| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,959,898 | C→T | 9.1% | intergenic (‑52/‑164) | yecM ← / → argS | putative metal‑binding enzyme/arginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,959,898 | 0 | C | T | 9.1% | 138.9 / 4.5 | 44 | intergenic (‑52/‑164) | yecM/argS | putative metal‑binding enzyme/arginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (19/21); new base T (2/2); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CGGGAAAGTTCATCTAATGCGTGGATAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGCT > NC_000913/1959761‑1960036 | cggGAAAGTTCATCTAATGCGTGGATAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGcca > 1:369613/1‑140 (MQ=255) aaGTTCATCTAATGCGTGGACAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGccaaccaa < 1:918623/140‑1 (MQ=255) tCATCTAATGCGTGGATAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGc < 2:488627/140‑1 (MQ=255) tGGATAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCAcgcc > 2:1254708/1‑140 (MQ=255) aTAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCt < 2:77824/140‑1 (MQ=255) ataTCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTc < 2:265791/138‑1 (MQ=255) aaaTCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTc < 2:265725/140‑1 (MQ=255) aaaTCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTc < 2:776588/140‑1 (MQ=255) aTCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTg < 1:287053/140‑1 (MQ=255) tCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGGAAAAGCGGTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGa > 1:1465562/1‑140 (MQ=255) ggTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGTCAACCAACCGCTGATTTCACGCCGCTTCTGATGCa > 1:878361/1‑140 (MQ=255) ggTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGTCAACCAACCGCTGATTTCACGCCGCTTCTGATGCa > 1:878340/1‑140 (MQ=255) aTGCAATATCCTGCAGCTCGTCGTTAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGaaaa < 1:1458190/140‑1 (MQ=255) aTGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGaaaa < 1:1148311/140‑1 (MQ=255) tGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGc > 2:615529/1‑140 (MQ=255) cgtcgATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACgcgc > 1:751417/1‑140 (MQ=255) gtcgATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCa < 2:373534/140‑1 (MQ=255) gtcgAAAGATTGCCAGTTCGCCATAATCACACTTCGTCTTTCAGTAAAAGCGTTAATATACCCTCTTGCCCTGTGCCAAACAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCa < 2:781467/140‑1 (MQ=255) tcgATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATATTGAAAACGGAAATACGCCACGCGCAc > 2:1661375/1‑140 (MQ=255) cgATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACg > 1:170758/1‑140 (MQ=255) aTAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCAt < 2:567710/140‑1 (MQ=255) aTAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCAt < 1:1198874/140‑1 (MQ=255) ctCACTCTTCGTCTTTCAGTAAACGCGTGCATGGTCCCTGGTGCCCTGGGCCCACCACCCGCTGATTCCCCGCCGCTTCTGAGGCTAGCGTGAAAACGGCAAGACGCCACGCGCACGTTGCTGACGAAAACAGCCAtttt < 2:1248668/139‑2 (MQ=255) aTCACTCTTCGTCTTTCATTAAAAGAGTTAATTTACCCTGTTTCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGTTGCAATTGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg < 2:395725/140‑1 (MQ=255) aTCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAATCGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg < 1:1448882/140‑1 (MQ=255) aTCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg < 2:369613/140‑1 (MQ=255) aTCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg < 2:19895/140‑1 (MQ=255) aTCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg < 2:265418/140‑1 (MQ=255) cACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGTCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCa < 2:878361/140‑1 (MQ=255) cACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGTCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCa < 2:878340/140‑1 (MQ=255) gTCTTTAAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg < 1:728270/130‑1 (MQ=255) gTCTTTAAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTg > 2:728270/1‑130 (MQ=255) cTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTccc < 2:742153/140‑1 (MQ=255) aGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCt > 2:1240272/1‑140 (MQ=255) tAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTaa > 2:1565731/1‑140 (MQ=255) aaGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAAttt > 2:1037363/1‑140 (MQ=255) aaGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAAttt > 2:1037356/1‑140 (MQ=255) aaGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAAttt > 2:1075264/1‑140 (MQ=255) ttGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCa > 2:1031887/1‑140 (MQ=255) tgCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACTGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCTGTATACTCCCGCCCTAATTTCTTAAACTGGTGCGGGCAATTTTTGc > 1:1472245/1‑140 (MQ=255) tgCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTgt > 1:408410/1‑139 (MQ=255) tgCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGc > 1:497192/1‑140 (MQ=255) tgCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGc > 1:1631583/1‑140 (MQ=255) tgCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGc > 2:147969/1‑140 (MQ=255) tgCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGc > 2:147546/1‑140 (MQ=255) gCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGCt < 2:739462/140‑1 (MQ=255) | CGGGAAAGTTCATCTAATGCGTGGATAAATCGCGGTAAATCCGATGCAATATCCTGCAGCTCGTCGATAGATTGCCAGTTCGCCATAATCACTCTTCGTCTTTCAGTAAAAGCGTTAATTTACCCTGTTGCCCTGTGCCAACCAACCGCTGATTTCACGCCGCTTCTGATGCAATAGTGAAAACGGCAATACGCCACGCGCACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAATTTTTGCT > NC_000913/1959761‑1960036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |