Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,844 G→T 5.4% intergenic (‑98/+225) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8440GT 5.4% 1983.3 / 15.8 579intergenic (‑98/+225)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base G (195/333);  new base T (49/2);  total (244/335)
Fisher's exact test for biased strand distribution p-value = 9.72e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 589 total aligned reads displayed.

GAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCG  >  NC_000913/2725695‑2725993
                                                                                                                                                      |                                                                                                                                                     
gaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTTTTTTTTTTTTAAAGGAAATATTATAAAATATTTGAATATAATTATg                                                                                                                                                       >  1:10352317/1‑150 (MQ=255)
  gTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCaa                                                                                                                                                    <  2:601654/150‑1 (MQ=255)
    aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAc                                                                                                                                                   <  1:8574851/150‑1 (MQ=255)
    aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAc                                                                                                                                                   <  2:8111109/150‑1 (MQ=255)
     tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAcc                                                                                                                                                  <  2:7591464/150‑1 (MQ=255)
     tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAcc                                                                                                                                                  >  2:3990365/1‑150 (MQ=255)
     tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCATAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAcc                                                                                                                                                  <  1:1589054/150‑1 (MQ=255)
        ctactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTa                                                                                                                                               <  2:634282/150‑1 (MQ=255)
          actTGTCAGTTTGCTGTCTGCAGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                                             <  1:707918/150‑1 (MQ=255)
             tGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTTTTGCATATCATTATGAAACCTTAACCaa                                                                                                                                          >  1:8400751/1‑149 (MQ=255)
               tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTTCATATAATTATTCAAACTTAACCAAGa                                                                                                                                        >  2:5076453/1‑150 (MQ=255)
               tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                                                                                                                        <  2:11147013/150‑1 (MQ=255)
                cAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGaa                                                                                                                                       <  1:1791609/150‑1 (MQ=255)
                   tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAttt                                                                                                                                    >  2:2771769/1‑150 (MQ=255)
                    ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTACCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                   <  2:10630990/150‑1 (MQ=255)
                       cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtt                                                                                                                                <  1:9583587/150‑1 (MQ=255)
                       cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTTAATGAAAAATTATAAATTTTTTTCATATAATTATTAAAACATAAACAAGAatttattt                                                                                                                                >  1:6005371/1‑150 (MQ=255)
                        tGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTGTTAATGAAATATTATAAAATTTTTTGAAAAAAATATTCAACCATAACAAATAATttagttag                                                                                                                              >  1:2398467/1‑150 (MQ=255)
                           cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTTGTAATGTAAAATTATAAAATTGTTTCATATCAATATGCAACCATAAACAATAAATTATTTATCa                                                                                                                            >  1:2580140/1‑150 (MQ=255)
                                gTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTGTAAAGGAAATATTATACATTTTTTTCATATAATTATGCAACCATAAACAAGAAATTAGTTAGCAGGaaa                                                                                                                       >  1:663056/1‑150 (MQ=255)
                                  tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                                                     <  1:8866615/150‑1 (MQ=255)
                                     aGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGt                                                                                                                  <  1:5866271/150‑1 (MQ=255)
                                      gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGtt                                                                                                                 <  1:9736914/150‑1 (MQ=255)
                                       tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTTTAAAGGAAATATTATAAATTTTTTTGATATAAATATTCAAACAAAACAATGAATTTTTTTTGAAGGGAAATGGtgt                                                                                                                >  1:9390327/1‑148 (MQ=255)
                                        aCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAt                                                                                                               <  1:4289857/150‑1 (MQ=255)
                                         cTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                                              <  2:8808658/150‑1 (MQ=255)
                                         atttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                                              <  2:8431219/149‑1 (MQ=255)
                                          tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAATGAAATATTATACATTTTTTGCATATCATTATGCAACCATAACCATGAAATTAGTTATCAGGAAAATTTTTATTg                                                                                                             >  2:7742937/1‑150 (MQ=255)
                                          tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                             <  1:3090111/150‑1 (MQ=255)
                                            tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgag                                                                                                           <  1:10091486/150‑1 (MQ=255)
                                            tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTGTAAAGGAAATAATATAAAATTTTTGCATATCATTATGCAAACTTAACCATGAATTTATTTATCAGGAAAATTGGTATTtagg                                                                                                          >  2:9443081/1‑150 (MQ=255)
                                              aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagga                                                                                                         <  1:5570838/150‑1 (MQ=255)
                                              aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagga                                                                                                         <  1:10978016/150‑1 (MQ=255)
                                                ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGc                                                                                                       <  1:10782585/150‑1 (MQ=255)
                                                 catgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTCTTGAGGAGCt                                                                                                      >  2:11045258/1‑150 (MQ=255)
                                                  atgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTATTTAGCAGGAAAATGGTTATTTAGGAGCtt                                                                                                     >  2:6944405/1‑150 (MQ=255)
                                                    gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                   <  1:8993571/150‑1 (MQ=255)
                                                    gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                   <  2:4177593/150‑1 (MQ=255)
                                                    gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                   <  2:9531190/150‑1 (MQ=255)
                                                      catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAgg                                                                                                 <  1:7796561/150‑1 (MQ=255)
                                                       atTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATTATACATTTTTTGCATAAAATTAATCAACCGTAAACAAGTATTTATTTTGCAGGGAAATGGGTGTTTAGGGTCTGAAggg                                                                                                >  2:3832629/1‑149 (MQ=255)
                                                           tGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTATTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTCTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaaa                                                                                            <  2:6601982/150‑1 (MQ=255)
                                                            gTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAAt                                                                                           >  2:1709851/1‑150 (MQ=255)
                                                            gTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTTTTTCATATCATTATGCAACCTTAAACAAGAATTTAGTTATCAGGAAAATTGTTATTTTGGAGCTTAAGGATAAAt                                                                                           >  2:3900817/1‑150 (MQ=255)
                                                                ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                       <  2:5257807/150‑1 (MQ=255)
                                                                ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                       <  2:3158692/150‑1 (MQ=255)
                                                                 cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                                      <  1:11086373/150‑1 (MQ=255)
                                                                  tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAAGGGTATTGAGGAGCTTAAGGATAAATTTCTgg                                                                                     >  2:5486787/1‑150 (MQ=255)
                                                                     cGTAATCCGATGCTTTTGTCGGTCGGTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGGATTTCTTTATGGAACGTTTAACATGTATTTTGTTTGCTGGAAAATGGTTATTGTGGTGGTTTAGGTTATTTTTGTGGtta                                                                                  >  2:8238783/1‑148 (MQ=255)
                                                                     cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTaa                                                                                  <  1:4731419/150‑1 (MQ=255)
                                                                        aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagga                                                                               <  2:4568932/150‑1 (MQ=255)
                                                                          tCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTGAAGGATAAATTTCTGGTAaggagg                                                                             <  2:6135933/150‑1 (MQ=255)
                                                                           ccGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAggagga                                                                            <  1:638570/150‑1 (MQ=255)
                                                                              aTGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAATTGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGacac                                                                         <  2:2220684/150‑1 (MQ=255)
                                                                                  ttttGTCGGTCGCTTTTGTTTATATATATTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                     <  1:10531478/150‑1 (MQ=255)
                                                                                   tttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTTTTGCATATAATTATTCAACCTTAACCATGAATTTAGTTAGCAGGAAAATTGTTATTGAGGAGCTTAAGGATAAATTTATTGTAAGGAGGAAACGTAtt                                                                    >  2:10023141/1‑149 (MQ=255)
                                                                                      gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                                 <  2:10423055/150‑1 (MQ=255)
                                                                                        cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                               <  2:8552903/150‑1 (MQ=255)
                                                                                         ggtcgCTTTTGTTTAATTTTTTTGTAAAGGAAATAATATACATTTTTTTCATATAAATATTCAACCATAAAAAAGAATTTAGTTATCAGGAAAATTGTTATTTAGGTGCTTAAGGTTAAATTTATTGTAAGGATGACAAGTATGGaatgt                                                              >  2:2349120/1‑147 (MQ=255)
                                                                                          gtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                                             <  2:5845620/150‑1 (MQ=255)
                                                                                            cgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGc                                                           <  1:3270693/150‑1 (MQ=255)
                                                                                              cTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATTCAACCATAACCATGAATTTAGTTAGCAGGAAAAAGGTGATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATTGAAGTGGGCac                                                         >  1:5148043/1‑149 (MQ=255)
                                                                                                    tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                   >  2:558400/1‑150 (MQ=255)
                                                                                                    tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                   >  1:1337607/1‑150 (MQ=255)
                                                                                                     ttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGTACACGTATGGAAGTGGGCAAGTTgggg                                                  <  1:8740903/150‑1 (MQ=255)
                                                                                                     ttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgggg                                                  >  1:4593492/1‑150 (MQ=255)
                                                                                                       atttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGa                                                 >  2:7924183/3‑150 (MQ=255)
                                                                                                         ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                               <  2:294360/150‑1 (MQ=255)
                                                                                                          ttttttttTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCTTTAATTTAGTTAGCAGGAAAATGGTTATTTAGGATATTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                              <  2:1345811/150‑1 (MQ=255)
                                                                                                          tttttttGTAAATGAAATATTATACATTTTTTGCATATCATTATGCAACCTTAAACATGAATTTATTTATCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAATGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                              >  1:4891720/1‑150 (MQ=255)
                                                                                                          tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                              <  2:4734825/150‑1 (MQ=255)
                                                                                                          tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                              <  2:10214439/150‑1 (MQ=255)
                                                                                                            tttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCg                                            >  1:8004497/1‑150 (MQ=255)
                                                                                                              tttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTa                                          <  1:2104539/150‑1 (MQ=255)
                                                                                                                tGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATc                                        <  1:4153860/150‑1 (MQ=255)
                                                                                                                   aaaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTGGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGt                                     >  2:2618843/1‑150 (MQ=255)
                                                                                                                   aaaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGt                                     <  1:3421825/150‑1 (MQ=255)
                                                                                                                    aaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt                                    <  1:9208575/150‑1 (MQ=255)
                                                                                                                     atgAAATATTATACATTTGTTGCATATCATTATTCAACCTTAACCATTAATTTAGTTATCAGGTAAATGGTTATTGAGGTGCTTAATGATAAATTTCTTGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGGTg                                   <  1:2896686/148‑1 (MQ=255)
                                                                                                                     aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                                   <  2:7415878/150‑1 (MQ=255)
                                                                                                                      ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                  <  2:2003104/150‑1 (MQ=255)
                                                                                                                       gAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCt                                 >  1:5310082/1‑150 (MQ=255)
                                                                                                                           tattatACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAAt                             <  2:728225/150‑1 (MQ=255)
                                                                                                                             ttatACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCt                           <  2:4960806/150‑1 (MQ=255)
                                                                                                                                    tttGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCatat                    >  1:2264902/1‑150 (MQ=255)
                                                                                                                                    tttGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCatat                    >  1:7592102/1‑150 (MQ=255)
                                                                                                                                      tgttgCATATCGTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtgt                  <  2:10747246/150‑1 (MQ=255)
                                                                                                                                       gttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTCTTGAGGAGCTTAAGGATAAATTTCTGGTCAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtgtg                 >  1:408979/1‑150 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                >  1:676705/1‑150 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                <  1:3751336/150‑1 (MQ=255)
                                                                                                                                         tgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGACGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTggg               >  2:1562236/1‑150 (MQ=255)
                                                                                                                                          gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa              <  2:3353196/150‑1 (MQ=255)
                                                                                                                                           cATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAAGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAg             >  1:7396070/1‑150 (MQ=255)
                                                                                                                                             tatCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGta           <  2:6275617/150‑1 (MQ=255)
                                                                                                                                             tatCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGta           >  2:3750975/1‑150 (MQ=255)
                                                                                                                                             tatCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGta           >  2:3591197/1‑150 (MQ=255)
                                                                                                                                               tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata         >  2:9205920/1‑150 (MQ=255)
                                                                                                                                                cATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa        >  2:4390548/1‑150 (MQ=255)
                                                                                                                                                     tGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAcgc   <  1:3658415/150‑1 (MQ=255)
                                                                                                                                                     tGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAcgc   <  1:3948655/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCG  >  NC_000913/2725695‑2725993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: