Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,038 T→A 18.4% intergenic (+21/‑177) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0380TA18.4% 1284.5 / 61.4 515intergenic (+21/‑177)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (39/347);  new base A (70/2);  total (166/349)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 524 total aligned reads displayed.

ATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCT  >  NC_000913/3780889‑3781187
                                                                                                                                                     |                                                                                                                                                     
   aaaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAAAGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAc                                                                                                                                                    <  1:10008334/150‑1 (MQ=255)
     aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAacc                                                                                                                                                  >  1:6911647/1‑150 (MQ=255)
       tGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCctc                                                                                                                                                <  1:3081105/150‑1 (MQ=255)
         gCGATGACCCTGACTGCCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGAAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                                                              <  2:1349571/150‑1 (MQ=255)
           gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAACCCACTccc                                                                                                                                            >  1:698057/1‑150 (MQ=255)
            aTGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCt                                                                                                                                           <  2:10653777/150‑1 (MQ=255)
               aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                        <  2:1455668/150‑1 (MQ=255)
               aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                        <  2:2335111/150‑1 (MQ=255)
                cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                                       <  2:52833/150‑1 (MQ=255)
                cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                                       <  2:11033755/150‑1 (MQ=255)
                 gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCCAAACCACACCCACCggg                                                                                                                                      >  1:3208414/1‑150 (MQ=255)
                    gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                                                                   <  2:2894731/150‑1 (MQ=255)
                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                                                  <  1:207053/150‑1 (MQ=255)
                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                                                  <  1:3335780/150‑1 (MQ=255)
                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCACCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                                                  <  2:6075134/150‑1 (MQ=255)
                      cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                 <  2:866477/150‑1 (MQ=255)
                       tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                <  1:2284403/150‑1 (MQ=255)
                       tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                <  1:9397773/150‑1 (MQ=255)
                        ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCACTCCCGCAGGGGGAgggg                                                                                                                               >  1:6020310/1‑150 (MQ=255)
                         gcgcGAAATCGATCAGCGACATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                              <  1:2721311/150‑1 (MQ=255)
                         gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGGCCCCGCACCCAAACCCGCACCCACAGGGCGAGGGGa                                                                                                                              >  1:10878388/1‑150 (MQ=255)
                         gcgcGAAATCGATCAGCGAAAGTACGCAAGATTCGCTGGTGCAGGGGCTGGGTACAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                              <  1:9114787/150‑1 (MQ=255)
                            cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttagg                                                                                                                           <  2:6830583/150‑6 (MQ=255)
                                aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                       <  1:5799233/150‑1 (MQ=255)
                                 tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGACTGCGGCATAGTCGTGTGCGCCCCCGCCCCCAAAACCACACCCCCAGGGGGAGGGGGcccgtggg                                                                                                                      >  1:6581291/1‑144 (MQ=255)
                                 tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCCAACCCCCACCCTCAGGGAGGGGGGGCCGGTCgg                                                                                                                      >  1:1314329/1‑150 (MQ=255)
                                  cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                     <  1:8592398/150‑1 (MQ=255)
                                       aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACACCCACAGGGAGAGGGGACCGTTCGGCGCtgt                                                                                                                >  1:7139544/1‑150 (MQ=255)
                                       aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACAACCACAAGGGGAGGGGGCCCATCGgcgcggg                                                                                                                >  1:8718849/1‑147 (MQ=255)
                                       aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCCACCCACCCCCACCGGGCGGGGGGGCCCGGCGgcgccgg                                                                                                                >  2:4448208/1‑147 (MQ=255)
                                        gCGACATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgta                                                                                                               <  2:10733667/150‑1 (MQ=255)
                                        gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCACGACCACAGGGGGAGGGGGCCGTTAGGCGCtgga                                                                                                               >  2:118488/1‑148 (MQ=255)
                                        gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCACACCCACCGGGAGAGGGGGCCGGTCGGCGCggtt                                                                                                               >  1:534742/1‑149 (MQ=255)
                                         cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCACAACCACACGGCCAGGGGCCCCGTCGgcgcggaga                                                                                                              >  2:6442110/1‑145 (MQ=255)
                                          gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCACACGGAGAGGGGACCGTTCGGCGCggtatg                                                                                                             >  1:10139673/1‑150 (MQ=255)
                                             aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCCCAACCACAGGGGGAGGGGGCCCGTGGGCGCtggttgtta                                                                                                          >  1:4880314/1‑143 (MQ=255)
                                                 cGCAAGATTTGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCAAAACCACAACCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTAATccc                                                                                                      >  1:6854713/1‑150 (MQ=255)
                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACAACCAAACCCACAACCCCACGGCGAGGGGGCCCGGCGGCGCggtatgtaatacc                                                                                                      >  1:4296169/1‑145 (MQ=255)
                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                                      <  2:4528662/150‑1 (MQ=255)
                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCCCTACCGCAGGGGGAGGGGGCCCTTCGGCGCGGTATGTACAccc                                                                                                      >  2:3517595/1‑150 (MQ=255)
                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACACCCACAGGGAGAGGGGGCCGGTAGGCGCtgtatttaaaacc                                                                                                      >  1:4549070/1‑145 (MQ=255)
                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAAACCACAACCACCCGGGGCGGGGGCCCGTCGGCGCGGTATGTTCTacc                                                                                                      >  2:3331028/1‑150 (MQ=255)
                                                  gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAAACACAACCACACGGGGAGGGGGCCCGTCGGCGCAGTATGTACAcccc                                                                                                     >  1:9573010/1‑149 (MQ=255)
                                                   cAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGGGCCCCCCCCCCCCAACCCACCCCCACACGGGGAGGGGCCCGGTCGGGGCgggatgtaaaaaccc                                                                                                    >  1:9374218/1‑143 (MQ=255)
                                                       aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                                                <  1:5093677/150‑1 (MQ=255)
                                                        ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActct                                                                                               <  2:4906487/150‑1 (MQ=255)
                                                          cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                             <  2:10042830/150‑1 (MQ=255)
                                                          cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                             <  2:758480/150‑1 (MQ=255)
                                                           gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                                            <  1:948230/150‑1 (MQ=255)
                                                            cTGGTGCAGGGGCTGGGTAATGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                           <  1:1672484/150‑1 (MQ=255)
                                                            cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                           <  1:2518382/150‑1 (MQ=255)
                                                            cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                           <  2:4172766/150‑1 (MQ=255)
                                                             tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                          <  2:8745213/150‑1 (MQ=255)
                                                             tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                          <  2:7817129/150‑1 (MQ=255)
                                                                     gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCACACCCAAAACCACACCCACACGGGGAGGGGCCCCGTCGGCGCGGTATTTACACCCCCACACAGAAAAGACAcccc                                                                                  >  1:6430993/1‑148 (MQ=255)
                                                                     gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAAACCACACCCACACGGCGAGGGGTCCGGTCGGCGCGGTATGGAATCCCGCCCGCAGTAACGAAAcccc                                                                                  >  1:3649475/1‑148 (MQ=255)
                                                                           ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCCCCCCCCCACCCCAACCCCAACCCTCCCCGCGAGGGGCCCGGTCCGCGCGGGATTGACACCCCCACGCGGAAACGACACCCCACGCtt                                                                            >  2:3635122/1‑150 (MQ=255)
                                                                            gTAATGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                                           <  1:7739330/150‑1 (MQ=255)
                                                                             tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                          <  2:9414389/150‑1 (MQ=255)
                                                                                agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                       <  1:723919/150‑1 (MQ=255)
                                                                                agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCTCTCCCTCTGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTAACTCTGAAACGACACCGCACTCttttttt                                                                       <  1:1949299/150‑1 (MQ=255)
                                                                                agagTTGCCTGCGGCACGGGCTCCCTTGGCGATAGGGAATGCGGCATTGTCTTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTATGAAACGACACCGCACTCttttttt                                                                       <  1:1190198/150‑1 (MQ=255)
                                                                                    ttGCCTGCGGCACTTGCTCCCATGGCGAAAGGGAATGCGGCTTAGTCGTTTGCGCCCCCTCTCCCTTTCCCTCTCGCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGTTACGACACCGCACTCTTTTTTtctgc                                                                   <  2:7240167/150‑3 (MQ=255)
                                                                                      gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCt                                                                 <  1:6316795/150‑1 (MQ=255)
                                                                                       ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCCAAACCAAGCCCACCGGGAGAGGGGCCCCTTCGGCGCTGTATGTACTCCCACACACGGGAAAGACACCCCACTAATTTTTTATCCCTc                                                                >  2:4651791/1‑150 (MQ=255)
                                                                                        cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                               <  2:3849117/150‑1 (MQ=255)
                                                                                         tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                              <  2:9487070/150‑1 (MQ=255)
                                                                                         tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACTCCCTCAGGGGGGGGGGCCCCTTCGGCGCGGTTTGTAATACCGCCCACGGAAACCACACCGCCCACGTTTTTTATcccaccc                                                              >  2:6709063/1‑146 (MQ=255)
                                                                                           cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                            <  1:7982818/150‑1 (MQ=255)
                                                                                           cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCAGAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                            <  2:5820453/150‑1 (MQ=255)
                                                                                                 tGGCTCCCATGGCGAAAGGGAATGCGGCTTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                      <  1:7561538/150‑1 (MQ=255)
                                                                                                 tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                      <  2:1847957/150‑1 (MQ=255)
                                                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                     <  1:11128268/150‑1 (MQ=255)
                                                                                                   gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAAACCGCACCCACAGGGGGGGGGGGCCCGTCGGGGCGGGGTGTAATACCACACACTGTAACGACACCCCACCAATTTTTTATGCCACGCCCCGCCgggg                                                    >  1:10786163/1‑150 (MQ=255)
                                                                                                    cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                   <  2:7936470/150‑1 (MQ=255)
                                                                                                    cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                   <  1:1049976/150‑1 (MQ=255)
                                                                                                     tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCCCAACCCCACCCCCCGGGCGAGGGGCCCCGTCCGCGCGGGATTTAAACCCACAAAAAGAAACCACACCCCACACATTTTTTATACCACGCCCCCCCcgggcg                                                  >  2:2523704/1‑148 (MQ=255)
                                                                                                         aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCGAACCCACTACCTCAGGGCGAGGGGACCCTTCGGCGCTGTATGTACACCCTCACGCTGAAACCACACCGCCCACTTTTTTTATACCACGCCCCACCCGGGAGAggg                                              >  2:8232612/1‑150 (MQ=255)
                                                                                                         aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCGAAACCACAACCTCAGGGAGAGGGGGCCGGTCGGCGCAGTATTTACGCCCTCACTCTGTAACGACACCGCCCTCATTTTTTCTCCCTCGCCCCACCGGGgaggtgg                                              >  2:9225680/1‑146 (MQ=255)
                                                                                                           tgCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGcc                                            <  1:890247/149‑1 (MQ=255)
                                                                                                           ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCTCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGcc                                            <  1:4645460/150‑1 (MQ=255)
                                                                                                             cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                          <  1:6156948/150‑1 (MQ=255)
                                                                                                              gAACGGGAATGCGGCAGAGTCGTTTGCGCCCCCTCCCCCTACCCCGCGCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTAGGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                         <  2:1568045/150‑1 (MQ=255)
                                                                                                              gAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAACCCAATCCCACACGGAGCGGGGACCGGTCGGCGCGGTGTGTACACCCCCCCACAGAAACCACCCCCCCCACATTTTTTATCCCACCCCCCCCCCGGGCgcggggcggg                                         >  2:2444325/1‑150 (MQ=255)
                                                                                                                   ggACTGCGGCATAGGCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                    <  1:1532156/150‑1 (MQ=255)
                                                                                                                    gactGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                   <  2:202235/147‑1 (MQ=255)
                                                                                                                    gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                   <  1:3722944/150‑1 (MQ=255)
                                                                                                                          ggCATTGGCGTGTGCGCCCCCGCCCCCTAACCCTCGCCCTCCGGGAGCGGGGCCCGTTCGGCGCTGTATGTACGCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCGCGCCCCTCGGGGGAGAGGGCCGGGGTGAGGGGaaaa                             <  1:978594/150‑1 (MQ=255)
                                                                                                                          ggCATAGTCGTTTGCGCCCCCTCACCCTACCCCGCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                             <  2:312099/150‑1 (MQ=255)
                                                                                                                          ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                             <  2:8887710/150‑1 (MQ=255)
                                                                                                                          ggCATAGTCGTTTGCGCCCCCACACCCTAACCCTCTCCCTCTGGGTGAGGGGACCGTTCGGCGCTGTATTTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTGAGGGGaaaa                             <  1:2409107/150‑1 (MQ=255)
                                                                                                                            cttAGTGGTTTGGGCCCCCTCCCCCTAACCCGCTCCCTCCGGGAGTGGGGACCGTTCGGGGCTGTTTGTACTCCCTCACTCTGAAACTACACCGAACTCTTTTTTTCTCCCTCGCCCCTCCGGGTTGTGGGCCGGGGTTTGGTGAAAAgg                           <  2:9341728/148‑1 (MQ=255)
                                                                                                                            cATAGACGCTTGCGCCCCCACACCCTAACCCGCTCCCTCAGGGAGAGGGGCCCGTTCGGCGCGGTATGTCCGCCCGCCCTCTGAAACGACACCGCACTCTCTTTTTCTCCCTCGCCCCCCCGGGGAGAGGGCCGGGGTGTGGGGAAAAgg                           <  2:5471199/150‑1 (MQ=255)
                                                                                                                                    tttGCGCCCCCTCACCCTAAACCTAACCCTCAGGGAGAGGGGACCGTTAGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTTTTTTTATCCCTAGCCCCTACGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAcag                   >  2:6989558/1‑148 (MQ=255)
                                                                                                                                       gcgcCCCCACACCCAAACCCACACCCACAGGGAGAGGGGACCGTTCGGCGCTGTATTTACTCCCACACTCTGAAACGACACCCCACTCTTTTTTTATCCCACGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTc                >  1:8150397/1‑150 (MQ=255)
                                                                                                                                              cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt         >  2:10322623/1‑150 (MQ=255)
                                                                                                                                              cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTGTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCGCACCAAtt         >  2:1099362/1‑150 (MQ=255)
                                                                                                                                                   ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCAGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg    >  1:10992357/1‑150 (MQ=255)
                                                                                                                                                     tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt  >  1:8240226/1‑150 (MQ=255)
                                                                                                                                                     tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt  >  2:2661371/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCT  >  NC_000913/3780889‑3781187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: