Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,355,374 C→A 6.2% A33S (GCG→TCG)  sapB ← antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,355,3740CA 6.2% 215.1 / 2.2 65A33S (GCG→TCG) sapBantimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (33/28);  new base A (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAGCAA  >  NC_000913/1355239‑1355513
                                                                                                                                       |                                                                                                                                           
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  1:1144328/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  1:1144400/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  1:11598/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  2:1265678/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  1:698348/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  1:698318/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  2:477296/1‑140 (MQ=255)
tcgtcgCCGGGAAGACCTCTTTGAACTGGTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCCAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGa                                                                                                                                         >  1:658210/1‑140 (MQ=255)
 cgtcgCCGGGAAGACCTCTTTTCACTGTTCGGCAATCGGTTGAACGTTAATGCTGGTAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCCGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAg                                                                                                                                        <  2:1208258/140‑1 (MQ=255)
   tcgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGTCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAggg                                                                                                                                      >  1:396068/1‑140 (MQ=255)
    cgCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAgggg                                                                                                                                     <  2:97133/140‑1 (MQ=255)
        gggAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGaa                                                                                                                                 <  2:1473438/140‑1 (MQ=255)
                       aaCTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGaa                                                                                                                  <  1:387907/140‑1 (MQ=255)
                        aCTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCACAAGCTGAAg                                                                                                                 >  2:1480813/1‑140 (MQ=255)
                           gTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCATGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCa                                                                                                              >  1:1186974/1‑140 (MQ=255)
                             tCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAAc                                                                                                            >  2:419806/1‑140 (MQ=255)
                             tCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAAc                                                                                                            >  2:31247/1‑140 (MQ=255)
                             tCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAAc                                                                                                            >  2:861484/1‑140 (MQ=255)
                                      ggTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATAACACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATATCTCAAGCTGAAGCCAACGAAGGTcag                                                                                                   >  1:524201/1‑140 (MQ=255)
                                      ggTTGACCGTTAATGCTGGAAACAACAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTcag                                                                                                   >  1:1637883/1‑140 (MQ=255)
                                       gTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTcagc                                                                                                  <  1:806370/140‑1 (MQ=255)
                                            ccGTTAATGCTGGAAACACCACAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCAGTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGaa                                                                                             <  2:161777/140‑1 (MQ=255)
                                            ccGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGaa                                                                                             <  2:159500/140‑1 (MQ=255)
                                             cGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAAc                                                                                            >  1:999383/1‑140 (MQ=255)
                                                  aTGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGAATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGt                                                                                       >  1:46501/1‑140 (MQ=255)
                                                  aTGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGAATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGt                                                                                       >  1:46500/1‑140 (MQ=255)
                                                    gCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGa                                                                                     <  2:1416472/140‑1 (MQ=255)
                                                    gCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGa                                                                                     <  2:975757/140‑1 (MQ=255)
                                                      tGGAAACACCAACATCCCAGAGGATCAGGCCGTTAAACCACAACACCCCCGCCTACCACCGTGACTCGCCATGTAACTGCGCATGAGGCGCGAAATAGCTCAAGCTGAAGCCAACTAAGGTCAGCAGGAAAAAGCTGAca                                                                                   <  2:1104032/140‑1 (MQ=255)
                                                      tGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGAca                                                                                   <  2:807251/140‑1 (MQ=255)
                                                      tGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGAca                                                                                   <  1:221364/140‑1 (MQ=255)
                                                      aggAAACCCCAAAATCCCCGTGGAACAGGCCGTCAAACCACACCACCCCGTCATTCCACAGTGACGCGCCCTGTAACGGCGCACGAGGGGTGACACACCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACACGGTGAcc                                                                                   <  2:998884/139‑2 (MQ=255)
                                                                    tCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAAt                                                                     <  2:384684/140‑1 (MQ=255)
                                                                       cAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATgcg                                                                  >  2:139938/1‑140 (MQ=255)
                                                                         gTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATgcggc                                                                >  2:1060588/1‑140 (MQ=255)
                                                                         gTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATgcggc                                                                >  1:642469/1‑140 (MQ=255)
                                                                          tGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATgcggcg                                                               <  2:373809/140‑1 (MQ=255)
                                                                           ggATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGATATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGt                                                              <  2:120111/140‑1 (MQ=255)
                                                                                      ttAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGAt                                                   >  1:1153017/1‑140 (MQ=255)
                                                                                      ttAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGAt                                                   >  2:1559630/1‑140 (MQ=255)
                                                                                         aaCCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAAt                                                <  1:1096717/140‑1 (MQ=255)
                                                                                           ccAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCa                                              >  1:1365355/1‑140 (MQ=255)
                                                                                             aGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATg                                            <  2:1101988/140‑1 (MQ=255)
                                                                                             aGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATg                                            <  2:1168914/140‑1 (MQ=255)
                                                                                                    ccAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGtttttt                                     >  2:174934/1‑140 (MQ=255)
                                                                                                     cAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTc                                    <  1:176557/140‑1 (MQ=255)
                                                                                                     cAGGCATTCCACAGTGACGCGCCTTGTAACGGCGAATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTc                                    <  2:46501/140‑1 (MQ=255)
                                                                                                     cAGGCATTCCACAGTGACGCGCCTTGTAACGGCGAATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTc                                    <  2:46500/140‑1 (MQ=255)
                                                                                                      aGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCa                                   >  2:263990/1‑140 (MQ=255)
                                                                                                        gCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCAcc                                 <  2:1622974/140‑1 (MQ=255)
                                                                                                           ttCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACTAAGGTCAGCAGGTACAAGGTGGCAATCAATAACAAAATGCGGCGGAAGGGGATGATAATCATGGTTTTTTCACCTCa                              >  2:606928/1‑140 (MQ=255)
                                                                                                            tCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCTGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCAt                             <  1:941277/140‑1 (MQ=255)
                                                                                                            tCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCAt                             >  1:631973/1‑140 (MQ=255)
                                                                                                            tCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTATTCACCTCAt                             >  1:145457/1‑140 (MQ=255)
                                                                                                                  gTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGtt                       <  1:1406071/140‑1 (MQ=255)
                                                                                                                   tGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGttt                      >  1:1421232/1‑140 (MQ=255)
                                                                                                                     aCGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTtct                    <  1:1367125/140‑1 (MQ=255)
                                                                                                                      cgcgCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTtctc                   >  1:216621/1‑140 (MQ=255)
                                                                                                                      cgcgCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTtctc                   >  1:625732/1‑140 (MQ=255)
                                                                                                                       gcgcCTTGTACCGGCGCATGATGCGTGAAATAGCTCAAGCTGAAGCCAACGAACGTCAGCAGGAACATGGTGAAAATCAATAACAAAATGCGGAGTAAGGTGAAGAAAATCATGGTTTTTTCACCTCATCCTGTTTCTcg                  <  2:1681590/140‑1 (MQ=255)
                                                                                                                       gcgcCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGATGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCTGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTcg                  <  2:1198281/140‑1 (MQ=255)
                                                                                                                       gcgcCATGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTGATCCTGTTTCTcg                  <  2:350234/140‑1 (MQ=255)
                                                                                                                        cgcCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTcgc                 >  2:673832/1‑140 (MQ=255)
                                                                                                                                  cGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACAcccc       <  2:1604523/140‑1 (MQ=255)
                                                                                                                                  cGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACAcccc       >  1:641517/1‑140 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAATCCAACGAAGGTCAGCAGGATCAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAg     <  2:580432/140‑1 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACATAATGCGGCGTAAGGTGAAGATAATCTTGGTTTTTTCACCTCTTCCTGTTTCTCGCGATACACCCCAg     <  2:189994/140‑1 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAg     <  2:972322/140‑1 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAg     <  2:972303/140‑1 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAg     <  2:1010510/140‑1 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAg     <  2:1298491/140‑1 (MQ=255)
                                                                                                                                    gcgcATGAGGGGTGAAATAGCTCAAGCTGAAGACAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAg     <  2:1605842/140‑1 (MQ=255)
                                                                                                                                     cgcATGAGGGGTGAAATAGCTCAAGCAGAAGCCAACGAAGGTCCGCTGGAACACGGTGACAAACATTAACAAAATGCTGCGGAAGGTGAAGAAAATCATGGTGTGTACAAGTCATCCTCGTTCTCGCGAGACACCCCAGc    >  2:1671218/1‑140 (MQ=255)
                                                                                                                                       cATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAGCaa  >  2:120183/1‑140 (MQ=255)
                                                                                                                                       cATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAGCaa  >  2:120160/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
TCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACGCGCCTTGTAACGGCGCATGAGGGGTGAAATAGCTCAAGCTGAAGCCAACGAAGGTCAGCAGGAACAAGGTGACAATCAATAACAAAATGCGGCGTAAGGTGAAGATAATCATGGTTTTTTCACCTCATCCTGTTTCTCGCGATACACCCCAGCAA  >  NC_000913/1355239‑1355513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: