BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,821,107	G→A	5.7%	S302F (TCC→TTC)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,107	0	G	A	5.7%	369.5 / 8.3	105	S302F (TCC→TTC)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (48/51); new base A (3/3); total (51/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 107 total aligned reads displayed.

TCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGTCGATGT  >  NC_000913/2820969‑2821245

tCTTCACCCTCAGAGCCCCTAACGTCGATCCGCGGACCAACCAGTTTGCAGAAGAAGGTTTCTTGCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATCACCACGGTGTTCATTGGa                                                                                                                                           <  2:1177667/140‑1 (MQ=255)

tCTTCACCCGCTGAGCCCATAACGTCGATCAGCGGACATACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCTCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  1:1639735/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  1:453344/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  2:933942/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  2:925406/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  2:1596896/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  1:1183825/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  1:1639780/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  1:816445/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGa                                                                                                                                           <  1:1344967/140‑1 (MQ=255)

tCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAAACTAGGTTTTGTTTGGa                                                                                                                                           <  1:237627/140‑1 (MQ=255)

   tCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACgg                                                                                                                                        >  1:344832/1‑140 (MQ=255)

   tCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACgg                                                                                                                                        >  1:958741/1‑140 (MQ=255)

    cACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGc                                                                                                                                       >  1:1228647/1‑140 (MQ=255)

         gCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTAc                                                                                                                                  >  2:1305209/1‑140 (MQ=255)

                     aCGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGAt                                                                                                                      <  2:1505671/140‑1 (MQ=255)

                           aTCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGaa                                                                                                                >  2:1218230/1‑140 (MQ=255)

                                cGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGcac                                                                                                           <  2:84246/140‑1 (MQ=255)

                                  gACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACaa                                                                                                         >  2:567920/1‑140 (MQ=255)

                                   accaaccaGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAg                                                                                                        <  1:1045938/140‑1 (MQ=255)

                                               gTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGt                                                                                            <  2:1405394/140‑1 (MQ=255)

                                                      aagGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGAt                                                                                     >  1:1423271/1‑140 (MQ=255)

                                                          ttactttcGCGCCGAGCATCTTACCGTGACGCACTGCGCGACGCATGATACGACGCAGTACATAACCACGGTTTTCATTGGTCGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTAcg                                                                                 <  2:1066243/137‑1 (MQ=255)

                                                              tttcGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCTCGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCa                                                                             <  2:1289183/140‑1 (MQ=255)

                                                              tttcGCGCCGAGCAAATTACCGAGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGAACAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCa                                                                             <  2:278937/140‑1 (MQ=255)

                                                              agtcGCGCCGAGCACATTACCGTGACGCACTGCGCGACGACTGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCa                                                                             <  2:1288765/138‑1 (MQ=255)

                                                                 cgcgCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCg                                                                          <  1:336491/140‑1 (MQ=255)

                                                                 cgcgCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCg                                                                          <  2:381965/140‑1 (MQ=255)

                                                                 cgcgCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCg                                                                          <  1:1436201/140‑1 (MQ=255)

                                                                 cgcgCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCg                                                                          <  1:1612163/140‑1 (MQ=255)

                                                                 cgcgCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCg                                                                          <  1:1499129/140‑1 (MQ=255)

                                                                 cgcgCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAAACACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCg                                                                          <  1:705905/140‑1 (MQ=255)

                                                                   cgcCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGAt                                                                        >  1:767891/1‑140 (MQ=255)

                                                                   cgcCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTATCATTGGACGGCATTACGCCATCCGCGATCAGTAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGAt                                                                        >  1:646222/1‑140 (MQ=255)

                                                                      cGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTa                                                                     <  1:1351752/140‑1 (MQ=255)

                                                                           atatTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACTGCATTACGCCATCCGCGATCAGGTACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCt                                                                <  2:337097/140‑1 (MQ=255)

                                                                           atatTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACTGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCt                                                                <  2:1275734/140‑1 (MQ=255)

                                                                           atatTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACTGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCt                                                                <  2:336877/140‑1 (MQ=255)

                                                                                  cGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCg                                                         >  1:1007296/1‑140 (MQ=255)

                                                                                  cGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCg                                                         >  1:1551831/1‑140 (MQ=255)

                                                                                  cGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCAACCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCg                                                         >  1:876668/1‑140 (MQ=255)

                                                                                    tGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGt                                                       >  1:75435/1‑140 (MQ=255)

                                                                                    tGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGt                                                       >  1:77969/1‑140 (MQ=255)

                                                                                    tGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGt                                                       >  1:77704/1‑140 (MQ=255)

                                                                                    tGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGt                                                       >  1:74998/1‑140 (MQ=255)

                                                                                    tGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGt                                                       >  1:655720/1‑140 (MQ=255)

                                                                                    tGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGt                                                       >  2:217381/1‑140 (MQ=255)

                                                                                              cgcgACGAATGATACGACGCAGTACATACCCCCGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGCAACGCACAAGACCGCATGTGGTCAGCGATTACGCGCAGCGAGTTATGGCTCAGATCGGTAGCGCCAGtc                                             >  2:1650363/1‑139 (MQ=255)

                                                                                              cgcgACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTg                                             >  2:1608175/1‑140 (MQ=255)

                                                                                                  acgaATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACtt                                         <  2:267934/140‑1 (MQ=255)

                                                                                                   cgaATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACttt                                        >  1:61022/1‑140 (MQ=255)

                                                                                                       tGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCt                                    <  2:912602/140‑1 (MQ=255)

                                                                                                       tGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCt                                    <  2:912937/140‑1 (MQ=255)

                                                                                                       tGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCt                                    <  2:262482/140‑1 (MQ=255)

                                                                                                        gATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTa                                   <  1:1040990/140‑1 (MQ=255)

                                                                                                        gATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTa                                   <  1:809347/140‑1 (MQ=255)

                                                                                                         aTACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTAc                                  >  2:410815/1‑140 (MQ=255)

                                                                                                               cgCAGTACATAACCACGGTTTTCATTGAACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGcc                                                 >  2:112973/1‑119 (MQ=255)

                                                                                                               cgCAGTACATAACCACGGTTTTCATTGAACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGcc                                                 >  2:112275/1‑119 (MQ=255)

                                                                                                               cgCAGTACATAACCACGGTTTTCATTGAACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGcc                                                 >  2:112254/1‑119 (MQ=255)

                                                                                                               cgCAGTACATAACCACGGTTTTCATTGAACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGcc                                                 <  1:112254/119‑1 (MQ=255)

                                                                                                               cgCAGTACATAACCACGGTTTTCATTGAACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGcc                                                 <  1:112275/119‑1 (MQ=255)

                                                                                                               cgCAGTACATAACCACGGTTTTCATTGAACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGcc                                                 <  1:112973/119‑1 (MQ=255)

                                                                                                                  aGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGAt                         >  1:1078513/1‑140 (MQ=255)

                                                                                                                  aGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGAt                         <  2:578491/140‑1 (MQ=255)

                                                                                                                    tACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCa                       <  2:1106281/140‑1 (MQ=255)

                                                                                                                         aaCCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTg                  >  2:1221589/1‑140 (MQ=255)

                                                                                                                         aaCCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTg                  >  1:1438058/1‑140 (MQ=255)

                                                                                                                         aaCCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTg                  <  1:1691535/140‑1 (MQ=255)

                                                                                                                         aaCCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTg                  >  2:233712/1‑140 (MQ=255)

                                                                                                                         aaCCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTAAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTg                  >  2:964680/1‑140 (MQ=255)

                                                                                                                            cACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCgg               >  2:1087900/1‑140 (MQ=255)

                                                                                                                             aCGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGa              <  1:131471/140‑1 (MQ=255)

                                                                                                                             aCGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGa              <  1:131522/140‑1 (MQ=255)

                                                                                                                             aCGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGa              <  1:131631/140‑1 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACTAATGTGGTCAGCTATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:1293948/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:1424369/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:113314/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:957421/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             <  2:1054704/140‑1 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:542797/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:483410/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:69782/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:1660331/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:67008/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:206749/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             <  2:214715/140‑1 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:69792/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:310456/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:310261/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:277381/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:1469298/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:1431914/1‑140 (MQ=255)

                                                                                                                              cGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATAGGTTGCGCCAGTGACATTCGCTACCGCCTGGATCAGCGTGCGGaa             >  1:69813/1‑140 (MQ=255)

                                                                                                                              cGGGTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGaa             >  2:133121/1‑140 (MQ=255)

                                                                                                                                   ttCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGt        <  2:503533/140‑1 (MQ=255)

                                                                                                                                     cATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGTCg      <  1:638943/140‑1 (MQ=255)

                                                                                                                                     cATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGTCg      <  1:638972/140‑1 (MQ=255)

                                                                                                                                         ggACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGTCGATGt  >  2:695131/1‑140 (MQ=255)

                                                                                                                                         ggACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGTCGATGt  >  2:695174/1‑140 (MQ=255)

TCTTCACCCGCAGAGCCCATAACGTCGATCAGCGGACCAACCAGTTTGTAGAAGAAGGTTTCTTTCGCGCCGAGCATATTACCGTGACGCACTGCGCGACGAATGATACGACGCAGTACATAACCACGGTTTTCATTGGACGGCATTACGCCATCCGCGATCAGGAACGCACAAGAACGAATGTGGTCAGCGATTACGCGCAGCGATTTATTGCTCAGATCGGTTGCGCCAGTGACTTTCGCTACCGCCTGGATCAGCGTGCGGAACAGGTCGATGT  >  NC_000913/2820969‑2821245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`