Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,109,488 G→T 8.2% L33I (CTT→ATT)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,109,4880GT 8.2% 163.3 / 2.2 55L33I (CTT→ATT) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base G (21/29);  new base T (3/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAG  >  NC_000913/2109363‑2109623
                                                                                                                             |                                                                                                                                       
tgctTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTaa                                                                                                                           >  2:1164925/1‑140 (MQ=255)
   tttGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCa                                                                                                                        <  2:167404/140‑1 (MQ=255)
         aaTTCTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTa                                                                                                                  <  1:158764/140‑1 (MQ=255)
               ctGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGAt                                                                                                            <  2:1047096/140‑1 (MQ=255)
                     tGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGc                                                                                                      <  2:820112/140‑1 (MQ=255)
                     tGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGc                                                                                                      >  2:422643/1‑140 (MQ=255)
                     tGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGc                                                                                                      <  2:1515927/140‑1 (MQ=255)
                     tGCTGTGAGATTGAAACCATATTCAACAAAAATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGc                                                                                                      <  2:1533366/140‑1 (MQ=255)
                       cTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTa                                                                                                    >  2:1416333/1‑140 (MQ=255)
                       cTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTa                                                                                                    >  2:1365900/1‑140 (MQ=255)
                                    aCCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAg                                                                                       >  2:1033898/1‑140 (MQ=255)
                                       atatTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCa                                                                                    <  2:1378779/140‑1 (MQ=255)
                                        tatTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAg                                                                                   >  1:960159/1‑140 (MQ=255)
                                        tatTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAg                                                                                   >  1:960169/1‑140 (MQ=255)
                                        tagtCAACAAACATTATCATATATAGCATCGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAg                                                                                   <  2:1442066/137‑1 (MQ=255)
                                                              ataGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTaaa                                                             <  1:392976/140‑1 (MQ=255)
                                                              ataGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTaaa                                                             >  1:722632/1‑140 (MQ=255)
                                                              ataGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTaaa                                                             >  1:974317/1‑140 (MQ=255)
                                                                aGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCGGATATATAACGTTTCTTCTTAAAGa                                                           >  2:917374/1‑140 (MQ=255)
                                                                aGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGa                                                           >  2:917896/1‑140 (MQ=255)
                                                                  cATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATa                                                         >  1:2519/1‑140 (MQ=255)
                                                                  cATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATa                                                         >  2:773309/1‑140 (MQ=255)
                                                                       tCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATGTAACGTTTCTTCTTAAAGATCATTTa                                                    >  2:1662012/1‑140 (MQ=255)
                                                                       tCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTa                                                    >  1:408570/1‑140 (MQ=255)
                                                                       tCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTa                                                    >  2:167064/1‑140 (MQ=255)
                                                                        cGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTAt                                                   <  2:1585053/140‑1 (MQ=255)
                                                                            tGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGt                                               >  1:959816/1‑140 (MQ=255)
                                                                            tGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGt                                               >  2:149673/1‑140 (MQ=255)
                                                                            tGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGt                                               >  2:147940/1‑140 (MQ=255)
                                                                                   aaCCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATg                                        <  1:948251/140‑1 (MQ=255)
                                                                                        aaTATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCaatt                                   >  1:818532/1‑140 (MQ=255)
                                                                                               cgatcgtttCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCACTGGTAAATGATAATTGCTACCATGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCTATTATTTTTa                            <  2:1386925/134‑1 (MQ=255)
                                                                                               cGAAATTTTCAGTAAAAATTTTTCTTACTATATATTTAAATGTAATCAATTGTAAAAGATAATTGATACATTGAACGACATCCATATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTAATTCAAttttttttt                            <  1:123282/140‑2 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATTGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:343823/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAACAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:710746/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:686484/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:671798/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  1:1382171/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:897233/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  1:1165177/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  1:1121723/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:1531908/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:1522561/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:1382816/140‑1 (MQ=255)
                                                                                               cGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  1:436926/140‑1 (MQ=255)
                                                                                               cGAAATTGTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTa                            <  2:1191036/140‑1 (MQ=255)
                                                                                                 aaaTTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAAt                          <  1:395844/140‑1 (MQ=255)
                                                                                                              cAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCAt             <  1:1412550/140‑1 (MQ=255)
                                                                                                                aGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCt           >  1:259853/1‑140 (MQ=255)
                                                                                                                  tgtTCTTACAATATAAGCAAATGTAAGCAATGTTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTaa         <  2:507492/140‑1 (MQ=255)
                                                                                                                  tgtTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTaa         <  2:1478356/140‑1 (MQ=255)
                                                                                                                  tgtTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTaa         <  2:1478254/140‑1 (MQ=255)
                                                                                                                         aCAATATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAag  >  1:31073/1‑140 (MQ=255)
                                                                                                                         aCAATATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAag  >  1:31284/1‑140 (MQ=255)
                                                                                                                         aCAATATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAAGTAATTTTAATCTGATAAGCTCATCTAACGTAAag  >  1:31285/1‑140 (MQ=255)
                                                                                                                             |                                                                                                                                       
TGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATATACCGAAATTTTCAGGACCAAGTGTTCTTACAAGATATGGAAATGTAAGCAATGGTAAAAGATAATTGCTACCTTGAACGACAGCCAGATATATAACGTTTCTTCTTAAAGATAATTTATTCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAG  >  NC_000913/2109363‑2109623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑