Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,639,280 C→A 15.9% E2* (GAA→TAA)  yfgM ← ancillary SecYEG translocon subunit; putative anti‑RcsB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,639,2800CA15.9% 1572.3 / 63.8 540E2* (GAA→TAA) yfgMancillary SecYEG translocon subunit; putative anti‑RcsB factor
Reads supporting (aligned to +/- strand):  ref base C (111/315);  new base A (100/2);  total (223/317)
Fisher's exact test for biased strand distribution p-value = 4.20e-45
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 546 total aligned reads displayed.

GCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAAC  >  NC_000913/2639131‑2639429
                                                                                                                                                     |                                                                                                                                                     
 cGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTcc                                                                                                                                                      <  2:9586037/150‑1 (MQ=255)
    gCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAATTTGCAcc                                                                                                                                                   >  1:10725872/1‑149 (MQ=255)
     cAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTGTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTCGCGGAAATTTCCCCGc                                                                                                                                                  >  2:4406848/1‑150 (MQ=255)
        aaTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGt                                                                                                                                               <  2:7225361/150‑1 (MQ=255)
         aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGATTAACCGCTTCTACCTGGACGTTTTCGTTAACGAAAAATTACACCCAGtc                                                                                                                                              >  2:10779051/1‑150 (MQ=255)
         aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACACCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtc                                                                                                                                              <  2:1630003/150‑1 (MQ=255)
          tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGTAAATTTACACGCGGtcc                                                                                                                                             >  2:8891882/1‑150 (MQ=255)
             aCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTGGTTTATTTGGACGCTGtgcgtg                                                                                                                                          >  1:8878296/1‑145 (MQ=255)
                  aTGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGGCGGAAATTTACACCCTGtccgtctccat                                                                                                                                     >  2:5323928/1‑148 (MQ=255)
                  aTGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTACACGCTGtccttctcctt                                                                                                                                     >  1:6394452/1‑150 (MQ=255)
                  aTGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTGGCGTAAATTTACACCCGGtccgtctcctt                                                                                                                                     >  1:391881/1‑150 (MQ=255)
                    ggCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCtccttcc                                                                                                                                   <  2:10232484/150‑1 (MQ=255)
                       tGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTGTGGTTAATTTTCACGCTGTGCTTCTacttgcttt                                                                                                                                >  2:7847732/1‑149 (MQ=255)
                         ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAAc                                                                                                                              <  1:7302540/150‑1 (MQ=255)
                         ttCCAGTAGCGCCAGACAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTATCGTAAATTTCCACGCGGTCCTTCTCCTTCGTTAAc                                                                                                                              >  1:9941555/1‑150 (MQ=255)
                          tCCAGTAGCGCCAGCCAAACAGAGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCATTGCCAATTTCAGCAAAAAAGCGATTAACCGCTTCTACCTCGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAAcc                                                                                                                             <  1:10251686/150‑1 (MQ=255)
                              gTAGCGCCAGCCAATCAGTGCGCCCACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGGCGCCTAAATGTACAAGCTGTCCTACACCGTCCCAAACCCAgg                                                                                                                         >  1:7432282/1‑149 (MQ=255)
                                 gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAAc                                                                                                                      <  1:2577460/150‑1 (MQ=255)
                                    ccagccaATCAGTGCGCCAACGCCCTAAATCACCCCAACAGCCAGTGCTTTGCCTTTTTCAGCAAAAAAGCTTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTg                                                                                                                   <  1:10997753/150‑1 (MQ=255)
                                    ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGGTGTGGTAAAATTACACCCGGTGCGTATCGATGCATAAACCAGTAAAGTg                                                                                                                   >  1:9373794/1‑150 (MQ=255)
                                       gccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCGCGGAAATTTACAAGCTGTCCGTCTCCGTCCGTTAACCAGGAACGTgcgc                                                                                                                >  2:10875193/1‑150 (MQ=255)
                                           aTCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAAt                                                                                                            <  1:4378267/150‑1 (MQ=255)
                                           aTCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAAt                                                                                                            <  2:2012737/150‑1 (MQ=255)
                                             cAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGGAACGTGCGCcaaatc                                                                                                          >  1:6228864/1‑147 (MQ=255)
                                              aGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAAATTACACCCTGGCCGTGTCCGTACTTAACCCAGGAACGTGCGCAAATgcg                                                                                                         >  1:764526/1‑150 (MQ=255)
                                               gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                                        <  1:2303348/150‑1 (MQ=255)
                                                  cgcCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCTCGTAAATTTACACGCGGTACTTGACCATACTTAAACCAGTAAAGTGCGCAAATGCGCGGc                                                                                                     >  2:2315108/1‑150 (MQ=255)
                                                     cAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTAc                                                                                                  <  2:453345/150‑1 (MQ=255)
                                                      aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGGTGGCGTAAATTTGCACCCGGTCCATCTCCATCCGTAAACCAGGAACGTGCGCAAAATCGCCGCTaac                                                                                                 >  2:5390056/1‑148 (MQ=255)
                                                        cGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCt                                                                                               <  1:7743178/150‑1 (MQ=255)
                                                         gCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTa                                                                                              <  2:6333892/150‑1 (MQ=255)
                                                           ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTGTAGTAAAATTACACCCTGTCCATCAACATCATTAAACCCCTAAAGAGGGCAAAAGCGCGGCTACGCTATc                                                                                            >  1:6754651/1‑150 (MQ=255)
                                                            cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATcc                                                                                           <  1:9120534/150‑1 (MQ=255)
                                                            cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCGGTCCGTCTCCGTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCGACGCTATcc                                                                                           >  2:1699434/1‑150 (MQ=255)
                                                             aaaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCt                                                                                          <  1:10959573/150‑1 (MQ=255)
                                                               aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgc                                                                                        <  1:693802/150‑1 (MQ=255)
                                                               aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgc                                                                                        <  1:10682395/150‑1 (MQ=255)
                                                               aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCCATCCTgc                                                                                        <  1:5625533/150‑1 (MQ=255)
                                                                aTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgcg                                                                                       <  1:2706732/150‑1 (MQ=255)
                                                                    ccccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAAc                                                                                   <  2:6601862/150‑1 (MQ=255)
                                                                     cccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGGTCGAGTAAAATTCAACCCAGTACATCTCCATACTTAAACCAGGAAAATGCGCAAAATGGCCGCGACGCGATACTGCGCAAca                                                                                  >  1:1552725/1‑149 (MQ=255)
                                                                     cccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACt                                                                                  <  1:3455294/150‑1 (MQ=255)
                                                                     cccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGGTCTCGTAAATTTACACCCAGTCCGTCTACATCCATAACCCAGTAAACTGCGCAAATGCGCGGCGACGCTCTCCAGCGCCAca                                                                                  >  2:2516960/1‑149 (MQ=255)
                                                                      ccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTGTCGTAAATTTACACGCTGTCCTTCTACTTGCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCGATCCAGCGCAACTg                                                                                 >  2:6859630/1‑150 (MQ=255)
                                                                      ccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAATTTCCACGCGGTCCTTCTCCGTCCGTAACCCACTAACCTGCGCAAATGCGCGGCTACGCGATCCAGCGCAAcag                                                                                 >  2:3385604/1‑148 (MQ=255)
                                                                       cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGc                                                                                <  2:2751514/150‑1 (MQ=255)
                                                                       cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGc                                                                                <  2:8397796/150‑1 (MQ=255)
                                                                       cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTATCCGTGCTTAAACCAGTAACGTTCGCAAAAGCGCGGCTAAGCAATACTGCGCAACAGc                                                                                >  2:9123652/1‑150 (MQ=255)
                                                                          cAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGt                                                                             <  1:5290055/150‑1 (MQ=255)
                                                                            gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                                           <  2:616532/150‑1 (MQ=255)
                                                                            gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                                           <  1:328081/150‑1 (MQ=255)
                                                                            gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAATTTACACCCGGTCCGGCTCCATCCATAAACCACGAAACTGCGCCAAATCGCGGCGACGCGATCCAGCGCCACAGCCCttt                                                                           >  2:1164956/1‑150 (MQ=255)
                                                                             ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGGAAATTTGAACCCGGTCCATATCCGTACATAAACCACTAACCTTCGCAAAAGCCCGGCGACGCTATCCAGCGCAACAGCCGTTTg                                                                          >  2:1046847/1‑150 (MQ=255)
                                                                                 tGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCa                                                                      <  1:5001113/150‑1 (MQ=255)
                                                                                  gCTGTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAc                                                                     <  1:10417244/150‑1 (MQ=255)
                                                                                    tttGCCATTATCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCa                                                                   <  1:6590265/150‑1 (MQ=255)
                                                                                       gCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCagag                                                                <  2:5210674/150‑1 (MQ=255)
                                                                                         cATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAgcg                                                              <  1:5153371/150‑1 (MQ=255)
                                                                                           ttttCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCa                                                            <  1:12706/150‑1 (MQ=255)
                                                                                             ttCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCaaa                                                          <  1:5394805/150‑1 (MQ=255)
                                                                                              tCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTAGTAAATTTCCACCCTGTCCTTCTCCGTCCTTAAAACAGTAAACTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACAGCCGTTTGCTCACCACAGCGCaaaa                                                         >  2:8022861/1‑149 (MQ=255)
                                                                                                aGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTACACGCTGTCCTTCTCCTTCCATAAACCAGTAACGTGCGCAAATGCGCGGCTACGCTAAACTGCGCAACATCCGTTTTCTCACCAGAGCGCaaaaac                                                       >  2:3594506/1‑147 (MQ=255)
                                                                                                 gCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCt                                                      <  2:6747850/150‑1 (MQ=255)
                                                                                                  cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCtt                                                     <  2:970534/150‑1 (MQ=255)
                                                                                                   aaaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                                    <  1:8355074/150‑1 (MQ=255)
                                                                                                    aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCa                                                   <  2:9384911/150‑1 (MQ=255)
                                                                                                    aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCa                                                   <  2:1489103/150‑1 (MQ=255)
                                                                                                    aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCa                                                   <  2:5029905/150‑1 (MQ=255)
                                                                                                    aaaaaGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGTGGTAAATTTACAAGCAGTCCTTGTCCTTACATAAACCAGAAAAGTTCGCAAAAGCGCGGCTACGCAATCCTGCGCAACAGCCGTTTGATCAACCCAGCCCAAAACCCTCa                                                   >  2:7541135/1‑150 (MQ=255)
                                                                                                           gTTTAACCGCTTCTACCTGGTCGTTTTGGTTTTCGTAAATTTCCACGCTTTACTTATCCTTACTTAACCCAGTAACGTTGGCAAATTCGCGGCTAAGCTATACTGCGCAACTGGCGTTTTCTAACCAGTGCGCAAATCCTTAACAActgc                                            >  2:698996/1‑150 (MQ=255)
                                                                                                            tttAACCGCTTCTACCTGGTCGTGTTAGTTCTCGTAAATTTACACGCTGTCCGTCTACATCCATAACCCAGTAAAGTGCGCAAAATCGCGGCTACCCAATACTGCGCAAAAGCCGTTTTCTCACCAGAGCGCAAATCCTTAACAAatgct                                           >  2:9002645/1‑150 (MQ=255)
                                                                                                              tAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgt                                         <  1:4749911/150‑1 (MQ=255)
                                                                                                               aaCCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgtg                                        <  1:8017183/150‑1 (MQ=255)
                                                                                                               aaCCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgtg                                        <  1:9729976/150‑1 (MQ=255)
                                                                                                                   gCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGt                                    <  2:5752782/150‑1 (MQ=255)
                                                                                                                     ttCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                                  <  2:5938153/150‑1 (MQ=255)
                                                                                                                       cttccTGGTCGTTTTCGTTCTCGTATATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                                <  1:10019908/147‑1 (MQ=255)
                                                                                                                       cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                                <  1:3686924/150‑1 (MQ=255)
                                                                                                                          ccTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc                             <  2:8210025/150‑1 (MQ=255)
                                                                                                                          ccTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc                             <  2:3746071/150‑1 (MQ=255)
                                                                                                                           cTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACCCTATCCAGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACt                            >  2:7317939/1‑150 (MQ=255)
                                                                                                                            tGGTCGTTTTCGTTATCGTAAATTTACAACCTGTCCTTCTCCTTCCATAACCCACTAACGTGCGCAAAAGCGCGGCTACGCTATACAGCGCAACATCCCTTTGCTCACCAGAGCGCAAATCCTAAACAAAAGCTGTTCCGTTAGCCAcat                           >  2:6017371/1‑148 (MQ=255)
                                                                                                                             ggTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc                          <  1:1413653/150‑1 (MQ=255)
                                                                                                                             ggTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc                          >  2:9271233/1‑150 (MQ=255)
                                                                                                                              gTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCa                         <  1:2441064/150‑1 (MQ=255)
                                                                                                                              gTCGTTTTCGTTCTCGTAAATTTACACGCTGTACTTCTACTTACTTAACCCAGTAACGTGCCCAAAATCGCCGCTACGCTATCCATCGCAACAGCCCTTTGATCACCAGATCGCAAATCCATCACTAATGCTGTTCCGTTAGCCACTACa                         >  2:4338211/1‑150 (MQ=255)
                                                                                                                                cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa                       >  2:9172630/1‑150 (MQ=255)
                                                                                                                                cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa                       <  2:10106904/150‑1 (MQ=255)
                                                                                                                                  ttttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt                     >  1:7120284/1‑150 (MQ=255)
                                                                                                                                   tttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTc                    <  1:4175827/150‑1 (MQ=255)
                                                                                                                                    ttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCa                   <  2:8589382/150‑1 (MQ=255)
                                                                                                                                        ttCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa               <  1:6031459/150‑1 (MQ=255)
                                                                                                                                        ttCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa               >  1:7340399/1‑150 (MQ=255)
                                                                                                                                          ctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGc             >  2:4737967/1‑150 (MQ=255)
                                                                                                                                             gTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcacc          >  2:8830843/1‑150 (MQ=255)
                                                                                                                                              tAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcacca         >  1:9580114/1‑150 (MQ=255)
                                                                                                                                              tAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcacca         <  2:3097083/150‑1 (MQ=255)
                                                                                                                                               aaaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                                            <  2:3935925/82‑1 (MQ=255)
                                                                                                                                                   ttCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCa    >  2:1067167/1‑150 (MQ=255)
                                                                                                                                                     ccACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAAc  <  1:1644829/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAAC  >  NC_000913/2639131‑2639429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: