Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,122,286	T→G	55.5%	N108H (AAC→CAC)	pyrC ←	dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,122,286	0	T	G	55.5%	45.2 / 81.1	70	N108H (AAC→CAC)	pyrC	dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base T (12/19);  new base G (15/23);  total (27/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAAT  >  NC_000913/1122167‑1122421                                                                                                                        |                                                                                                                                        atCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAg                                                                                                                     >  2:379681/1‑140 (MQ=255) atCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAg                                                                                                                     >  2:379690/1‑140 (MQ=255)   cTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCATTTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCg                                                                                                                   <  1:1653513/140‑1 (MQ=255)          tgtCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAaca                                                                                                            >  2:328161/1‑140 (MQ=255)           gtCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAacac                                                                                                           <  1:795812/140‑1 (MQ=255)           gtCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAacac                                                                                                           <  1:769696/140‑1 (MQ=255)            aaacaacacaATGCACCAGTAGCGGCAAACCGATTTTTTAAATTATCTAAAGTACCGGAAAGATTGCGACAATTGACTTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACg                                                                                                          >  1:865956/7‑140 (MQ=255)              aCTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCGATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGcc                                                                                                        >  1:158384/1‑140 (MQ=255)              aCTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGcc                                                                                                        <  2:1469961/140‑1 (MQ=255)              aCTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGcc                                                                                                        <  1:1498989/140‑1 (MQ=255)              aCTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGcc                                                                                                        <  1:958096/140‑1 (MQ=255)              aCTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGcc                                                                                                        >  1:158151/1‑140 (MQ=255)              aCTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGAGAACACGcc                                                                                                        <  1:958099/140‑1 (MQ=255)                   aCCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGt                                                                                                   >  1:193607/1‑140 (MQ=255)                   aCCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAAATGACGTCACGCCGTGGATGGAGTTAGTGGTTTCGTTTGCCGGGTAAAGTTTATCAGCGGAGAACACTCCTTctt                                                                                                   >  1:1654366/1‑138 (MQ=255)                    ccATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGtt                                                                                                  >  1:439982/1‑140 (MQ=255)                    ccATGCACCAGTAGCGGCATACCGATTTTCTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGtt                                                                                                  >  1:839953/1‑140 (MQ=255)                      aTGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTaa                                                                                                >  1:1309336/1‑140 (MQ=255)                         cACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATc                                                                                             >  2:397558/1‑140 (MQ=255)                         cACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATc                                                                                             >  1:650890/1‑140 (MQ=255)                         cACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATc                                                                                             <  1:66161/140‑1 (MQ=255)                               tAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCt                                                                                       >  1:1599120/1‑140 (MQ=255)                                cgCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  2:800109/139‑1 (MQ=255)                                aGCGGCATCCCGATGATTTCCATCCGCTCAAGTACCGGCCTGATTGCGTCACTTGACGACCCGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTATAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  2:800089/140‑1 (MQ=255)                                aGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  1:1041534/140‑1 (MQ=255)                                aGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  1:1041559/140‑1 (MQ=255)                                aGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  2:342028/140‑1 (MQ=255)                                aGCGGCATACCGATTTTTTCCATGCGCTAAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  1:108753/140‑1 (MQ=255)                                aGCGGCATACCGATTTTTTCCATGCGCTAAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  1:107302/140‑1 (MQ=255)                                aGCGGCACACCGAGTTGTACGATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTc                                                                                      <  1:1679467/140‑1 (MQ=255)                                   ggCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAg                                                                                   <  2:1117658/140‑1 (MQ=255)                                   ggCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAg                                                                                   <  2:994183/140‑1 (MQ=255)                                    gCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGc                                                                                  >  2:683139/1‑140 (MQ=255)                                     cATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCt                                                                                 <  2:865956/140‑1 (MQ=255)                                     cATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCt                                                                                 <  2:1065003/140‑1 (MQ=255)                                         ccGATTTTTTCCAAGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTATTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCAtt                                                                             <  1:979604/140‑1 (MQ=255)                                             ttttttCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGa                                                                         <  1:1280094/140‑1 (MQ=255)                                             ttttttCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGa                                                                         <  2:1434940/140‑1 (MQ=255)                                             ttttttCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCAATAGGa                                                                         <  2:1559836/140‑1 (MQ=255)                                                  tCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCCTTAGGATCCAg                                                                    >  2:877513/1‑140 (MQ=255)                                                     aTGCGCTCAAGTACCGGCAAGATAGCGTCAATTGACGTCACGCCGTGGCTGGAGATAGTGGTTGCGACTGCCGCGTAAACTATAGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCAATACGATCCAGCGa                                                                 <  2:230945/140‑1 (MQ=255)                                                      tGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGaa                                                                >  2:1500634/1‑140 (MQ=255)                                                        cgcTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATc                                                              <  1:1102126/140‑1 (MQ=255)                                                        cgcTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATc                                                              <  2:62574/140‑1 (MQ=255)                                                        cgcTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATc                                                              <  2:1092080/140‑1 (MQ=255)                                                        cgcTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTATAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATc                                                              <  2:1357367/140‑1 (MQ=255)                                                                  ccGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATaa                                                    <  1:1374299/140‑1 (MQ=255)                                                                   cGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAAc                                                   >  2:1448770/1‑140 (MQ=255)                                                                   cGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAAc                                                   <  2:85433/140‑1 (MQ=255)                                                                   cGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAAc                                                   <  2:431444/140‑1 (MQ=255)                                                                     gCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGACCACGCCTTCGTTAAATCCGCGCTCCAGCTCAGTAGGATCCAGCGAATCTGTTAAATAACAg                                                 >  1:1529147/1‑140 (MQ=255)                                                                     gCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAg                                                 <  2:1547203/140‑1 (MQ=255)                                                                     gCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAg                                                 >  1:1635214/1‑140 (MQ=255)                                                                     gCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAg                                                 >  1:334910/1‑140 (MQ=255)                                                                     gCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAg                                                 <  2:1525082/140‑1 (MQ=255)                                                                             gCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCTGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCaa                                         <  1:759676/140‑1 (MQ=255)                                                                             gCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCaa                                         <  1:160779/140‑1 (MQ=255)                                                                             gCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCaa                                         <  1:703246/140‑1 (MQ=255)                                                                              cGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAAt                                        <  2:193607/140‑1 (MQ=255)                                                                                               ccGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGccc                       <  2:1267453/140‑1 (MQ=255)                                                                                               ccGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGccc                       <  2:1267428/140‑1 (MQ=255)                                                                                                  tGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGc                    >  2:356676/1‑140 (MQ=255)                                                                                                  tGGCTGGAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGc                    >  2:1543545/1‑140 (MQ=255)                                                                                                       ggAGTTAGTGGTTGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTa               <  1:777151/140‑1 (MQ=255)                                                                                                                  ttGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGa    <  2:855907/140‑1 (MQ=255)                                                                                                                  ttGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGa    >  1:413171/1‑140 (MQ=255)                                                                                                                  ttGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACACCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGa    <  2:855920/140‑1 (MQ=255)                                                                                                                   tGCGTTTGGGGGGTATTGTTTTGCAGCGGTGAACACGCCTTCGTTATATCGGCGCTGCTGCTCATTAGGATCCAGCGAATCTGTTTTTTAACTGGTCATCAATGGGGTGTAATCGTGGCCGGCTGGTTCGGCGTcttgaa   >  2:90779/1‑135 (MQ=255)                                                                                                                   tGCGTGTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGaa   >  1:1442710/1‑140 (MQ=255)                                                                                                                    gCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAAt  >  1:1581582/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                        ATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAAT  >  NC_000913/1122167‑1122421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑