Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,264,865 C→A 6.0% P335T (CCC→ACC)  setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,264,8650CA 6.0% 237.0 / 2.1 66P335T (CCC→ACC) setBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base C (24/38);  new base A (2/2);  total (26/40)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGAT  >  NC_000913/2264728‑2265001
                                                                                                                                         |                                                                                                                                        
ccGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGccc                                                                                                                                        <  2:623050/140‑1 (MQ=255)
   tgggtggCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGt                                                                                                                                     <  1:740864/140‑1 (MQ=255)
     ggtggCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCa                                                                                                                                   >  1:287472/1‑140 (MQ=255)
     ggtggCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCa                                                                                                                                   >  1:225966/1‑140 (MQ=255)
          cGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCgg                                                                                                                              >  1:1635629/1‑140 (MQ=255)
          cGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCgg                                                                                                                              >  1:1635648/1‑140 (MQ=255)
           gTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCggg                                                                                                                             <  1:1260138/140‑1 (MQ=255)
              tGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTc                                                                                                                          >  1:463470/1‑140 (MQ=255)
                     aCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGccacc                                                                                                                   <  1:1325752/140‑1 (MQ=255)
                     aCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGccacc                                                                                                                   <  2:820366/140‑1 (MQ=255)
                     aCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGccacc                                                                                                                   <  1:1126453/140‑1 (MQ=255)
                     aCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGccacc                                                                                                                   <  2:821930/140‑1 (MQ=255)
                     aCGCAGGAATGCTGATGGCGCAGTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGccacc                                                                                                                   <  2:822058/140‑1 (MQ=255)
                      cGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGccacca                                                                                                                  <  2:802502/140‑1 (MQ=255)
                        cAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACg                                                                                                                <  1:1489917/140‑1 (MQ=255)
                         aGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGc                                                                                                               <  1:1599986/140‑1 (MQ=255)
                         aGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGc                                                                                                               <  1:499147/140‑1 (MQ=255)
                         aGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGc                                                                                                               <  2:1017109/140‑1 (MQ=255)
                           gAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGctc                                                                                                             <  2:1301091/140‑1 (MQ=255)
                           gAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGctc                                                                                                             <  1:530522/140‑1 (MQ=255)
                           gAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGctc                                                                                                             <  1:530519/140‑1 (MQ=255)
                           gAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGctc                                                                                                             <  1:1038729/140‑1 (MQ=255)
                           gAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGctc                                                                                                             >  1:273269/1‑140 (MQ=255)
                           gAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGctc                                                                                                             <  2:1460725/140‑1 (MQ=255)
                                   aTGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCaa                                                                                                     >  1:1581679/1‑140 (MQ=255)
                                   aTGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCaa                                                                                                     <  2:393962/140‑1 (MQ=255)
                                     ggCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAaca                                                                                                   >  1:1324675/1‑140 (MQ=255)
                                        gcATTCAGCTGTCATTCAGTTGGGCTTGCAGCTGCTTAATGCTATATTTATTGGCATTCTGGGCGGCAACGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTACAGCCACCACGCTCTAAACCAACACta                                                                                                <  2:1426306/140‑2 (MQ=255)
                                          aTTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTcg                                                                                              >  1:476812/1‑140 (MQ=255)
                                              aCCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTcgcgcg                                                                                          <  1:579548/140‑1 (MQ=255)
                                                        cTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGat                                                                                >  1:645898/1‑140 (MQ=255)
                                                          gTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGatca                                                                              >  2:864356/1‑140 (MQ=255)
                                                                   gCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGACCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGAt                                                                     >  1:1398707/1‑140 (MQ=255)
                                                                   gCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGACCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGAt                                                                     >  1:1398700/1‑140 (MQ=255)
                                                                                 cTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCAtc                                                       >  1:339205/1‑140 (MQ=255)
                                                                                    tttttATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCTGGCAtcgtc                                                    >  1:668032/1‑140 (MQ=255)
                                                                                       ttATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGcc                                                 <  2:596413/140‑1 (MQ=255)
                                                                                       ttATTGCCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGcc                                                 <  1:745420/140‑1 (MQ=255)
                                                                                         aTTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCga                                               >  2:361907/1‑140 (MQ=255)
                                                                                                  cTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTATATACCAACACTTCGCGCGTGGGCTGGATCATAGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGaa                                      <  1:94645/140‑1 (MQ=255)
                                                                                                          cATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGTGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                              <  2:65705/140‑1 (MQ=255)
                                                                                                          cATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                              <  1:937791/140‑1 (MQ=255)
                                                                                                          cATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                              <  1:970601/140‑1 (MQ=255)
                                                                                                          cATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                              <  2:1086786/140‑1 (MQ=255)
                                                                                                          cATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACg                              <  1:1346037/140‑1 (MQ=255)
                                                                                                            tCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCt                            <  1:1143242/140‑1 (MQ=255)
                                                                                                            tCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCt                            <  2:466239/140‑1 (MQ=255)
                                                                                                            tCGGGATGCTCTATCTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCt                            <  1:1075405/140‑1 (MQ=255)
                                                                                                                    ctctATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGATTTATCACGCTGTGTTCTg                    >  2:18413/1‑140 (MQ=255)
                                                                                                                    ctctATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATAACGCTGTGTTCTg                    >  1:1445267/1‑140 (MQ=255)
                                                                                                                      ctATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGt                  <  2:1515914/140‑1 (MQ=255)
                                                                                                                       tATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCTCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGtt                 >  1:709029/1‑140 (MQ=255)
                                                                                                                       tATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGtt                 >  1:1044818/1‑140 (MQ=255)
                                                                                                                       tATTTACAGGACCTGCTGCCCGGTCAGGCGGGATCAGCCACAACGCTCTATCCCAACACTTCGCGCGAGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTACTGGtt                 <  2:1605254/140‑1 (MQ=255)
                                                                                                                           ttCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCTCGCTGTGTTCTGGTTTGCg             <  2:1551404/140‑1 (MQ=255)
                                                                                                                           ttCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCg             <  2:524601/140‑1 (MQ=255)
                                                                                                                           ttCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCAGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCg             <  2:1551090/140‑1 (MQ=255)
                                                                                                                           ttCAGGATCTGATGACCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATAACGCTGTGTTCTGGTTTGCg             <  2:1398707/140‑1 (MQ=255)
                                                                                                                           ttCAGGATCTGATGACCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATAACGCTGTGTTCTGGTTTGCg             <  2:1398700/140‑1 (MQ=255)
                                                                                                                            tCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGtt                       <  2:365223/129‑1 (MQ=255)
                                                                                                                            tCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGtt                       >  1:365223/1‑129 (MQ=255)
                                                                                                                              aGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATg          >  1:1447375/1‑140 (MQ=255)
                                                                                                                              aGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATg          >  1:1468638/1‑140 (MQ=255)
                                                                                                                              aGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGGTCTGGGTTGCGATg          >  1:618510/1‑140 (MQ=255)
                                                                                                                              aGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCGGTGTTCTGGTTTGCGATg          >  1:1468631/1‑140 (MQ=255)
                                                                                                                                gATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGt        >  2:707192/1‑140 (MQ=255)
                                                                                                                                      aTGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTgatgat  <  2:1511333/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGATGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGAT  >  NC_000913/2264728‑2265001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: