Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 517,801 G→A 5.6% A160V (GCG→GTG)  ybbN ← DnaK co‑chaperone, thioredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913517,8010GA 5.6% 250.3 / 3.5 71A160V (GCG→GTG) ybbNDnaK co‑chaperone, thioredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (33/34);  new base A (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCCGGCGTATCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCCTGCTGCGCTTTCAG  >  NC_000913/517678‑517934
                                                                                                                           |                                                                                                                                     
tCCGGCGTATCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGc                                                                                                                       <  1:683406/140‑1 (MQ=255)
 ccGGCGTATCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCGTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGTACGGTTCAGCGCAATCAGCGTTTCTGcc                                                                                                                      >  2:1160101/1‑140 (MQ=255)
   ggCGTATCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCcag                                                                                                                    >  1:1259874/1‑140 (MQ=255)
   ggCGTATCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCcag                                                                                                                    >  1:1259709/1‑140 (MQ=255)
   ggCGTATCAGCCGCCAGCTTCAGCAGTTCGAATAGCCCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGAATCCGCATCTACAGAACGGTTCAGCGCAATCAGCGTTTCTGCcag                                                                                                                    >  1:1259587/1‑140 (MQ=255)
         tCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCcagcagcag                                                                                                              >  1:29337/1‑140 (MQ=255)
              cgccTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGa                                                                                                         <  1:1516991/140‑1 (MQ=255)
              cgacTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTTGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGACAGCATCAGGCCGa                                                                                                         <  1:363968/137‑1 (MQ=255)
                 cTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGAtct                                                                                                      >  2:868253/1‑140 (MQ=255)
                  tGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGAtctc                                                                                                     <  1:1347085/140‑1 (MQ=255)
                   gCTTCAGCAGTTCGATTTGCGCCACCAGCCCCAGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTcc                                                                                                    <  2:659433/140‑1 (MQ=255)
                             ttCGATTTGCGCCACCAGCCCCTGTTAGCGGGTGTCCTGATCCTGCAACGGAGTGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGAt                                                                                          >  1:1140455/1‑140 (MQ=255)
                             ttCGATTTGCGCCACCAGCCCCTGTTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGAt                                                                                          >  1:1089420/1‑140 (MQ=255)
                               cGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTc                                                                                        >  1:1114008/1‑140 (MQ=255)
                                  tttGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGAc                                                                                     <  1:1239431/140‑1 (MQ=255)
                                      cgcCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACt                                                                                 >  2:80263/1‑140 (MQ=255)
                                            caGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAgg                                                                           <  2:361153/140‑1 (MQ=255)
                                            caGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAgg                                                                           <  2:361072/140‑1 (MQ=255)
                                            caGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAgg                                                                           <  1:1397916/140‑1 (MQ=255)
                                                    ggTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCACAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTc                                                                   >  2:860203/1‑140 (MQ=255)
                                                    ggTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCACAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTc                                                                   >  2:860073/1‑140 (MQ=255)
                                                       aGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGc                                                                <  1:428313/140‑1 (MQ=255)
                                                         cGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATAAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCaa                                                              >  1:1080242/1‑140 (MQ=255)
                                                            gtgtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCACTCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATgg                                                           <  2:788157/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  1:25581/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  1:25631/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  1:26657/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  1:25569/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  2:1065390/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCACAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  1:860203/140‑1 (MQ=255)
                                                              gtCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCACAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCa                                                         <  1:860073/140‑1 (MQ=255)
                                                               tCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAg                                                        >  1:467546/1‑140 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  2:1207087/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  1:1158044/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  2:634811/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  2:558830/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  2:557387/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  1:1223154/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  2:163823/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  1:453583/140‑1 (MQ=255)
                                                                    aTCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  1:78145/140‑1 (MQ=255)
                                                                    aTCCAGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGTTTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCAt                                                   <  2:634442/140‑1 (MQ=255)
                                                                     tCCTGCAACGGATTGGTCTTCAGGACCGCTTCCGCATCTTCACAACGGTTCAGCGCAAACTGCGTTTCAGCCAGCAGCAGGCCGATCTCCCCGATCAGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATc                                                  <  2:1166487/140‑1 (MQ=255)
                                                                        tGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgt                                               >  1:1098554/1‑140 (MQ=255)
                                                                        tGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgt                                               >  1:1100227/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCTTCTTCAGAACTGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCTGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTGTTTCAGCTATGGCAGGGCATAGgtg                                              >  2:1565649/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtg                                              >  1:1465165/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtg                                              >  1:1178214/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtg                                              >  1:604511/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtg                                              >  1:1177630/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtg                                              >  2:1439946/1‑140 (MQ=255)
                                                                         gCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtg                                              >  1:626273/1‑140 (MQ=255)
                                                                          cAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGTGCATCGgtgt                                             <  1:649060/140‑1 (MQ=255)
                                                                          cAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGgtgt                                             <  1:1227112/140‑1 (MQ=255)
                                                                                  tGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCACGCCGAACTCCCCGTTCTGAAACGACAACTGCCAGGCGTCATTCAGCAATGGCAGGGCATCGGTGAAATTTCtt                                     >  2:1466883/1‑140 (MQ=255)
                                                                                        tCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGc                               >  1:1665276/1‑140 (MQ=255)
                                                                                        tCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGc                               <  2:29337/140‑1 (MQ=255)
                                                                                        tCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGc                               >  1:1665193/1‑140 (MQ=255)
                                                                                               cGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGtt                        >  2:374543/1‑140 (MQ=255)
                                                                                                 cTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGc                      >  1:1043627/1‑140 (MQ=255)
                                                                                                 cTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGc                      >  1:475020/1‑140 (MQ=255)
                                                                                                 cTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGc                      >  2:405159/1‑140 (MQ=255)
                                                                                                 cTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGc                      >  2:1499722/1‑140 (MQ=255)
                                                                                                            tCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCctgctg           >  2:1553195/1‑140 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCaa                                                              <  2:540778/80‑1 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCaa                                                              >  1:540778/1‑80 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATTGCCTGCTGCGCTTTCAg  >  2:809559/1‑140 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCCTGCTGCGCTTTCAg  >  1:240593/1‑140 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCCTGCTGCGCTTTCAg  <  1:875654/140‑1 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCCTGCTGCGCTTTCAg  <  1:875651/140‑1 (MQ=255)
                                                                                                                     ttCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCCTGCTGCGCTTTCAg  <  1:875544/140‑1 (MQ=255)
                                                                                                                           |                                                                                                                                     
TCCGGCGTATCAGCCGCCTGCTTCAGCAGTTCGATTTGCGCCACCAGCCCCTGGTAGCGGGTGTCCTGATCCTGCAACGGAATGGTTTTCAGCACCGCTTCCGCATCTTCAGAACGGTTCAGCGCAATCAGCGTTTCTGCCAGCAGCAGGCCGATCTCCCCGTTCTGATTCGACAACTGCCAGGCGTCTTTCAGCAATGGCAGGGCATCGGTGTAATTGCTTTCCTGCATCAGTTGCATCGCCTGCTGCGCTTTCAG  >  NC_000913/517678‑517934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: