Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,753,846 C→A 5.8% P81Q (CCA→CAA)  bamE → lipoprotein component of BamABCDE OM biogenesis complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,753,8460CA 5.8% 298.9 / 2.8 85P81Q (CCA→CAA) bamElipoprotein component of BamABCDE OM biogenesis complex
Reads supporting (aligned to +/- strand):  ref base C (36/44);  new base A (2/2);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGC  >  NC_000913/2753707‑2753982
                                                                                                                                           |                                                                                                                                        
gggAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAAcc                                                                                                                                          <  1:1019120/140‑1 (MQ=255)
 ggAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCa                                                                                                                                         <  1:808817/140‑1 (MQ=255)
       aTCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACAAGGTCAt                                                                                                                                   >  2:513412/1‑140 (MQ=255)
       aTCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACAAGGTCAt                                                                                                                                   >  2:513697/1‑140 (MQ=255)
        tCTTACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATg                                                                                                                                  >  2:1452664/1‑140 (MQ=255)
        tCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCagg                                                                                                                                  >  2:743120/1‑138 (MQ=255)
          tGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGaa                                                                                                                                >  1:512647/1‑140 (MQ=255)
           gACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAg                                                                                                                               >  1:1186358/1‑140 (MQ=255)
                cTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTa                                                                                                                          >  1:816881/1‑140 (MQ=255)
                cTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTa                                                                                                                          >  1:1530855/1‑140 (MQ=255)
                cTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTa                                                                                                                          >  2:1333088/1‑140 (MQ=255)
                cTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTa                                                                                                                          >  1:817151/1‑140 (MQ=255)
                  aaCGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAAc                                                                                                                        >  2:389651/1‑140 (MQ=255)
                        gTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCa                                                                                                                  <  1:618460/140‑1 (MQ=255)
                              acaaTACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgct                                                                                                            <  2:1102700/138‑1 (MQ=255)
                              aaaaTACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgct                                                                                                            <  1:65481/140‑1 (MQ=255)
                                     gtgtTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgctg                                                                                                     <  1:606855/140‑1 (MQ=255)
                                      tgtTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAAcgctgacgctga                                                                                                    >  2:222086/1‑140 (MQ=255)
                                      tgtTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAAcgctgacgctga                                                                                                    <  1:1291476/140‑1 (MQ=255)
                                           gCATGACGCTACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATACATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCttt                                                                                               <  2:1550811/140‑1 (MQ=255)
                                           gCATGACGCAACAACAAGTTGCGTACGCTTTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCttt                                                                                               <  2:1550894/140‑1 (MQ=255)
                                           gCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCttt                                                                                               <  2:1168658/140‑1 (MQ=255)
                                           gCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCttt                                                                                               <  1:389651/140‑1 (MQ=255)
                                           gCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCttt                                                                                               <  2:1550763/140‑1 (MQ=255)
                                           gCAAGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATGCATTTGGTACGAATACCTGGTTATATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACTCTGACGCTGACCttt                                                                                               <  2:1307684/140‑1 (MQ=255)
                                                aCGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAg                                                                                          <  2:1159398/140‑1 (MQ=255)
                                                aCGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAg                                                                                          <  2:1575003/140‑1 (MQ=255)
                                                aCGCAACAACAAGTTGCGTACGCATTGGGTACACCACTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAg                                                                                          <  1:920057/140‑1 (MQ=255)
                                                  gCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTGGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTa                                                                                        <  2:552800/140‑1 (MQ=255)
                                                     acaacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTTGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCg                                                                                     <  2:736314/140‑1 (MQ=255)
                                                     acaacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCg                                                                                     <  1:88098/140‑1 (MQ=255)
                                                     acaacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCg                                                                                     <  2:1379941/140‑1 (MQ=255)
                                                     acaacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCg                                                                                     <  2:734388/140‑1 (MQ=255)
                                                     acaacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCg                                                                                     <  2:1063433/140‑1 (MQ=255)
                                                          aaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGgtgtg                                                                                <  1:86096/140‑1 (MQ=255)
                                                          aaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGgtgtg                                                                                <  1:1446895/140‑1 (MQ=255)
                                                                ctaaagCAATGGGAACACCGATTAAGTAAGATCCAAAACGTAAGAATACCAGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGAcc                                                                          >  2:1257290/6‑140 (MQ=255)
                                                                cGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGAcc                                                                          >  2:1343207/1‑140 (MQ=255)
                                                                    cGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAata                                                                      <  2:1448301/140‑1 (MQ=255)
                                                                      cATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATAtt                                                                    <  1:1204566/140‑1 (MQ=255)
                                                                      cATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATAtt                                                                    <  1:427347/140‑1 (MQ=255)
                                                                      cATTGGGTACACCGCTGATGTCAGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATAtt                                                                    <  1:139053/140‑1 (MQ=255)
                                                                           ggTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATaa                                                               <  1:215805/140‑1 (MQ=255)
                                                                           ggTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATaa                                                               >  2:815114/1‑140 (MQ=255)
                                                                             tACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTTACCAATATTGATAACa                                                             <  2:773332/140‑1 (MQ=255)
                                                                                acCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAAc                                                          <  2:1215305/140‑1 (MQ=255)
                                                                                  cGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCt                                                        >  1:88025/1‑140 (MQ=255)
                                                                                    cTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCCTGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCtcc                                                      >  2:1608563/1‑138 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACTAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTgcg                                                     >  2:366053/1‑140 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGTTAACAAACCTgcg                                                     >  2:1338742/1‑140 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTgcg                                                     >  2:1537221/1‑140 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTgcg                                                     >  1:732221/1‑140 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTgcg                                                     >  2:1338945/1‑140 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTgcg                                                     <  1:222086/140‑1 (MQ=255)
                                                                                     tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTgcg                                                     >  1:913657/1‑140 (MQ=255)
                                                                                       aTGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCt                                                   <  2:816881/140‑1 (MQ=255)
                                                                                       aTGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCt                                                   <  2:817151/140‑1 (MQ=255)
                                                                                       aTGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCt                                                   <  1:1635950/140‑1 (MQ=255)
                                                                                            cGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTg                                              >  1:1422712/1‑140 (MQ=255)
                                                                                              agccATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTTACTGGAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGGGGt                                            >  2:178920/3‑140 (MQ=255)
                                                                                              aTCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGt                                            >  1:541923/1‑140 (MQ=255)
                                                                                              aTCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGt                                            >  2:1277787/1‑140 (MQ=255)
                                                                                              aTCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACAAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGt                                            <  1:513697/140‑1 (MQ=255)
                                                                                              aTCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACAAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGt                                            <  1:513412/140‑1 (MQ=255)
                                                                                               tCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTa                                           >  1:433592/1‑140 (MQ=255)
                                                                                               tCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTa                                           >  1:1360369/1‑140 (MQ=255)
                                                                                                 cATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAAc                                         <  1:1292662/140‑1 (MQ=255)
                                                                                                 cATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAAc                                         <  1:1292968/140‑1 (MQ=255)
                                                                                                 cATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGAACAATATTGATAACAAACCTGCGCTGAGTGGTAAc                                         <  2:216947/140‑1 (MQ=255)
                                                                                                  aTTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTTGGGGTGTGTTGACAATTATTTATAACAAACCTGCGCTGTGTGGTAACt                                        <  1:88562/140‑1 (MQ=255)
                                                                                                         aCGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACtaataata                                 >  1:772531/1‑140 (MQ=255)
                                                                                                            aaTACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAg                              >  2:469381/1‑140 (MQ=255)
                                                                                                             aTACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGt                             >  2:88562/1‑140 (MQ=255)
                                                                                                               aCCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTg                           <  1:516065/140‑1 (MQ=255)
                                                                                                                       cTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCaaa                   <  1:252437/140‑1 (MQ=255)
                                                                                                                       cTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCaaa                   <  2:810376/140‑1 (MQ=255)
                                                                                                                       cTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCaaa                   <  1:1432350/140‑1 (MQ=255)
                                                                                                                        tATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAg                  >  1:45969/1‑140 (MQ=255)
                                                                                                                          tGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGAc                >  2:931744/1‑140 (MQ=255)
                                                                                                                          tGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGAc                >  1:1368325/1‑140 (MQ=255)
                                                                                                                          tGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGAc                >  2:1587128/1‑140 (MQ=255)
                                                                                                                          tGTCTTCCGCCAGCAACCAGGTAATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGAc                >  2:432561/1‑140 (MQ=255)
                                                                                                                          tGTCTTCCGCCAGAAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGATGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGAc                >  1:1188563/1‑140 (MQ=255)
                                                                                                                               tCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTaa           >  1:1566913/1‑140 (MQ=255)
                                                                                                                                      gCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGt    <  2:1422712/140‑1 (MQ=255)
                                                                                                                                        aaCCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGc  <  1:1442405/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGC  >  NC_000913/2753707‑2753982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: