Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,834 A→T 5.8% A336A (GCA→GCT yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,8340AT 5.8% 2221.1 / 12.7 671A336A (GCA→GCTyhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base A (325/283);  new base T (2/59);  total (328/343)
Fisher's exact test for biased strand distribution p-value = 2.52e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 676 total aligned reads displayed.

GGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGT  >  NC_000913/3368687‑3368983
                                                                                                                                                   |                                                                                                                                                     
 ggCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGt                                                                                                                                                    >  1:1991452/1‑150 (MQ=255)
  gCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGtt                                                                                                                                                   >  1:1232535/1‑150 (MQ=255)
    aaaTTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTg                                                                                                                                                 <  1:6078207/150‑1 (MQ=255)
     aaTTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGc                                                                                                                                                <  1:10697085/150‑1 (MQ=255)
     aaTTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGc                                                                                                                                                <  1:10904572/150‑1 (MQ=255)
     aaTTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGc                                                                                                                                                <  1:3458751/150‑1 (MQ=255)
        tCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTa                                                                                                                                             >  2:2954688/1‑150 (MQ=255)
         cAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTaa                                                                                                                                            <  1:5494791/150‑1 (MQ=255)
             tataGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCAGTATGCTCTGGCAGGTTTGCCTAACAcc                                                                                                                                        >  2:3133002/1‑150 (MQ=255)
              ataGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCg                                                                                                                                       >  1:10182913/1‑150 (MQ=255)
                 gCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTc                                                                                                                                    >  2:4384486/1‑150 (MQ=255)
                    aTGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTgg                                                                                                                                 <  1:3987203/150‑1 (MQ=255)
                       ccGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCaa                                                                                                                              >  1:2925107/1‑150 (MQ=255)
                         ggACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGc                                                                                                                            >  2:10652771/1‑150 (MQ=255)
                                tGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAAtata                                                                                                                     >  1:8515633/1‑150 (MQ=255)
                                tGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAAtata                                                                                                                     >  1:1645719/1‑150 (MQ=255)
                                 gAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATaa                                                                                                                    <  1:2794031/150‑1 (MQ=255)
                                 gAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATaa                                                                                                                    >  2:4076024/1‑150 (MQ=255)
                                 gAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATaa                                                                                                                    <  2:5393304/150‑1 (MQ=255)
                                   atatGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                                                                                                                  <  2:9384514/150‑1 (MQ=255)
                                   atatGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                                                                                                                  <  1:9368343/150‑1 (MQ=255)
                                   atatGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                                                                                                                  <  1:6663509/150‑1 (MQ=255)
                                       gTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGATCTGGCAAGCGAATATAAGATGCa                                                                                                              >  2:10507086/1‑150 (MQ=255)
                                        tATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCaa                                                                                                             >  2:1925070/1‑150 (MQ=255)
                                         aTCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAAc                                                                                                            >  1:5804610/1‑150 (MQ=255)
                                          tctcAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACt                                                                                                           >  1:1391286/1‑150 (MQ=255)
                                           ctcAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTa                                                                                                          >  1:4891348/1‑150 (MQ=255)
                                                 tACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCtgat                                                                                                    >  1:222262/1‑150 (MQ=255)
                                                  aCGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCtgatg                                                                                                   <  1:264331/150‑1 (MQ=255)
                                                   cGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCtgatga                                                                                                  >  1:2420713/1‑150 (MQ=255)
                                                   cGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCtgatga                                                                                                  <  1:8610135/150‑1 (MQ=255)
                                                    gCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATgag                                                                                                 >  1:5289827/1‑150 (MQ=255)
                                                    gCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATgag                                                                                                 >  2:7685623/1‑150 (MQ=255)
                                                      cAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGACGCGCCTTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaa                                                                                               >  1:4133510/1‑150 (MQ=255)
                                                      cAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaa                                                                                               <  1:3730678/150‑1 (MQ=255)
                                                       aaaGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaaa                                                                                              <  1:11102506/150‑1 (MQ=255)
                                                       aaaGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaaa                                                                                              <  1:10484228/150‑1 (MQ=255)
                                                       aaaGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaaa                                                                                              <  1:4425705/150‑1 (MQ=255)
                                                       aaaGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaaa                                                                                              >  2:10269613/1‑150 (MQ=255)
                                                          gagCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGACTATAAGATGCAACTACCTGATGAGAACCGa                                                                                           >  2:1661804/1‑150 (MQ=255)
                                                          gagCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGa                                                                                           >  1:6522707/1‑150 (MQ=255)
                                                                tGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCa                                                                                     <  2:9117799/150‑1 (MQ=255)
                                                                  gACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGa                                                                                   <  2:6550721/150‑1 (MQ=255)
                                                                  gACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGa                                                                                   <  2:6230711/150‑1 (MQ=255)
                                                                      cTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAATGGAGCCGTAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGAAAGCGAATATAATTTGCAACTACCTGATGAGAAACGACTCGCAGATGaa                                                                               <  2:1020555/150‑1 (MQ=255)
                                                                       tACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATTTAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGTAc                                                                              >  2:10852079/1‑150 (MQ=255)
                                                                       tACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAAc                                                                              >  1:9028026/1‑150 (MQ=255)
                                                                       tACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAAc                                                                              >  1:62347/1‑150 (MQ=255)
                                                                         ccGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTc                                                                            >  2:3450895/1‑150 (MQ=255)
                                                                            gATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAATGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACGGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGtt                                                                         <  2:5157899/150‑1 (MQ=255)
                                                                            gATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATACGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGtt                                                                         <  2:8246136/150‑1 (MQ=255)
                                                                            gATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGtt                                                                         <  2:2369876/150‑1 (MQ=255)
                                                                            gATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGtt                                                                         >  2:10972733/1‑150 (MQ=255)
                                                                             aTGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTc                                                                        <  2:9706623/150‑1 (MQ=255)
                                                                                aaaaTCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGTAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGaa                                                                     >  1:5626623/1‑150 (MQ=255)
                                                                                 aaaTCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAac                                                                    >  2:2059026/1‑150 (MQ=255)
                                                                                     ccGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAg                                                                >  2:6775916/1‑150 (MQ=255)
                                                                                       tcccATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTATCACCGTTCTGGCAAGCGAATATAAGTTGCAACTACCTGATGAGAAACGACTCGCTGATGTACTCGTTCGAACACAGGc                                                              >  1:8087123/2‑150 (MQ=255)
                                                                                       gCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCtga                                                                                                     <  1:876539/111‑1 (MQ=255)
                                                                                       gCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGaataaatcc                                                              >  2:8515013/1‑143 (MQ=255)
                                                                                       gCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGc                                                              >  2:1804210/1‑150 (MQ=255)
                                                                                       gCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGc                                                              >  2:3715156/1‑150 (MQ=255)
                                                                                       gCCCATCGGTCTGCTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGAAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGGTCGAACACAGGc                                                              >  2:9604817/1‑150 (MQ=255)
                                                                                        cccACCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCg                                                             >  1:10085863/1‑150 (MQ=255)
                                                                                         ccATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCgg                                                            >  1:767599/1‑150 (MQ=255)
                                                                                          cATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGt                                                           <  2:1001315/150‑1 (MQ=255)
                                                                                            tCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGc                                                         <  2:1232535/150‑1 (MQ=255)
                                                                                              ggTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCTAATATAACATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTa                                                       <  2:5659498/150‑1 (MQ=255)
                                                                                               gTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCTGGTTTGCCTAACACGGTTCTGGCAAGCGAATATAAGATGAAACTACCTGATTAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTag                                                      <  2:73370/150‑1 (MQ=255)
                                                                                               gTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGTTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTag                                                      <  2:6409822/150‑1 (MQ=255)
                                                                                                    gTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGaa                                                 <  2:6950718/150‑1 (MQ=255)
                                                                                                          tgtgCTTAGAATGGAGCCGGAGACGCGCATTATGCTCTGGCAGGTTTGCCTAACACCTTTCTGGAACGCGAATATAAGATCCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCtat                                           <  1:3102049/150‑1 (MQ=255)
                                                                                                          tgtgCAGAGAAAGGCGCCGGAGACGCGCATTATGCTCTGGCTGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCtat                                           <  1:1726561/150‑1 (MQ=255)
                                                                                                           gtgCAGAGAAAGGAGCCGGAGATGCGCATTCGGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATACGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCtata                                          <  2:5958776/150‑1 (MQ=255)
                                                                                                           gtgCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCTAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCtata                                          <  2:5780981/150‑1 (MQ=255)
                                                                                                           gtgCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCtata                                          <  2:7198101/150‑1 (MQ=255)
                                                                                                              cAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCTGGTTTGCCTAACACCGTTCTGGCACGCTAATATAAGATGAAACTACCTGATGACAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGAc                                       <  1:3182443/150‑1 (MQ=255)
                                                                                                              cAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGAc                                       >  2:388238/1‑150 (MQ=255)
                                                                                                               agagAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACg                                      >  2:9027700/1‑150 (MQ=255)
                                                                                                               agagAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACg                                      >  1:9486321/1‑150 (MQ=255)
                                                                                                                tagaAGGGAGCCGGAGATGCGCATTAGGCTCGGGCTGGTTTGCATAAAACCTTTGTGTCAAGGGATTATAAGATGCAACTACCTGATGTTAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGc                                     <  1:9566247/149‑1 (MQ=255)
                                                                                                                gagaAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGc                                     >  2:2229995/1‑150 (MQ=255)
                                                                                                                         gCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAAttt                            <  2:5541559/150‑1 (MQ=255)
                                                                                                                          ccGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCta                                            >  1:9784829/1‑133 (MQ=255)
                                                                                                                          ccGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCACAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTc                           >  1:6927932/1‑150 (MQ=255)
                                                                                                                            ggAGAAGCGCTTTATGCTCTGGCAGTTTTGCCTAACACCGTTCTGGCACGCGAATATACGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAg                         <  2:4157942/150‑1 (MQ=255)
                                                                                                                            ggAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACAACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAg                         <  1:7164391/150‑1 (MQ=255)
                                                                                                                                 aGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCTAATATACGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGtt                    <  1:7453089/150‑1 (MQ=255)
                                                                                                                                  gcgcATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGttt                   >  1:5169096/1‑150 (MQ=255)
                                                                                                                                   cgcATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTc                  >  2:8817190/1‑150 (MQ=255)
                                                                                                                                       ttttGCTCTGGCTGGTTTGCCAAACACCTTTCTGGAAAGCTAATATAAGATGAAACTACCTTTTGATAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAAt              <  1:3836281/147‑1 (MQ=255)
                                                                                                                                        tATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAAtt             >  2:4820206/1‑150 (MQ=255)
                                                                                                                                          tGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATACGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCt           <  2:11097102/150‑1 (MQ=255)
                                                                                                                                           gCTCTGGCAGGTTTGCCTATCACCGTTCTGGCAAGCGATTTTAAGATGCAACTACCTGATTAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTg          <  2:8628328/150‑1 (MQ=255)
                                                                                                                                           gCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTg          <  2:2782779/150‑1 (MQ=255)
                                                                                                                                            ctctGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGc         >  2:7871050/1‑150 (MQ=255)
                                                                                                                                             tctGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAg                         >  1:182418/1‑133 (MQ=255)
                                                                                                                                             tctGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCa        <  2:1442644/150‑1 (MQ=255)
                                                                                                                                                 gCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACa    >  1:7550944/1‑150 (MQ=255)
                                                                                                                                                   aGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgt  >  2:2562635/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGT  >  NC_000913/3368687‑3368983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: