Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,364,318 G→T 6.6% intergenic (‑43/‑236) yfaZ ← / → nudI outer membrane protein, putative porin/nucleoside triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,364,3180GT 6.6% 216.7 / 2.3 68intergenic (‑43/‑236)yfaZ/nudIouter membrane protein, putative porin/nucleoside triphosphatase
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base T (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAAATAATAAT  >  NC_000913/2364179‑2364455
                                                                                                                                           |                                                                                                                                         
tgtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                                                                                                                                           <  2:998533/140‑1 (MQ=255)
tgtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                                                                                                                                           <  2:1280427/140‑1 (MQ=255)
tgtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                                                                                                                                           <  2:1280398/140‑1 (MQ=255)
tgtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                                                                                                                                           <  1:1172976/140‑1 (MQ=255)
 gtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGc                                                                                                                                          >  2:1353227/1‑140 (MQ=255)
 gtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGc                                                                                                                                          >  1:958503/1‑140 (MQ=255)
 gtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGc                                                                                                                                          >  1:715274/1‑140 (MQ=255)
 gtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGc                                                                                                                                          >  1:1641852/1‑140 (MQ=255)
 gtATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATACCCAAAGCTGc                                                                                                                                          >  2:754014/1‑140 (MQ=255)
  tATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCg                                                                                                                                         >  1:1441903/1‑140 (MQ=255)
    ttCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCggg                                                                                                                                       >  1:1315991/1‑140 (MQ=255)
     tCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGCCCGCTGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGa                                                                                                                                      <  2:454591/140‑1 (MQ=255)
         tACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGt                                                                                                                                  >  2:189654/1‑140 (MQ=255)
          aCCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa                                                                                                                                 <  1:1261771/140‑1 (MQ=255)
          aCCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa                                                                                                                                 <  2:1559444/140‑1 (MQ=255)
          aCCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa                                                                                                                                 <  1:702822/140‑1 (MQ=255)
          aCCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa                                                                                                                                 <  1:912936/140‑1 (MQ=255)
          aCCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa                                                                                                                                 <  1:912864/140‑1 (MQ=255)
          aCCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATCTTGTTCATTATCCCCTTTCCCACAAGCAATTACTCAAAATGCCCAAAGCTGCGGGATTGTa                                                                                                                                 <  1:1264067/140‑1 (MQ=255)
               ccTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGcc                                                                                                                            >  1:68356/1‑140 (MQ=255)
               ccTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGcc                                                                                                                            <  2:808347/140‑1 (MQ=255)
               ccTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCAACAAGCAATTAATCAAAATTCCCAAAGCTg                                                                                                                                           >  1:587581/1‑125 (MQ=255)
               ccTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCAACAAGCAATTAATCAAAATTCCCAAAGCTg                                                                                                                                           <  2:587581/125‑1 (MQ=255)
                 tGACCGCTGATGCGCATTGAATTGACCGATGCAGAAACAAACAGCATTCATGCAAGACATGATAGCGCAAATGTTTGCTTTATCCCCTTTCCCACATGCAATTTATCAAAATTCCAAAAGCTGCGGGATTGTACTGCCTg                                                                                                                          >  2:1299469/1‑140 (MQ=255)
                       cTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACag                                                                                                                    <  1:126512/140‑1 (MQ=255)
                                    cattgACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAg                                                                                                       >  2:497132/1‑140 (MQ=255)
                                     attgACCGTTGCATAAAACAACAGCATTCCGGCCAGACCTTCTTTCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGc                                                                                                      <  2:1186111/140‑1 (MQ=255)
                                         aCCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGaaa                                                                                                  >  2:410797/1‑140 (MQ=255)
                                         aCCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGaaa                                                                                                  >  1:1248967/1‑140 (MQ=255)
                                         aCCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGaaa                                                                                                  >  2:579284/1‑140 (MQ=255)
                                         aCCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGaaa                                                                                                  >  1:1449548/1‑140 (MQ=255)
                                         aCCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGaaa                                                                                                  >  1:1657192/1‑140 (MQ=255)
                                              tGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAg                                                                                             >  1:5000/1‑140 (MQ=255)
                                              tGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAg                                                                                             >  2:319937/1‑140 (MQ=255)
                                              tGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAg                                                                                             >  1:5094/1‑140 (MQ=255)
                                              tGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAg                                                                                             >  2:1590635/1‑140 (MQ=255)
                                              tGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAg                                                                                             >  2:955864/1‑140 (MQ=255)
                                                   aaaCCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCt                                                                                        >  2:1455040/1‑140 (MQ=255)
                                                   aaaCCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAAATAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGTATTCAATGAGCGAAAAAAAGGATCt                                                                                        >  2:336435/1‑140 (MQ=255)
                                                    aaCCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTa                                                                                       <  1:294803/140‑1 (MQ=255)
                                                    aaCCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTa                                                                                       <  1:294860/140‑1 (MQ=255)
                                                             cATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTAAt                                                                              >  1:1675578/1‑140 (MQ=255)
                                                               ttCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTg                                                                            <  2:1190845/140‑1 (MQ=255)
                                                               ttCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTg                                                                            <  1:1233536/140‑1 (MQ=255)
                                                                tCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGt                                                                           >  2:653343/1‑140 (MQ=255)
                                                                tCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGt                                                                           >  2:650761/1‑140 (MQ=255)
                                                                tCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGt                                                                           >  2:650729/1‑140 (MQ=255)
                                                                       aGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTaaa                                                                    >  2:1456881/1‑140 (MQ=255)
                                                                       aGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTaaa                                                                    >  2:32853/1‑140 (MQ=255)
                                                                       aGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCAGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTaaa                                                                    <  1:189654/140‑1 (MQ=255)
                                                                       aGACCTGCTAGCGCAATTTTTTTCATTATCCCCTGTCCCACCAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAAGGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTaaa                                                                    <  2:1496558/140‑1 (MQ=255)
                                                                              cTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATActtct                                                             >  1:453779/1‑140 (MQ=255)
                                                                              cTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATActtct                                                             >  1:453780/1‑140 (MQ=255)
                                                                                 gcgcAATTTTTTTCATTATCCCCTGTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTaaa                                                         <  1:1112945/140‑1 (MQ=255)
                                                                                 gcgcAATTTTTTTAATAATCCCCTTTCCCACAAGCATTTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAGGATCTAGTCACTTATTGTAAATAAATACTTCTTaaa                                                         <  1:1112379/140‑1 (MQ=255)
                                                                                  cgcAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTaaa                                                         <  1:32721/140‑1 (MQ=255)
                                                                                   gcAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCATAaat                                                        >  2:160246/1‑140 (MQ=255)
                                                                                   gcAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATACAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTACCTAAATACTTCTTAaat                                                        >  2:160289/1‑140 (MQ=255)
                                                                                                   tCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACTGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGaaaa                                        <  2:68356/140‑1 (MQ=255)
                                                                                                      ccTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAACTAATTAAATGCCGAAAAAAt                                     <  2:696305/140‑1 (MQ=255)
                                                                                                        tttCCCACAAGCAATTAATCAAAATTCCCAAAGCTTCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTa                                                                                       <  1:1022594/88‑1 (MQ=255)
                                                                                                        tttCCCACAAGCAATTAATCAAAATTCCCAAAGCTTCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTa                                                                                       >  2:1022590/1‑88 (MQ=255)
                                                                                                        tttCCCACAAGCAATTAATCAAAATTCCCAAAGCTTCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTa                                                                                       >  2:1022594/1‑88 (MQ=255)
                                                                                                        tttCCCACAAGCAATTAATCAAAATTCCCAAAGCTTCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTa                                                                                       <  1:1022590/88‑1 (MQ=255)
                                                                                                                 aGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAAGGCCGAAAAAATAAACACACttt                          >  1:414896/1‑140 (MQ=255)
                                                                                                                        aaTCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATa                   <  2:507471/140‑1 (MQ=255)
                                                                                                                              aaTTCCCAAATCTGCGGGATTGTACTGCATGGCACAGAGGATTCAATGAGAGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATAcacaca             <  2:919302/140‑1 (MQ=255)
                                                                                                                              aaTTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATAcacaca             <  2:1173446/140‑1 (MQ=255)
                                                                                                                                    cAAAGCTTCGGGATTGTACTGCCTGGAACAGAGGTTTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaata       <  2:1675578/140‑1 (MQ=255)
                                                                                                                                      aaGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaataat     <  2:1558692/140‑1 (MQ=255)
                                                                                                                                      aaGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaataat     <  2:1411049/140‑1 (MQ=255)
                                                                                                                                       aGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGAGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaataata    <  2:1012171/140‑1 (MQ=255)
                                                                                                                                         cTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaataataat  <  2:1360445/140‑1 (MQ=255)
                                                                                                                                         cTGCGGGATTGTACTGACTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaataataat  <  2:119928/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAAATAATAAT  >  NC_000913/2364179‑2364455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: