Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,528,988 G→A 5.2% A419V (GCG→GTG)  sgcC ← putative PTS system EIIC permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,528,9880GA 5.2% 273.4 / 3.8 77A419V (GCG→GTG) sgcCputative PTS system EIIC permease component
Reads supporting (aligned to +/- strand):  ref base G (37/36);  new base A (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

AATGCACGTAGATGGCACATGGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGT  >  NC_000913/4528850‑4529126
                                                                                                                                          |                                                                                                                                          
aaTGCACGTAGATGGCACATGGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGc                                                                                                                                           <  1:1454557/140‑1 (MQ=255)
       gTAGATGGCACATGGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCACCACGACa                                                                                                                                    >  2:257892/1‑140 (MQ=255)
       gTAGATGGCACATGGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCACCACGACa                                                                                                                                    >  2:258293/1‑140 (MQ=255)
         aGATGGCACATGGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAAc                                                                                                                                  <  1:266227/140‑1 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  >  1:661247/1‑140 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  >  1:83500/1‑140 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  >  2:1501803/1‑140 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  >  2:1501402/1‑140 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  <  1:812153/140‑1 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  <  1:489016/140‑1 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  <  1:1330946/140‑1 (MQ=255)
                         tGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGc                                                                                                                  <  1:811872/140‑1 (MQ=255)
                              gCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACAt                                                                                                             <  2:1394160/140‑1 (MQ=255)
                              gCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACAt                                                                                                             <  2:297131/140‑1 (MQ=255)
                              gCTTTTTCCGTCCCAATAACCTCATTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACAt                                                                                                             <  1:1208329/140‑1 (MQ=255)
                                     ccGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCa                                                                                                      >  2:200890/1‑140 (MQ=255)
                                     ccGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTAATCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCATTAATCCCAAGCGACATCAATTCa                                                                                                      >  2:749847/1‑140 (MQ=255)
                                     ccGTCCCAATAACCTCTATCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCa                                                                                                      >  2:749769/1‑140 (MQ=255)
                                       gTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGa                                                                                                    >  2:1572927/1‑140 (MQ=255)
                                        tCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGaa                                                                                                   >  2:746183/1‑140 (MQ=255)
                                          ccAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAAt                                                                                                 >  1:852839/1‑140 (MQ=255)
                                              tAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGAc                                                                                             <  1:287406/140‑1 (MQ=255)
                                              tAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGAc                                                                                             <  2:537432/140‑1 (MQ=255)
                                              tAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGAc                                                                                             <  1:342691/140‑1 (MQ=255)
                                                  ctctTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAAc                                                                                         <  2:1442872/140‑1 (MQ=255)
                                                    ctctCAGCCACCTCATTGAAAGTACTCCTTATTCCGATAATGAACGTTTACGTAAGAACAGCCCGACGCTGGCGACAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACCTCAATTCAGACATCGACCAACCg                                                                                       <  2:1301462/137‑1 (MQ=255)
                                                    ctTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCg                                                                                       >  1:250410/1‑140 (MQ=255)
                                                    ctTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCg                                                                                       <  1:1344891/140‑1 (MQ=255)
                                                     tttCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGa                                                                                      >  2:307501/1‑140 (MQ=255)
                                                      ttCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCTCGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGaa                                                                                     >  2:805779/1‑140 (MQ=255)
                                                      ttCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGaa                                                                                     >  2:673876/1‑140 (MQ=255)
                                                      ttCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTAAGAAATCGACCAACCGaa                                                                                     >  2:673870/1‑140 (MQ=255)
                                                           ccAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACatat                                                                                <  2:1304935/140‑1 (MQ=255)
                                                            cAACTCATTGAAAGTACTCCTTATTCCGATAATTAACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATAtt                                                                               <  2:1014792/140‑1 (MQ=255)
                                                                tCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGccc                                                                           <  1:648307/140‑1 (MQ=255)
                                                                tCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGccc                                                                           <  1:248781/140‑1 (MQ=255)
                                                                    tGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACgg                                                                       >  1:880984/1‑140 (MQ=255)
                                                                     gAAAGTACTCCTTCTTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGa                                                                      <  1:415198/140‑1 (MQ=255)
                                                                     gAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGa                                                                      <  1:1639343/140‑1 (MQ=255)
                                                                        aGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACaa                                                                   >  1:1407556/1‑140 (MQ=255)
                                                                         gTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACTGACAAc                                                                  >  2:26153/1‑140 (MQ=255)
                                                                         gTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAAc                                                                  >  2:26494/1‑140 (MQ=255)
                                                                         gTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAAc                                                                  >  2:26374/1‑140 (MQ=255)
                                                                            cTCCTTCTTCCGATACTTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCTAGCGACATCAATTCAGAAATCGACGAACCGAACATATTGCCCACGGACAACGCt                                                               <  2:1207068/140‑1 (MQ=255)
                                                                            cTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCTCGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCt                                                               <  1:891914/140‑1 (MQ=255)
                                                                            cTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCTAACAAATAGACCACGGACAActca                                                               >  1:1668138/1‑137 (MQ=255)
                                                                            cTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCt                                                               <  1:610525/140‑1 (MQ=255)
                                                                            cTCCTTATTCCGATAATTCACGATTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCt                                                               <  1:34088/140‑1 (MQ=255)
                                                                             tCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACAGACCGCCACTACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTg                                                              >  2:862927/1‑140 (MQ=255)
                                                                                 tATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGAt                                                          >  1:1012313/1‑140 (MQ=255)
                                                                                    tCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTg                                                       >  2:1614698/1‑140 (MQ=255)
                                                                                       gATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTgcgc                                                    >  1:1362839/1‑140 (MQ=255)
                                                                                        aTAATTCACGTTTACGTAAGTACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCa                                                   >  2:715733/1‑140 (MQ=255)
                                                                                        aTAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCa                                                   >  2:490819/1‑140 (MQ=255)
                                                                                        aTAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCa                                                   >  2:490888/1‑140 (MQ=255)
                                                                                         tAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCAc                                                  >  1:857572/1‑140 (MQ=255)
                                                                                         tAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCAc                                                  >  1:856750/1‑140 (MQ=255)
                                                                                         tAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCAc                                                  >  1:854807/1‑140 (MQ=255)
                                                                                             tCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTc                                              <  2:354322/140‑1 (MQ=255)
                                                                                                 gTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAAGTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCg                                          <  1:454982/140‑1 (MQ=255)
                                                                                                   ttACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGc                                        <  1:1334582/140‑1 (MQ=255)
                                                                                                   ttACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGc                                        <  1:1334576/140‑1 (MQ=255)
                                                                                                   ttACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGc                                        <  1:1138195/140‑1 (MQ=255)
                                                                                                      cGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCaaa                                     >  1:1446660/1‑140 (MQ=255)
                                                                                                                 cACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGc                          >  2:26496/1‑140 (MQ=255)
                                                                                                                 cACGACGCTGGCGACAAAACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGc                          >  2:981548/1‑140 (MQ=255)
                                                                                                                        cTGGCGACAATACCGACCACCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAg                   <  1:257892/140‑1 (MQ=255)
                                                                                                                        cTGGCGACAATACCGACCACCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAg                   <  1:258293/140‑1 (MQ=255)
                                                                                                                           gCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAgcgc                >  1:345189/1‑140 (MQ=255)
                                                                                                                             gACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCa              <  2:80969/140‑1 (MQ=255)
                                                                                                                             gACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCa              <  2:591183/140‑1 (MQ=255)
                                                                                                                                aaTACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCAttt           <  2:358355/140‑1 (MQ=255)
                                                                                                                                aaTACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATACAGAACTCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCAttt           <  2:1644928/140‑1 (MQ=255)
                                                                                                                                aaTACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAAATCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAATCTGAAGCCGCGGGTGAGCGCCAgtt           >  2:1337606/1‑140 (MQ=255)
                                                                                                                                aaTACAGACCGCCACGAAAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACGTTCACCGGCAATACTGAAACCGCGTTTGAGCGCCCttg           >  2:1337607/1‑139 (MQ=255)
                                                                                                                                  tACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGACATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCa         <  2:661247/140‑1 (MQ=255)
                                                                                                                                  tACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAACTCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCa         <  1:60119/140‑1 (MQ=255)
                                                                                                                                     cgaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGATCATATTTCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTg      <  1:1616218/140‑1 (MQ=255)
                                                                                                                                     cgaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTg      <  1:314405/140‑1 (MQ=255)
                                                                                                                                     cgaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTg      >  2:721860/1‑140 (MQ=255)
                                                                                                                                     cgaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCAACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTg      <  2:1238811/140‑1 (MQ=255)
                                                                                                                                      gaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCGTTTGAGCGCCATTTctggga     >  1:1348587/1‑135 (MQ=255)
                                                                                                                                      gaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGa     >  1:1173091/1‑140 (MQ=255)
                                                                                                                                       accgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGTGCGCCATTTCAGTGaa    >  2:803630/1‑140 (MQ=255)
                                                                                                                                       accgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGaa    >  1:84149/1‑140 (MQ=255)
                                                                                                                                       accgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGaa    >  2:1238240/1‑140 (MQ=255)
                                                                                                                                       accgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGaa    >  1:1114024/1‑140 (MQ=255)
                                                                                                                                         cgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATACTGCCCACGGGCAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGt  <  2:56719/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AATGCACGTAGATGGCACATGGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGTACTCCTTATTCCGATAATTCACGTTTACGTAAGAACAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGT  >  NC_000913/4528850‑4529126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: