Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,070 T→A 10.1% intergenic (‑169/+98) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0700TA10.1% 1427.4 / 29.3 543intergenic (‑169/+98)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (130/336);  new base A (69/2);  total (201/342)
Fisher's exact test for biased strand distribution p-value = 3.29e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 571 total aligned reads displayed.

ATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCG  >  NC_000913/3045923‑3046219
                                                                                                                                                   |                                                                                                                                                     
attCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                                     <  1:1680114/150‑1 (MQ=255)
 ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGTGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    <  1:9776901/150‑1 (MQ=255)
 ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    <  1:4709594/150‑1 (MQ=255)
 ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTccc                                                                                                                                                    >  1:7728953/1‑150 (MQ=255)
    gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaa                                                                                                                                                 <  1:5567329/150‑1 (MQ=255)
     ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                                <  2:317368/150‑1 (MQ=255)
     ttGACGTAACTATGAGATTGCAAACAAACTGGCACAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCaaa                                                                                                                                                <  2:5190986/150‑1 (MQ=37)
        aCGTATCTTTGAGATTGCTAACAAACTGGCTCTATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGcccctttggg                                                                                                                                             <  2:9048705/150‑7 (MQ=255)
             tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                                        <  1:10055412/150‑1 (MQ=255)
               tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCGAACCCACACCCCCAAGGGGGGAggg                                                                                                                                      >  2:8507020/1‑150 (MQ=255)
               tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                      >  2:8098377/1‑150 (MQ=255)
               tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCACTACCCAAAGGGGCGAggg                                                                                                                                      >  1:298943/1‑150 (MQ=255)
               tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                      >  1:6555712/1‑150 (MQ=255)
               tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGATCCCCACACCCAAAACCACACCCCAAAACGGCGAggg                                                                                                                                      >  2:1208512/1‑150 (MQ=255)
                 tGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                                    <  1:7814165/150‑1 (MQ=255)
                   agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                                                                                                  <  1:6709721/150‑1 (MQ=255)
                   agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                                                                                                  >  1:7293680/1‑150 (MQ=255)
                         cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                            <  1:11139975/150‑1 (MQ=255)
                         cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                            <  2:5593128/150‑1 (MQ=255)
                         cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                            <  2:6478653/150‑1 (MQ=255)
                         cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                            <  2:9831393/150‑1 (MQ=255)
                          tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                                           >  2:2583601/1‑150 (MQ=255)
                          tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                                           >  1:4404799/1‑150 (MQ=255)
                          tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                                           >  1:6481501/1‑150 (MQ=255)
                           aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                                                                                          <  1:10917785/150‑1 (MQ=255)
                            aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCa                                                                                                                         <  1:7621811/150‑1 (MQ=255)
                              aaaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACTGTCTGAGCAcg                                                                                                                       >  2:7930459/1‑149 (MQ=255)
                               aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtt                                                                                                                      <  2:10780219/150‑1 (MQ=255)
                                 cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtttt                                                                                                                    <  1:3652824/150‑1 (MQ=255)
                                     cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCGAACCCACTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTAc                                                                                                                >  2:3443933/1‑150 (MQ=255)
                                        aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                             <  2:7789874/150‑1 (MQ=255)
                                           cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                                          <  1:2689597/150‑1 (MQ=255)
                                            ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGAAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCCCCCAAACCCACAACCCAAAAGGGCGAGGGGACAGTATGCGCACGATTGTTCATTGTCa                                                                                                         >  1:8318079/1‑150 (MQ=255)
                                             tCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCAt                                                                                                        <  1:4952134/150‑1 (MQ=255)
                                             tCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACACCCCCAAGGGGCGGGGGGGCTGTCTGAGCACGTTTGTACTTTGTCAt                                                                                                        >  1:1021997/1‑150 (MQ=255)
                                              cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                                       <  2:9199836/150‑1 (MQ=255)
                                               aaTGTTGGCACGCTTGTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                      <  1:8784109/150‑1 (MQ=255)
                                               aaTGTTGGCACGCTGTTCCCCCTCGCCCTTGCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                      <  1:9981471/150‑1 (MQ=255)
                                                           cTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgc                                                                                          <  2:476776/150‑1 (MQ=255)
                                                           cTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgc                                                                                          <  1:6136621/150‑1 (MQ=255)
                                                           cTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAACCTAAACCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCTGACAAAAAAGgc                                                                                          >  2:6940576/1‑150 (MQ=255)
                                                                cccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCCGTGCCGGCCTGTTACCCGCACCCAAACCCGATCCCCCAAGGGGCGCCGGGGCGTTCGGTGCACCTTTGTACTTTTTCATCAGGATAAAAACGCGCCGa                                                                                     >  2:2279854/1‑150 (MQ=255)
                                                                  cccTCTCCCTGAAAGGGCGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCTCACCCCAAAGGGGCGAGGGGGCTGTCTGAGCACGTTTGTACTTTTTAATCAGACTAAAAAGGCGCCGAAg                                                                                   >  1:10804667/1‑150 (MQ=255)
                                                                   ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCGCACCCAAAACCACTACCCCAACGGGGGGGGGGACTGTCGGAGCACGTGTTTACGTTTTAATCAGACAAAAAAAGCGCCGAAgc                                                                                  >  2:10255965/1‑150 (MQ=255)
                                                                     tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                                                <  1:3584095/150‑1 (MQ=255)
                                                                     tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCAAACCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCAGACAAAAAAGGCGCCGAAgcgc                                                                                >  2:4631187/1‑150 (MQ=37)
                                                                      cGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGcc                                                                               <  1:3233402/150‑1 (MQ=255)
                                                                      cGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGcc                                                                               <  1:2244980/150‑1 (MQ=255)
                                                                       gCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCt                                                                              <  1:3810642/150‑1 (MQ=255)
                                                                          cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                                           <  1:86048/150‑1 (MQ=255)
                                                                             tCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaa                                                                        <  2:6100825/150‑1 (MQ=255)
                                                                               aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                      <  2:1010692/150‑1 (MQ=255)
                                                                               aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                      <  2:2568313/150‑1 (MQ=255)
                                                                                gggAGCGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAAt                                                                     <  2:6540053/150‑1 (MQ=255)
                                                                                gggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAAt                                                                     >  2:8296258/1‑150 (MQ=255)
                                                                                 ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                    <  2:10580359/150‑1 (MQ=255)
                                                                                 ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                    <  1:3444047/150‑1 (MQ=255)
                                                                                    gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                                                 <  1:5710896/150‑1 (MQ=255)
                                                                                    gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                                                 <  2:4084076/150‑1 (MQ=255)
                                                                                           ggggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAg                                                          <  1:9604345/150‑1 (MQ=255)
                                                                                            gggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGt                                                         <  1:8507020/150‑1 (MQ=255)
                                                                                              gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCGAAACCACACCCCCAAGGGGCGAGGGGACTGTCGGAGCACGTTTTTACTTTGTCAACATACAAAAAAGGCGCCCAAGCGCCCTTAGAAAAAAGTCGAATCACTGa                                                       >  2:7539685/1‑150 (MQ=255)
                                                                                              gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGa                                                       <  1:4004123/150‑1 (MQ=255)
                                                                                                   ggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTTCTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTAc                                                  <  2:5879171/150‑1 (MQ=255)
                                                                                                    gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                 <  1:205060/150‑1 (MQ=255)
                                                                                                    gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                 <  2:4430385/150‑1 (MQ=255)
                                                                                                    gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                 <  1:4545636/150‑1 (MQ=255)
                                                                                                    gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACGGTCTGAGCCCGTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                 >  2:390149/1‑150 (MQ=38)
                                                                                                    gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCTGTATGAGCTCATTTGTACATTGTCATATGACTAAAAAGGCGCCGAAGCGCGTTTAGCAACAAGTCGAAACAGTGaaagact                                                 >  2:9599492/1‑145 (MQ=37)
                                                                                                     tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                                <  2:7293680/150‑1 (MQ=255)
                                                                                                     tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTGTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                                >  2:3735056/1‑150 (MQ=255)
                                                                                                       aaTCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGt                                              <  1:10475300/150‑1 (MQ=255)
                                                                                                       aaTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGt                                              <  2:4308039/150‑1 (MQ=255)
                                                                                                           ttCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTTTCAACTGACTAAAAAGGCGCCCAAGCGCCGTTAGAAAAAATTCGAATAAGTGAATTACTGGTATTc                                          >  2:3541030/1‑150 (MQ=37)
                                                                                                           ttCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTTTCATCTGACTAAAAAAGCGCCGAACCGCCTTTAGAAAAAAGTCGAAAAACTGAATTAATGGTAtta                                          >  2:7587618/1‑149 (MQ=37)
                                                                                                               cgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTa                                      <  2:3996828/150‑1 (MQ=255)
                                                                                                                gcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTaa                                     <  2:620072/150‑1 (MQ=255)
                                                                                                                 ccAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAAt                                    <  1:1169843/150‑1 (MQ=255)
                                                                                                                          gCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCa                           <  1:9492615/150‑1 (MQ=255)
                                                                                                                           ccTGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAACTAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg                          <  2:11092479/150‑1 (MQ=255)
                                                                                                                             tGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGTAGAGGGTACTGTCTGAGCTCTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTATAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCatta                        <  1:9147442/150‑4 (MQ=255)
                                                                                                                             tGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGa                        <  2:5032635/150‑1 (MQ=255)
                                                                                                                              gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAg                       <  2:5518283/150‑1 (MQ=255)
                                                                                                                                  cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                   >  2:1550069/1‑150 (MQ=255)
                                                                                                                                    cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAAc                 >  1:8128907/1‑150 (MQ=255)
                                                                                                                                       aCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAgg              <  1:7222615/150‑1 (MQ=255)
                                                                                                                                        cccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTCCTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGt             >  2:4241861/1‑150 (MQ=255)
                                                                                                                                          agtccccTCGCCCCTTTGGGGAGAGGGTACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTa           >  2:1754540/5‑150 (MQ=255)
                                                                                                                                           tAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTAc          >  1:1473012/1‑150 (MQ=255)
                                                                                                                                            aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGGGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg         >  1:592611/1‑150 (MQ=255)
                                                                                                                                            aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg         <  2:7184934/150‑1 (MQ=255)
                                                                                                                                            aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg         <  2:2997750/150‑1 (MQ=255)
                                                                                                                                            aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg         <  1:6973191/150‑1 (MQ=255)
                                                                                                                                             aCCCTCTCCCCACAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACgg        <  2:8657927/150‑1 (MQ=255)
                                                                                                                                               ccTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTGTTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTc      >  2:932309/1‑150 (MQ=255)
                                                                                                                                                ctctCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAATGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCg     >  1:5329578/1‑150 (MQ=255)
                                                                                                                                                 tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc    <  2:6649192/150‑1 (MQ=255)
                                                                                                                                                 tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc    >  2:5731587/1‑150 (MQ=255)
                                                                                                                                                 tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc    >  1:10690321/1‑150 (MQ=255)
                                                                                                                                                   tcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCg  <  1:1986090/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCG  >  NC_000913/3045923‑3046219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: