCGATAATCACGCTCATTGACCATAATCCCAGAGCAAATGTTCTTTTATCTGGCGTATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACTGCCCCGCCATTAACCCCTTaa                                                                                                                          <  2:1717596/150‑1 (MQ=255)
                                       cAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaa                                                                                                                >  1:631065/1‑150 (MQ=255)
                                        aaTATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaaa                                                                                                               >  2:2696363/1‑150 (MQ=255)
                                           atCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAat                                                                                                            >  1:4769241/1‑150 (MQ=255)
                                             cGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAatat                                                                                                          >  2:899309/1‑150 (MQ=255)
                                               gccaaaatatCGTAGCTATAATCACGGTCATTGACCATAATGCCATATAAAATGTTATTTTTTCTGGCGTATAATTCCTTAATAATAAACTATGTGACAGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCa                                                                                                        <  2:1338502/144‑1 (MQ=255)
                                               gCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTCACAGAGGAATTAACGGCCCCGCCATGAACCCCAGAACGACTCTAAAAAATATCa                                                                                                        >  2:7338372/1‑150 (MQ=255)
                                                cccACATATGGGTGCGTTAATCAGGGTCTTTGACCTTAATGCCTGAGCAATTGTTCTTTTTTGTGGGGGATATTTCCTTAATATTAAACTCTGTGACTGTGGATTTAAGGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAg                                                                                                       <  2:9646918/150‑1 (MQ=255)
                                                 ccACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGc                                                                                                      >  1:5723445/1‑150 (MQ=255)
                                                   acaTATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGcac                                                                                                    >  1:3838605/1‑150 (MQ=255)
                                                   acaTATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGcac                                                                                                    >  2:6146579/1‑150 (MQ=255)
                                                        tCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCaa                                                                                               >  2:2938809/1‑150 (MQ=255)
                                                        tCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCaa                                                                                               >  1:7087524/1‑150 (MQ=255)
                                                          ggAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGa                                                                                             >  1:11081457/1‑150 (MQ=255)
                                                          ggAGCGATAATCAAGGTCATTGACCCTAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAATCTCTGTGAAAGTGTAATTAACGGACCCGCCATTAACCCCTGAACTACTCTAAAAAATATCAGCACATCAAGa                                                                                             >  1:10865424/1‑150 (MQ=255)
                                                             gataTAATCACGTTAATTGAAAATAATGCCATAGAAAATGTTCTTTTTTCTGGCGTATAATTCATTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTg                                                                                          <  2:7127727/147‑1 (MQ=255)
                                                               taTAATCAGGTTAATTTACAATAATGCAAGAGAAATTTTTATTTTTTCTGGGGTATAATTCATTAATAATAAACTCTGTTACAGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                                                                        <  1:7319163/149‑1 (MQ=255)
                                                               taTAATCACGTTATTTTACCATAATGCCAGATAAATTGTTCTTTTTTCTGGGTTATATTTACTTAATAATAAACTCTGTGACTGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                                                                        <  1:291240/149‑1 (MQ=255)
                                                                  aaTCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGa                                                                                     >  2:10601817/1‑150 (MQ=255)
                                                                  aaTCACGGTAATTTACCATATTGCATTAGCAATTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAAGTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGa                                                                                     <  2:9551410/150‑1 (MQ=255)
                                                                    ncACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATa                                                                                   >  1:9324157/2‑150 (MQ=255)
                                                                     cACGGTAATTTACCATAATGCCATATCAATTGTTCTTTTTTGTGGCGTATAATTCCTTAATAATAAACTCTGTGACTGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAg                                                                                  <  1:10216568/150‑1 (MQ=255)
                                                                       cGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGc                                                                                >  2:1234691/1‑150 (MQ=255)
                                                                           cATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTaca                                                                            <  2:6779504/150‑1 (MQ=255)
                                                                             ttGACAATATTGCCATAGCAAATGTTCTTTTTTCTGGCGTATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAcaca                                                                          <  2:5292881/150‑1 (MQ=255)
                                                                                aCCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAAc                                                                                                                                                 <  1:1851214/77‑1 (MQ=255)
                                                                                aCCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAAc                                                                                                                                                 >  2:1851214/1‑77 (MQ=255)
                                                                                  cATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAAt                                                                     >  1:2295579/1‑150 (MQ=255)
                                                                                  aaTAATGCCAGAGCAATTGTTATTTTTTCTGGCGGAAAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAAt                                                                     <  2:2391231/149‑1 (MQ=255)
                                                                                     aaTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa                                                                  <  2:8204264/150‑1 (MQ=255)
                                                                                     aaTGCCAGAGAAATTGTTATTTTTTCTGGCGTATAATTCATTAATAATAAACTCTGTGACAGTGGATTTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa                                                                  <  1:1411998/150‑1 (MQ=255)
                                                                                           agagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc                                                            >  2:6596544/1‑150 (MQ=255)
                                                                                           agagCAAATGTTCTTTTTTCTGGCGGATAAGTCCTGAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTCACCCCGGAACGACGCTAAAATATATCAGCACGTCAAGATTGGTCGATAGGCTACACATTAATGAAGACCGc                                                            >  2:4691359/1‑150 (MQ=255)
                                                                                               cAATTGTTATTTTTTCTGGCGTATAATTCCTTAATAATAAACTCTGTTACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                        <  2:6475069/150‑1 (MQ=255)
                                                                                               cAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                        >  1:836498/1‑150 (MQ=255)
                                                                                                 aaTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaa                                                      >  1:6496178/1‑150 (MQ=255)
                                                                                                    ttttttttttCTGGCGTATAATTCTTTAATAATAAAATCTGTTACTGTGTATTTAACGGCCCCGCAATTAACCCATTAACGACTATAAAAAATATCAGAACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaaa                                                   <  2:8844000/146‑1 (MQ=255)
                                                                                                     ttCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAg                                                  >  1:5213823/1‑150 (MQ=255)
                                                                                                       cTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTg                                                >  2:488596/1‑150 (MQ=255)
                                                                                                        ttttttCTTGCTGATAATTCCTTAATATTAAAATCTGTTACTGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCTAAAAAAAAGTGa                                               <  2:10061109/150‑1 (MQ=255)
                                                                                                        ttttttCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGa                                               >  1:4422185/1‑150 (MQ=255)
                                                                                                        ttttttCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGa                                               >  1:5321790/1‑150 (MQ=255)
                                                                                                        ttttttCGGGCGTATATTTCCTTAATATTAAACTCTGTGACCGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGa                                               <  2:3731754/150‑1 (MQ=255)
                                                                                                          ttttCTGGCGTATAATTCATTAATAAAAAACTCTGTGACTGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACt                                             <  1:1610453/150‑1 (MQ=255)
                                                                                                            ttCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGa                                           >  2:3081112/1‑150 (MQ=255)
                                                                                                            ttCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGa                                           >  1:9562017/1‑150 (MQ=255)
                                                                                                             tCTGGCGTATAATTCCTTAATAATAAACTCTGTGACAGTTGAATTAACGGCCCCGCCATTACCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaa                                          <  2:8719223/150‑1 (MQ=255)
                                                                                                              cTGGCGTATAATTCCTTAATAATAAACTCTGGTACCTTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaaa                                         <  1:9870839/150‑1 (MQ=255)
                                                                                                               tGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAg                                        >  1:10034084/1‑150 (MQ=255)
                                                                                                               tGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAg                                        >  1:10988770/1‑150 (MQ=255)
                                                                                                                    taTAATTCATTAAAAATAACCTCTGTTACAGTGTAATTAACGGCCCCGAAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTaaaa                                   <  1:9739023/149‑1 (MQ=255)
                                                                                                                    gATATTTCTTAAAAAATAAACTCTGTTACTGTGCATTTAACGGCCCCTCCATTAACCCCTGAACTACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTaaaa                                   <  1:10370468/150‑1 (MQ=255)
                                                                                                                      aatttCCTTAATAATAAACTCTGTGACTGTGGAATTAACGGCCCCGCAATTAACCCCTGCACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaat                                 <  1:8580896/147‑1 (MQ=255)
                                                                                                                       aaTTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaata                                >  2:11145512/1‑150 (MQ=255)
                                                                                                                       aaTTCATTAATAATAACCTCTGTGACCGTGCAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaata                                <  1:4024458/150‑1 (MQ=255)
                                                                                                                         ttCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaataat                              <  2:5459687/150‑1 (MQ=255)
                                                                                                                         ttCATTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaataat                              <  1:9086636/150‑1 (MQ=255)
                                                                                                                            cTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCtt                           >  1:2418979/1‑150 (MQ=255)
                                                                                                                             ttAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                          >  2:10363887/1‑150 (MQ=255)
                                                                                                                             ttAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                          >  2:4513937/1‑150 (MQ=255)
                                                                                                                             ttAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTAAAGTAAAAATAATCTTaa                         >  2:701473/1‑150 (MQ=255)
                                                                                                                              taataataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTaa                         >  2:1547833/1‑150 (MQ=255)
                                                                                                                                ataataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAAtt                       >  1:2113022/1‑150 (MQ=255)
                                                                                                                                  aataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                     >  1:8825034/1‑150 (MQ=255)
                                                                                                                                  aataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                     >  2:6030472/1‑150 (MQ=255)
                                                                                                                                   ataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                    >  1:325530/1‑150 (MQ=255)
                                                                                                                                        ctcgGTCACTGTGAAATTAACGCCCCCCCATTAAACCCCTCAACTACTCTAAAAAATATCAGCACATCAACATTGGTCGATAGGCTACACATTAATGAAGACAGCTAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAtt               <  2:4009975/150‑1 (MQ=255)
                                                                                                                                         tctGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACAACAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTTACTGAAAGTAAAAATAATCTTAAATCGCCTAtac              >  1:2582095/1‑148 (MQ=255)
                                                                                                                                           tgtgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAGGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCtt            >  2:3449112/1‑150 (MQ=255)
                                                                                                                                            gttaCAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCttt           <  2:2100911/147‑1 (MQ=255)
                                                                                                                                            gtgACCGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCttt           <  1:5940036/150‑1 (MQ=255)
                                                                                                                                            gtgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCttt           <  1:3105043/150‑1 (MQ=255)
                                                                                                                                             tgACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtg          >  2:8517808/1‑150 (MQ=255)
                                                                                                                                              taCAGTGCAATTAACGGCCCCGCCATTAACCCCTCAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt         <  2:6304339/149‑1 (MQ=255)
                                                                                                                                              gACAGTGTAATTAACGGCCCCGCAATTAACCCCTTCACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt         <  1:7893075/150‑1 (MQ=255)
                                                                                                                                                   tGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCaa    >  1:7715554/1‑150 (MQ=255)
                                                                                                                                                     gAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGt                                       >  2:1102246/1‑113 (MQ=255)
                                                                                                                                                     gAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAAcc  >  1:6179822/1‑150 (MQ=255)
                                                                                                                                                     gAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAAcc  >  1:8130491/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACC  >  NC_000913/2481655‑2481953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: