Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 566,173 C→G 16.5% pseudogene (204/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,1730CG16.5% 262.1 / 44.5 85pseudogene (204/279 nt)exoDpseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (19/52);  new base G (9/5);  total (28/57)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ACTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAC  >  NC_000913/566034‑566302
                                                                                                                                           |                                                                                                                                 
aCTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCAcc                                                                                                                                   >  1:402894/1‑140 (MQ=255)
   gcaatctggttcgccagttgtggcggtaagacctgaattggcattttgtaatcctcctctcTCATCGCCATTGCGCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGAtc                                                                                                                                <  2:379417‑M1/79‑1 (MQ=255)
    aaGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCAtct                                                                                                                               >  2:1368569/1‑140 (MQ=255)
     aGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCAACGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCAtctc                                                                                                                              >  2:1546416/1‑140 (MQ=255)
          cgcAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCaa                                                                                                                         >  2:654530/1‑140 (MQ=255)
          cgcAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCaa                                                                                                                         >  2:376815/1‑140 (MQ=255)
             tcgccagttgtggcggtaagacctgaattggcattttgtaatcctcctctcTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAAc                                                                                                                      >  1:373989‑M1/52‑140 (MQ=255)
             tcgccagttgtggcggtaagacctgaattggcattttgtaatcctcctctcTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAAc                                                                                                                      >  1:168804‑M1/52‑140 (MQ=255)
             tcgccagttgtggcggtaagacctgaattggcattttgtaatcctcctctcTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAAc                                                                                                                      >  1:70512‑M1/52‑140 (MQ=255)
             tcgccagttgtggcggtaagacctgaattggcattttgtaatcctcctctcTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAAc                                                                                                                      >  1:1352595‑M1/52‑140 (MQ=255)
             attGCAGGCTTGCCGTGTTGTGAGGTACCAGGATATTATCGTGAGGATGCGTCATCGCCATTGATACCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCTTTTTTTTGATGATATCAGGCACCATCTCGTCAAAAc                                                                                                                      <  1:1612041/138‑1 (MQ=255)
             aaTGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAAc                                                                                                                      <  1:1430127/140‑1 (MQ=255)
             aaTGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAAc                                                                                                                      <  1:1427176/140‑1 (MQ=255)
             aaTGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAAc                                                                                                                      <  1:1473025/140‑1 (MQ=255)
               tGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTc                                                                                                                    <  2:470215/140‑1 (MQ=255)
               tGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTc                                                                                                                    >  2:691982/1‑140 (MQ=255)
                  aGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGcc                                                                                                                 <  1:551306/140‑1 (MQ=255)
                  aGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGcc                                                                                                                 <  1:1444198/140‑1 (MQ=255)
                  aGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGcc                                                                                                                 <  1:1081342/140‑1 (MQ=255)
                      ttGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGt                                                                                                             <  2:1484373/140‑1 (MQ=255)
                      ttGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGt                                                                                                             <  2:547348/140‑1 (MQ=255)
                         ccGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACt                                                                                                          >  2:1251464/1‑140 (MQ=255)
                         ccGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACt                                                                                                          >  1:985466/1‑140 (MQ=255)
                         acGTGTTGTGAGGTACCCGGATATTATCGTGAGGAAGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCTTCCATTTTTTCTATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACt                                                                                                          >  2:100841/2‑140 (MQ=255)
                                    ggTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGc                                                                                               <  1:1263485/140‑1 (MQ=255)
                                    ggTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGc                                                                                               <  1:1382886/140‑1 (MQ=255)
                                     gTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCt                                                                                              <  1:1661798/140‑1 (MQ=255)
                                        cccGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCaa                                                                                           >  2:917804/1‑140 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACACAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:535304/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGCTGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:534572/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  2:1232914/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  2:1092247/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  2:537221/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:620493/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  2:730163/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:1109366/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:1128187/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:1523905/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:1487860/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:311770/140‑1 (MQ=255)
                                           ggATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCATCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCa                                                                                        <  1:850540/140‑1 (MQ=255)
                                           gcatatTATCGTGAGGATGCGTCAACGCCATTGCTCCCCAAAAACAAAACCAATTTCAGCCAGTGCCTCGTCCATATTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACATTTCATTCCGCTCAATCa                                                                                        <  2:253996/138‑1 (MQ=255)
                                                ttatCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACa                                                                                   <  1:304180/140‑1 (MQ=255)
                                                ttatCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACa                                                                                   <  1:1564855/140‑1 (MQ=255)
                                                ttatCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACa                                                                                   <  1:118237/140‑1 (MQ=255)
                                                     atcctcctctcTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATg                                                                              >  1:1239356‑M1/12‑140 (MQ=255)
                                                      tGAGGATGCGTCATCGCCATTGCTCCCCCAATACAAAACCAATTTCCGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGc                                                                             <  2:775706/140‑1 (MQ=255)
                                                      tGAGGATGCGTCATCGCCATTGCTCCCCAACTACACAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGc                                                                             <  2:777146/140‑1 (MQ=255)
                                                      tGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGc                                                                             >  1:315306/1‑140 (MQ=255)
                                                      tGAGGATGCGTCATCGCCATTGCTACCCAAATACAAAACCAATTGCAGCCAGTGCCTCGTCCATTTTTACGATGAACACCGGCACCCTCTCGTCAAAACTCGCCATGTACTTATCAATCCGCTCAATCACGACAAAAtcc                                                                             >  1:22808/1‑138 (MQ=255)
                                                        aGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAg                                                                           >  1:1640561/1‑140 (MQ=255)
                                                           aTGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATATTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGcc                                                                        <  1:453858/140‑1 (MQ=255)
                                                               gTCATCGCCATTGCTCCCCAACTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCa                                                                    <  1:1502804/140‑1 (MQ=255)
                                                               gTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCa                                                                    <  1:918864/140‑1 (MQ=255)
                                                               gTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCa                                                                    <  2:864158/140‑1 (MQ=255)
                                                               cTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCa                                                                    >  1:1385591‑M1/2‑140 (MQ=255)
                                                               cTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCa                                                                    >  1:435251‑M1/2‑140 (MQ=255)
                                                                          tGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                         <  1:1383139/140‑1 (MQ=255)
                                                                          tGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATgcg                                                         <  1:1519905/140‑1 (MQ=255)
                                                                             tCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCgg                                                      >  2:662237/1‑140 (MQ=255)
                                                                              ccccAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGTTGAACTCCGGCACGATCTCGTCAAATCTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCggg                                                     >  1:296102/1‑140 (MQ=255)
                                                                                 cAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCa                                                  >  1:437260/1‑140 (MQ=255)
                                                                                          aaCCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCa                                         <  1:371930/140‑1 (MQ=255)
                                                                                          aaCCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCa                                         <  1:1312181/140‑1 (MQ=255)
                                                                                          aaCCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCa                                         <  1:1368569/140‑1 (MQ=255)
                                                                                           aCCAATTTCAGCCAGGGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCaa                                        <  1:492785/140‑1 (MQ=255)
                                                                                               aTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTa                                    >  2:1132194/1‑140 (MQ=255)
                                                                                               aTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTa                                    >  2:1132190/1‑140 (MQ=255)
                                                                                               aTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTa                                    >  2:875527/1‑140 (MQ=255)
                                                                                                 ttCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTAcc                                  <  1:358429/140‑1 (MQ=255)
                                                                                                    aGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAgg                               >  2:1059407/1‑140 (MQ=255)
                                                                                                               cGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTcgc                    >  2:1480649/1‑140 (MQ=255)
                                                                                                                   cATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCa                <  1:1320088/140‑1 (MQ=255)
                                                                                                                    acctgttCGCTGCACTCCCGCACCCGCTCGTCAAACCTCGCCAGGTACTTTTCCGTCCGCTCACTCCCGACATCATGCCGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAc               <  2:804530/135‑1 (MQ=255)
                                                                                                                    aTTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAc               >  2:49447/1‑140 (MQ=255)
                                                                                                                      tttttCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACAAAATGCACGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCAccc             <  1:371248/140‑1 (MQ=255)
                                                                                                                      tttttCGAAGAACACCGGCACCATCACGTCAAAACTCGCCATCTACTTTTCATTCCGCTCAAACAAGACATAATGCAGGCCTTCACGCTTCAAGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTGTCGCGTCAccc             <  2:1267221/140‑1 (MQ=255)
                                                                                                                       ttttCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCca            <  1:1556322/140‑1 (MQ=255)
                                                                                                                        tttCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac           <  1:231656/140‑1 (MQ=255)
                                                                                                                        tttCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac           <  1:1425705/140‑1 (MQ=255)
                                                                                                                        tttCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATCTACTTTTCATTCCGCACAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac           <  1:640448/140‑1 (MQ=255)
                                                                                                                           cGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg        <  2:777349/140‑1 (MQ=255)
                                                                                                                                 aCTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATACTGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTAc  <  1:202975/140‑1 (MQ=255)
                                                                                                                                 aCTCCGGCACCATCTCGTCTAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc  <  1:1132190/140‑1 (MQ=255)
                                                                                                                                 aCTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc  <  1:1132194/140‑1 (MQ=255)
                                                                                                                                 aCTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc  <  2:1055641/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                 
ACTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAC  >  NC_000913/566034‑566302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: