Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,701,262 G→T 7.7% T156T (ACC→ACA pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,701,2620GT 7.7% 273.3 / 6.7 78T156T (ACC→ACApdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (35/37);  new base T (3/3);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTG  >  NC_000913/2701126‑2701401
                                                                                                                                        |                                                                                                                                           
cTTTCTCGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGgtg                                                                                                                                          <  1:391388/140‑1 (MQ=255)
cTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGgtg                                                                                                                                          <  1:391411/140‑1 (MQ=255)
  ttCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTTGCATCAGCATAGCAACCGGtgtg                                                                                                                                        >  1:1527491/1‑140 (MQ=255)
  ttCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGtgtg                                                                                                                                        >  2:1614948/1‑140 (MQ=255)
      cGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                                                                                                                    <  1:900133/140‑1 (MQ=255)
      cGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                                                                                                                    <  2:925688/140‑1 (MQ=255)
      cGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                                                                                                                    <  2:925687/140‑1 (MQ=255)
      cGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                                                                                                                    <  1:111885/140‑1 (MQ=255)
      cGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                                                                                                                    <  1:111935/140‑1 (MQ=255)
      cGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGAtc                                                                                                                                    <  1:1230302/140‑1 (MQ=255)
         tgtgATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCg                                                                                                                                 >  1:633431/1‑140 (MQ=255)
         tgtgATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCg                                                                                                                                 >  1:389348/1‑140 (MQ=255)
          gtgATAGGTCAGACCGTGTCCGGCGTGAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGa                                                                                                                                >  2:1371525/1‑140 (MQ=255)
             aTAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATaa                                                                                                                             >  1:597379/1‑140 (MQ=255)
              tAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATaaa                                                                                                                            <  2:118633/140‑1 (MQ=255)
                ggTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACg                                                                                                                          <  1:247320/140‑1 (MQ=255)
                 gTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACgg                                                                                                                         >  1:1191435/1‑140 (MQ=255)
                  tCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGt                                                                                                                        <  2:158882/140‑1 (MQ=255)
                  tCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGt                                                                                                                        <  2:158828/140‑1 (MQ=255)
                     gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                                                                                     >  2:1397095/1‑140 (MQ=255)
                     gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                                                                                     >  1:176967/1‑140 (MQ=255)
                     gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                                                                                     >  2:79435/1‑140 (MQ=255)
                     gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                                                                                     >  2:81221/1‑140 (MQ=255)
                     gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                                                                                     >  2:834942/1‑140 (MQ=255)
                     gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                                                                                     >  2:98990/1‑140 (MQ=255)
                         gtgtCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGAGCGCCaa                                                                                                                 >  1:338184/1‑140 (MQ=255)
                           gtCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAAcc                                                                                                               >  1:1649122/1‑140 (MQ=255)
                            tCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCGTCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACct                                                                                                              >  1:1271269/1‑140 (MQ=255)
                            tCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACct                                                                                                              >  2:1364388/1‑140 (MQ=255)
                            tCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACct                                                                                                              >  2:1364890/1‑140 (MQ=255)
                            tCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACct                                                                                                              >  1:446108/1‑140 (MQ=255)
                                  gTTAACTTTCAGACCGAGGCTTGCGGCAACGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCg                                                                                                        <  1:277732/140‑1 (MQ=255)
                                  gTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCg                                                                                                        <  1:774099/140‑1 (MQ=255)
                                  gTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCg                                                                                                        <  1:774057/140‑1 (MQ=255)
                                  gTTAACTTTCAGACCGACGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCg                                                                                                        <  1:1056770/140‑1 (MQ=255)
                                           cAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCGTTGa                                                                                               >  1:1335257/1‑140 (MQ=255)
                                             gACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATc                                                                                             <  2:484686/140‑1 (MQ=255)
                                                                    gcgGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATaaa                                                                      >  1:427142/1‑140 (MQ=255)
                                                                    gcgGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATaaa                                                                      >  1:424057/1‑140 (MQ=255)
                                                                    gcgGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATaaa                                                                      >  1:1172487/1‑140 (MQ=255)
                                                                          ttGGCGATACGCGCCATCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAgaga                                                                <  1:969322/140‑1 (MQ=255)
                                                                          ttGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAgaga                                                                <  1:539084/140‑1 (MQ=255)
                                                                           tGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTTGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGaa                                                               >  1:1035526/1‑140 (MQ=255)
                                                                           tGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTTGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGaa                                                               >  1:1035276/1‑140 (MQ=255)
                                                                           tGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTTGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGaa                                                               >  1:1259272/1‑140 (MQ=255)
                                                                           tGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAATCGGTGCGCCAACCTCTGCCGCGGCTTTGTTCTGCTCTTCATCGGCGTCAAGAATCagagta                                                               >  2:832091/1‑138 (MQ=255)
                                                                                  aCGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGaa                                                        >  2:341904/1‑140 (MQ=255)
                                                                                  aCGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGaa                                                        >  2:340963/1‑140 (MQ=255)
                                                                                  aCGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGaa                                                        >  2:340633/1‑140 (MQ=255)
                                                                                     cgcCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATcc                                                     >  2:147244/1‑140 (MQ=255)
                                                                                         aGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCgac                                                 <  1:81221/140‑3 (MQ=255)
                                                                                         aGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCgac                                                 <  1:79435/140‑3 (MQ=255)
                                                                                          gCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAACCAGAGAAACCTGAATCCCGGCa                                                <  2:1557330/140‑1 (MQ=255)
                                                                                          gCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCa                                                <  1:30757/140‑1 (MQ=255)
                                                                                          gCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCa                                                <  2:571700/140‑1 (MQ=255)
                                                                                          gCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCa                                                <  2:667487/140‑1 (MQ=255)
                                                                                          gCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCa                                                <  2:969096/140‑1 (MQ=255)
                                                                                          gCTCTTGCGCCTGCTCGGCGTCAGATTTGGCATCAGCATAGCAACCGGTGTGGATCTAGATAAACGGAGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCa                                                <  2:348640/140‑1 (MQ=255)
                                                                                           ctctTGCGCCTGTTCGGCGTCAGTTTTGGCATAAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCAt                                               <  1:644090/140‑1 (MQ=255)
                                                                                               tGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCACCCGGTGTGGATCTCGAGAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATACACAGAGAAACCTGAATCCCGGCATCTGc                                           <  2:75116/140‑1 (MQ=255)
                                                                                               tGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATCAACGGTGCGCCAACCTCTGCCGCAGCTTTGAGCTGCTCTTCATCGGCGTCTATAAACAGAGAAACCTGAATCCCGGCATCTGc                                           >  2:277482/1‑140 (MQ=255)
                                                                                               tGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGc                                           <  1:1352490/140‑1 (MQ=255)
                                                                                               tGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCAGAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGc                                           <  2:75138/140‑1 (MQ=255)
                                                                                                 cgcCTGTTAGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCa                                         <  2:767987/140‑1 (MQ=255)
                                                                                                     tGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACg                                     <  1:1669/140‑1 (MQ=255)
                                                                                                      gTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCTGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGt                                    >  2:36953/1‑140 (MQ=255)
                                                                                                      gTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCTGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGt                                    >  2:37381/1‑140 (MQ=255)
                                                                                                      gTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCTGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGt                                    >  2:37213/1‑140 (MQ=255)
                                                                                                      gTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGt                                    >  1:1257934/1‑140 (MQ=255)
                                                                                                                 gTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCACTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATc                         >  1:586253/1‑140 (MQ=255)
                                                                                                                      ggCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCa                    <  1:1099920/140‑1 (MQ=255)
                                                                                                                           cAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTg               <  1:377118/140‑1 (MQ=255)
                                                                                                                            aGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGt              >  1:623/1‑140 (MQ=255)
                                                                                                                                tAGCAACCTGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACg          <  1:36953/140‑1 (MQ=255)
                                                                                                                                tAGCAACCTGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACg          <  1:37213/140‑1 (MQ=255)
                                                                                                                                tAGCAACCTGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACg          <  1:37381/140‑1 (MQ=255)
                                                                                                                                tAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACg          <  1:1477784/140‑1 (MQ=255)
                                                                                                                                tAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACg          <  1:1250841/140‑1 (MQ=255)
                                                                                                                                        ggTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTg  >  2:831740/1‑140 (MQ=255)
                                                                                                                                        ggTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCGTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCCGACGTTTGCTGGCATCGCGCATGTTGTCACGCTGCCCTg  >  2:1131617/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
CTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTG  >  NC_000913/2701126‑2701401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: