Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,637,191 C→A 6.5% D56Y (GAT→TAT)  der ← GTPase; multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,1910CA 6.5% 317.8 / 6.4 92D56Y (GAT→TAT) derGTPase; multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base C (43/43);  new base A (3/3);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

ATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGC  >  NC_000913/2637052‑2637328
                                                                                                                                           |                                                                                                                                         
aTCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATc                                                                                                                                           >  2:674373/1‑140 (MQ=255)
 acGCTTCATCTGCCGGCAGCAGTCCCGCGCTAGCATCCACCATAAACAGTACGACGTCCGCTTCTTCACTCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCa                                                                                                                                          >  2:627984/2‑140 (MQ=255)
    cTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATa                                                                                                                                       <  1:556633/140‑1 (MQ=255)
    cTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATa                                                                                                                                       <  2:385586/140‑1 (MQ=255)
               ggCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTc                                                                                                                            >  2:1179191/1‑140 (MQ=255)
                gCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCa                                                                                                                           <  1:409384/140‑1 (MQ=255)
                  aTCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACg                                                                                                                         >  2:369636/1‑140 (MQ=255)
                           gcgcgcgcATCCACCATACACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAAt                                                                                                                <  2:1678965/140‑1 (MQ=255)
                            cgcgcgcATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAAtt                                                                                                               >  2:1022206/1‑140 (MQ=255)
                            cgcgcgcATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTGTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAAtt                                                                                                               >  1:1018415/1‑140 (MQ=255)
                            cgcgcgcATCCACAATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCAGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATAAATACAGATAAACTCACGGCCTTCAAtt                                                                                                               >  2:1284116/1‑140 (MQ=255)
                            cgcgcgcATCCACAATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCAGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATAAATACAGATAAACTCACGGCCTTCAAtt                                                                                                               >  2:484058/1‑140 (MQ=255)
                            cgcgcgcATCCACAATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCAGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATAAATACAGATAAACTCACGGCCTTCAAtt                                                                                                               >  2:1284027/1‑140 (MQ=255)
                                cgcATCCACCATAACCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCg                                                                                                           <  1:944298/140‑1 (MQ=255)
                                  cATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCa                                                                                                         <  1:1458065/140‑1 (MQ=255)
                                  cATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCa                                                                                                         <  1:1606260/140‑1 (MQ=255)
                                  cATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCa                                                                                                         <  1:1606266/140‑1 (MQ=255)
                                    tCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACg                                                                                                       <  1:34953/140‑1 (MQ=255)
                                     ccaccaTAACCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGa                                                                                                      <  2:1592226/140‑1 (MQ=255)
                                      caccaTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGAc                                                                                                     <  2:1492223/140‑1 (MQ=255)
                                      caccaTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGAc                                                                                                     <  2:307628/140‑1 (MQ=255)
                                      caccaTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGAc                                                                                                     <  1:415812/140‑1 (MQ=255)
                                      caccaTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGAc                                                                                                     <  2:1492270/140‑1 (MQ=255)
                                      caccaTAAACAGGACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGAc                                                                                                     <  1:416312/140‑1 (MQ=255)
                                         caTAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGATCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGTCCTTCAAATTCCGCACGACCGt                                                                                                  >  2:848616/1‑140 (MQ=255)
                                              aCAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTa                                                                                             <  1:1114791/140‑1 (MQ=255)
                                               cAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGGTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGGACTTAc                                                                                            >  2:178027/1‑140 (MQ=255)
                                                 gTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGGTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACgg                                                                                          >  1:1547490/1‑140 (MQ=255)
                                                  tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACTGt                                                                                         >  1:1432093/1‑140 (MQ=255)
                                                  tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGt                                                                                         >  1:1103176/1‑140 (MQ=255)
                                                  tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGt                                                                                         >  2:94805/1‑140 (MQ=255)
                                                  tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGt                                                                                         >  1:633159/1‑140 (MQ=255)
                                                  tACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCACCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGt                                                                                         >  1:1686490/1‑140 (MQ=255)
                                                   acgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCa                                                                                                         <  1:403066/123‑1 (MQ=255)
                                                   acgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCa                                                                                                         >  2:403066/1‑123 (MQ=255)
                                                   acgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTc                                                                                        >  1:359343/1‑140 (MQ=255)
                                                   acgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTc                                                                                        >  1:359272/1‑140 (MQ=255)
                                                    cgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCa                                                                                       <  1:1129817/140‑1 (MQ=255)
                                                    cgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCa                                                                                       <  2:1690550/140‑1 (MQ=255)
                                                    cgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCa                                                                                       <  1:1369615/140‑1 (MQ=255)
                                                    cgacgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCa                                                                                       <  1:1261619/140‑1 (MQ=255)
                                                       cgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGa                                                                                    >  2:1382995/1‑140 (MQ=255)
                                                       cgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGa                                                                                    >  1:952362/1‑140 (MQ=255)
                                                       cgTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGa                                                                                    >  2:1632291/1‑140 (MQ=255)
                                                          ccGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTc                                                                                 >  1:1491284/1‑140 (MQ=255)
                                                          ccGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTc                                                                                 >  2:872753/1‑140 (MQ=255)
                                                          ccGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTc                                                                                 >  2:948412/1‑140 (MQ=255)
                                                            gCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAg                                                                               >  2:1279586/1‑140 (MQ=255)
                                                            gCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAg                                                                               >  2:375567/1‑140 (MQ=255)
                                                            gCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAg                                                                               >  2:376119/1‑140 (MQ=255)
                                                            gCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCAGCCGGTATCAATACAGATAAACTCACGGCCTTCAAGTTCCGCACGACCGTACTTACGGTCACGAGTCAg                                                                               >  2:1335267/1‑140 (MQ=255)
                                                             cttcttCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGa                                                                              <  1:191421/140‑1 (MQ=255)
                                                                   cAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCggg                                                                        >  1:445933/1‑140 (MQ=255)
                                                                    aaTCGCCAGCAGCGACTGTTCCGCCAGGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGa                                                                       <  2:871176/140‑1 (MQ=255)
                                                                          cagcagCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTACAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCg                                                                 >  2:820650/1‑140 (MQ=255)
                                                                                 gACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAAGTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAg                                                          <  2:572251/140‑1 (MQ=255)
                                                                                  aCTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAgc                                                         >  2:588173/1‑140 (MQ=255)
                                                                                   cagtaccGCCATGCGGGTTTCTACACCGTCTTCTGTGCCAACACTCCCTCCGGTATCAATACCGATACACACACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAgcg                                                        <  2:387401/135‑1 (MQ=255)
                                                                                             aTGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGt                                              <  1:197448/140‑1 (MQ=255)
                                                                                              tGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAg                                                                                   <  2:1142008/102‑1 (MQ=255)
                                                                                              tGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTg                                             >  1:610437/1‑140 (MQ=255)
                                                                                              tGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTAACAAGACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACGTACGGTCACGAGTCAGAGCCGGGAATGCCGCAACCAGCGCATCTCGGGTg                                             >  1:731289/1‑140 (MQ=255)
                                                                                              tGCGGGTTTCTACACCGTCTTCTGAGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAg                                                                                   >  1:1142008/1‑102 (MQ=255)
                                                                                              ggCGGGTTTCTCCACCGTCTGCTGTGCCATCTATCCCGCCGGGATCCATACAGCTAACCTCCCGGCCGTCCCGTGCCGCACGCCCGTACTGACGGTCACGAGTCCGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTg                                             <  2:374212/139‑1 (MQ=255)
                                                                                                     ttCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTa                                      <  1:235422/140‑1 (MQ=255)
                                                                                                     ttCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTa                                      <  1:1536165/140‑1 (MQ=255)
                                                                                                     ttCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTa                                      <  2:355220/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  1:1022206/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  1:1168771/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  2:1031392/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  1:1580284/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATAAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  1:484058/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATAAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  1:1284027/140‑1 (MQ=255)
                                                                                                       cTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATAAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGa                                    <  1:1284116/140‑1 (MQ=255)
                                                                                                         acacCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACg                                  <  1:297796/140‑1 (MQ=255)
                                                                                                             cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGGCGGTTa                              >  2:1420501/1‑140 (MQ=255)
                                                                                                             cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTa                              >  2:1036176/1‑140 (MQ=255)
                                                                                                              gTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTaa                             <  2:76696/140‑1 (MQ=255)
                                                                                                              gTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTaa                             <  2:76697/140‑1 (MQ=255)
                                                                                                               tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTaaa                            >  1:1315762/1‑140 (MQ=255)
                                                                                                                         cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACTGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAt                  <  2:1432093/140‑1 (MQ=255)
                                                                                                                         cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAt                  <  1:409034/140‑1 (MQ=255)
                                                                                                                         cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAt                  <  1:24525/140‑1 (MQ=255)
                                                                                                                           tCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAttt                >  1:939025/1‑140 (MQ=255)
                                                                                                                           tCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAttt                >  1:436964/1‑140 (MQ=255)
                                                                                                                           tCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGAttt                >  1:940246/1‑140 (MQ=255)
                                                                                                                           tCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGGTAAATAACGTGGAttt                >  1:385319/1‑140 (MQ=255)
                                                                                                                             aaTCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTc              <  1:636238/140‑1 (MQ=255)
                                                                                                                             aaTCCCGCCGGTATCAATACAGATAAACTCACGGACTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCCGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTc              <  1:804187/140‑1 (MQ=255)
                                                                                                                                cccGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTa           >  2:1429148/1‑140 (MQ=255)
                                                                                                                                  cgccgGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACg         >  1:691027/1‑140 (MQ=255)
                                                                                                                                         aTCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGgc  >  1:61185/1‑140 (MQ=255)
                                                                                                                                         aTCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGgc  >  1:61492/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
ATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCGCTTCTTCAATCGCCAGCAGCGACTGTTCCGCCATGCGGGTTTCTACACCGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGC  >  NC_000913/2637052‑2637328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: