Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 738,514 A→G 5.6% G141G (GGA→GGG ybfD → H repeat‑associated putative transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913738,5140AG 5.6% 263.2 / 6.8 90G141G (GGA→GGGybfDH repeat‑associated putative transposase
Reads supporting (aligned to +/- strand):  ref base A (58/26);  new base G (3/2);  total (61/29)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

TTGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGG  >  NC_000913/738375‑738653
                                                                                                                                           |                                                                                                                                           
ttGACTACCATTCTTCAGATGATAAATAAGTCATCGCATTTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  2:397970/140‑1 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTGTGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  1:1428920/140‑1 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  1:1004450/140‑1 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             >  1:328700/1‑140 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  1:510050/140‑1 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  1:1062372/140‑1 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  1:1062486/140‑1 (MQ=255)
ttGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                                                                             <  2:1403835/140‑1 (MQ=255)
     tagctttcttcAGTTGTTAAAGTGGTCATGGCAATTGTTGGTAATTTACACCGGCATTCTTATGACAAGAGTCGGGGTATGGGAGCGTTTCATGTGATTTGTGCGTTGTCAACAATGCACAGTCTGGTCTTGGGACAGAt                                                                                                                                        >  2:258940/6‑140 (MQ=9)
       ccATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCa                                                                                                                                      >  1:838169/1‑140 (MQ=255)
       ccATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCa                                                                                                                                      >  1:1458071/1‑140 (MQ=255)
       ccATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAAAACACCGGCATTCTTATGACAAGAGTCGCCGGAAGGGAGCGAGTCAAGTCATTAGTGCGTTCTCAACAATGCCCAGACTGGTCAACGGACAGATCa                                                                                                                                      >  2:923052/1‑140 (MQ=14)
        cATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCaa                                                                                                                                     <  2:903630/140‑1 (MQ=255)
                  atgatAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAg                                                                                                                           >  2:1577496/1‑140 (MQ=255)
                  atgatAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAg                                                                                                                           <  2:1143130/140‑1 (MQ=255)
                       aaaGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATc                                                                                                                      >  1:566378/1‑140 (MQ=255)
                       aaaGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATc                                                                                                                      >  1:566334/1‑140 (MQ=255)
                       aaaGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATc                                                                                                                      >  1:489024/1‑140 (MQ=255)
                           aCGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAAt                                                                                                                  <  1:564846/140‑1 (MQ=255)
                               cATCGCAATTGAGGGAAAAATACCCCGGCATTCTTATGACAAGAGTCGCCGGAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATgaga                                                                                                              >  1:1451713/1‑140 (MQ=255)
                                aTCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGAt                                                                                                             >  1:1433084/1‑140 (MQ=255)
                                aTCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGAt                                                                                                             >  1:1416862/1‑140 (MQ=255)
                                aTCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGAt                                                                                                             >  1:140211/1‑140 (MQ=255)
                                         gttGGAAAAATACACCTGCATTATTATGACAAGAGTCGCCGTAATGGAGCTATTAATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTAt                                                                                                    <  2:67909/138‑1 (MQ=14)
                                              aaaaaTACACCGGCATTCTTATGACAAGTGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTg                                                                                               >  1:442324/1‑140 (MQ=255)
                                              aaaaaTACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTg                                                                                               <  1:1604229/140‑1 (MQ=255)
                                                aaaTACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGaa                                                                                             <  2:1411509/140‑1 (MQ=255)
                                                       ccGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTa                                                                                      <  1:1577496/140‑1 (MQ=40)
                                                                  aTGACAAGTGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTGTCTACAATGCACAGTCTGGTCATCGGACAGATCAAGTCGGATAAGTAATCCAATGAGATCACTGCTATCGCTGTACTTCTTATCATGCTGGat                                                                           >  2:1479850/1‑140 (MQ=11)
                                                                  aTGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCATGACGGATAAGAAATCCAATGTGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat                                                                           >  2:1479816/1‑140 (MQ=255)
                                                                  aTGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat                                                                           >  1:1223757/1‑140 (MQ=37)
                                                                  aTGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat                                                                           >  2:316259/1‑140 (MQ=37)
                                                                  aTGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat                                                                           >  2:723248/1‑140 (MQ=37)
                                                                    gACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGatat                                                                         <  2:888754/140‑1 (MQ=37)
                                                                     aCAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATAtt                                                                        >  1:1619003/1‑140 (MQ=37)
                                                                          agTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAgg                                                                   >  1:841578/1‑140 (MQ=37)
                                                                              gCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGaaaa                                                               <  1:954588/140‑1 (MQ=37)
                                                                               ccGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGaaaaa                                                              <  2:1433084/140‑1 (MQ=37)
                                                                                   aaGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAatcat                                                          <  1:319951/140‑1 (MQ=33)
                                                                                    aGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAAGGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAatcatc                                                         >  1:1143366/1‑140 (MQ=18)
                                                                                         gCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAAc                                                    >  1:95879/1‑140 (MQ=34)
                                                                                         gCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCAAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAATATCATCAAAAc                                                    >  1:547411/1‑140 (MQ=21)
                                                                                          cGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAAcc                                                   >  1:333438/1‑140 (MQ=35)
                                                                                          cGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAAcc                                                   <  2:1517756/140‑1 (MQ=35)
                                                                                          cGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAAcc                                                   >  1:92334/1‑140 (MQ=35)
                                                                                          cGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAAGGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAAcc                                                   >  1:776514/1‑140 (MQ=25)
                                                                                          cGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAAGCCAAGGAGATCACCGCTATCCCTGAACTTCTTAACATGCTGGAGATTAAAGGAAAAATCCGCAAAAcc                                                   >  1:92224/1‑140 (MQ=11)
                                                                                            aTTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACcga                                                 >  1:497818/1‑140 (MQ=37)
                                                                                            aTTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACcga                                                 >  1:1319190/1‑140 (MQ=37)
                                                                                               cATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACcgatgc                                              <  2:1376284/140‑1 (MQ=17)
                                                                                               cATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACcgatgc                                              <  2:1376789/140‑1 (MQ=17)
                                                                                                 tGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACcgatgcga                                            <  2:644447/140‑1 (MQ=37)
                                                                                                     aTTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATggg                                        <  2:353238/140‑1 (MQ=37)
                                                                                                       tattGCGTTCTCAACAATGCACAGTCTGGTCATCGGACATATCAAGACGGATAAGAAATCCAATGATATCACAGCTATCCCTGAACTTCTTAACAAGCTGGATAATAAAGGAAAAATAATCAAAACCGAAGCGATGGGtt                                      >  1:1195409/4‑140 (MQ=11)
                                                                                                       tAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGtt                                      >  1:638151/1‑140 (MQ=37)
                                                                                                       tAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGtt                                      <  2:1174428/140‑1 (MQ=37)
                                                                                                       tAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGtt                                      >  1:417685/1‑140 (MQ=37)
                                                                                                       tAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCACTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGtt                                      <  2:271387/140‑1 (MQ=37)
                                                                                                       tAGTGCGTTCACAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGtt                                      <  2:271474/140‑1 (MQ=37)
                                                                                                         gTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGc                                    <  2:237952/140‑1 (MQ=37)
                                                                                                            cGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAg                                 <  1:434356/140‑1 (MQ=37)
                                                                                                              ttCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGaa                               >  1:189926/1‑140 (MQ=17)
                                                                                                              ttCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGaa                               >  1:189933/1‑140 (MQ=17)
                                                                                                              ttCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGaa                               >  2:1053051/1‑140 (MQ=37)
                                                                                                               tctcAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGaaa                              >  1:433304/1‑140 (MQ=37)
                                                                                                                   aacaaTGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGata                          >  2:648744/1‑140 (MQ=37)
                                                                                                                   aacaaTGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGata                          >  1:538862/1‑140 (MQ=37)
                                                                                                                        tGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCa                     >  2:915002/1‑140 (MQ=37)
                                                                                                                        tGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCa                     >  1:908806/1‑140 (MQ=37)
                                                                                                                        tGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCa                     >  1:279729/1‑140 (MQ=37)
                                                                                                                        tGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCa                     >  1:296663/1‑140 (MQ=37)
                                                                                                                        tGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCa                     >  2:1076835/1‑140 (MQ=37)
                                                                                                                             aGTCTGGTCATCGGTCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaa                <  2:119172/140‑1 (MQ=21)
                                                                                                                             aGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaa                <  2:887398/140‑1 (MQ=37)
                                                                                                                             aGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaa                >  1:169978/1‑140 (MQ=37)
                                                                                                                             aGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaa                <  2:119166/140‑1 (MQ=37)
                                                                                                                               tCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaaga              <  2:940876/140‑1 (MQ=37)
                                                                                                                               tCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaaga              >  2:456200/1‑140 (MQ=37)
                                                                                                                               tCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaaga              >  1:412480/1‑140 (MQ=37)
                                                                                                                               tCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaaga              >  2:322929/1‑140 (MQ=37)
                                                                                                                               tCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGagaaga              >  1:595597/1‑140 (MQ=37)
                                                                                                                                 ttgTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATa            <  2:1357132/138‑1 (MQ=37)
                                                                                                                                  ggTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATAc           >  2:247662/1‑140 (MQ=37)
                                                                                                                                  ggTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATAc           >  2:755823/1‑140 (MQ=37)
                                                                                                                                   gTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACa          >  2:829202/1‑140 (MQ=37)
                                                                                                                                    tCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACaa         <  2:564853/140‑1 (MQ=37)
                                                                                                                                    tCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACaa         <  2:1568952/140‑1 (MQ=37)
                                                                                                                                    tCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACaa         <  2:1355406/140‑1 (MQ=37)
                                                                                                                                       tCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGTAAGATATTGCAGAGAAGATACaaaaa      >  1:155153/1‑140 (MQ=17)
                                                                                                                                       tCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATCCTGGAGATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATAcaacaa      >  2:190574/1‑140 (MQ=255)
                                                                                                                                       tCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCATGAACTTCTAAACATGCTGGATATTAAAGGAAAAAACATCAAAACAGATGCTATGGGTTGCCAGAAAGATATTGCAGAGAAGATACaaaaa      >  1:649480/1‑140 (MQ=11)
                                                                                                                                        cGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAAc     >  1:1641567/1‑140 (MQ=37)
                                                                                                                                         ggACAGATCAAGACGGATAAGAAATCCAATGAGATCACATCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGAGGGGTTGCCAGAAAGATATAGCAGAGAAGATCCAAAAACa    >  2:116944/1‑140 (MQ=11)
                                                                                                                                         ggACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACa    >  2:1397795/1‑140 (MQ=37)
                                                                                                                                         ggACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACa    >  1:1127835/1‑140 (MQ=37)
                                                                                                                                         ggACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACa    >  1:1581241/1‑140 (MQ=37)
                                                                                                                                         ggACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACa    >  1:387496/1‑140 (MQ=37)
                                                                                                                                         ggACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACa    >  1:931285/1‑140 (MQ=37)
                                                                                                                                           aCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAgg  <  1:1438425/140‑1 (MQ=35)
                                                                                                                                           |                                                                                                                                           
TTGACTACCATTCTTCAGATGATAAAGACGTCATCGCAATTGATGGAAAAATACACCGGCATTCTTATGACAAGAGTCGCCGTAAGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCAAAACCGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGG  >  NC_000913/738375‑738653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: