Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,157,442	G→T	6.5%	G274C (GGC→TGC)	dkgA →	2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,442	0	G	T	6.5%	220.1 / 2.3	61	G274C (GGC→TGC)	dkgA	2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base G (22/35);  new base T (2/2);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATG  >  NC_000913/3157304‑3157578                                                                                                                                           |                                                                                                                                         gtgATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGAGGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcgg                                                                                                                                         >  2:561501/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  1:1017575/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  2:385381/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  1:537498/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  1:312457/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  2:594042/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  2:642459/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  1:1506499/1‑140 (MQ=255)    aTCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTcggcgg                                                                                                                                      >  1:1017508/1‑140 (MQ=255)      cccGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCt                                                                                                                                    <  1:1326530/140‑1 (MQ=255)        cGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTaa                                                                                                                                  <  1:935681/140‑1 (MQ=255)        cGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTaa                                                                                                                                  >  2:779806/1‑140 (MQ=255)                gTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCaaa                                                                                                                          >  1:703373/1‑140 (MQ=255)                         tCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCgg                                                                                                                 >  2:510754/1‑140 (MQ=255)                         tCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCgg                                                                                                                 >  2:511112/1‑140 (MQ=255)                            cGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTgg                                                                                                              <  1:1649571/140‑1 (MQ=255)                                  gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCTAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                        >  2:1605836/1‑140 (MQ=255)                                  gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                        >  2:897097/1‑140 (MQ=255)                                  gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                        >  2:1073845/1‑140 (MQ=255)                                  gCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTaa                                                                                                        >  2:761269/1‑140 (MQ=255)                                       aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                                   <  1:1394985/140‑1 (MQ=255)                                       aaaCTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTc                                                                                                   <  1:799314/140‑1 (MQ=255)                                             tGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTAc                                                                                             >  2:348572/1‑140 (MQ=255)                                               ctGTCTGGGATTTCCGTATCGACAAAGTAGAACTCGGCGAAATTGCAAAACTCGATCTGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGGTCCGCTACCg                                                                                           <  1:1638109/139‑1 (MQ=255)                                               aTGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCg                                                                                           <  1:1623329/140‑1 (MQ=255)                                                         tttCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAg                                                                                 <  1:149123/140‑1 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               <  1:187632/140‑1 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               <  1:1502140/140‑1 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               >  1:1075012/1‑140 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               <  1:1616609/140‑1 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               <  2:587989/140‑1 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               <  2:590040/140‑1 (MQ=255)                                                           tCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGaa                                                                               <  2:869291/140‑1 (MQ=255)                                                              gTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATc                                                                            <  2:504615/140‑1 (MQ=255)                                                                 tcGACAAAGTCGATCTCTGCGAAAGTGCAAAACTCGTTCAGGGCAAGCGTCTCGGTCCCGATCCTGATCTGTTCGGCGGCTAACATGCATTTTCTCCCGGTGGAGGTAATGTTCCGCTACCTGACTTTTCAGAATTCAtt                                                                         <  2:861216/140‑1 (MQ=255)                                                                 tcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTACCTTGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGATATCAtt                                                                         <  2:949482/140‑1 (MQ=255)                                                                 tcGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCAtt                                                                         <  2:1448547/140‑1 (MQ=255)                                                                        aGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTccc                                                                  <  1:836860/140‑1 (MQ=255)                                                                                        aTTGCAAACCTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCgt                                                  <  1:1094846/140‑1 (MQ=255)                                                                                                    gATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTcc                                      <  1:48257/140‑1 (MQ=255)                                                                                                           gCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTGGTTACTCTTCCTTGTTCa                               >  2:1478418/1‑140 (MQ=255)                                                                                                              aGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                            <  1:510754/140‑1 (MQ=255)                                                                                                              aGCGTCTCGGTCCCGATCCTTACCAGTTCTGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                            <  1:511112/140‑1 (MQ=255)                                                                                                              aGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                            <  1:1194753/140‑1 (MQ=255)                                                                                                              aGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                            <  1:1677898/140‑1 (MQ=255)                                                                                                              aGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGa                            <  1:1678073/140‑1 (MQ=255)                                                                                                                 gTCTCGGTCCCGTTCCTGACCAGTTCGGCGGCTAACATGCAATTTCTCCCGGTGGCGGTATTGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGGGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATg                         <  2:1082534/140‑1 (MQ=255)                                                                                                                 gTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGTCTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATg                         <  2:555692/140‑1 (MQ=255)                                                                                                                    tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                      >  1:566607/1‑140 (MQ=255)                                                                                                                    tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                      >  1:567077/1‑140 (MQ=255)                                                                                                                    tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                      >  1:333168/1‑140 (MQ=255)                                                                                                                    tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                      >  1:1309234/1‑140 (MQ=255)                                                                                                                    tcGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCa                      >  1:1327018/1‑140 (MQ=255)                                                                                                                    tcGGTCCAGATCCTGAGGAGTTCGGCGGCTAAGTTTCAAATTCTCCCGGTGGCGGTAATGTTGCGCTACCGGACTTTTCAGAAATCTTTTATTCCCCTCGCGTCCCGCGCGTTGTTACTCTTCATTGTTCAGGAATGCCa                      <  2:419864/140‑1 (MQ=255)                                                                                                                       gTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAat                   >  1:823915/1‑140 (MQ=255)                                                                                                                         cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat                 <  2:312457/140‑1 (MQ=255)                                                                                                                         cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat                 <  2:409914/140‑1 (MQ=255)                                                                                                                         cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat                 <  2:858036/140‑1 (MQ=255)                                                                                                                         cccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAatat                 <  2:857891/140‑1 (MQ=255)                                                                                                                          ccGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTGCTGGACTGCCAAAtata                >  1:1604819/1‑140 (MQ=255)                                                                                                                              tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa            <  1:837164/140‑1 (MQ=255)                                                                                                                              tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa            <  1:836486/140‑1 (MQ=255)                                                                                                                              tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa            <  1:1270876/140‑1 (MQ=255)                                                                                                                              tCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGa            <  2:686700/140‑1 (MQ=255)                                                                                                                                  gACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCTGAAATCATTTATTCCCCTCGCGTCCCGCCCGTGGTTACTCTTCCTTGTTCAGGAATGCCAATTATAAGGAcatc        >  2:9035/1‑140 (MQ=255)                                                                                                                                  gACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAATTATAAGGAcatc        >  2:8894/1‑140 (MQ=255)                                                                                                                                  gACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGAcatc        >  2:1171371/1‑140 (MQ=255)                                                                                                                                       gTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGAcatcatcat   >  1:407130/1‑140 (MQ=255)                                                                                                                                       gTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGAcatcatcat   >  1:575220/1‑140 (MQ=255)                                                                                                                                        ttCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATg  >  1:298833/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                         GTGATCCCGAAATCGGTCACACCTTCACGTATTGCCGAAAACTTTGATGTCTGGGATTTCCGTCTCGACAAAGACGAACTCGGCGAAATTGCAAAACTCGATCAGGGCAAGCGTCTCGGTCCCGATCCTGACCAGTTCGGCGGCTAACATGCAAATTCTCCCGGTGGCGGTAATGTTCCGCTACCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATG  >  NC_000913/3157304‑3157578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑