Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,059,824	G→T	7.6%	intergenic (‑134/‑27)	yeeO ← / → asnU	putative multdrug exporter, MATE family/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,824	0	G	T	7.6%	227.5 / 2.7	67	intergenic (‑134/‑27)	yeeO/asnU	putative multdrug exporter, MATE family/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base G (16/45);  new base T (3/3);  total (19/48)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACATTTCAAATAATGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATT  >  NC_000913/2059685‑2059959                                                                                                                                            |                                                                                                                                        ccACATTTCAAATAATGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGTGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCg                                                                                                                                         <  2:1077741/140‑1 (MQ=255) ccACATTTCAAATAATGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCg                                                                                                                                         <  2:901327/140‑1 (MQ=255) ccACATTTAAAATAATGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACACATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCg                                                                                                                                         <  1:18611/140‑1 (MQ=255) ccACATTTAAAATAATGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCg                                                                                                                                         <  1:1269340/140‑1 (MQ=255)            ataatGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGAtg                                                                                                                              <  2:1455426/140‑1 (MQ=255)            ataatGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGAtg                                                                                                                              <  2:1455649/140‑1 (MQ=255)              aatGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGAtgtg                                                                                                                            <  2:1432632/140‑1 (MQ=255)               atGAGGGAGAATCAGCATAGCTGCAAAGATTTTCGCCAACAAATTGTCTTCAAATGCAACAAAATGTTGATAGAAACGGCAAACACTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGc                                                                                                                           <  2:784047/140‑1 (MQ=255)               atGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGc                                                                                                                           >  2:929484/1‑140 (MQ=255)               atGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGc                                                                                                                           <  2:585797/140‑1 (MQ=255)               atGAGGGAGAATCAGAAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATATAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGc                                                                                                                           <  1:976400/140‑1 (MQ=255)                     gagaATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTcaac                                                                                                                     <  1:1536646/140‑1 (MQ=255)                     gagaATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTcaac                                                                                                                     <  1:1536629/140‑1 (MQ=255)                        aaTCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCaacaac                                                                                                                  >  2:998463/1‑140 (MQ=255)                        aaTCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCaacaac                                                                                                                  >  1:502678/1‑140 (MQ=255)                          tCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGa                                                                                                                <  1:1385514/140‑1 (MQ=255)                             tcAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTc                                                                                                             <  2:757703/139‑1 (MQ=255)                             gCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTc                                                                                                             <  2:472694/140‑1 (MQ=255)                               aaaGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCct                                                                                                           <  2:1337981/140‑1 (MQ=255)                                  gCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTg                                                                                                        >  1:1595928/1‑140 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  1:139104/140‑1 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  2:1255306/140‑1 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  2:1255314/140‑1 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  2:1583965/140‑1 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  2:587916/140‑1 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  1:139133/140‑1 (MQ=255)                                         aGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCa                                                                                                 <  2:588001/140‑1 (MQ=255)                                                  ccAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGa                                                                                        <  1:553885/140‑1 (MQ=255)                                                        aaTTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAAcggcg                                                                                  <  2:1003901/140‑1 (MQ=255)                                                        aaTTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGATGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCTCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAAcggcg                                                                                  <  2:1297044/140‑1 (MQ=255)                                                        aaTTGTCTGCAAAAGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTGTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAAcggcg                                                                                  <  2:1471008/140‑1 (MQ=255)                                                          ttctCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGa                                                                                <  1:957451/137‑1 (MQ=255)                                                          ttGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGa                                                                                <  2:1234250/140‑1 (MQ=255)                                                          ttGACTTCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATATAAAAATCGGGTTAGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGa                                                                                <  1:1320212/140‑1 (MQ=255)                                                               tGCAAATGCAACAAACTGTTGATAGAAACGGCAAACTGTTGGGGAATTTAAAATTCGGGTTTGACAAATGTTTTTTCGCCGTTAAGATGTTCCTCATCAACGATTCCTCTGTAGTTCAGTCGGTATAACGGCGGTGTGtt                                                                           >  2:906915/1‑140 (MQ=255)                                                                  aaaTGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAAt                                                                        <  2:215189/140‑1 (MQ=255)                                                                  aaaTGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAAt                                                                        <  2:215222/140‑1 (MQ=255)                                                                  aaaTGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAAt                                                                        <  1:1020641/140‑1 (MQ=255)                                                                  aaaTGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAAt                                                                        <  1:1020588/140‑1 (MQ=255)                                                                     tGCAACATACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAATAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGCTGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCg                                                                     <  2:619020/140‑1 (MQ=255)                                                                       caacaaACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTa                                                                   <  1:1126442/140‑1 (MQ=255)                                                                       caacaaACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTa                                                                   >  1:249581/1‑140 (MQ=255)                                                                       caacaaACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAAACGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGGTAATGCGTa                                                                   >  2:1662343/1‑140 (MQ=255)                                                                            aaCTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCa                                                              <  1:630686/140‑1 (MQ=255)                                                                             aCTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTTACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCAc                                                             <  1:1105164/140‑1 (MQ=255)                                                                                    aTAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCg                                                      >  1:241593/1‑140 (MQ=255)                                                                                    aTAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTATTCTCCGTTAAGATGTGCCTCAACAACGATTCCACAGTAGTTCAGTCGGTAGAACGGCGGAATGTTAATCCGAATGTCAcatgatcg                                                      >  1:241542/1‑133 (MQ=37)                                                                                      aGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGAGTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAg                                                    <  1:1105261/140‑1 (MQ=255)                                                                                       gAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGt                                                   <  1:559997/140‑1 (MQ=255)                                                                                       gAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGt                                                   <  2:422346/140‑1 (MQ=255)                                                                                                    gTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAgaggag                                      <  1:402520/140‑1 (MQ=255)                                                                                                       ggggAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCa                                   <  1:1035729/140‑1 (MQ=255)                                                                                                       ggggAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCa                                   <  1:756218/140‑1 (MQ=255)                                                                                                        gggAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCaa                                  >  2:1025479/1‑140 (MQ=255)                                                                                                            aTTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAttt                              >  2:189673/1‑139 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTa                                                                          >  1:802886/1‑95 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTa                                                                          <  2:802886/95‑1 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTc                                                               >  2:165395/1‑106 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTc                                                               <  1:165395/106‑1 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAtttt                             >  2:931998/1‑138 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAACATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAtttt                             >  2:288337/1‑138 (MQ=255)                                                                                                             tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTAAAGATGTGCCTCAACAACGATTCCACTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAATcc                             >  2:1179061/1‑140 (MQ=255)                                                                                                             tatAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTcc                             >  1:1147231/3‑140 (MQ=255)                                                                                                               tAAAAATCGGGTTTGACAAAAGATTTTTCTCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAgaggag                                      >  2:1351385/1‑129 (MQ=255)                                                                                                               tAAAAATCGGGTTTGACAAAAGATTTTTCTCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAgaggag                                      >  2:1351261/1‑129 (MQ=255)                                                                                                               tAAAAATCGGGTTTGACAAAAGATTTTTCTCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAgaggag                                      <  1:1351261/129‑1 (MQ=255)                                                                                                               tAAAAATCGGGTTTGACAAAAGATTTTTCTCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAgaggag                                      <  1:1351385/129‑1 (MQ=255)                                                                                                                      cGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGcc                    <  1:1150360/140‑1 (MQ=255)                                                                                                                      cGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGcc                    <  1:551624/140‑1 (MQ=255)                                                                                                                                  aaGATTCTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGc        <  2:1330172/140‑1 (MQ=255)                                                                                                                                        tttCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAtt  <  1:798534/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        CCACATTTCAAATAATGAGGGAGAATCAGCAAAGCTGCAAAGATTTTCGCCAACAAATTGTCTGCAAATGCAACAAACTGTTGATAGAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATT  >  NC_000913/2059685‑2059959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑