Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,650,199 C→A 11.8% E697* (GAA→TAA)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,650,1990CA11.8% 273.6 / 15.4 90E697* (GAA→TAA) yfhMbacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (52/27);  new base A (6/5);  total (58/32)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATA  >  NC_000913/2650061‑2650334
                                                                                                                                          |                                                                                                                                       
aGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCg                                                                                                                                        >  2:120416/1‑140 (MQ=255)
  cTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGAt                                                                                                                                      <  2:189936/140‑1 (MQ=255)
     taGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAg                                                                                                                                   <  1:379158/140‑1 (MQ=255)
        ccacccCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGATTCGTCCGCTTTCAGATCCAGAGTTTGTACATTTTCGATCAGATc                                                                                                                                >  2:926795/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGTCGATAAGCGCCCCACACAACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATGTGCGGGCAGATCtgtt                                                                                                                            >  2:466912/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCtttt                                                                                                                            >  2:1195139/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCtttt                                                                                                                            >  1:822539/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCtttt                                                                                                                            >  1:1007013/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCtttt                                                                                                                            >  1:324180/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCtttt                                                                                                                            >  1:271022/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGGTTGTTCATTTTCGATCAGATCtttt                                                                                                                            >  2:394376/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTACGCTTTCAGATCCAGAGTTTGTTCATTTTAGATCAGATCtttt                                                                                                                            >  2:21221/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTACGCTTTCAGATCCAGAGTTTGTTCATTTTAGATCAGATCtttt                                                                                                                            >  2:21126/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTACGCTTTCAGATCCAGAGTTTGTTCATTTTAGATCAGATCtttt                                                                                                                            >  2:21289/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTACGCTTTCAGATCCAGAGTTTGTTCATTTTAGATCAGATCtttt                                                                                                                            >  2:22145/1‑140 (MQ=255)
            cccAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTACGCTTTCAGATCCAGAGTTTGTTCATTTTAGATCAGATCtttt                                                                                                                            >  2:22177/1‑140 (MQ=255)
                      aaCACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACt                                                                                                                  >  2:1572751/1‑140 (MQ=255)
                        cacTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACtgt                                                                                                                <  1:787267/140‑1 (MQ=255)
                             ctGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTg                                                                                                           >  2:482473/1‑140 (MQ=255)
                               gACCGCTTCATTCGGCGCTTTGACTTCCCGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGcc                                                                                                         <  1:1444973/140‑1 (MQ=255)
                                aCCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCa                                                                                                        >  1:285864/1‑140 (MQ=255)
                                aCCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGACCAAACTGTGACTGCCa                                                                                                        >  2:238926/1‑140 (MQ=255)
                                aCCGCTTCATTAGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCa                                                                                                        >  1:527780/1‑140 (MQ=255)
                                 ccGCTTCATTCGGCGCTTTGACTTCCAGACGATTAGCTCCCCACTCTACCGGAAAACTGACCTTTCCGGTTTCGTCCGCTTTCAGATCCAGTGTTTGTTCATTTTCGATCAGTTCTTTTTGATCATACTGTGACTGcctg                                                                                                       <  2:824308/140‑3 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  2:41111/1‑140 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  1:228291/1‑140 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  2:1479115/1‑140 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  1:1361457/1‑140 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  2:1232895/1‑140 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  1:120913/1‑140 (MQ=255)
                                     ttCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTAGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCtt                                                                                                   >  2:335077/1‑140 (MQ=255)
                                      tCATTCGGCGCTTTTACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTc                                                                                                  <  2:715076/140‑1 (MQ=255)
                                      tCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAACCTGACCATGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACGGCCAGCCTTc                                                                                                  <  1:1498673/140‑1 (MQ=255)
                                      tCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTc                                                                                                  <  1:1498705/140‑1 (MQ=255)
                                          tCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCAtct                                                                                              >  1:808527/1‑140 (MQ=255)
                                              cgcTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTg                                                                                          >  2:1251716/1‑140 (MQ=255)
                                              cgcTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTg                                                                                          >  2:1414759/1‑140 (MQ=255)
                                                 tttGACTTCCAGACGATAAGCGCCCCACTCTACCTGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGTTCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGAcc                                                                                       <  1:881044/140‑1 (MQ=255)
                                                 tttGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGAcc                                                                                       <  2:629832/140‑1 (MQ=255)
                                                 tttGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCAGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGAcc                                                                                       <  1:1515585/140‑1 (MQ=255)
                                                   tGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAg                                                                                     >  1:1255223/1‑140 (MQ=255)
                                                        tCCAGACGATAATCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTATCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCGTCATCTTCTGACCAGTTCCa                                                                                >  2:342601/1‑140 (MQ=255)
                                                        tCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCa                                                                                >  2:312074/1‑140 (MQ=255)
                                                                       ccccACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCgcggcg                                                                 >  2:496213/1‑140 (MQ=255)
                                                                       ccccACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCgcggcg                                                                 >  2:496098/1‑140 (MQ=255)
                                                                       ccccACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCgcggcg                                                                 >  1:909452/1‑140 (MQ=255)
                                                                              ctACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGa                                                          <  1:223376/140‑1 (MQ=255)
                                                                               tACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTACCAGAAGTAATCGCGGCGTTCGCGaa                                                         >  1:1538571/1‑140 (MQ=255)
                                                                                 ccGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTACATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATc                                                       >  2:695159/1‑140 (MQ=255)
                                                                                  cGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGTTCTTTTTGATCAAACTGTGACTGCCAGCCTGCATCTTCTGACCAGTACCTGAAGTAATCGCGGCGTTCGCGAAACa                                                      >  2:1639727/1‑140 (MQ=255)
                                                                                  cGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCa                                                      >  2:1304567/1‑140 (MQ=255)
                                                                                  cGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTACAGTTGTAATCGCGGCGTTCGCGAATct                                                      >  2:859166/1‑139 (MQ=255)
                                                                                    gAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAgg                                                    <  2:1187632/140‑1 (MQ=255)
                                                                                    gAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGGTTGTTCAGGTTCGATCAGAGCTTTTAGATCCAACTGTGACTGCCAGCCTGCATCGTCTGACCAGTACCAGGAGGAATCCCGGCTATCGCGAATCAgg                                                    >  2:1303052/1‑140 (MQ=255)
                                                                                    gAAAACTGACATAGCCGGTTTCGTCCGCTGTCTGATCCAGAGTTTGTTCAGTATCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTGATGTCCAGTTCCAGGAGTAATCGCGGCGTTCGCGTATCggg                                                    >  2:989216/1‑140 (MQ=255)
                                                                                      aaaCTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGgcg                                                  <  1:1439531/140‑1 (MQ=255)
                                                                                       aaCTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGgcgc                                                 >  1:1364560/1‑140 (MQ=255)
                                                                                         cTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGAAATCGCGGCGATCGCGAATCAGGCGCAc                                               >  1:457169/1‑140 (MQ=255)
                                                                                           gACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCt                                             >  2:397937/1‑140 (MQ=255)
                                                                                           gACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCt                                             >  2:397933/1‑140 (MQ=255)
                                                                                           gACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCt                                             >  2:1015009/1‑140 (MQ=255)
                                                                                                 gCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGc                                       >  2:670277/1‑140 (MQ=255)
                                                                                                 gCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGc                                       >  2:670588/1‑140 (MQ=255)
                                                                                                 gCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGc                                       >  2:91369/1‑140 (MQ=255)
                                                                                                 gCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGc                                       >  2:91383/1‑140 (MQ=255)
                                                                                                       ttCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGaca                                 <  2:1146523/140‑1 (MQ=255)
                                                                                                       ttCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGaca                                 <  2:1145738/140‑1 (MQ=255)
                                                                                                            ccGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCt                            >  2:1112108/1‑140 (MQ=255)
                                                                                                                          agagTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGAAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTGTTTCACGCCTTg              >  2:1628904/1‑140 (MQ=255)
                                                                                                                          agagTTTGTTCATTTTCGATCAGATCTTGTTGATCAAACTGTCACTGCCAGCCTTCATCTTCTGACCAGTACCAGTAGTAAACGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACCGCTTATTACACGccgag              >  2:1628761/1‑137 (MQ=255)
                                                                                                                           gagTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTgc             <  1:56272/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  2:145365/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:1347581/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:518065/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTAGATCAGTTCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:22177/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTAGATCAGATCTTTTTGCTCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:21289/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTAGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:21126/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTAGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:22145/140‑1 (MQ=255)
                                                                                                                              tttGTTCATTTTAGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:21221/140‑1 (MQ=255)
                                                                                                                              ttggTTCATATTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCa          <  1:518052/137‑1 (MQ=255)
                                                                                                                               ttGTTCATTTTCTATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCAt         <  2:598390/140‑1 (MQ=255)
                                                                                                                               ttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCAt         <  1:145452/140‑1 (MQ=255)
                                                                                                                               ttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCAt         <  2:1433679/140‑1 (MQ=255)
                                                                                                                               ttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCAt         <  2:359953/140‑1 (MQ=255)
                                                                                                                               ttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCAt         <  1:69032/140‑1 (MQ=255)
                                                                                                                               ttGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCAt         <  1:69161/140‑1 (MQ=255)
                                                                                                                                 gTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCg       >  2:1641107/1‑140 (MQ=255)
                                                                                                                                      ttttCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCtata  >  2:1657271/1‑140 (MQ=255)
                                                                                                                                      ttttCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCtata  >  1:1361072/1‑140 (MQ=255)
                                                                                                                                      ttttCGATCAGATCTTTATGATCAAACTGTTACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGTCTTTCGCGAAACAGGCGCACCTGCAAGCCCGACACGGCTTATTTCACGCCTTGCTCATCGCtata  >  2:693367/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
AGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGACGATAAGCGCCCCACTCTACCGGAAAACTGACCTTGCCGGTTTCGTCCGCTTTCAGATCCAGAGTTTGTTCATTTTCGATCAGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATA  >  NC_000913/2650061‑2650334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: