Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,532 C→A 8.2% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5320CA 8.2% 1260.7 / 21.4 440V69L (GTA→TTA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (241/146);  new base A (28/25);  total (269/171)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 440 total aligned reads displayed.

TATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAA  >  NC_000913/238384‑238679
                                                                                                                                                    |                                                                                                                                                   
tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                                    >  1:5716313/1‑150 (MQ=255)
     tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                               >  2:471512/1‑150 (MQ=255)
         aCAAAAGTAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTATGAATGATTTTATTATCGTAAGTAAATTTTCATTAATAttt                                                                                                                                           >  2:6794859/1‑150 (MQ=255)
         aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                           <  1:10001314/150‑1 (MQ=255)
         aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                           >  2:5777933/1‑150 (MQ=255)
         aCAAAAGGAGCTATGAAAGGTGCTATTACGAGAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                           <  1:6354985/150‑1 (MQ=255)
          cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                          >  1:8758145/1‑150 (MQ=255)
           aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                         <  1:8686045/150‑1 (MQ=255)
              aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                      <  1:5299287/150‑1 (MQ=255)
              aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                      <  2:7788274/150‑1 (MQ=255)
              aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                      <  1:2893939/150‑1 (MQ=255)
               ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                     >  2:437934/1‑150 (MQ=255)
                    tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                <  2:9788184/150‑1 (MQ=255)
                    tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTAACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                <  1:1358563/150‑1 (MQ=255)
                     aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                               <  2:2723201/150‑1 (MQ=255)
                      ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACg                                                                                                                              >  2:6397417/1‑150 (MQ=255)
                       tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                             <  2:6482005/150‑1 (MQ=255)
                       tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                             <  2:7949669/150‑1 (MQ=255)
                       tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                             <  2:8652809/150‑1 (MQ=255)
                        aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                            >  2:3887030/1‑150 (MQ=255)
                            gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                        >  2:2501551/1‑150 (MQ=255)
                                tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                                    >  1:8111853/1‑150 (MQ=255)
                                    aCGATAGATATTTGTAAAAATATAAGAGTTAGCTTTATTGTTATTTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                                                <  2:9095854/150‑1 (MQ=255)
                                       aTAGATTTTAGTAAAAATATAAGTGTTAGCTGTATTGTTATGTCTGTGGCGATATTGACTCCCTTCGTTTTTTTGATTATGATTGATTTTTTTATCTTATGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                             <  2:4636042/150‑1 (MQ=255)
                                       aTAGATATTATTAAAAATATAAGATTTAGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGcac                                                                                                             <  2:3040076/150‑1 (MQ=255)
                                           cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                         >  2:8961754/1‑150 (MQ=255)
                                           aaataactAAAAATATAATAGTTATATGTATTGTTATGTCTTTGGAGAAATTTACTACATTCGTTTTTTTGATTAAGAATTAATTTATTATCGTAAGTAAAATTACAATAATATATAAAAATGAAAACGACATGAAACCGAAGAACAGaa                                                                                                         >  2:6864159/8‑150 (MQ=255)
                                            tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTTAGTATGATTTTTTTATCGTTAGTTAAATTACATGTATATTTTAAAATGAAAACGTCATGAAACCGAAGCACAGAAt                                                                                                        >  1:3201651/1‑150 (MQ=255)
                                            tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                        >  2:3796661/1‑150 (MQ=255)
                                            tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                        >  1:10195040/1‑150 (MQ=255)
                                              ttAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCa                                                                                                      >  2:9252152/1‑150 (MQ=255)
                                                aGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTGAGTAAAATTACATGAGTATTTGAAAAGGAAAACGACATGAAACCGAAGCACAGAGTCGAc                                                                                                    >  2:7314306/1‑150 (MQ=255)
                                                aGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                    <  2:7156890/150‑1 (MQ=255)
                                                aGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCATTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                    <  2:10110819/150‑1 (MQ=255)
                                                 gTAAAAATATAAGAGTTAGCCGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                                   >  1:5628666/1‑150 (MQ=255)
                                                  tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                                  >  2:212508/1‑150 (MQ=255)
                                                  tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACaa                                                                                                  >  1:10881065/1‑149 (MQ=255)
                                                   aaaaaTATAAGATTTAGCTTTATTTTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                 <  2:10568740/150‑1 (MQ=255)
                                                    aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                >  2:8194815/1‑150 (MQ=255)
                                                    aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAAACAACAAtc                                                                                                >  1:4966250/1‑150 (MQ=255)
                                                     aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAATTTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAATTTACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                               <  2:5241141/150‑1 (MQ=255)
                                                      aaTATATGTTTTAGCTTTATTGTTTTTTCTGTGGCTAAATTTACTACATTCTTTTTTTTTATTATTATTTATTTTATTTTCTTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                              <  1:6539674/150‑1 (MQ=255)
                                                         ataAGAGTTATCTGTATTGTTATGTCTGTGGCGAAATTGACTCCCTTCGTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                           <  2:6675236/150‑1 (MQ=255)
                                                         ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                           <  2:1689229/150‑1 (MQ=255)
                                                          tcagagTTAGCTTTATTTTTATGTCTGTGGCGAATTTGACTACCTTCTTTTTTTTTATTAAGATTTATTTTATTATCTTAATTAAAATTACATGAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                          <  1:2596410/148‑1 (MQ=255)
                                                           actttttAGCTGTTTTGTTTGTTCTGTGGCTAAATTGACTACCTTTTTTTTTTTTATTAATATTTTTTTTATTATCGTAAGAAAAATTAATTTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                                         <  1:9410484/145‑1 (MQ=255)
                                                                ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAAACGAAGCACAGAATCAACAATATCCAAACATaaa                                                                                    >  1:7869451/1‑150 (MQ=255)
                                                                   gCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACCAAGCACAGAATAAACATTAACCAATAaaaaaaaa                                                                                 >  1:5600797/1‑148 (MQ=255)
                                                                      gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGTTTTTATTATCCTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAAAAACATTCTCCAAACaaaaaaaaatt                                                                              >  1:9868441/1‑145 (MQ=255)
                                                                         ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                                           >  2:6597065/1‑150 (MQ=255)
                                                                         ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                                           >  2:2812818/1‑150 (MQ=255)
                                                                         ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAAAATATACAAACATAAAATATTTACg                                                                           >  1:6152606/1‑150 (MQ=255)
                                                                           gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                         >  2:10785593/1‑150 (MQ=255)
                                                                            ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAAATATTTTATTATAGTAAGTAAAAATAAAATAAAATTTAAAAATGAAAAAGACAGGAAAACCAAGCACAGAATCAACATTAAACAAAAATAAAAAATTTACGTgg                                                                        >  1:1587190/1‑150 (MQ=255)
                                                                            ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                                                        >  2:2246080/1‑150 (MQ=255)
                                                                             tATGTCTGTGGCTAAATTTAATACCTTCTTTTTTTTTATTAATAATGATTTTATTATCTTAATAAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                       <  1:10643852/150‑1 (MQ=255)
                                                                             tATGTCGGTGTCTAAATTGACTCCCGTCGTTTTTTTGATTAAGAATGTTTTTATTATCGTATTTAAAATTACTTGAATATTTAAAAAGTAAAACGACATGAATCCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTGCGTGGa                                                                       <  2:10268099/150‑1 (MQ=255)
                                                                                 tCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAt                                                                   >  2:1001972/1‑150 (MQ=255)
                                                                                  cTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATGATTTTATTATAGTAAGTAAAAATAAAAGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACACAATAAAAAAAATCCAAACATAAAAAATTTACGGGGAGCAtt                                                                  >  1:2353035/1‑150 (MQ=255)
                                                                                    gtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                >  2:3986083/1‑150 (MQ=255)
                                                                                    gtgGCGAAATTGACTACCTTCGTGTTTTTGTTTTAGAAATATTTTATTATAGTTAGTTAAAATAAATGAATATTTAAAAAGGAAAACGACAAGAAAACCAAGCACAGAAAAAACAATAACAAATCAAAAAAAATTTAAGTGGAGCAtttt                                                                >  1:7621131/1‑150 (MQ=255)
                                                                                     tggataaaTTGACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                               <  1:4788031/145‑1 (MQ=255)
                                                                                       gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACATAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                             >  2:9415181/1‑150 (MQ=255)
                                                                                        cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                            <  2:5364427/150‑1 (MQ=255)
                                                                                         gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                           >  2:1556885/1‑150 (MQ=255)
                                                                                          aaaTTGACCACCGTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                          <  2:9510711/150‑1 (MQ=255)
                                                                                             ttGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTAGCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                       <  1:3284696/150‑1 (MQ=255)
                                                                                                  tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTACAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                                  >  2:2363903/1‑150 (MQ=255)
                                                                                                      ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                              <  2:5628666/150‑1 (MQ=255)
                                                                                                       tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                                             >  2:7727879/1‑150 (MQ=255)
                                                                                                          ttttttttATTAAGAATGATTTTATTATCGTATGTAAATTTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                          <  1:3701078/150‑1 (MQ=255)
                                                                                                          tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                          >  1:6658999/1‑150 (MQ=255)
                                                                                                              tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                      >  2:2972414/1‑150 (MQ=255)
                                                                                                              tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                      >  2:1823399/1‑150 (MQ=255)
                                                                                                               ttGATTAATAATGTTTTTATTATCGTAATTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                     <  2:8924060/150‑1 (MQ=255)
                                                                                                               ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                     >  1:3946203/1‑150 (MQ=255)
                                                                                                               ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                     >  2:10970714/1‑150 (MQ=255)
                                                                                                                  aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                  >  1:1858879/1‑150 (MQ=255)
                                                                                                                  aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                  >  2:6062020/1‑150 (MQ=255)
                                                                                                                    tAAGATTGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                <  1:4858596/150‑1 (MQ=255)
                                                                                                                      aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                              >  2:7606324/1‑150 (MQ=255)
                                                                                                                             ttttATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                       <  1:7516363/150‑1 (MQ=255)
                                                                                                                             ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                       <  1:7192481/150‑1 (MQ=255)
                                                                                                                              tttATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                      <  1:4514896/150‑1 (MQ=255)
                                                                                                                              tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                      <  1:5936084/150‑1 (MQ=255)
                                                                                                                              tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                      >  1:7101696/1‑150 (MQ=255)
                                                                                                                              tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                      >  2:3143031/1‑150 (MQ=255)
                                                                                                                                  ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                  <  2:7247972/150‑1 (MQ=255)
                                                                                                                                     tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTGCGTGGAGGATTTTATTATTGAATATAGAGGTTTATCTCGGGTGAATATCTTTGAAGCATTGa               >  1:7328825/1‑150 (MQ=255)
                                                                                                                                      cttaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa              <  2:1121779/148‑1 (MQ=255)
                                                                                                                                       gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt             >  1:3535402/1‑150 (MQ=255)
                                                                                                                                       gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt             >  1:4153858/1‑150 (MQ=255)
                                                                                                                                          attaaAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa          <  2:5977958/148‑1 (MQ=255)
                                                                                                                                          agtaaAATTACATTAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa          <  2:337382/150‑1 (MQ=255)
                                                                                                                                            taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg        >  2:22074/1‑150 (MQ=255)
                                                                                                                                                aTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaa    >  1:8511714/1‑150 (MQ=255)
                                                                                                                                                aTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaa    >  2:3604555/1‑150 (MQ=255)
                                                                                                                                                aTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaa    >  1:1035667/1‑150 (MQ=255)
                                                                                                                                                aTTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaa    >  2:4198904/1‑150 (MQ=255)
                                                                                                                                                 ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa   >  2:10505712/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAA  >  NC_000913/238384‑238679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: