Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,174,994	G→C	5.3%	R58G (CGT→GGT)	ycfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,994	0	G	C	5.3%	269.2 / 6.2	76	R58G (CGT→GGT)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/36);  new base C (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

GCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATACATAACCCT  >  NC_000913/1174855‑1175130                                                                                                                                            |                                                                                                                                         gccagccaCTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACg                                                                                                                                          <  1:959569/140‑1 (MQ=255)    agccaCTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGaaa                                                                                                                                       >  2:1580939/1‑140 (MQ=255)      ccaCTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAgg                                                                                                                                     >  2:612135/1‑140 (MQ=255)      ccaCTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAgg                                                                                                                                     >  2:611696/1‑140 (MQ=255)      ccaCTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAgg                                                                                                                                     >  2:1298623/1‑140 (MQ=255)      ccaCTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAgg                                                                                                                                     >  1:1632999/1‑140 (MQ=255)          tGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGc                                                                                                                                 <  2:1074415/140‑1 (MQ=255)          tGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGc                                                                                                                                 <  2:929556/140‑1 (MQ=255)          tGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGc                                                                                                                                 >  1:467103/1‑140 (MQ=255)          tGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGc                                                                                                                                 >  1:1631173/1‑140 (MQ=255)               cacGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGt                                                                                                                            <  1:79253/140‑1 (MQ=255)                acGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATGTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTa                                                                                                                           <  2:1132518/140‑1 (MQ=255)                 cGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTaa                                                                                                                          >  1:476102/1‑140 (MQ=255)                    cccAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAATAAACCGGCATACGAAAGGGGGCAAGGTAAAGa                                                                                                                       >  2:717432/1‑140 (MQ=255)                    cccAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGa                                                                                                                       >  2:755676/1‑140 (MQ=255)                    cccAAAGCGCCAGCACCCAGAAGACGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGa                                                                                                                       >  2:1446927/1‑140 (MQ=255)                      cAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAATTAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGAtt                                                                                                                     >  2:1504238/1‑140 (MQ=255)                      cAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGAtt                                                                                                                     >  2:1505864/1‑140 (MQ=255)                        aaGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGa                                                                                                                   <  2:725873/140‑1 (MQ=255)                         aGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGaa                                                                                                                  >  1:364617/1‑140 (MQ=255)                         aGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGaa                                                                                                                  >  1:413092/1‑140 (MQ=255)                          gcgcCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGaaa                                                                                                                 <  1:1022780/140‑1 (MQ=255)                           cgcCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAAt                                                                                                                <  1:1152781/140‑1 (MQ=255)                                gCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGACAGGGGGCAAGGTCAAGATTGAAATAGATc                                                                                                           >  2:308733/1‑140 (MQ=255)                                 cACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATcc                                                                                                          >  2:760192/1‑140 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                <  2:1166258/140‑1 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                <  2:1207311/140‑1 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                >  2:1339975/1‑140 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                <  2:1416309/140‑1 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                >  2:1416645/1‑140 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                >  2:1585172/1‑140 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGAATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCt                                                                                                <  2:1417058/140‑1 (MQ=255)                                           aTGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGAAATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCAAACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCAAGCATTTGCt                                                                                                >  1:241040/1‑140 (MQ=255)                                             gTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCa                                                                                              >  2:450906/1‑140 (MQ=255)                                              ttCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAg                                                                                             >  1:872201/1‑140 (MQ=255)                                              ttCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAg                                                                                             >  1:876299/1‑140 (MQ=255)                                              ttCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAg                                                                                             >  1:154918/1‑140 (MQ=255)                                                    aTCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTc                                                                                       >  2:537577/1‑140 (MQ=255)                                                        gTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACCAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGAt                                                                                   >  1:760776/1‑140 (MQ=255)                                                        gTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACCAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCACAGGACTTCCGAt                                                                                   >  1:760996/1‑140 (MQ=255)                                                             tCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACgg                                                                              <  1:450906/140‑1 (MQ=255)                                                                           acACGGAACGCTGTCGATATAGCTGCGAATAAAAAAGACGGAAATAAACAAAAAAACCGGCATACGAAAGCTGTCAAGGTAAATATAGAAATAGATACACCATAGTCTCAGGAAATACGATAAAGTATGATTAAAAGTgg                                                                >  2:585384/2‑140 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGTTATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTATAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  2:1137609/140‑1 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  2:1137279/140‑1 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  1:154973/140‑1 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  1:878534/140‑1 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  1:393566/140‑1 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACCAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  2:760996/140‑1 (MQ=255)                                                                                  aCGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACCAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATg                                                         <  2:760776/140‑1 (MQ=255)                                                                                   cGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTCAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGc                                                        >  2:226611/1‑140 (MQ=255)                                                                                       gTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGAt                                                    <  2:454109/140‑1 (MQ=255)                                                                                        tCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAATCCGGCATACGAAAGGGTGCAAGGTAAAGATTTAAATAGATCCAGCATATGCTCATGACTTCCGATGACGGATGATGAAAAGTGGTCAGATGAGGATa                                                   >  2:673277/1‑140 (MQ=255)                                                                                        tCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATa                                                   >  1:1325376/1‑140 (MQ=255)                                                                                         cGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaa                                                  <  1:1432919/140‑1 (MQ=255)                                                                                         cGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaa                                                  <  1:1003709/140‑1 (MQ=255)                                                                                         cGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaa                                                  <  1:515426/140‑1 (MQ=255)                                                                                          gATATAGCGGCGAATCAAATAGCCGGATGTAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaaa                                                 <  2:264318/140‑1 (MQ=255)                                                                                          gATATAGCGGCGAATCAAATAGCCGGATATAAAGACAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaaa                                                 <  2:1184704/140‑1 (MQ=255)                                                                                          gATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaaa                                                 <  2:253901/140‑1 (MQ=255)                                                                                          gATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaaa                                                 <  2:256836/140‑1 (MQ=255)                                                                                           atatAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATaaaa                                                >  1:1139947/1‑140 (MQ=255)                                                                                                  ggcgAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATg                                         >  1:76922/1‑140 (MQ=255)                                                                                                                ccGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAAt                           >  2:810748/1‑140 (MQ=255)                                                                                                                    atatAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATcacc                       >  1:1378827/1‑140 (MQ=255)                                                                                                                      ataAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATcaccac                     <  1:1207114/140‑1 (MQ=255)                                                                                                                      ataAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATcaccac                     <  1:1235822/140‑1 (MQ=255)                                                                                                                        aaaGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCAccacca                   <  2:1036266/140‑1 (MQ=255)                                                                                                                        aaaGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCAccacca                   <  2:1035242/140‑1 (MQ=255)                                                                                                                        aaaGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCAccacca                   <  2:1034967/140‑1 (MQ=255)                                                                                                                             aaaaaaCCGGCATACGAAAGGGGGCAAGGTATAGATTGTAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACaa              <  1:1488623/140‑1 (MQ=255)                                                                                                                             aaaaaaCCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACaa              <  2:405546/140‑1 (MQ=255)                                                                                                                             aaaaaaCCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACaa              <  1:755676/140‑1 (MQ=255)                                                                                                                             aaaaaaCCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACaa              <  1:1487940/140‑1 (MQ=255)                                                                                                                              aaaaaCCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACaaa             >  1:734062/1‑140 (MQ=255)                                                                                                                               aaaaCCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAAt            >  2:1350171/1‑140 (MQ=255)                                                                                                                                         tACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATACATAACCCt  <  1:1211986/140‑1 (MQ=255)                                                                                                                                         tACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATACATAACCCt  <  1:1221045/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         GCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATACATAACCCT  >  NC_000913/1174855‑1175130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑