Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,889,637 G→A 5.6% A37V (GCC→GTC)  fadD ← acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,889,6370GA 5.6% 248.6 / 3.9 71A37V (GCC→GTC) fadDacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base G (37/30);  new base A (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TAGGCATCATCAACGCAACGCGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTC  >  NC_000913/1889500‑1889774
                                                                                                                                         |                                                                                                                                         
tAGGCATCATCAACGCAACGCGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCg                                                                                                                                         <  1:1455427/140‑1 (MQ=255)
  ggCATCATCAACGCAACGCGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTa                                                                                                                                       >  2:1421259/1‑140 (MQ=255)
  ggCATCATCAACGCAACGCGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTa                                                                                                                                       >  2:1420611/1‑140 (MQ=255)
              gcaacgcGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGcgaccga                                                                                                                           <  1:928755/140‑1 (MQ=255)
              gcaacgcGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGcgaccga                                                                                                                           <  1:1246708/140‑1 (MQ=255)
              gcaacgcGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGcgaccga                                                                                                                           <  2:83551/140‑1 (MQ=255)
                 acgcGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTg                                                                                                                        <  2:1499778/140‑1 (MQ=255)
                                        cAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACGGCGTTCTTCCAGCTTGCGGAAGGGCGTTACCTCCCCCAGCTGCTCCATCTCAGGGAGATCGGCGTAGCGCGCGTCCGTCTGCTCGATCTTATCGACCAgagagt                                                                                                 >  2:740309/1‑138 (MQ=255)
                                            ccTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATa                                                                                             <  1:20576/140‑1 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCGGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACg                                                                                          >  2:1178564/1‑140 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGGTAACg                                                                                          >  2:584400/1‑140 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACg                                                                                          >  1:952662/1‑140 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACg                                                                                          >  2:422922/1‑140 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACg                                                                                          >  2:572247/1‑140 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACg                                                                                          >  2:584127/1‑140 (MQ=255)
                                               tgttgCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACAAGAGATTGATAACg                                                                                          >  1:459903/1‑140 (MQ=255)
                                                      aaTAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAggg                                                                                   >  1:541772/1‑140 (MQ=255)
                                                      aaTAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAggg                                                                                   >  2:699647/1‑140 (MQ=255)
                                                      aaTAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAggg                                                                                   >  2:797014/1‑140 (MQ=255)
                                                      aaTAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAggg                                                                                   >  2:895605/1‑140 (MQ=255)
                                                           gCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAt                                                                              <  1:498753/140‑1 (MQ=255)
                                                           gCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAt                                                                              <  2:813971/140‑1 (MQ=255)
                                                           gCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAt                                                                              <  1:1073616/140‑1 (MQ=255)
                                                                            gCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCcg                                                             <  1:173098/140‑1 (MQ=255)
                                                                            gCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCcg                                                             <  1:685943/140‑1 (MQ=255)
                                                                            gCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCcg                                                             <  2:483488/140‑1 (MQ=255)
                                                                              gTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCcgcg                                                           <  1:1551302/140‑1 (MQ=255)
                                                                                  ttcCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGAt                                                       <  1:907971/140‑1 (MQ=255)
                                                                                  ttcCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACAAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGAt                                                       <  2:459903/140‑1 (MQ=255)
                                                                                        cTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGt                                                 <  2:1018458/140‑1 (MQ=255)
                                                                                            cGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAg                                             >  2:824313/1‑140 (MQ=255)
                                                                                            cGGAAGGTCATTACCTCCCCCAAATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAg                                             >  2:90946/1‑140 (MQ=255)
                                                                                              gAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGcc                                           <  1:407291/140‑1 (MQ=255)
                                                                                              gAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGcc                                           <  1:1384198/140‑1 (MQ=255)
                                                                                              gAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGcc                                           <  2:902520/140‑1 (MQ=255)
                                                                                              gAAGGTCATTACCACCCCCATAATCACAAACCCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATAACTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGcc                                           <  2:1148971/140‑1 (MQ=255)
                                                                                                 ggTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCCGCCTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCaaa                                        <  1:705760/140‑1 (MQ=255)
                                                                                                   tCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAAcc                                      <  1:881520/140‑1 (MQ=255)
                                                                                                   tCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAAcc                                      <  2:147258/140‑1 (MQ=255)
                                                                                                      ttACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACcttc                                   >  1:1008508/1‑140 (MQ=255)
                                                                                                      ttACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACcttc                                   >  1:1008285/1‑140 (MQ=255)
                                                                                                        aCCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACcttctt                                 <  2:1140673/140‑1 (MQ=255)
                                                                                                         ccTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCttcttc                                <  1:572247/140‑1 (MQ=255)
                                                                                                            cccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAt                             >  2:1277523/1‑140 (MQ=255)
                                                                                                            cccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAt                             >  2:1577575/1‑140 (MQ=255)
                                                                                                            cccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAt                             >  2:509405/1‑140 (MQ=255)
                                                                                                            cccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGACAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAt                             >  2:985265/1‑140 (MQ=255)
                                                                                                             ccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCATAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAtt                            >  1:1654723/1‑140 (MQ=255)
                                                                                                             ccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAtt                            >  1:27545/1‑140 (MQ=255)
                                                                                                             ccccATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAtt                            >  2:709922/1‑140 (MQ=255)
                                                                                                                cATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCTAACCTTCTTCAAttctt                         <  2:981164/140‑1 (MQ=255)
                                                                                                                cATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         <  1:1115012/140‑1 (MQ=255)
                                                                                                                cATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         <  2:143401/140‑1 (MQ=255)
                                                                                                                cATATTAACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         <  1:656947/140‑1 (MQ=255)
                                                                                                                    ttCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCAcc                     <  1:1539482/140‑1 (MQ=255)
                                                                                                                       acaAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACctct                  >  2:546643/1‑140 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTaa                >  2:968848/1‑140 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTaa                >  2:976077/1‑140 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTaa                >  2:1431116/1‑140 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGACGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         >  1:577358/1‑131 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGACGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         >  1:577337/1‑131 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGACGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         <  2:577358/131‑1 (MQ=255)
                                                                                                                         aaaCGCAGGTTGATCGACGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAAttctt                         <  2:577337/131‑1 (MQ=255)
                                                                                                                              cAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATGTACCAGAGATTGTTAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCATAGGTTCTTCATTTCTTCAGCTCTAAATTgg           >  2:405848/1‑139 (MQ=255)
                                                                                                                              cAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGc           >  2:1470587/1‑140 (MQ=255)
                                                                                                                                 gTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCgtg        >  1:747020/1‑140 (MQ=255)
                                                                                                                                 gTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCgtg        >  1:747030/1‑140 (MQ=255)
                                                                                                                                   tGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGtt      >  2:564202/1‑140 (MQ=255)
                                                                                                                                     aTCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTtcg    >  1:518385/1‑140 (MQ=255)
                                                                                                                                       cGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTtcgtc  >  1:1571759/1‑140 (MQ=255)
                                                                                                                                       cGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTtcgtc  >  1:1010642/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TAGGCATCATCAACGCAACGCGATCGCCTTTCTTCAGCCCCAACCCTTGTTGCAAATAAGCGGCAAACGCGCGACTGCGTTCTTCCAGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGGAACGTCCGCGGGATAACGGTTAAGCCAAACCTTCTTCAATTCTTCACCTCTAAAATGCGTGTTCGTC  >  NC_000913/1889500‑1889774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: