Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,157,433 G→T 5.9% D271Y (GAC→TAC)  dkgA → 2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,157,4330GT 5.9% 246.4 / 2.1 68D271Y (GAC→TAC) dkgA2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base G (28/36);  new base T (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGGCCTGGTGGTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATC  >  NC_000913/3157294‑3157572
                                                                                                                                           |                                                                                                                                           
cGGCCTGGTGGTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTg                                                                                                                                             >  1:1529065/1‑140 (MQ=255)
  gCCTGGTGGTGATCCCGAAATCGGTAACACATACACGTATTGCCGAAAACATTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGAc                                                                                                                                           <  2:163227/140‑1 (MQ=255)
     tggtggTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAAGTGCAAAACGCTATCAGGGCAAGCGTCTCGGTCCCGTTCCTGACCAg                                                                                                                                        >  2:873390/1‑140 (MQ=255)
     tggtggTGATCCCGAAATCGGTAACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGATCTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAg                                                                                                                                        >  1:1578021/1‑140 (MQ=255)
     tgcttgtgATCCAGAAATCGCTCACACCTTCACGTATTGCCGAAAACTTTTATGTCTGGAATTTCCGACTCGACAAAGACGTACTGGTCGATATAGCATAACACGATCAGGGCAAGCTTATCTGTGACGAACCTGACCAg                                                                                                                                        >  2:856852/6‑140 (MQ=255)
          gtgATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGAGGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcgg                                                                                                                                   >  2:561501/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  1:312457/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  1:1506499/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  2:642459/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  2:594042/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  1:537498/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  2:385381/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  1:1017508/1‑140 (MQ=255)
             aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                >  1:1017575/1‑140 (MQ=255)
               cccGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCt                                                                                                                              <  1:1326530/140‑1 (MQ=255)
                 cGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTaa                                                                                                                            <  1:935681/140‑1 (MQ=255)
                 cGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTaa                                                                                                                            >  2:779806/1‑140 (MQ=255)
                         gTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCaaa                                                                                                                    >  1:703373/1‑140 (MQ=255)
                                  tCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCgg                                                                                                           >  2:510754/1‑140 (MQ=255)
                                  tCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCgg                                                                                                           >  2:511112/1‑140 (MQ=255)
                                     cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTgg                                                                                                        <  1:1649571/140‑1 (MQ=255)
                                           gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCTAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                  >  2:1605836/1‑140 (MQ=255)
                                           gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                  >  2:1073845/1‑140 (MQ=255)
                                           gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                  >  2:761269/1‑140 (MQ=255)
                                           gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                  >  2:897097/1‑140 (MQ=255)
                                                aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                             <  1:799314/140‑1 (MQ=255)
                                                aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                             <  1:1394985/140‑1 (MQ=255)
                                                      tGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTAc                                                                                       >  2:348572/1‑140 (MQ=255)
                                                        ctGTCTGGGATTTCCGTATCGACAAAGTAGAACTCGGCGAAATTGCAAAACTCGATCTGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGGTCCGCTACCg                                                                                     <  1:1638109/139‑1 (MQ=255)
                                                        aTGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCg                                                                                     <  1:1623329/140‑1 (MQ=255)
                                                                  tttCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAg                                                                           <  1:149123/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         <  2:587989/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         <  2:590040/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         <  1:1502140/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         <  2:869291/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         <  1:1616609/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         <  1:187632/140‑1 (MQ=255)
                                                                    tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                         >  1:1075012/1‑140 (MQ=255)
                                                                       gTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATc                                                                      <  2:504615/140‑1 (MQ=255)
                                                                          tcGACAAAGTCGATCTCTGCGAAAGTGCAAAACTCGTTCAGGGCAAGCGTCTCGGTCCCGATCCTGATCTGTTCGGCGGCTAACATGCATTTTCTCCCGGTGGAGGTAATGTTCCGCTACCTGACTTTTCAGAATTCAtt                                                                   <  2:861216/140‑1 (MQ=255)
                                                                          tcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTACCTTGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGATATCAtt                                                                   <  2:949482/140‑1 (MQ=255)
                                                                          tcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCAtt                                                                   <  2:1448547/140‑1 (MQ=255)
                                                                                 aGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTccc                                                            <  1:836860/140‑1 (MQ=255)
                                                                                                 aTTGCAAACCTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCgt                                            <  1:1094846/140‑1 (MQ=255)
                                                                                                             gATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTcc                                <  1:48257/140‑1 (MQ=255)
                                                                                                                    gCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTGGTTACTCTTCCTTGTTCa                         >  2:1478418/1‑140 (MQ=255)
                                                                                                                       aGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                      <  1:510754/140‑1 (MQ=255)
                                                                                                                       aGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                      <  1:511112/140‑1 (MQ=255)
                                                                                                                       aGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                      <  1:1677898/140‑1 (MQ=255)
                                                                                                                       aGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                      <  1:1678073/140‑1 (MQ=255)
                                                                                                                       aGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                      <  1:1194753/140‑1 (MQ=255)
                                                                                                                          gTCTCGGTCCCGTTCCTGACCAGTTCGGCGGCTAACATGCAATTTCTCCCGGTGGCGGTATTGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGGGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATg                   <  2:1082534/140‑1 (MQ=255)
                                                                                                                          gTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGTCTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATg                   <  2:555692/140‑1 (MQ=255)
                                                                                                                             tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                >  1:1309234/1‑140 (MQ=255)
                                                                                                                             tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                >  1:1327018/1‑140 (MQ=255)
                                                                                                                             tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                >  1:333168/1‑140 (MQ=255)
                                                                                                                             tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                >  1:566607/1‑140 (MQ=255)
                                                                                                                             tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                >  1:567077/1‑140 (MQ=255)
                                                                                                                             tcGGTCCAGATCCTGAGGAGTTCGGCGGCTAAGTTTCAAATTCTCCCGGTGGCGGTAATGTTGCGCTACCGGACTTTTCAGAAATCTTTTATTCCCCTCGCGTCCCGCGCGTTGTTACTCTTCATTGTTCAGGAATGCCa                <  2:419864/140‑1 (MQ=255)
                                                                                                                                gTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAat             >  1:823915/1‑140 (MQ=255)
                                                                                                                                  cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat           <  2:409914/140‑1 (MQ=255)
                                                                                                                                  cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat           <  2:857891/140‑1 (MQ=255)
                                                                                                                                  cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat           <  2:858036/140‑1 (MQ=255)
                                                                                                                                  cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat           <  2:312457/140‑1 (MQ=255)
                                                                                                                                   ccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTGCTGGACTGCCAAAtata          >  1:1604819/1‑140 (MQ=255)
                                                                                                                                       tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa      <  1:1270876/140‑1 (MQ=255)
                                                                                                                                       tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa      <  2:686700/140‑1 (MQ=255)
                                                                                                                                       tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa      <  1:837164/140‑1 (MQ=255)
                                                                                                                                       tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa      <  1:836486/140‑1 (MQ=255)
                                                                                                                                           gACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCTGAAATCATTTATTCCCCTCGCGTCCCGCCCGTGGTTACTCTTCCTTGTTCAGGAATGCCAATTATAAGGAcatc  >  2:9035/1‑140 (MQ=255)
                                                                                                                                           gACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAATTATAAGGAcatc  >  2:8894/1‑140 (MQ=255)
                                                                                                                                           gACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGAcatc  >  2:1171371/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGGCCTGGTGGTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATC  >  NC_000913/3157294‑3157572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑