Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,609 T→G 6.8% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6090TG 6.8% 1323.5 / 32.8 457intergenic (+64/‑335)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (273/119);  new base G (3/28);  total (277/180)
Fisher's exact test for biased strand distribution p-value = 3.58e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 477 total aligned reads displayed.

TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757
                                                                                                                                                      |                                                                                                                                                    
tGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCtt                                                                                                                                                      >  1:619785/1‑150 (MQ=255)
tGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCtt                                                                                                                                                      <  2:9091176/150‑1 (MQ=255)
   gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                   <  1:8179776/150‑1 (MQ=255)
      tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAggg                                                                                                                                                >  1:7974804/1‑150 (MQ=255)
      tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAggg                                                                                                                                                >  2:1612662/1‑150 (MQ=255)
       ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                               >  1:3310127/1‑150 (MQ=255)
        gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgag                                                                                                                                              >  2:7151711/1‑150 (MQ=255)
        gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgag                                                                                                                                              >  2:4065522/1‑150 (MQ=255)
         tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCATAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTATCCCTCTCCCTTCCAGGgaga                                                                                                                                             >  1:3874722/1‑150 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           <  2:4759047/150‑1 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           <  2:10445149/150‑1 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           <  2:5452164/150‑1 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           <  2:8181696/150‑1 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                           <  2:5967611/150‑1 (MQ=255)
            ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAggg                                                                                                                                          >  2:286539/1‑150 (MQ=255)
              ggCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTc                                                                                                                                        <  2:9709946/150‑1 (MQ=255)
               gCATTCTGGTGATGACGTTTATTATCAACGCCGGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                                                                                       >  1:2561782/1‑150 (MQ=255)
                cATTCGGGTGATGACGTTTATTATCACCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                      <  1:10583798/150‑1 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 >  2:9248298/1‑150 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTACCAGGGAGAGGTTCGGGGTgg                                                                                                                                 >  1:10703872/1‑149 (MQ=255)
                      ggTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAg                                                                                                                                >  2:6987767/1‑150 (MQ=255)
                       gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                               <  2:1227173/150‑1 (MQ=255)
                         gatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGt                                                                                                                             >  2:5672262/1‑150 (MQ=255)
                          atgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                                                                                            >  2:3806099/1‑150 (MQ=255)
                             aCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                                                                                         >  1:9931610/1‑150 (MQ=255)
                             aCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                                                                                         >  1:4389315/1‑150 (MQ=255)
                              cGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttt                                                                                                                        >  2:8570020/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  2:10096652/1‑150 (MQ=255)
                                 tttttatCAAGGCGGGGGTGTATACTGGGAATGATAAGCTGGGGGGTGGATATTTTTTAATCGGCCAATGCGGTGTACCGTGTCATTAACTTGAGCACCGTTTTCCCCCTCTCCCTTCCAGGTTGTGGTTGGGGGTGAGGGTAATTTTTc                                                                                                                     <  2:1777274/147‑1 (MQ=255)
                                  tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCTCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                    >  1:3460858/1‑150 (MQ=255)
                                         aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGag                                                                                                             >  1:10009889/1‑149 (MQ=255)
                                         aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                             >  1:2257934/1‑150 (MQ=255)
                                                gggTGATTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTACGCCGTGTACCCGGCCATTAACATGAGCCCCGTTTTCTCCCTCCCCCTTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                                                                                      <  2:6639404/150‑1 (MQ=255)
                                                    gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                                                                                  >  1:10946696/1‑150 (MQ=255)
                                                    gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                                                                                  >  2:3006220/1‑150 (MQ=255)
                                                        tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                              >  1:5922178/1‑150 (MQ=255)
                                                        aaccgccATTATAACCACCGGGGTGCATAATTTTAAAACTCCCAAAGCCGTTAACCCTGTAATTAACATGCGCACCGTTTACACCCTCACCCTACCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                              <  2:7415566/144‑1 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  2:4849335/1‑150 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  2:6056252/1‑150 (MQ=255)
                                                                tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                                                                                      >  2:1848951/1‑150 (MQ=255)
                                                                    aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  >  1:7459855/1‑150 (MQ=255)
                                                                    aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  >  2:2275131/1‑150 (MQ=255)
                                                                      gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCGTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctc                                                                                >  2:10945353/1‑150 (MQ=255)
                                                                       cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                               >  2:1394847/1‑150 (MQ=255)
                                                                       cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                               >  1:10182886/1‑150 (MQ=255)
                                                                           ggggTGGTTTTTTTTTATTCTGCCTATGCGGTGTACCCTGTCTTTAACTTGTGCACGGTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcccc                                                                           <  1:8250893/150‑1 (MQ=21)
                                                                            gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCa                                                                          >  1:9804834/1‑150 (MQ=255)
                                                                                 tatatTTTTTAATCTGCATAAGCGTTGTACCGTTTATTTAACATTTGCAGGTTTTGCCCCCACCCCCGCCCTGGGTGTGGGGGGGGGTGCGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                     <  1:7872605/149‑1 (MQ=12)
                                                                                 taTAATTTTTAATCTGCCTAAGCCTTGTACCCTTAAATTAAAATTCGCACCTTTTTCACCCCCTCCCTTCCTGGTTGAGGGTGGGGGTCTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                     <  1:3064634/149‑1 (MQ=21)
                                                                                 gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                     >  1:1570416/1‑150 (MQ=255)
                                                                                   tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                   >  2:3283557/1‑150 (MQ=255)
                                                                                    aaTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                  >  1:8113696/1‑150 (MQ=255)
                                                                                      tttttACTCTGCCTAAGCCGGGTACCCTGTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCGTCCCGGGTGTGGGTCGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                <  1:10844142/150‑1 (MQ=37)
                                                                                      tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                >  1:5775011/1‑150 (MQ=255)
                                                                                      tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                >  2:7541295/1‑150 (MQ=255)
                                                                                      tttttAATCTGACTCCGCGGTTAACCCTTTAAATACCAGGCCCACTTTTTCCCCCCACCCCCTGCCTGGGTGTGGGGGGGGGTTTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                <  1:864830/150‑1 (MQ=12)
                                                                                       ttttATCCTGCCTACGCGTTGTACCCTTAAATTAAATTGAGAACGGTTTACCCCCTCGCCGTTCCGGGTTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               <  1:1393075/150‑1 (MQ=12)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCACTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               >  2:3549506/1‑150 (MQ=255)
                                                                                        tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                              >  2:8076435/1‑150 (MQ=255)
                                                                                         ttAATCTGCCTACGCCTTTTACCCTGTCTTTAACATGAGCACGGTTTCCCCCCACCCCCTGCCCGGGTTTGGGTGGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                             <  2:195455/150‑1 (MQ=21)
                                                                                         ttAATCTGCATAAGCCGTGTACCCTGTCATTAACATGAGCACCTTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                             <  2:3706339/150‑1 (MQ=255)
                                                                                              cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTAACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTg                                                        >  2:4644090/1‑150 (MQ=255)
                                                                                                gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAc                                                      >  1:10913426/1‑150 (MQ=255)
                                                                                                   tccgCCGTTTACCGTTTAATTAACTTCCGCAGTTTTTCCTCCCCCCCCCTGCCGGGGTGGGGGGGGGGGGGCGGCCCATTTTTCGCACCGATGCTGGACTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   <  1:4993710/147‑1 (MQ=12)
                                                                                                   tacgcggtgtACCCTTACATTAAATTGAGCCCGTTTTTCCCCCTCCCCGTCCCGGGTCTGGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   <  2:4234778/144‑1 (MQ=18)
                                                                                                   tacgCCGTTTACCGTGTATTTAACATGAGCACCTTTTTCTCCCGCTCCCTTCCCGGGAGTGGTTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   <  2:571577/147‑1 (MQ=25)
                                                                                                   aacgcggtgcACCCTCTCATTAACATGACCACGGTTTTCCCCCTCCCCCTGCCCGGGTGTGGGGGGGGGTGAGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   <  1:1723814/144‑1 (MQ=18)
                                                                                                   aacgCCGTGTCCCCTCTATTAAACATCCGCCCCTTTTACCCCCCCTCCCTCCCCGGGTTTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   <  2:10304487/147‑1 (MQ=16)
                                                                                                    acgCCGTTAACCCTGACATTAACTTGCGCACCGTTGACCCCCTCCCCCGCCCTGGTTGTGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                  <  1:835820/148‑1 (MQ=18)
                                                                                                        cGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCt                                              >  1:2690036/1‑150 (MQ=255)
                                                                                                               ccTGTCATTAACATGCGCACCGATTTCGCCCTCGCCCTTCCAGGGAGAGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                       <  2:8670687/150‑1 (MQ=25)
                                                                                                               ccTGTAATTAACATGAGCACCGTTTCCACCCTCTCCCTTCCCGGGTGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                       <  1:5418528/150‑1 (MQ=25)
                                                                                                                ctgtatttAACATGCGCACCGTTTTCTCCCTCTCCCTTCCTGGGAGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                      <  2:706255/144‑1 (MQ=25)
                                                                                                                cTGTCATTAACTTGAGCCCGGTTTTCGCCCTCGCCTTTCCCGGGTGTGGGTGGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                      <  2:4398307/150‑1 (MQ=21)
                                                                                                                 tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTGGTTGAtg                                     >  1:11060319/1‑150 (MQ=255)
                                                                                                                   taaTTAACTTGCGCACGGTGTTCCCCCACCCCCTGCCTGGGAGTGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgtt                                   <  1:6094867/148‑1 (MQ=21)
                                                                                                                    cATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttg                                  >  2:9798104/1‑150 (MQ=255)
                                                                                                                     aTTAACATGAGCACGGTTTTCTCCCTCTCCCTTCCTGGGAGTGGGTCGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                 <  1:7233185/150‑1 (MQ=25)
                                                                                                                      ttAACATGAGCACGGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTGGGGTTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                <  1:6654471/150‑1 (MQ=255)
                                                                                                                       tAACTTGAGCACCTTTTTCTCCCTCTCCCTTCCCGGGTGAGGGTGGGGGGGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtca                               <  2:1429704/150‑1 (MQ=25)
                                                                                                                           cagcacACCGTTTTCCACCCCCCCCCTGCCTGGTGGTGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatca                            <  1:4801305/145‑1 (MQ=21)
                                                                                                                           aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAGCGATGCTGGGGTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGTCCGTGTGGTTTTTTTATGTTGtcatctg                           >  2:520329/1‑148 (MQ=21)
                                                                                                                           aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                           >  1:3651030/1‑150 (MQ=255)
                                                                                                                             gcccccggTTTCCCCCCTCCCCCTCCCGGGGTGTGGGGGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                         <  1:5595211/143‑1 (MQ=21)
                                                                                                                             gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                         <  2:10810975/150‑1 (MQ=255)
                                                                                                                             gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                         >  1:9418685/1‑150 (MQ=255)
                                                                                                                                cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACACCCCAAAAGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                      >  1:2316350/1‑150 (MQ=255)
                                                                                                                                  ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                    >  1:6862782/1‑150 (MQ=255)
                                                                                                                                       gtgcgcccgctccctCCCCGGGAGAGGGTCGGGGTGCGGGTAATTTGTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                <  2:6260099/145‑1 (MQ=37)
                                                                                                                                        tctccctctcccTTCCAGGGTGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa              <  1:7365145/150‑1 (MQ=255)
                                                                                                                                          gcccctgtccgtTCCTGGTTGTGGGTGGGGTTGGGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCAc             <  1:9708141/148‑1 (MQ=37)
                                                                                                                                          ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCAc             >  2:6868192/1‑150 (MQ=255)
                                                                                                                                             cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt          >  1:6456459/1‑150 (MQ=255)
                                                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         >  2:2631800/1‑150 (MQ=255)
                                                                                                                                              ctctccctTCCAGGGAGAGGCTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTATGATGTTGTCATCAGACAGGACGCCGCACGATg         >  2:6570336/1‑150 (MQ=25)
                                                                                                                                                 tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCCCGTTGGCt      >  2:9890692/1‑150 (MQ=255)
                                                                                                                                                 tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt      >  1:9059018/1‑150 (MQ=255)
                                                                                                                                                     ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGTCATCTGTCTGGAAGCCGCACGTTGGCTTTAt  >  1:7733873/1‑150 (MQ=255)
                                                                                                                                                     ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt  >  1:8768020/1‑150 (MQ=255)
                                                                                                                                                     ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTACCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCATTCTGGAAGCCGCACGTTGGCTTTAt  >  2:10681525/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: