Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,439,588 G→C 5.1% intergenic (‑79/+28) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,5880GC 5.1% 2568.2 / 97.7 703intergenic (‑79/+28)yhdN/rplQDUF1992 family protein/50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (333/334);  new base C (16/20);  total (349/354)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 758 total aligned reads displayed.

AGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCG  >  NC_000913/3439439‑3439737
                                                                                                                                                     |                                                                                                                                                     
  gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCggg                                                                                                                                                     <  2:3133687/150‑1 (MQ=255)
  gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCggg                                                                                                                                                     >  2:8238950/1‑150 (MQ=255)
  gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCggg                                                                                                                                                     >  2:8636483/1‑150 (MQ=255)
  gTTATCAAACTCACCTTTCGCTTGCGCGTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCggg                                                                                                                                                     >  1:8460728/1‑150 (MQ=255)
   ttATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCgggg                                                                                                                                                    >  2:9625624/1‑150 (MQ=255)
    tATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGc                                                                                                                                                   >  2:4005363/1‑150 (MQ=255)
     aTCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAGGAGTCCAGCTATTGGAGATAAGTGGGGATACTCCGGGTATAAAAAAACCCGCCGGGGCg                                                                                                                                                  >  2:9451541/1‑150 (MQ=255)
       cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCggg                                                                                                                                                >  2:10004900/1‑150 (MQ=255)
          ccTCACCTTTCGCTTGCGCTTCGGCTATATGGCGCTCGGCCCACTGGTCAAGTAACCACATAAATACTCCCGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGGATAAAAAAACCCGCCCCGGCGGGttt                                                                                                                                             <  2:1858026/149‑1 (MQ=255)
           cTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCGGCCCACTGGCCAACTAACCACATAAATACTCCAGGGTATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttt                                                                                                                                            <  1:7052517/150‑1 (MQ=255)
             ccccTTGCGCGTGCGCTTCTGCTATCTGGCGCCCTGCCCACTGGTCAAGAAACCACATAACAACTCCAGGGGATTAACAAAAACCGTACAGATTTTGTATATATGTGGGGATACTCCGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                          <  1:353635/148‑1 (MQ=255)
             cACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                          <  2:7250253/150‑1 (MQ=255)
              aCCTTTCTCTTGCGCTTCTGATATTTGGCGCTCTGCCCACTGGTCAATTACCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATACGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGtttttttt                                                                                                                                         <  1:5415/150‑1 (MQ=255)
                   tCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGtt                                                                                                                                    >  2:6095848/1‑150 (MQ=255)
                      cTTGCGCTTCTGCTATAGGGCGCCCGGCCCACTGGACAAGCAACAACATAAATACGCCAGGGGATTAAAAAAAAGATTACAGCTTTTGTAGATAAGTGGGGAAACTACGGTTAAAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCt                                                                                                                                 <  1:2963903/150‑1 (MQ=255)
                        tGCGCTTGTGCTATTGGGCGCTCTGCCCACTGGTCAAGTACCCACATACATACGCCGGGGGATGAACAAAAAGTGTACCGCTTTGGTAGATACGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTc                                                                                                                               <  1:10653258/150‑1 (MQ=255)
                           gcTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGACCAAAAAGAGTACAGCTATTGTAGAGAAGTGGGGAGACTCCGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGa                                                                                                                            >  1:7668536/1‑150 (MQ=255)
                           gcTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGa                                                                                                                            <  2:3010321/150‑1 (MQ=255)
                           gcTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGa                                                                                                                            <  2:5336900/150‑1 (MQ=255)
                           gcTTCTGCTATATGGCGCTCGGCCCACTGGTCAAGTAACCACATAAATCCTCCCGGGGATGAACAAAATGAGTACAGCTTTTTTAGATATGTGGGTATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGa                                                                                                                            <  1:5228774/150‑1 (MQ=255)
                            cTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGAt                                                                                                                           >  1:5590614/1‑150 (MQ=255)
                             ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGAtt                                                                                                                          >  1:9845692/1‑150 (MQ=255)
                             ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGAtt                                                                                                                          >  2:8502835/1‑150 (MQ=255)
                             ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGAtt                                                                                                                          >  1:7775475/1‑150 (MQ=255)
                               cTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATCCACCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAc                                                                                                                        <  1:3391186/150‑1 (MQ=255)
                                  cTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActct                                                                                                                     >  1:8180495/1‑150 (MQ=255)
                                   tataTGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTg                                                                                                                    >  2:10830944/1‑150 (MQ=255)
                                     tatGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgca                                                                                                                  >  2:7810183/1‑150 (MQ=255)
                                     tatGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgca                                                                                                                  >  1:2465527/1‑150 (MQ=255)
                                      atGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAATAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcag                                                                                                                 >  1:6144877/1‑150 (MQ=255)
                                         gcgcTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACACTGcagcag                                                                                                              >  1:6733730/1‑150 (MQ=255)
                                           gcTCGGCCCACTGGACAAGTAACCACAAAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCt                                                                                                            <  1:4784806/150‑1 (MQ=255)
                                             tctGCCCACTGGTCAAGTAACCACATAACTACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTc                                                                                                          <  2:7125642/150‑1 (MQ=255)
                                              ctGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATCCTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCGTCAGATTACTCTGCAGCAGCTTCt                                                                                                         >  1:3835132/1‑150 (MQ=255)
                                               tGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTg                                                                                                        <  2:2432253/150‑1 (MQ=255)
                                               tGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTg                                                                                                        <  2:5559548/150‑1 (MQ=255)
                                               ggCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTCCCGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTg                                                                                                        <  1:9158529/149‑1 (MQ=255)
                                                  ccACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCtt                                                                                                     >  2:8386052/1‑150 (MQ=255)
                                                   cACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCttt                                                                                                    >  1:411609/1‑150 (MQ=255)
                                                    aCTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtc                                                                                                   >  2:8879398/1‑150 (MQ=255)
                                                    aCTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtc                                                                                                   >  1:4689944/1‑150 (MQ=255)
                                                     cTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtct                                                                                                  <  1:6328083/150‑1 (MQ=255)
                                                          cAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                                                                             <  2:1640288/150‑1 (MQ=255)
                                                              tAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGAt                                                                                         <  2:10677203/150‑1 (MQ=255)
                                                               aaCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATc                                                                                        <  2:10477721/150‑1 (MQ=255)
                                                               aaCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATc                                                                                        <  1:2280232/150‑1 (MQ=255)
                                                                  cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                     >  2:10328371/1‑150 (MQ=255)
                                                                  cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                     <  1:10843812/150‑1 (MQ=255)
                                                                  cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                     >  2:2255544/1‑150 (MQ=255)
                                                                  cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                     >  1:1152789/1‑150 (MQ=255)
                                                                  cacaGAAAGACTCCAGGGGATGAACAAAAAGCGGACCGCTTTTGTAGATACGTGGGGAGACTACGGGTATAAACAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                     <  2:7704563/150‑1 (MQ=255)
                                                                    caTCAATACTCCCGGGGATGAACAAAAAGAGTACCGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa                                                                                   <  2:9024243/150‑1 (MQ=255)
                                                                     aTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGACCGATCAACCAg                                                                                  >  1:4901136/1‑150 (MQ=255)
                                                                             tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                                          >  1:9461251/1‑150 (MQ=255)
                                                                             tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                                          >  1:7986941/1‑150 (MQ=255)
                                                                                    gATGAACAAAAAGTGTAGAGCTATTGTAGTTAAGTGGGGATACTACGGGTATAATTAATCCCGCCGGGGGGGGTTTTTTTTCGTTGGTTGTGTTTACTGTGCAGCTGGTTGTGGTTTGTCTGTAGGATGATCCAGGTCGTTGTAAgggat                                                                   >  2:4740187/1‑146 (MQ=255)
                                                                                    gATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCAt                                                                   <  1:8303963/150‑1 (MQ=255)
                                                                                      tGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCg                                                                 >  2:9207140/1‑150 (MQ=255)
                                                                                      tGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGATTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCg                                                                 <  1:10005238/150‑1 (MQ=255)
                                                                                       gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGCTGTAAGCCATCgg                                                                >  2:446301/1‑150 (MQ=255)
                                                                                       gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCAt                                                                   <  2:2004168/147‑1 (MQ=255)
                                                                                       gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCGTCTGCTTTCTCTGCACGATCAACCCGCTCGAGGTAAGCCATCgg                                                                >  2:10094640/1‑150 (MQ=255)
                                                                                         aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                              >  1:287226/1‑150 (MQ=255)
                                                                                         aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                              >  2:589144/1‑150 (MQ=255)
                                                                                         aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                              >  2:4228844/1‑150 (MQ=255)
                                                                                          cAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcgc                                                             <  2:1250862/150‑1 (MQ=255)
                                                                                          cAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcgc                                                             <  1:2741186/150‑1 (MQ=255)
                                                                                            aaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGt                                                           <  2:8107374/150‑1 (MQ=255)
                                                                                            aaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGt                                                           >  1:4459681/1‑150 (MQ=255)
                                                                                            aaaaGAGTACAGCTATTGTACATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTATACGTAGCATCAGATTACTCTGCAGCAGCTTCTGCTTTCTCAGAACGATCAACCAGCTCGCTGTAAGCCAGCGGCGCGt                                                           >  2:7491762/1‑150 (MQ=255)
                                                                                                 agTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCg                                                      <  2:10175568/150‑1 (MQ=255)
                                                                                                  gTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGc                                                     <  1:5082998/150‑1 (MQ=255)
                                                                                                  gTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGc                                                     <  2:10948089/150‑1 (MQ=255)
                                                                                                   tACAGCTATTGTATATAAGTGGTGTTACTACGTGTATAAAAAAACCCGCCGGGGCGGTTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGcc                                                    <  1:8684134/150‑1 (MQ=255)
                                                                                                   tACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGcc                                                    <  1:1418910/150‑1 (MQ=255)
                                                                                                   tACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGcc                                                    >  1:9722607/1‑150 (MQ=255)
                                                                                                       gCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCa                                                <  2:6824746/150‑1 (MQ=255)
                                                                                                       gCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCa                                                <  2:6914158/150‑1 (MQ=255)
                                                                                                           ttGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGa                                            >  2:3769005/1‑150 (MQ=255)
                                                                                                            tGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGaa                                           >  2:2677505/1‑150 (MQ=255)
                                                                                                               aGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcc                                        <  2:4985677/150‑1 (MQ=255)
                                                                                                                 aTAAGTGGGGATACTACGGGTATAATAATTCCCGCCGGGGCGGGTTTTTTTACGTTGGTTCAGATTACTCTGCAGCAGCTTCTGCTTTGTGTGAACGTTGAAGCAGCTCGATGTTAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcac                                      >  1:210489/1‑150 (MQ=255)
                                                                                                                    aGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACt                                   >  1:10119227/1‑150 (MQ=255)
                                                                                                                        gggATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCATATGCTTTCTCTGAACGATCAAACAGCTCGATGTAAGCCATCGGCGCGTTGTTGCCTGCACGGAAGCCACACTTCAg                               >  1:6440549/1‑150 (MQ=255)
                                                                                                                               tacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGa                        >  1:6083636/1‑150 (MQ=255)
                                                                                                                                   ggTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgtgt                    >  1:8808509/1‑150 (MQ=255)
                                                                                                                                       caaaaaaaCCCGCCGGGGCGGGTATTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCAACGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacc                <  1:9565675/149‑1 (MQ=255)
                                                                                                                                         aaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac              >  2:2859142/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac              <  1:10470667/150‑1 (MQ=255)
                                                                                                                                          aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccacc             >  1:7304884/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCGTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccacc             >  1:1851879/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGAGTACTCTGCAGCAGCGTCTGCTTTCTCTGAACGATCAACCAGCTCGAGGTAAGCCAGCGGCGCGGGGTCGCCTGCACGGACGCCACACGGCAGAATCCGAGTGTAcccccc             >  2:6429829/1‑150 (MQ=255)
                                                                                                                                           aaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCg            >  2:3452413/1‑150 (MQ=255)
                                                                                                                                           aaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTAAGAAAACACTGCAGCAGCGTCTGCTTTATCTGAACGATCAACCAGATCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCg            >  2:6641525/1‑150 (MQ=255)
                                                                                                                                              aCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCa         <  1:6941825/150‑1 (MQ=255)
                                                                                                                                              aCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCa         >  2:5380363/1‑150 (MQ=255)
                                                                                                                                                ccgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACg       >  2:2579868/1‑150 (MQ=255)
                                                                                                                                                 cgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACgg      <  1:9255495/150‑1 (MQ=255)
                                                                                                                                                   ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCt    <  2:1152789/150‑1 (MQ=255)
                                                                                                                                                    cgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTc   <  1:9022559/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCG  >  NC_000913/3439439‑3439737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: