Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,315,945	C→A	11.4%	G31C (GGT→TGT)	yciG ←	KGG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,315,945	0	C	A	11.4%	186.7 / 7.9	64	G31C (GGT→TGT)	yciG	KGG family protein
Reads supporting (aligned to +/- strand):  ref base C (19/37);  new base A (2/6);  total (21/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTG  >  NC_000913/1315821‑1316083                                                                                                                             |                                                                                                                                           aTCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAcc                                                                                                                             <  1:1011682/140‑1 (MQ=255)  tCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCg                                                                                                                            <  2:154889/140‑1 (MQ=255)       aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCGTTTTTAAAAGTACCGCCGCTATGCTGACCGCCttt                                                                                                                       >  1:542436/1‑140 (MQ=255)       aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAc                                                                                                                              <  1:486553/133‑1 (MQ=255)       aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAc                                                                                                                              >  2:486553/1‑133 (MQ=255)            cTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGc                                                                                                                  <  2:1253215/140‑1 (MQ=255)                      taatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                                        <  1:360821/140‑1 (MQ=255)                      taatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                                        <  1:360622/140‑1 (MQ=255)                      taatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                                        >  1:29565/1‑140 (MQ=255)                        atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1618075/140‑1 (MQ=255)                        atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1101367/140‑1 (MQ=255)                        atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1193913/140‑1 (MQ=255)                        atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1101523/140‑1 (MQ=255)                         ttAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGc                                                                                                     >  1:158344/1‑140 (MQ=255)                          tAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGcc                                                                                                    <  1:128941/140‑1 (MQ=255)                                tGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCa                                                                                              <  1:1351587/140‑1 (MQ=255)                                tGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCa                                                                                              <  2:1271099/140‑1 (MQ=255)                                       ggATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCtt                                                                                       <  2:1083011/140‑1 (MQ=255)                                       ggATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCtt                                                                                       <  2:1082999/140‑1 (MQ=255)                                                  aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1158047/1‑140 (MQ=255)                                                  aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  1:1040439/1‑140 (MQ=255)                                                  aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1382482/1‑140 (MQ=255)                                                  aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1382494/1‑140 (MQ=255)                                                  aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:989637/1‑140 (MQ=255)                                                  aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATGTTTAATATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1415892/1‑140 (MQ=255)                                                      attaCCACCGCTTTGTTGCCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTg                                                                        <  1:88919/140‑1 (MQ=255)                                                      attaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGACGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTg                                                                        <  2:679662/140‑1 (MQ=255)                                                        tagcaaagCTTTGTTGACAGCCTTTTTTACACGCTTCAGATGCGAGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTTCGGCCTGCGTCGGTTGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:1640677/133‑1 (MQ=255)                                                        taCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:510036/140‑1 (MQ=255)                                                        taCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:510539/140‑1 (MQ=255)                                                        taCCACCGCTTTGTTGACCGCCTTTTGTCCCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:1401849/140‑1 (MQ=255)                                                         accaccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAac                                                                     <  1:1661139/140‑1 (MQ=255)                                                             ccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTTCCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTCCGGCGAATTTTCCTGAaccacc                                                                 <  2:1220204/140‑1 (MQ=255)                                                             ccGCTTTGTTGACCGCCTGTTTTACCCGCTTCAGATGCGCGGTGCGGATCAGTTTTAAATTTTCCGCCGATATGATGACCGCCTGTACGGGCTGCGTCGGATGCATTCTCACGGTCTTCGGCGCAATTTCGTGCacctcc                                                                 >  2:927818/1‑140 (MQ=255)                                                             ccGCTTTGTAGTCCGCCATTTTGACCCGATTCAGATGCGCGTTGCGGAACATTATTAAAATTACCGCCGCTATGCTGACCGCCTTTTCGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAaccacc                                                                 <  2:1219570/140‑1 (MQ=255)                                                                cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACg                                                              <  2:392449/140‑1 (MQ=255)                                                                cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACg                                                              <  2:556512/140‑1 (MQ=255)                                                                   tgttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATg                                                           <  2:1333255/140‑1 (MQ=255)                                                                     ttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGtt                                                         >  2:389879/1‑140 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      <  2:430355/140‑1 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      <  2:428812/140‑1 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  2:277510/1‑140 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  1:1332251/1‑140 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  1:145219/1‑140 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      <  2:1189792/140‑1 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  2:1120423/1‑140 (MQ=255)                                                                        aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  2:1120351/1‑140 (MQ=255)                                                                         ccGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGc                                                     <  1:1158047/140‑1 (MQ=255)                                                                          cGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGcc                                                    >  2:1222025/1‑140 (MQ=255)                                                                          cGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGcc                                                    >  2:173211/1‑140 (MQ=255)                                                                                         cTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                                     <  1:171000/140‑1 (MQ=255)                                                                                              gATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcgttgcg                                <  2:230295/140‑1 (MQ=255)                                                                                                    cgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCAGGTTCTTTCTCCCgttgcgttgcgt                          >  2:51120/1‑138 (MQ=255)                                                                                                        gCGGATCATTTTTAATATTACAGCCGCTATGCTGTCCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCTCGATTTTCGTCCATGTGATTTCTCCCGTTGCGTTGCATTGtt                      <  2:1189974/140‑1 (MQ=255)                                                                                                        gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:888264/140‑1 (MQ=255)                                                                                                        gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:590988/140‑1 (MQ=255)                                                                                                        gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:996932/140‑1 (MQ=255)                                                                                                        gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:10845/140‑1 (MQ=255)                                                                                                          ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCCCGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTc                    <  1:665542/140‑1 (MQ=255)                                                                                                          ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTc                    <  1:24178/140‑1 (MQ=255)                                                                                                           gATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCCCGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTct                   <  1:1448627/140‑2 (MQ=255)                                                                                                           gATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCa                   <  1:1448664/140‑1 (MQ=255)                                                                                                                 ttttAAAATTACAGCCTCTTTGCTGACCGCCTTTCCGGCCTGCGTCGGATGCCTTCTCACGGTATTCGTCGAAATTTCCTGAACCACAACTATGTTCGGCCATGTTTTTTCTCCCGTTGCGTTGCATTGTTTCATTtata             <  1:1473088/140‑1 (MQ=255)                                                                                                                      aaaTTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAgt        >  2:1560461/1‑140 (MQ=255)                                                                                                                            ccgccgCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTtgtg  <  2:158344/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                           ATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTG  >  NC_000913/1315821‑1316083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑