Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,537,327 G→T 7.1% intergenic (+39/‑58) greB → / → yhgF transcript cleavage factor/putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,3270GT 7.1% 1927.0 / 16.0 582intergenic (+39/‑58)greB/yhgFtranscript cleavage factor/putative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base G (209/310);  new base T (61/2);  total (270/312)
Fisher's exact test for biased strand distribution p-value = 5.38e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 623 total aligned reads displayed.

CCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGG  >  NC_000913/3537178‑3537476
                                                                                                                                                     |                                                                                                                                                     
ccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTg                                                                                                                                                       >  1:1858024/1‑150 (MQ=255)
 cGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgc                                                                                                                                                      <  2:4145751/150‑1 (MQ=255)
 cGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgc                                                                                                                                                      >  2:3110363/1‑150 (MQ=255)
   aTGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccc                                                                                                                                                    >  2:4394380/1‑149 (MQ=255)
       cccGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTATGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccagcca                                                                                                                                                <  1:2194973/150‑1 (MQ=255)
       cccGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccagcca                                                                                                                                                <  1:1137855/150‑1 (MQ=255)
          gcgcAGTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTc                                                                                                                                             <  2:11097993/150‑1 (MQ=255)
           cgcATTGCTTAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTcc                                                                                                                                            <  1:5591685/150‑1 (MQ=255)
           cgcATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTcc                                                                                                                                            <  2:1057806/150‑1 (MQ=255)
               ttGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGtat                                                                                                                                        <  1:1218939/150‑1 (MQ=255)
                   tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACt                                                                                                                                    >  2:9719036/1‑150 (MQ=255)
                   tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACt                                                                                                                                    >  2:5632046/1‑150 (MQ=255)
                   tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCACTCCGTATAACt                                                                                                                                    >  1:2405232/1‑150 (MQ=255)
                   tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAATTTTTCCAGCCACTCCGGATaaaa                                                                                                                                    >  2:10327433/1‑148 (MQ=255)
                        aaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATccc                                                                                                                               <  1:5164975/150‑1 (MQ=255)
                         aagaagTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATcccc                                                                                                                              <  2:3361349/150‑1 (MQ=255)
                            aagTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGa                                                                                                                           >  2:6129669/1‑150 (MQ=255)
                             agTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAt                                                                                                                          <  1:8682923/150‑1 (MQ=255)
                              gTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAtt                                                                                                                         <  1:4072226/150‑1 (MQ=255)
                               tCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttt                                                                                                                        >  2:6788091/1‑150 (MQ=255)
                               tCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttt                                                                                                                        >  1:1569471/1‑150 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGGTGGCATTTTTTCCCGCCCCTCCGGATAAAAAACCCCGGAttttt                                                                                                                      >  1:2978127/1‑150 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttttt                                                                                                                      >  2:5408779/1‑150 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGAGGGCGGGGATTTTTGCCAGCCAGTCCGGATAACTATCCCCGGAgtttt                                                                                                                      >  2:12759/1‑150 (MQ=255)
                                     aTCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATc                                                                                                                  >  2:1823321/1‑150 (MQ=255)
                                     aTCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCACTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATc                                                                                                                  <  2:8929075/150‑1 (MQ=255)
                                      tCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCAGATTTTTGATcc                                                                                                                 >  1:7173555/1‑150 (MQ=255)
                                      tCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAAATATACCCGGATTTTTGTTcc                                                                                                                 >  2:3987107/1‑150 (MQ=255)
                                       cTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGGATTTTTTGCAGCCAGTCCGTATAAATATACCCTGATTTTTTAtagg                                                                                                                >  1:809254/1‑147 (MQ=255)
                                         ggCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGaa                                                                                                              <  2:8381633/150‑1 (MQ=255)
                                            ggtggtGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCCGCCAGTCCGTATAACTATCCCCAGATTTTTTTTCCGAAAAg                                                                                                           >  1:9015421/1‑150 (MQ=255)
                                                       ccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCCGTCCGTATAACTATACCCTGATTTTTGGTCCGAAAAGATGAACTCaaa                                                                                                >  1:2651990/1‑150 (MQ=255)
                                                        ccccGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAAc                                                                                               >  2:7244556/1‑150 (MQ=255)
                                                        cccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCCGTCCGTATAACTATACCCTGATTTTTGATCCGAAAAGATGAACTCAAAc                                                                                               >  1:6023107/1‑150 (MQ=255)
                                                           tGCCGGGGAAGCGAGCTGGTATGTTAATACTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCACTCCCTATAACAATACCCAGATTTTTGATCCGAAAAGATGAACACAAAACat                                                                                            >  2:369876/1‑150 (MQ=255)
                                                             ccGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCatga                                                                                          <  2:10140287/150‑1 (MQ=255)
                                                               ggggAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCatgatg                                                                                        >  2:6362125/1‑150 (MQ=255)
                                                                gggAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGGTGGCATTTTTTGCAGCCCCACCCTATAAAAATCCCCAGTATTTTGATACGAAAAGAAGAACAAAAAACAagatga                                                                                       >  1:1759476/1‑150 (MQ=255)
                                                                gggAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCCGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAtgatga                                                                                       >  2:10823152/1‑150 (MQ=255)
                                                                    aGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGatgaatga                                                                                   <  2:2060729/150‑1 (MQ=255)
                                                                           tGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTc                                                                            <  1:9611551/150‑1 (MQ=255)
                                                                           tGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCGGGGCTTTTTTCCAGCCCCTCCCGATAACAAAACCCAGATTTTTTTTCCGAAAAGATGAACTCAAACCAAGATGAAAGATTCGTTc                                                                            >  1:6560480/1‑150 (MQ=255)
                                                                            ggTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGGTGGGAATTTTTGCAGCCAGTCCCTATAAAAAAACCCAGATTTTTTTTGCGAAAAGATGTAAAAAAAACATGATGAAATATTAGttgg                                                                           >  1:8427715/1‑148 (MQ=255)
                                                                            ggTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCt                                                                           <  1:4518476/150‑1 (MQ=255)
                                                                             gTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACACAAACCATGATGAATGATTCGTTCTg                                                                          >  2:7122560/1‑150 (MQ=255)
                                                                             gTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTg                                                                          <  1:327265/150‑1 (MQ=255)
                                                                                 gTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTATTGCCAGCCAGTCCGTCTAACAAACCCCAGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGc                                                                      >  2:7784652/1‑150 (MQ=255)
                                                                                 gTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAATTTTTCCAGCCAGTCCGTATAACTAAACCCAGATTTTTGATCCGAAAAGATGAACTCAAACCAAGATGAATGATTAGGTCTGCCGc                                                                      >  2:487532/1‑150 (MQ=255)
                                                                                 gTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGAGGGGTGGGATTTTTTGCAGCCACTCCGGATAACTATCCCCAGAATTTTTTTGCGACAAGATTTAAACAAACCAAGATGAATTATTGGttgttgcgc                                                                      >  1:2010598/1‑143 (MQ=255)
                                                                                  ttAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGGATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAAAGATTAGTTCTGCCGCa                                                                     >  2:6693588/1‑150 (MQ=255)
                                                                                     aTGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGCACGCAAACCATGATGAATGATTCGTTCTGCCGCattc                                                                  >  2:10039379/1‑149 (MQ=255)
                                                                                         tATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGGCAGCCAGTGCGTTTAACTATCCCCTGATTTTTGATGCGTAAAGTTGATATCAAACCATGATGAATGTTTAGTTGTGGCGCATTATTgg                                                              >  2:3441211/1‑149 (MQ=255)
                                                                                         tATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGc                                                              <  2:9071955/150‑1 (MQ=255)
                                                                                          aTCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCg                                                             <  1:2729023/150‑1 (MQ=255)
                                                                                          aTCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCg                                                             <  1:4914756/150‑1 (MQ=255)
                                                                                          aTCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCg                                                             <  1:5363572/150‑1 (MQ=255)
                                                                                          aTCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCgg                                                            <  1:10147401/150‑1 (MQ=255)
                                                                                            cGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCggg                                                           <  1:5415924/150‑1 (MQ=255)
                                                                                             gAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGt                                                          <  2:6562118/150‑1 (MQ=255)
                                                                                               gTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACAATCCCCAGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCAATATTGCGGGTGa                                                        >  2:4983689/1‑150 (MQ=255)
                                                                                                tACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAAATATACCCGGATTTTTGATCCGAAAATTTTAACTCAAACCATGATTAATGATTCGTTCTGCCGCAATATTGCGGGTGaa                                                       >  2:8536449/1‑150 (MQ=255)
                                                                                                 aCGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGaaa                                                      <  1:585031/150‑1 (MQ=255)
                                                                                                 aCGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGaaa                                                      <  2:4732691/150‑1 (MQ=255)
                                                                                                  cGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCACCCGCAATAATGCggcagaacg                                                     <  2:8007352/150‑8 (MQ=255)
                                                                                                   gTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAAtt                                                    >  2:1496493/1‑150 (MQ=255)
                                                                                                   gTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCAGATTTTTGATCCGAAAAGATTAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAAtt                                                    >  2:10402201/1‑150 (MQ=255)
                                                                                                    tGAAACCGTAAGGAAGAGTCTTAACGTCCTGCCGATGGCTGGGATTTTTGCCAGGCAGTCCGTATTAGTATCCCCTGTTTTTTTATGCGTATAGTTGTAGTCAAACCATGTTGTTTTTTTGGTTGTGGCGGATTATTGGGGGTGTAAttg                                                   >  2:933237/1‑149 (MQ=255)
                                                                                                        aCCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGCCATTATTGCCAGCCAGTCAGTATAACTATCCCCTGATTATTGATCCGAAAAGATGAACTCAAACCATGATGAATGATACGTTCTGCCGCATTATTGCGGGTGAAATTCAGgc                                               <  1:7678995/150‑1 (MQ=255)
                                                                                                          cGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGGTGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGCAATTCAGgcgc                                             >  2:5796425/1‑150 (MQ=255)
                                                                                                                  agagTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGaa                                     >  2:751517/1‑150 (MQ=255)
                                                                                                                  agagTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGaa                                     <  1:3799396/150‑1 (MQ=255)
                                                                                                                     gTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAg                                  <  1:10044310/150‑1 (MQ=255)
                                                                                                                      tCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAgg                                 <  1:7056598/150‑1 (MQ=255)
                                                                                                                           aCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGAc                            <  1:4784696/150‑1 (MQ=255)
                                                                                                                            cctcctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACg                           <  1:3483281/150‑1 (MQ=255)
                                                                                                                               cctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTg                        <  2:9456671/150‑1 (MQ=255)
                                                                                                                                ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGc                       <  2:517065/150‑1 (MQ=255)
                                                                                                                                ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGc                       >  2:9202140/1‑150 (MQ=255)
                                                                                                                                ctGCCGATGGCTGGCATTTTTGCCAGCCAGTACGTATAACTATCCCCAGATGTTTGATCCGAAAAGATGAACGCAAACCATGATGAATGAGTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGc                       >  2:8787081/1‑150 (MQ=255)
                                                                                                                                ctGCCGATGGCTGGCAATTTTTCCAGCCAGTCCGTATAACTATCCCCTGATTTTTTATACGAAAATATGAACTCAAAACATGATTAAATATTCGTTCTGCCGCATTATTGCGGGTGAAAATCAGGCGCGCCCGGAACAGGTTGACGCTGc                       >  2:2976498/1‑150 (MQ=255)
                                                                                                                                 tggcGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGcc                      <  2:5982951/147‑1 (MQ=255)
                                                                                                                                 tGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAAGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGcc                      >  2:6974354/1‑150 (MQ=255)
                                                                                                                                     gATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTc                  >  1:9356086/1‑150 (MQ=255)
                                                                                                                                     gATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTc                  <  1:4393907/150‑1 (MQ=255)
                                                                                                                                       tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGCTGAACTCAAACCATGATGACTGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACCGGTTGACGCTGCCGTTCGc                <  2:7357403/150‑1 (MQ=255)
                                                                                                                                        ggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGcc               <  1:10865445/150‑1 (MQ=255)
                                                                                                                                         gctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCt              >  1:6892528/1‑150 (MQ=255)
                                                                                                                                         gctggcATGTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCt              <  2:2548161/150‑1 (MQ=255)
                                                                                                                                          ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTg             >  1:9555248/1‑150 (MQ=255)
                                                                                                                                          ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTAGTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTg             <  1:7388768/150‑1 (MQ=255)
                                                                                                                                           tgtcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            <  1:1031392/147‑1 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            >  1:8287398/1‑150 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            >  1:4321635/1‑150 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            >  1:6353253/1‑150 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            <  1:9811194/150‑1 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            <  1:6169031/150‑1 (MQ=255)
                                                                                                                                             gcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGAGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCtt          >  1:10559790/1‑150 (MQ=255)
                                                                                                                                                tttttGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGAc       >  2:8157887/1‑150 (MQ=255)
                                                                                                                                                  tttGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGa     >  2:4235989/1‑150 (MQ=255)
                                                                                                                                                   ttGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGaa    <  2:2388463/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGG  >  NC_000913/3537178‑3537476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: