Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	399,838	G→T	6.2%	T362T (ACC→ACA)	ddlA ←	D‑alanine‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,838	0	G	T	6.2%	230.9 / 2.3	64	T362T (ACC→ACA)	ddlA	D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base G (19/41);  new base T (2/2);  total (21/43)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

AAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTG  >  NC_000913/399699‑399976                                                                                                                                            |                                                                                                                                           aaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGAGGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATtgtg                                                                                                                                            >  2:495505/1‑140 (MQ=255)            aCCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGc                                                                                                                                 >  1:45226/1‑140 (MQ=255)              cTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                                               >  1:1396663/1‑140 (MQ=255)                    cGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCg                                                                                                                         <  2:1425325/140‑1 (MQ=255)                      tCGGTCTGGTTACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTAACGGGCGATAAAATATTACATAGAGGTATTCAATGCGTTATCCGCa                                                                                                                       >  1:1051528/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTTGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:43678/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTTGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:43420/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:1026295/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:870792/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:870776/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:1556674/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:1228109/1‑140 (MQ=255)                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTAATCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                                       >  1:870891/1‑140 (MQ=255)                       cGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTCTTCGTCGTCGCGATGCAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATCTTACATTGTGGTTTTCACTGCGTTATCCGCAg                                                                                                                      <  2:1381807/140‑1 (MQ=255)                          tCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATTAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGt                                                                                                                   <  1:1683693/140‑1 (MQ=255)                            tGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGa                                                                                                                 <  1:1146709/140‑1 (MQ=255)                            tGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGa                                                                                                                 <  1:902421/140‑1 (MQ=255)                                    tGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAg                                                                                                         >  2:1385075/1‑140 (MQ=255)                                        tGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGcc                                                                                                     >  1:1549576/1‑140 (MQ=255)                                        tGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGcc                                                                                                     >  1:785118/1‑140 (MQ=255)                                         gATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCa                                                                                                    >  2:907550/1‑140 (MQ=255)                                         gATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCa                                                                                                    >  2:907479/1‑140 (MQ=255)                                                ttCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaat                                                                                             >  1:26231/1‑140 (MQ=255)                                                 tCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatc                                                                                            >  1:23826/1‑140 (MQ=255)                                                            gggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTTGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGAt                                                                                 <  2:43420/140‑1 (MQ=255)                                                            gggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTTGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGAt                                                                                 <  2:43678/140‑1 (MQ=255)                                                            gggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGAt                                                                                 <  1:113608/140‑1 (MQ=255)                                                                 gagtattCGTCGTCTCGATGAAGAAACTGAAAACGACCAATAAGAATTTTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTGATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  2:1254655/137‑1 (MQ=255)                                                                 gaatattCGTCGTCGCGATGAAGTAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  1:758703/137‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  2:295785/140‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  1:1005261/140‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  2:346287/140‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  2:1053411/140‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  2:1052994/140‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  1:993431/140‑1 (MQ=255)                                                                 gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                                            <  1:953245/140‑1 (MQ=255)                                                                                   tGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCTATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCGAGACCGc                                                          <  2:970967/140‑1 (MQ=255)                                                                                             gAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCca                                                <  2:583638/140‑1 (MQ=255)                                                                                             gAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCca                                                <  2:583664/140‑1 (MQ=255)                                                                                                   gCCCAATAAGAATATTATCGGGCGTTTAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCtt                                          <  2:801363/140‑1 (MQ=255)                                                                                                   gCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCtt                                          <  1:10540/140‑1 (MQ=255)                                                                                                   gCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCtt                                          <  2:801364/140‑1 (MQ=255)                                                                                                   gCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCtt                                          <  1:691250/140‑1 (MQ=255)                                                                                                       aaTAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGa                                      <  1:1100272/140‑1 (MQ=255)                                                                                                            gAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacat                                 <  2:1423813/140‑1 (MQ=255)                                                                                                             aaTATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacata                                <  1:453931/140‑1 (MQ=255)                                                                                                             aaTATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGATTCGGatacata                                <  1:453772/140‑1 (MQ=255)                                                                                                              attttttCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacatac                               <  2:1067826/134‑1 (MQ=255)                                                                                                              atatTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTATCCCAGACCGCTGGCTTGCCACAGCTTCGGatacatac                               <  2:1068117/140‑1 (MQ=255)                                                                                                               tattatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACt                              <  2:123364/140‑1 (MQ=255)                                                                                                                   atCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGata                          <  1:538570/140‑1 (MQ=255)                                                                                                                       ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAg                      >  1:922987/1‑140 (MQ=255)                                                                                                                       ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAg                      <  2:458368/140‑1 (MQ=255)                                                                                                                       ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAg                      >  1:382203/1‑140 (MQ=255)                                                                                                                       ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAg                      <  1:1170720/140‑1 (MQ=255)                                                                                                                        gCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGt                     <  1:188899/140‑1 (MQ=255)                                                                                                                        gCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGt                     <  1:188926/140‑1 (MQ=255)                                                                                                                              aaaTATTACATAGTGGTTATCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGAACAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGcc               <  2:1647151/140‑1 (MQ=255)                                                                                                                                 tatTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcagg            <  1:699196/140‑1 (MQ=255)                                                                                                                                 tatTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGATTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcagg            <  1:699736/140‑1 (MQ=255)                                                                                                                                  atTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcaggc           <  2:334575/140‑1 (MQ=255)                                                                                                                                  atTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcaggc           <  2:334689/140‑1 (MQ=255)                                                                                                                                   ttACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcaggca          <  1:1426418/140‑1 (MQ=255)                                                                                                                                   ttACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcaggca          <  2:26231/140‑1 (MQ=255)                                                                                                                                     aCATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCcaggcagg        >  2:1475351/1‑140 (MQ=255)                                                                                                                                         tgtgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGgtgt    >  2:916317/1‑140 (MQ=255)                                                                                                                                           tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTg  >  1:369960/1‑140 (MQ=255)                                                                                                                                           tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTg  >  1:372372/1‑140 (MQ=255)                                                                                                                                           tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTg  >  1:1645520/1‑140 (MQ=255)                                                                                                                                           tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTg  >  1:537705/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                           AAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTG  >  NC_000913/399699‑399976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑