Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,535 G→T 5.6% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5350GT 5.6% 1317.6 / 6.6 447H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (265/136);  new base T (11/34);  total (276/171)
Fisher's exact test for biased strand distribution p-value = 1.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 457 total aligned reads displayed.

CCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238387‑238684
                                                                                                                                                    |                                                                                                                                                     
 cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTTAGTAAAATTAAATGaa                                                                                                                                                     >  1:3230690/1‑150 (MQ=255)
      aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                                <  1:10001314/150‑1 (MQ=255)
      aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                                >  1:3714785/1‑150 (MQ=255)
        aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                              >  2:7168640/1‑150 (MQ=255)
         aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                             <  1:4697863/150‑1 (MQ=255)
         aaaGGAGCTATTAAAGGTGCTATTAAGATACCAATTAGTAAAAATATAAGAGATAGCTGTATTGATATGTCTGTGGCGAAATTGACTACCTACCTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                             <  2:897292/150‑1 (MQ=255)
          aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaa                                                                                                                                            <  2:11052032/150‑1 (MQ=255)
           aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:7196253/150‑1 (MQ=255)
           aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:2893939/150‑1 (MQ=255)
            ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                          >  2:7479468/1‑150 (MQ=255)
                 tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     <  2:9788184/150‑1 (MQ=255)
                 tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTAACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     <  1:1358563/150‑1 (MQ=255)
                  aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                                    <  2:2723201/150‑1 (MQ=255)
                        ggTGCTATTACTATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                              <  2:6819744/150‑1 (MQ=255)
                        ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                              >  1:4933670/1‑150 (MQ=255)
                         gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                             <  2:5310148/150‑1 (MQ=255)
                         gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                             >  2:2501551/1‑150 (MQ=255)
                               ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                                                       >  2:3929070/1‑150 (MQ=255)
                                tACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAAACGaa                                                                                                                      >  2:7840788/1‑150 (MQ=255)
                                 aCGATAGATATTTGTAAAAATATAAGAGTTAGCTTTATTGTTATTTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                                                     <  2:9095854/150‑1 (MQ=255)
                                    aTAGCTTTTAGTAAAAATATAATAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                                  <  2:2337148/150‑1 (MQ=255)
                                    aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGcac                                                                                                                  >  1:8181885/1‑150 (MQ=255)
                                    aTAGATATTATTAAAAATATAAGATTTAGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGcac                                                                                                                  <  2:3040076/150‑1 (MQ=255)
                                         tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaaa                                                                                                             >  1:11038353/1‑149 (MQ=255)
                                         tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                             >  1:10195040/1‑150 (MQ=255)
                                           ttAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCa                                                                                                           >  2:9252152/1‑150 (MQ=255)
                                               tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                                       >  2:212508/1‑150 (MQ=255)
                                               tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAATAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACAt                                                                                                       >  1:5135160/1‑150 (MQ=255)
                                                aaaaaTATAAGATTTAGCTTTATTTTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                      <  2:10568740/150‑1 (MQ=255)
                                                 aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAAtc                                                                                                     >  1:3544662/1‑150 (MQ=255)
                                                  aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                                    >  1:638533/1‑150 (MQ=255)
                                                   aaTATATGTTTTAGGTTTTTTGTTTGGTCTGTGGCGATTTTCACTCCCTTGTTTTTTTTTATTAAGATTTTTTTTTTTTTCTTATGAAAAATTACTTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                   <  1:1913777/150‑1 (MQ=255)
                                                   aaTATATGTTTTAGCTTTATTGTTTTTTCTGTGGCTAAATTTACTACATTCTTTTTTTTTATTATTATTTATTTTATTTTCTTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                   <  1:6539674/150‑1 (MQ=255)
                                                      ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                                <  2:1689229/150‑1 (MQ=255)
                                                        actttttAGCTGTTTTGTTTGTTCTGTGGCTAAATTGACTACCTTTTTTTTTTTTATTAATATTTTTTTTATTATCGTAAGAAAAATTAATTTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                                              <  1:9410484/145‑1 (MQ=255)
                                                          gagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAt                                                                                            >  2:10468168/1‑150 (MQ=255)
                                                             ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAAACGAAGCACAGAATCAACAATATCCAAACATaaa                                                                                         >  1:7869451/1‑150 (MQ=255)
                                                                gCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCAAATCATAAAata                                                                                      >  2:5502086/1‑150 (MQ=255)
                                                                 cttttttGTTTTGTCTGTGGCTAAATTTACTACCTTCTTTTTTTTTATTAATAATTTTTTTATTATCTTATTTAAATTTACTTTATTATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                     <  1:5611872/145‑1 (MQ=255)
                                                                  tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                                    >  2:2235336/1‑150 (MQ=255)
                                                                    tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATTAAACCGAAGCACAGAATCAACATTATACAATCATAAAAAAttta                                                                                  >  1:7350716/1‑149 (MQ=255)
                                                                    tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                  >  2:8111227/1‑150 (MQ=255)
                                                                     aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTATAATTATTTTATTATAGTAAGTAAAAATAAAATAATAATTAAAAAGGGAAAACACATGTAAACCAAGCACAGAATAAAAAATATCCAAAAAAAAAATAtttac                                                                                 >  1:4848822/1‑148 (MQ=255)
                                                                      ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                                                >  2:2812818/1‑150 (MQ=255)
                                                                      ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                                                >  2:3613133/1‑150 (MQ=255)
                                                                      ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAAAATATACAAACATAAAATATTTACg                                                                                >  1:6152606/1‑150 (MQ=255)
                                                                       tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                               <  2:4468515/150‑1 (MQ=255)
                                                                        gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACAGAATAAACAATATCCAATAATAAAAAATTTACGTg                                                                              >  1:5707003/1‑150 (MQ=255)
                                                                        gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                              >  2:10785593/1‑150 (MQ=255)
                                                                         ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAAAGAATTTATTATAGTAAGTAAAAATAAAAGAAAATTTTAAAAGGAAAACGACAAGAAACCCAAGCAAACAAAAAACAAACTCCAATCATAAAAAATTTACGTgg                                                                             >  1:2612720/1‑150 (MQ=255)
                                                                          tATGTCTGTGGCTAAATTTAATACCTTCTTTTTTTTTATTAATAATGATTTTATTATCTTAATAAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                            <  1:10643852/150‑1 (MQ=255)
                                                                          tATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGTTTAAGATTTATTTTATTATCTTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                            <  1:9871739/150‑1 (MQ=255)
                                                                          tATGTCTGTGGCGAAATTGAATACATTCGTTTTTTTTATTAATAATTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                            <  2:2207233/150‑1 (MQ=255)
                                                                           aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATAGTAAGTAAAATTAAAAGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAAAATTATACAAAAAAAAAAAATTTACGTggtg                                                                           >  1:7654715/1‑148 (MQ=255)
                                                                            tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGc                                                                          >  2:6531853/1‑150 (MQ=255)
                                                                              tCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAATTAAAAATGAAAACGACATGAAAACGAAGCACAGAATCAAAATTATCCAATAATAAAATATTTCCGTGGAGCAt                                                                        >  2:10185181/1‑150 (MQ=255)
                                                                               cTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATGATTTTATTATAGTAAGTAAAAATAAAAGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACACAATAAAAAAAATCCAAACATAAAAAATTTACGGGGAGCAtt                                                                       >  1:2353035/1‑150 (MQ=255)
                                                                                tgtgGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATTAAAAGAATATTTAAAAATGAAAACGCAAAGAAACCCAAGCACAGAAAAAACAATAACCAATCAAAAAATATTTACGGGGTGCAttt                                                                      >  1:1625513/1‑150 (MQ=255)
                                                                                 gtgGCTAAATTGACTCCCTTCTTTTTTTTTTTAAATAATGATTTTTTTTTCTTAAGTAAAATTACATTATTTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                     <  1:3320662/150‑1 (MQ=255)
                                                                                  tgGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATTAAAAGGATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACAGAAAAAACAATATCCAAACAAAAAATATTTACGGTGAGGATTtta                                                                    >  1:7114620/1‑150 (MQ=255)
                                                                                    gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                                  >  2:9415217/1‑150 (MQ=255)
                                                                                    gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTttatt                                                                  >  2:6155160/1‑150 (MQ=255)
                                                                                      gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                                >  2:7732592/1‑150 (MQ=255)
                                                                                       aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:5716313/150‑1 (MQ=255)
                                                                                       aaaTTGACCACCGTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:9510711/150‑1 (MQ=255)
                                                                                          ttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                            <  1:5497347/150‑1 (MQ=255)
                                                                                                 ccTACGTTTATTTGATAAACAATCAATTTATTATCCTAAGAAAAATTACATGAATATAAAAAAAGGAAAACGACATGAAACCCAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                     <  2:1534335/150‑1 (MQ=255)
                                                                                                    tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                                                  >  2:4693316/1‑150 (MQ=255)
                                                                                                       ttttttttATTAAGAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                               <  2:10298492/150‑1 (MQ=255)
                                                                                                           tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACtac                                           >  2:4102722/1‑148 (MQ=255)
                                                                                                           tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           >  2:2972414/1‑150 (MQ=255)
                                                                                                            ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                          >  1:3946203/1‑150 (MQ=255)
                                                                                                            ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                          <  2:6454917/150‑1 (MQ=255)
                                                                                                            ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                          <  2:8924120/150‑1 (MQ=255)
                                                                                                                ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                      >  2:8678562/1‑150 (MQ=255)
                                                                                                                ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                      >  2:3197798/1‑150 (MQ=255)
                                                                                                                ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                      >  1:8689332/1‑150 (MQ=255)
                                                                                                                 tAAGAATGATTTTATTATCGTAAGTAAAATTACATGTATATTTAATAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGATTATAGAGGTTTATCTGCGGTaaa                                     >  2:1828953/1‑150 (MQ=255)
                                                                                                                 tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                     >  2:4261158/1‑150 (MQ=255)
                                                                                                                     aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAGAc                                 >  2:6792280/1‑150 (MQ=255)
                                                                                                                     aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                                 >  1:6707035/1‑150 (MQ=255)
                                                                                                                        taTTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  2:7678573/149‑1 (MQ=255)
                                                                                                                        gATTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  1:10432327/150‑1 (MQ=255)
                                                                                                                          ttttATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                            <  1:7516363/150‑1 (MQ=255)
                                                                                                                           tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           <  1:5936084/150‑1 (MQ=255)
                                                                                                                            ttattaTCTTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag                          <  2:4831598/150‑1 (MQ=255)
                                                                                                                               ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                       >  1:8515866/1‑150 (MQ=255)
                                                                                                                               ttatAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                       <  2:7248002/150‑1 (MQ=255)
                                                                                                                                tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                      >  2:5996307/1‑150 (MQ=255)
                                                                                                                                tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                      >  2:6604473/1‑150 (MQ=255)
                                                                                                                                  tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa                    >  1:8807094/1‑150 (MQ=255)
                                                                                                                                   cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                   >  2:10960648/1‑150 (MQ=255)
                                                                                                                                    gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                  >  1:4153858/1‑150 (MQ=255)
                                                                                                                                       agtaaAATTACATTAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa               <  2:337382/150‑1 (MQ=255)
                                                                                                                                       agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa               >  2:3801377/1‑150 (MQ=255)
                                                                                                                                                  aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa    >  2:6957960/1‑150 (MQ=255)
                                                                                                                                                    gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata  >  2:10051528/1‑150 (MQ=255)
                                                                                                                                                    gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata  >  1:4409692/1‑150 (MQ=255)
                                                                                                                                                    gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata  >  2:5904146/1‑150 (MQ=255)
                                                                                                                                                    gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAataata  >  2:8573942/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238387‑238684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: