Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,315,945 C→A 11.4% G31C (GGT→TGT)  yciG ← KGG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,315,9450CA11.4% 186.7 / 7.9 64G31C (GGT→TGT) yciGKGG family protein
Reads supporting (aligned to +/- strand):  ref base C (19/37);  new base A (2/6);  total (21/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTG  >  NC_000913/1315821‑1316083
                                                                                                                            |                                                                                                                                          
aTCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAcc                                                                                                                             <  1:1011682/140‑1 (MQ=255)
 tCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCg                                                                                                                            <  2:154889/140‑1 (MQ=255)
      aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCGTTTTTAAAAGTACCGCCGCTATGCTGACCGCCttt                                                                                                                       >  1:542436/1‑140 (MQ=255)
      aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAc                                                                                                                              <  1:486553/133‑1 (MQ=255)
      aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAc                                                                                                                              >  2:486553/1‑133 (MQ=255)
           cTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGc                                                                                                                  <  2:1253215/140‑1 (MQ=255)
                     taatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                                        <  1:360821/140‑1 (MQ=255)
                     taatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                                        <  1:360622/140‑1 (MQ=255)
                     taatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                                        >  1:29565/1‑140 (MQ=255)
                       atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1618075/140‑1 (MQ=255)
                       atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1101367/140‑1 (MQ=255)
                       atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1193913/140‑1 (MQ=255)
                       atTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg                                                                                                      <  2:1101523/140‑1 (MQ=255)
                        ttAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGc                                                                                                     >  1:158344/1‑140 (MQ=255)
                         tAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGcc                                                                                                    <  1:128941/140‑1 (MQ=255)
                               tGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCa                                                                                              <  1:1351587/140‑1 (MQ=255)
                               tGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCa                                                                                              <  2:1271099/140‑1 (MQ=255)
                                      ggATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCtt                                                                                       <  2:1083011/140‑1 (MQ=255)
                                      ggATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCtt                                                                                       <  2:1082999/140‑1 (MQ=255)
                                                 aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1158047/1‑140 (MQ=255)
                                                 aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  1:1040439/1‑140 (MQ=255)
                                                 aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1382482/1‑140 (MQ=255)
                                                 aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1382494/1‑140 (MQ=255)
                                                 aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:989637/1‑140 (MQ=255)
                                                 aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATGTTTAATATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                            >  2:1415892/1‑140 (MQ=255)
                                                     attaCCACCGCTTTGTTGCCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTg                                                                        <  1:88919/140‑1 (MQ=255)
                                                     attaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGACGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTg                                                                        <  2:679662/140‑1 (MQ=255)
                                                       tagcaaagCTTTGTTGACAGCCTTTTTTACACGCTTCAGATGCGAGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTTCGGCCTGCGTCGGTTGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:1640677/133‑1 (MQ=255)
                                                       taCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:510036/140‑1 (MQ=255)
                                                       taCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:510539/140‑1 (MQ=255)
                                                       taCCACCGCTTTGTTGACCGCCTTTTGTCCCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGaa                                                                      <  1:1401849/140‑1 (MQ=255)
                                                        accaccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAac                                                                     <  1:1661139/140‑1 (MQ=255)
                                                            ccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTTCCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTCCGGCGAATTTTCCTGAaccacc                                                                 <  2:1220204/140‑1 (MQ=255)
                                                            ccGCTTTGTTGACCGCCTGTTTTACCCGCTTCAGATGCGCGGTGCGGATCAGTTTTAAATTTTCCGCCGATATGATGACCGCCTGTACGGGCTGCGTCGGATGCATTCTCACGGTCTTCGGCGCAATTTCGTGCacctcc                                                                 >  2:927818/1‑140 (MQ=255)
                                                            ccGCTTTGTAGTCCGCCATTTTGACCCGATTCAGATGCGCGTTGCGGAACATTATTAAAATTACCGCCGCTATGCTGACCGCCTTTTCGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAaccacc                                                                 <  2:1219570/140‑1 (MQ=255)
                                                               cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACg                                                              <  2:392449/140‑1 (MQ=255)
                                                               cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACg                                                              <  2:556512/140‑1 (MQ=255)
                                                                  tgttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATg                                                           <  2:1333255/140‑1 (MQ=255)
                                                                    ttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGtt                                                         >  2:389879/1‑140 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      <  2:430355/140‑1 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      <  2:428812/140‑1 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  2:277510/1‑140 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  1:1332251/1‑140 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  1:145219/1‑140 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      <  2:1189792/140‑1 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  2:1120423/1‑140 (MQ=255)
                                                                       aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACAGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                                      >  2:1120351/1‑140 (MQ=255)
                                                                        ccGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGc                                                     <  1:1158047/140‑1 (MQ=255)
                                                                         cGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGcc                                                    >  2:1222025/1‑140 (MQ=255)
                                                                         cGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGcc                                                    >  2:173211/1‑140 (MQ=255)
                                                                                        cTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                                     <  1:171000/140‑1 (MQ=255)
                                                                                             gATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcgttgcg                                <  2:230295/140‑1 (MQ=255)
                                                                                                   cgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCAGGTTCTTTCTCCCgttgcgttgcgt                          >  2:51120/1‑138 (MQ=255)
                                                                                                       gCGGATCATTTTTAATATTACAGCCGCTATGCTGTCCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCTCGATTTTCGTCCATGTGATTTCTCCCGTTGCGTTGCATTGtt                      <  2:1189974/140‑1 (MQ=255)
                                                                                                       gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:888264/140‑1 (MQ=255)
                                                                                                       gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:590988/140‑1 (MQ=255)
                                                                                                       gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:996932/140‑1 (MQ=255)
                                                                                                       gCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGtt                      <  2:10845/140‑1 (MQ=255)
                                                                                                         ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCCCGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTc                    <  1:665542/140‑1 (MQ=255)
                                                                                                         ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTc                    <  1:24178/140‑1 (MQ=255)
                                                                                                          gATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCCCGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTct                   <  1:1448627/140‑2 (MQ=255)
                                                                                                          gATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCa                   <  1:1448664/140‑1 (MQ=255)
                                                                                                                ttttAAAATTACAGCCTCTTTGCTGACCGCCTTTCCGGCCTGCGTCGGATGCCTTCTCACGGTATTCGTCGAAATTTCCTGAACCACAACTATGTTCGGCCATGTTTTTTCTCCCGTTGCGTTGCATTGTTTCATTtata             <  1:1473088/140‑1 (MQ=255)
                                                                                                                     aaaTTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAgt        >  2:1560461/1‑140 (MQ=255)
                                                                                                                           ccgccgCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTtgtg  <  2:158344/140‑1 (MQ=255)
                                                                                                                            |                                                                                                                                          
ATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTG  >  NC_000913/1315821‑1316083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: