Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 984,140 C→A 6.1% P164Q (CCG→CAG)  ycbL → putative metal‑binding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913984,1400CA 6.1% 379.0 / 6.2 106P164Q (CCG→CAG) ycbLputative metal‑binding enzyme
Reads supporting (aligned to +/- strand):  ref base C (57/42);  new base A (4/3);  total (61/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

AGCATAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTT  >  NC_000913/984001‑984269
                                                                                                                                           |                                                                                                                                 
   aTAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGCTGTTATTTTCAAAGGCGGAGTAGGGCGCAGAGACTTCCcgcg                                                                                                                                >  1:109064/1‑140 (MQ=255)
   aTAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCcgcg                                                                                                                                >  1:108875/1‑140 (MQ=255)
   aTAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCcgcg                                                                                                                                >  1:532280/1‑140 (MQ=255)
   aTAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCcgcg                                                                                                                                >  1:532392/1‑140 (MQ=255)
   aTAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCcgcg                                                                                                                                >  1:1000186/1‑140 (MQ=255)
     aGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCAGCGTg                                                                                                                              >  1:749670/1‑140 (MQ=255)
     aGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCAGCGTg                                                                                                                              >  1:749875/1‑140 (MQ=255)
     aGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCAGCGTg                                                                                                                              >  1:749906/1‑140 (MQ=255)
      gggAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTgg                                                                                                                             >  1:240844/1‑140 (MQ=255)
      gggAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTgg                                                                                                                             >  1:247121/1‑140 (MQ=255)
       ggAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTCGGGCGCAGTGACTTCCCGCGTGGc                                                                                                                            >  2:294283/1‑140 (MQ=255)
       ggAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGc                                                                                                                            >  2:11185/1‑140 (MQ=255)
       ggAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGc                                                                                                                            >  2:11725/1‑140 (MQ=255)
       ggAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGc                                                                                                                            >  1:1424737/1‑140 (MQ=255)
            gtgACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCa                                                                                                                       >  1:943126/1‑140 (MQ=255)
            gtgACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCa                                                                                                                       >  1:943138/1‑140 (MQ=255)
            gtgACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCa                                                                                                                       >  1:430129/1‑140 (MQ=255)
            gtgACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCa                                                                                                                       >  2:98084/1‑140 (MQ=255)
            gtgACTTAACAGGTGTTACATTGCCCAGGGCATACCCCGGGTCCTGTCGAGTTTTATTAAGATCGGGCAAAGCTGCTGATTACTTGCGAGGTTATTTTCAAAGGCGGATAATGTCGCAGTTACTACCAGCCTGGCGATCa                                                                                                                       >  1:757755/1‑140 (MQ=255)
              gACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATa                                                                                                                     <  1:1622825/140‑1 (MQ=255)
              gACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATa                                                                                                                     <  1:262033/140‑1 (MQ=255)
                   tACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCaa                                                                                                                >  1:1553285/1‑140 (MQ=255)
                   tACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCaa                                                                                                                >  1:526068/1‑140 (MQ=255)
                   tACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCaa                                                                                                                >  1:719061/1‑140 (MQ=255)
                           ttACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTAGTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATttc                                                                                                        >  2:1188697/1‑140 (MQ=255)
                           ttACATTGCCCTGGGCATACGCCGGGTCATGACGGGTTGTTTGATCATCGGGCCAAGCTGCTGAAGTCTGGCGAGGTTAGTAACAAAGGCGGAGTCGGGCGCAGTGACTTCCCGCGTGGCGGTCAAAATCCACTGaggtc                                                                                                        >  2:283666/1‑136 (MQ=255)
                             aCATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCTAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATttctt                                                                                                      <  1:348022/140‑1 (MQ=255)
                             aCATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATttctt                                                                                                      <  1:348021/140‑1 (MQ=255)
                                  gCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatc                                                                                                 >  2:800053/1‑140 (MQ=255)
                                   cccTGGGCTTACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCTAAGGCGGAGTAGGGCGCTGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                <  1:33014/140‑1 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:133908/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:133836/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:133466/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                <  2:188066/140‑1 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                <  1:823078/140‑1 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                <  1:1230771/140‑1 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:1137544/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:1540401/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                <  1:1488918/140‑1 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:300081/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTAGTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAAGCAACTGATTGCTtcaagca                                                                                                >  2:817357/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTAATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTtcaatca                                                                                                >  1:624045/1‑140 (MQ=255)
                                   cccTGGGCATACGCCGGGTAATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGAGGCGAACATAATCAACTGATTTCTtcaatca                                                                                                >  1:624035/1‑140 (MQ=255)
                                        ggCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGAt                                                                                           >  1:659415/1‑140 (MQ=255)
                                        ggCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGAt                                                                                           >  1:1327988/1‑140 (MQ=255)
                                        ggCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTATTCAATCAAAGAt                                                                                           >  1:1420652/1‑140 (MQ=255)
                                             aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAAtt                                                                                      >  1:1648049/1‑140 (MQ=255)
                                             aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAAtt                                                                                      >  1:1646412/1‑140 (MQ=255)
                                             aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAAtt                                                                                      >  2:1094052/1‑140 (MQ=255)
                                             aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAAtt                                                                                      >  1:1162194/1‑140 (MQ=255)
                                             aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAAtt                                                                                      >  2:642488/1‑140 (MQ=255)
                                             aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGGAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAAtt                                                                                      >  1:995701/1‑140 (MQ=255)
                                                  gggTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAATATAAATtgctgc                                                                                 >  1:348513/1‑140 (MQ=255)
                                                  gggTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATtgctgc                                                                                 >  2:804312/1‑140 (MQ=255)
                                                  gggTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATtgctgc                                                                                 >  2:803556/1‑140 (MQ=255)
                                                   ggTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAATGATAAATTGCTGcc                                                                                >  1:77553/1‑140 (MQ=255)
                                                   ggTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGcc                                                                                >  1:797418/1‑140 (MQ=255)
                                                    gTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCa                                                                               <  1:286189/140‑1 (MQ=255)
                                                    gTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCa                                                                               <  1:670057/140‑1 (MQ=255)
                                                         gTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTggg                                                                          >  2:1202171/1‑140 (MQ=255)
                                                         gTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTggg                                                                          >  2:294188/1‑140 (MQ=255)
                                                             tgtTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGAt                                                                      >  1:1583604/1‑140 (MQ=255)
                                                             tgtTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGAGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATAAACTGATTTATTCAATCAAAGATAAAATGCTGCCACTGGGGGAt                                                                      >  1:467219/1‑140 (MQ=255)
                                                               ttttttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGa                                                                    >  1:1089130/1‑140 (MQ=255)
                                                                  tttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGt                                                                 <  2:1582317/140‑1 (MQ=255)
                                                                   ttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTg                                                                <  2:1359741/140‑1 (MQ=255)
                                                                   ttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTg                                                                <  2:1614763/140‑1 (MQ=255)
                                                                              gCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTcc                                                     <  2:1279241/140‑1 (MQ=255)
                                                                                     tgctgATTTCTGGCGATGTTAATTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCAc                                              <  2:1032532/140‑1 (MQ=255)
                                                                                       ctgATTTCTGGCGATGTTATTTTGATAGGCGGAGTAGGGCGCAGTGACTGCCCGCGTGGGGATCATAATCAACTGAGTTCTTCTAACATCGATAAATTGCTGCCACTGGGGGATGACGTGCTGTTTCTTCCGGGTCACgg                                            >  2:1241855/1‑140 (MQ=255)
                                                                                       ctgATTTCTGGCGATGTTATGTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCATTCAAAGATAAATTGCTGCCACTGGGGGAGGACGGGATATTTATTCCGGGTCACgg                                            >  2:245655/1‑140 (MQ=255)
                                                                                          aTTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACGTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGAcc                                         <  2:1162986/140‑1 (MQ=255)
                                                                                           tttCTGGCGATGTTATTTACAAAGGCGGAGTAGGGCGCAGTCACTTCCCGCGAGGCGAACATAATCAACTGATTTCTACAAACAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATACCGGGTCACGGACCa                                        <  2:619779/140‑1 (MQ=255)
                                                                                            ttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCAt                                       >  2:55355/1‑140 (MQ=255)
                                                                                                 gCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATcc                                  >  1:1195063/1‑140 (MQ=255)
                                                                                                     tGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCacac                              <  2:1162194/140‑1 (MQ=255)
                                                                                                     tGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCacac                              <  1:740830/140‑1 (MQ=255)
                                                                                                     tGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCacac                              <  1:785002/140‑1 (MQ=255)
                                                                                                      gTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGTCGTGATATTTATTCCGGGTCACGGACCTTTATCCACACt                             <  1:513820/140‑1 (MQ=255)
                                                                                                      gTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACt                             <  1:1519719/140‑1 (MQ=255)
                                                                                                              cctcggCGGAGTAGGGCGCAGTGACTTCCCCCGTGGCGAAGACTATCAACTGATTTCTTCAATCAAAGTGTAATTGCTGCCACTGGGGGATGACGTGTACTTTATTCCGGGTCACGGACCATGAGCCACACTTGGTTATg                     <  2:1529493/136‑1 (MQ=255)
                                                                                                              cacaGGCGGAGTCGGGCGCTGTGACGTCCCGCGTGGCGATCATAATCAACTGTTTTCTTCACTCAAAGATTAATTGCTGCCACTGGGGGATGACGTGCTACTGATTCCGGGTCACGGACCATGATCCACACTTGGTTATg                     <  2:1530793/137‑1 (MQ=255)
                                                                                                              cAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                     <  1:641813/140‑1 (MQ=255)
                                                                                                              cAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                     <  2:962121/140‑1 (MQ=255)
                                                                                                              cAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                     <  2:962107/140‑1 (MQ=255)
                                                                                                              cAAAGGCGGAGTAGGGCGCAGTGACTTCCAGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                     <  2:749670/140‑1 (MQ=255)
                                                                                                              cAAAGGCGGAGTAGGGCGCAGTGACTTCCAGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                     <  2:749875/140‑1 (MQ=255)
                                                                                                              cAAAGGCGGAGTAGGGCGCAGTGACTTCCAGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                     <  2:749906/140‑1 (MQ=255)
                                                                                                                   gCGGAGTAGGGCGCAGTTACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc                <  2:1423535/140‑1 (MQ=255)
                                                                                                                   gCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc                <  1:163417/140‑1 (MQ=255)
                                                                                                                   gCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc                <  1:1429516/140‑1 (MQ=255)
                                                                                                                   gCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc                <  1:1045312/140‑1 (MQ=255)
                                                                                                                   gCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTAACGGACCATTATCCACACTTGGTTATGAACGc                <  2:654886/140‑1 (MQ=255)
                                                                                                                        gTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCa           <  2:930711/140‑1 (MQ=255)
                                                                                                                           gggCTCAGTGACTTCCCGCGTGGCTATCATAATCAACTTATTTCTTCAATCATAGATAAATTGCATCCACTGGGGGTTGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATaa        <  2:72843/140‑1 (MQ=255)
                                                                                                                           gggCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCTCTGGGGGATGACGTGATATTTATTCCGGGTCTCGGACCATTATCCACACTTGGTTATGAACGCCTGCATat        <  2:974470/140‑2 (MQ=255)
                                                                                                                           gggCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATaa        <  2:974126/140‑1 (MQ=255)
                                                                                                                                 aGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCtt  >  2:1202236/1‑140 (MQ=255)
                                                                                                                                 aGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCtt  >  2:880017/1‑140 (MQ=255)
                                                                                                                                 aGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCtt  >  2:880064/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                 
AGCATAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTT  >  NC_000913/984001‑984269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑