Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,178,586 G→T 5.3% intergenic (‑22/‑235) fbaB ← / → yegT fructose‑bisphosphate aldolase class I/nucleoside transporter, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,178,5860GT 5.3% 260.9 / 2.0 75intergenic (‑22/‑235)fbaB/yegTfructose‑bisphosphate aldolase class I/nucleoside transporter, low affinity
Reads supporting (aligned to +/- strand):  ref base G (47/24);  new base T (2/2);  total (49/26)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAA  >  NC_000913/2178454‑2178717
                                                                                                                                    |                                                                                                                                   
gTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAATGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGat                                                                                                                              >  2:1479678/1‑140 (MQ=255)
  cTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGatat                                                                                                                            <  1:1279920/140‑1 (MQ=255)
   tACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATc                                                                                                                           >  1:637316/1‑140 (MQ=255)
       tAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCt                                                                                                                       <  1:1468909/140‑1 (MQ=255)
          tCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACACCGGTGCTGTTAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTCTGCTCCCGTAAATTCCGATTGGATATCGGCTATg                                                                                                                    <  2:1565353/140‑1 (MQ=255)
          tCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATg                                                                                                                    <  2:1165501/140‑1 (MQ=255)
          tCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATg                                                                                                                    <  1:1566148/140‑1 (MQ=255)
          tCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGAAAATTCCGATTGGATATCGGCTATg                                                                                                                    <  1:1357992/140‑1 (MQ=255)
           cATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATgg                                                                                                                   >  1:1180340/1‑140 (MQ=255)
           cATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATgg                                                                                                                   >  1:352215/1‑140 (MQ=255)
           cATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATgg                                                                                                                   >  1:1178173/1‑140 (MQ=255)
           cATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATgg                                                                                                                   >  1:151410/1‑140 (MQ=255)
            aTGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAACCGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACGATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGa                                                                                                                  <  1:1609211/140‑1 (MQ=255)
            aTGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGa                                                                                                                  <  1:319931/140‑1 (MQ=255)
            aTGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGa                                                                                                                  <  1:236637/140‑1 (MQ=255)
            aTGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGa                                                                                                                  <  1:236495/140‑1 (MQ=255)
            aTGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGa                                                                                                                  <  1:183812/140‑1 (MQ=255)
             tGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGAt                                                                                                                 >  2:873674/1‑140 (MQ=255)
             tGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGCTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAATCAACTGCGCAATATCTGTCATTACTATCCTCCCGTAAATTCCGATTGGATAACGGCTATGGAt                                                                                                                 >  2:676684/1‑140 (MQ=255)
               tCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTg                                                                                                               >  1:627633/1‑140 (MQ=255)
               tCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTg                                                                                                               >  2:967709/1‑140 (MQ=255)
                                 gTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGc                                                                                             >  2:1256998/1‑140 (MQ=255)
                                     gAAGGAATTGTCATACACCGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGc                                                                                         <  1:1361039/140‑1 (MQ=255)
                                     gAAGGAATTGTCATACAACGGTGCTGTTAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGc                                                                                         <  2:593562/140‑1 (MQ=255)
                                     gAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGc                                                                                         <  2:1572084/140‑1 (MQ=255)
                                     gAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGc                                                                                         <  2:405577/140‑1 (MQ=255)
                                     gAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGc                                                                                         <  1:426680/140‑1 (MQ=255)
                                          aaTTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAATTTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCa                                                                                    <  1:437067/140‑1 (MQ=255)
                                          aaTTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCa                                                                                    <  1:1678322/140‑1 (MQ=255)
                                           aTTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCAt                                                                                   <  2:1036620/140‑1 (MQ=255)
                                           aTTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGAAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCAt                                                                                   >  2:1328402/1‑140 (MQ=255)
                                                cATACAACGGTGCTGTAAAAGGTTGTCTGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCTATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCggg                                                                                          >  1:35427/1‑128 (MQ=255)
                                                cATACAACGGTGCTGTAAAAGGTTGTCTGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCTATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCggg                                                                                          <  2:35427/128‑1 (MQ=255)
                                                cATACAACGGTGCTGTAAAAGGTTGTCTGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCTATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCggg                                                                                          >  1:32336/1‑128 (MQ=255)
                                                cATACAACGGTGCTGTAAAAGGTTGTCTGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCTATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCggg                                                                                          <  2:32336/128‑1 (MQ=255)
                                                    cAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCCAGCAACTGCGCAATATCTGTCATTACTATGCTCCTGTAAATTCCGATTGGATATCGGCTATGGATTGTCATGGCCCGCCTTTTGCGGGCAATCATACTCCTGGt                                                                          >  2:1269725/1‑140 (MQ=255)
                                                    cAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGt                                                                          <  1:1310430/140‑1 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  2:798161/1‑140 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  1:1156511/1‑140 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  1:1007882/1‑140 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  1:913289/1‑140 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  1:1168525/1‑140 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  1:1156633/1‑140 (MQ=255)
                                                         gTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTa                                                                     >  1:1538863/1‑140 (MQ=255)
                                                                 aaaGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAg                                                             <  1:1321643/140‑1 (MQ=255)
                                                                      ttGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCAAAATCCTGGTCGTTACGGGCGAGttttt                                                        >  2:518734/1‑140 (MQ=255)
                                                                       tGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTc                                                       >  1:761618/1‑140 (MQ=255)
                                                                         tCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCa                                                     >  1:1235933/1‑140 (MQ=255)
                                                                         tCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCa                                                     >  2:953857/1‑140 (MQ=255)
                                                                         tCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCa                                                     >  2:954906/1‑140 (MQ=255)
                                                                                         gCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGttttt                                     >  1:1298577/1‑140 (MQ=255)
                                                                                         gCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGttttt                                     >  1:1234738/1‑140 (MQ=255)
                                                                                          cAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGAGTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCGTTTGGATGtttttt                                    >  2:531546/1‑140 (MQ=255)
                                                                                          cAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGAAATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGtttttt                                    >  2:937294/1‑140 (MQ=255)
                                                                                          cAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGAGTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGtttttt                                    >  2:937373/1‑140 (MQ=255)
                                                                                            aCTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCa                                  <  1:616851/140‑1 (MQ=255)
                                                                                               gcgcAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCACTCATAGACCTGGTCGTTTCGGGCGAGGTTTTGCTTCGTTGGGATGTTTGTTGAggg                               >  2:1512627/1‑138 (MQ=255)
                                                                                               gcgcAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCg                               >  2:861935/1‑140 (MQ=255)
                                                                                               gcgcAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCg                               >  1:820862/1‑140 (MQ=255)
                                                                                                 gcAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGtt                             <  2:1017294/140‑1 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAATTCCGTTTGGATATCGGCTATGGATTGTCCTGGCCCGCCTATTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGGTTTTCCATCATTTGGATGTATTTTCAGCGTTTTCGGTTgg                    >  2:709992/1‑140 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTTGATGTTTTTTCTGCGTTTTCTGTTgg                    >  2:869082/1‑140 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTgg                    >  2:519064/1‑140 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTgg                    >  2:294047/1‑140 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTgg                    >  1:1667022/1‑140 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTgg                    >  2:1443976/1‑140 (MQ=255)
                                                                                                          gTCATTACTATGCTCCCGTAAAATCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTgg                    >  2:710009/1‑140 (MQ=255)
                                                                                                               tACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTGGGATGTTGTTTCAGCGTTTTCTGTTGGCTCGa               >  2:596701/1‑140 (MQ=255)
                                                                                                               tACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTATTTCCATCTGTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGa               >  2:1235943/1‑140 (MQ=255)
                                                                                                               tACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTAGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGAATTTTCAGCGTTTTCTGTTGGCTCGa               >  2:596062/1‑140 (MQ=255)
                                                                                                               tACTATGCACCCGTAAAATCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTATCCATCTATTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGa               >  2:596057/1‑140 (MQ=255)
                                                                                                                     gCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATtcatc         >  2:1083021/1‑140 (MQ=255)
                                                                                                                         ccGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGaa     >  2:1502755/1‑140 (MQ=255)
                                                                                                                          cGTAAATTCCGAATGGATATCGGCCATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTGTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGaaa    <  1:1251558/140‑1 (MQ=255)
                                                                                                                            tAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGaaaaa  >  2:1582118/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAA  >  NC_000913/2178454‑2178717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: