Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,921,444 G→T 5.9% A219A (GCC→GCA gudP ← putative D‑glucarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,921,4440GT 5.9% 236.5 / 2.2 68A219A (GCC→GCAgudPputative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base G (41/23);  new base T (2/2);  total (43/25)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GTTGATACAGTACTGACCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTC  >  NC_000913/2921306‑2921582
                                                                                                                                          |                                                                                                                                          
gTTGATACAGTACTGACCGATATAACCGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCgcgg                                                                                                                                           <  2:534756/140‑1 (MQ=255)
gTTGATACAGTACTGACCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCTGCGCACCACCCgcgg                                                                                                                                           <  2:534616/140‑1 (MQ=255)
     tACAGTACTGACCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATg                                                                                                                                      <  1:578205/140‑1 (MQ=255)
               aCCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGTCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGct                                                                                                                            >  1:1500647/1‑140 (MQ=255)
               aCCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGct                                                                                                                            >  2:1397397/1‑140 (MQ=255)
               aCCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGct                                                                                                                            >  2:367420/1‑140 (MQ=255)
               aCCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGct                                                                                                                            >  2:367729/1‑140 (MQ=255)
                   atatAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTTCTCCAGCtcttt                                                                                                                        <  2:1046823/140‑1 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:131447/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  2:898849/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  2:330702/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  2:1458554/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:997950/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:811436/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:411158/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:47376/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:51579/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCtctttc                                                                                                                       >  1:1024990/1‑140 (MQ=255)
                    tataAACGCCGATCATCATCCGTGACCATAGCAGCTGTTAGATCTTCCCCCACTTCACGATGAACGGCAATATAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGAACTCCAGCtctttc                                                                                                                       >  1:338892/1‑140 (MQ=255)
                         aCGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTAtt                                                                                                                  <  1:1134826/140‑1 (MQ=255)
                         aCGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTAtt                                                                                                                  <  1:778374/140‑1 (MQ=255)
                         aCGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTAtt                                                                                                                  <  1:765346/140‑1 (MQ=255)
                           gCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTAATTTa                                                                                                                >  2:701785/1‑140 (MQ=255)
                           gCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTAAACTTTGGTTTTTTGCTGATCCATAATGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTATCTTAtttc                                                                                                                >  2:701908/1‑139 (MQ=255)
                                      tcCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGt                                                                                                     >  1:995357/1‑140 (MQ=255)
                                               cTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGc                                                                                            <  2:772595/140‑1 (MQ=255)
                                               cTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGc                                                                                            <  2:772584/140‑1 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCTGCGATGTACTCCAg                                                                                                                              <  2:1612824/104‑1 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCTGCGATGTACTCCAg                                                                                                                              <  2:1612898/104‑1 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCTGCGATGTACTCCAg                                                                                                                              >  1:1612898/1‑104 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc                                                                                          >  1:1141614/1‑140 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc                                                                                          >  2:253706/1‑140 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTACAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc                                                                                          >  1:945799/1‑140 (MQ=255)
                                                 agcagcTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTATTGCTGATCCATATTGATCAGCGCACCACCCGCTGCGATGTACTCCAg                                                                                                                              >  1:1612824/1‑104 (MQ=255)
                                                      cTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGa                                                                                     <  1:880030/140‑1 (MQ=255)
                                                      cTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGa                                                                                     <  1:880158/140‑1 (MQ=255)
                                                      cTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGa                                                                                     <  1:1507930/140‑1 (MQ=255)
                                                                tGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCaa                                                                           <  2:476607/140‑1 (MQ=255)
                                                                tGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCaa                                                                           <  2:906636/140‑1 (MQ=255)
                                                                                  aaCGGCTCTTTAACTTTGTTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGAc                                                         <  2:925026/140‑1 (MQ=255)
                                                                                  aaCGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGAc                                                         <  1:1360450/140‑1 (MQ=255)
                                                                                  aaCGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGAc                                                         >  1:1357518/1‑140 (MQ=255)
                                                                                   aCGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACa                                                        <  1:545255/140‑1 (MQ=255)
                                                                                   aCGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACa                                                        <  1:570118/140‑1 (MQ=255)
                                                                                          tttAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGCTGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTGGATTTGGCTCGTGGTTGACTTTCAACCAGATAAAGCTGATGACAATCCCCa                                                 >  2:450507/1‑140 (MQ=255)
                                                                                            tAACTTTGGTGATTTGCTGATCCATATAGATCAGCGCACCACCCGCGGCGATGTACACAAGCTCTTTATTATTTACCACCGGATGATGATAAGGCTCGTGGATGACTTTCAACCAGAAAAAGCTGATGACAATCCCCAGa                                               >  1:1422026/1‑140 (MQ=255)
                                                                                                 ttGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGcc                                          >  1:1614251/1‑140 (MQ=255)
                                                                                                    gtgtTTTGCTGATCCATATTGATCCGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCACGTGGATGACTTTCAACCAGATACAGCTGATGACCATCCCCAGACCGCCCAt                                       >  2:1171144/1‑140 (MQ=255)
                                                                                                    gtgtTTTGCTGATCCATATTGATCAGCGCTCCACCCGCGGAGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCAt                                       <  2:649416/140‑1 (MQ=255)
                                                                                                    gtgtTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCAt                                       >  2:1259145/1‑140 (MQ=255)
                                                                                                    gtgtTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCAt                                       >  2:1190049/1‑140 (MQ=255)
                                                                                                    gtgtTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCACTTTCTTATTTACCCCCGGAGGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCaa                                       >  1:342583/1‑139 (MQ=255)
                                                                                                     tgtTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATa                                      <  2:1578289/140‑1 (MQ=255)
                                                                                                             tGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAaagaagaa                              <  2:1550468/140‑1 (MQ=255)
                                                                                                               aTCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAagaagaaga                            >  1:679706/1‑140 (MQ=255)
                                                                                                                      ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATATAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGa                     <  2:432772/140‑1 (MQ=255)
                                                                                                                         aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa                  >  1:1535288/1‑140 (MQ=255)
                                                                                                                         aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTGCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGTTGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa                  >  1:722739/1‑140 (MQ=255)
                                                                                                                         aTCAGCGCACCACCCGCGGCGATGTACTACAGCTCTTTCTTATTTACCCCCGGAGGGTGATTTGGCTCTTGGATGTCTTTAAAACTGTATAATCTGATGACAATACCCAGAGCGCCCATAAAGAAGAAGACGTGTGACCa                  >  2:1670553/1‑140 (MQ=255)
                                                                                                                            aGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGcc               >  2:829654/1‑140 (MQ=255)
                                                                                                                            aGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGcc               <  2:383034/140‑1 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGAt                                                                                    >  2:290817/1‑69 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGAt                                                                                    <  1:290817/69‑1 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGTCGTGTGACCAGCCCa             >  1:751476/1‑140 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCa             >  1:71091/1‑140 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCa             >  2:734484/1‑140 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCa             >  2:734582/1‑140 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCa             >  1:751605/1‑140 (MQ=255)
                                                                                                                              cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAAAAGAAGACGTGTGACCAGCCCa             >  1:812779/1‑140 (MQ=255)
                                                                                                                                 accaccCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATGTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACtt          <  2:388239/140‑1 (MQ=255)
                                                                                                                                        gcggcgATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGt   >  1:980214/1‑140 (MQ=255)
                                                                                                                                        gcggcgATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGt   >  1:1249387/1‑140 (MQ=255)
                                                                                                                                        gcggcgATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGt   >  2:33629/1‑140 (MQ=255)
                                                                                                                                         cggcgATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGTTAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTc  >  2:890781/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GTTGATACAGTACTGACCGATATAAACGCCGATCATCATCCGTGACCCTAGCAGCTGTTTGATCTGCCCCCACTTCACGCTGAACGGCACTTTAACTTTGGTGTTTTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTC  >  NC_000913/2921306‑2921582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: