Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,204,386 A→C 6.3% R68R (CGT→CGG folB ← dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,204,3860AC 6.3% 266.3 / 8.8 96R68R (CGT→CGGfolBdihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (35/54);  new base C (2/4);  total (38/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

CGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCT  >  NC_000913/3204249‑3204525
                                                                                                                                         |                                                                                                                                           
cGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcg                                                                                                                                           >  1:1327017/1‑140 (MQ=255)
  cTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcgcg                                                                                                                                         <  2:291070/140‑1 (MQ=255)
  cTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcgcg                                                                                                                                         <  2:10053/140‑1 (MQ=255)
  cTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcgcg                                                                                                                                         <  1:1011793/140‑1 (MQ=255)
  cTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcgcg                                                                                                                                         >  2:1420740/1‑140 (MQ=255)
  cTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcgcg                                                                                                                                         <  2:1071937/140‑1 (MQ=255)
  cTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAAcgcg                                                                                                                                         <  1:1268474/140‑1 (MQ=255)
  cTCAATGATTACGCAAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTACACCAGCGCAAAAcgcg                                                                                                                                         <  2:1003314/140‑1 (MQ=255)
  cTCAATGATTACGCAAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTACACCAGCGCAAAAcgcg                                                                                                                                         <  2:25774/140‑1 (MQ=255)
  cTCAATGATTACGCAAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTACACCAGCGCAAAAcgcg                                                                                                                                         <  2:129530/140‑1 (MQ=255)
   tCACTGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACgcgc                                                                                                                                        <  1:409584/140‑1 (MQ=255)
      ctGAGTACGCCCACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGcccc                                                                                                                                     <  1:1656659/139‑1 (MQ=255)
      aTGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGcccc                                                                                                                                     <  1:995240/140‑1 (MQ=255)
      aTGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGcccc                                                                                                                                     <  1:995269/140‑1 (MQ=255)
           tACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGa                                                                                                                                >  2:659909/1‑140 (MQ=255)
           tACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGa                                                                                                                                >  2:660047/1‑140 (MQ=255)
             cGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACg                                                                                                                              >  1:840447/1‑140 (MQ=255)
             cGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACg                                                                                                                              >  1:1519006/1‑140 (MQ=255)
             cGCCAACATTCGCCGCCCGCGCCACTGCGCCGGGCTTGCAGAGAGTGATACGCACCCACGGCGAGTTGAAGCGGGCTCACAGCAGCACCGCCACCGCTTCAGCCACGCGAGCCACCACCGCCAAACGCGCCCCCacgacg                                                                                                                              >  2:1639668/1‑140 (MQ=255)
               ccAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTg                                                                                                                            >  1:52966/1‑140 (MQ=255)
                  aCATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCt                                                                                                                         >  2:1550991/1‑140 (MQ=255)
                  aCATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCt                                                                                                                         <  2:626299/140‑1 (MQ=255)
                      tCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCGACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGacc                                                                                                                     <  2:878171/140‑1 (MQ=255)
                      tCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGacc                                                                                                                     <  1:1054682/140‑1 (MQ=255)
                        gccgccCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGaccac                                                                                                                   >  2:372276/1‑140 (MQ=255)
                          cgcccgcGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCg                                                                                                                 >  1:1264651/1‑140 (MQ=255)
                          cgcccgcGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCg                                                                                                                 >  2:885236/1‑140 (MQ=255)
                          cgcccgcGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCg                                                                                                                 >  1:1264659/1‑140 (MQ=255)
                          cgcccgcGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTACAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCg                                                                                                                 <  1:1529159/140‑1 (MQ=255)
                              cgcgCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGAGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGCCTGACCACCGTTTc                                                                                                             >  2:1334129/1‑140 (MQ=255)
                                    aCTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAAt                                                                                                       <  1:162164/140‑1 (MQ=255)
                                         gcCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAg                                                                                                  <  1:634698/140‑1 (MQ=255)
                                          ccTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGc                                                                                                 >  1:661259/1‑140 (MQ=255)
                                                  gCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGa                                                                                         >  1:818042/1‑140 (MQ=255)
                                                        tttGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAATCGCGCCCCCTCGTCGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCTAt                                                                                   >  1:748571/1‑140 (MQ=255)
                                                            aTACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGc                                                                               <  2:1160642/140‑1 (MQ=255)
                                                            aTACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGc                                                                               <  2:380255/140‑1 (MQ=255)
                                                            aTACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGc                                                                               <  1:1481245/140‑1 (MQ=255)
                                                            aTACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGc                                                                               <  2:1438817/140‑1 (MQ=255)
                                                            aTACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGc                                                                               <  2:1436899/140‑1 (MQ=255)
                                                            aTACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTACAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGc                                                                               <  2:896848/140‑1 (MQ=255)
                                                                  aCCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCAcatc                                                                         >  1:123624/1‑140 (MQ=255)
                                                                  aCCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCAcatc                                                                         >  1:123303/1‑140 (MQ=255)
                                                                  aCCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCGCGACGTGGCAGACCACCGTTTCTGCAAGGTCAGCGTAACTGAGGCACTCCGCCAcatc                                                                         >  2:683820/1‑140 (MQ=255)
                                                                            aGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTc                                                               >  1:1050625/1‑140 (MQ=255)
                                                                            aGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTc                                                               >  1:1047436/1‑140 (MQ=255)
                                                                             gTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTcg                                                              <  1:269045/140‑1 (MQ=255)
                                                                             gTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTcg                                                              <  2:861092/140‑1 (MQ=255)
                                                                              ttGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTcgc                                                             <  2:788992/140‑1 (MQ=255)
                                                                              ttGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTcgc                                                             <  1:1167480/140‑1 (MQ=255)
                                                                                   gCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCtt                                                        <  1:135210/140‑1 (MQ=255)
                                                                                      tGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTAc                                                     <  1:716679/140‑1 (MQ=255)
                                                                                        cTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACgg                                                   >  2:831091/1‑140 (MQ=255)
                                                                                         tAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGt                                                  <  2:1259909/140‑1 (MQ=255)
                                                                                         tAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGt                                                  <  1:113678/140‑1 (MQ=255)
                                                                                         tAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGt                                                  <  1:113196/140‑1 (MQ=255)
                                                                                              gcagcTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATcc                                             >  1:1309785/1‑140 (MQ=255)
                                                                                                  cTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACg                                         >  1:939437/1‑140 (MQ=255)
                                                                                                   tCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGc                                        <  2:1420570/140‑1 (MQ=255)
                                                                                                   tCCGCCACCTCTGCACCCACGCGTACCACCAGCGCACTCCGCGCCCCCACGACGTGGCTGACCACCGTCTCTGCCAAGTCACCGTCCCTGAGGCACACCGCCACAACATCACCTTTCGCCGCTCTCCGGCTATCCCACGc                                        <  2:645119/140‑1 (MQ=255)
                                                                                                    ccGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGcc                                       <  1:1295320/140‑1 (MQ=255)
                                                                                                    ccGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGcc                                       <  2:1294507/140‑1 (MQ=255)
                                                                                                          ccTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCg                                 <  2:657856/140‑1 (MQ=255)
                                                                                                          ccTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCg                                 <  2:161759/140‑1 (MQ=255)
                                                                                                               tCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATc                            >  2:752527/1‑140 (MQ=255)
                                                                                                                cAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCg                           <  2:1309785/140‑1 (MQ=255)
                                                                                                                 aGCCCCGCGTTCCACCAGCGCAAACCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATGCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGa                          <  2:254229/140‑1 (MQ=255)
                                                                                                                 aGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGa                          <  2:675738/140‑1 (MQ=255)
                                                                                                                 aGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGa                          <  2:675733/140‑1 (MQ=255)
                                                                                                                    cACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAaca                       <  2:851594/140‑1 (MQ=255)
                                                                                                                     aCGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAacac                      >  1:167914/1‑140 (MQ=255)
                                                                                                                     aCGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCGTTACGGTTATCCCACGCCATTTCGATATCGAacac                      >  1:168014/1‑140 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAACCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTg                                                                                                           >  1:284832/1‑54 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAACCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTg                                                                                                           <  2:285063/54‑1 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAACCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTg                                                                                                           <  2:284832/54‑1 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAACCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTg                                                                                                           >  1:285063/1‑54 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCTATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGTTATCGAACACt                     <  1:269129/140‑1 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACt                     >  2:439768/1‑140 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACt                     <  1:832351/140‑1 (MQ=255)
                                                                                                                      cgcgTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACt                     >  2:795891/1‑140 (MQ=255)
                                                                                                                         gTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAAc                  <  2:228642/140‑1 (MQ=255)
                                                                                                                         gTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAAc                  <  2:156597/140‑1 (MQ=255)
                                                                                                                                  gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  1:880893/140‑1 (MQ=255)
                                                                                                                                  gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  2:477074/140‑1 (MQ=255)
                                                                                                                                  gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  2:476840/140‑1 (MQ=255)
                                                                                                                                  gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  1:1390498/140‑1 (MQ=255)
                                                                                                                                  gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  1:1420740/140‑1 (MQ=255)
                                                                                                                                  gcgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  2:1327017/140‑1 (MQ=255)
                                                                                                                                  ccgcAAAACGCGCCCCCTCGACGTGGCTGACCCCCGTTACTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCACTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCg         <  1:1684973/139‑1 (MQ=255)
                                                                                                                                   cgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGa        >  1:732482/1‑140 (MQ=255)
                                                                                                                                    gcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGAt       <  2:99883/140‑1 (MQ=255)
                                                                                                                                     cAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATg      >  2:628505/1‑140 (MQ=255)
                                                                                                                                     cAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATg      >  2:628025/1‑140 (MQ=255)
                                                                                                                                     cAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATg      >  2:1035599/1‑140 (MQ=255)
                                                                                                                                     cAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATg      >  2:1034824/1‑140 (MQ=255)
                                                                                                                                     cAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATg      >  2:902244/1‑140 (MQ=255)
                                                                                                                                       aaaCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGt    <  1:287051/140‑1 (MQ=255)
                                                                                                                                       aaaCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGt    <  1:191691/140‑1 (MQ=255)
                                                                                                                                         aCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCt  <  1:660047/140‑1 (MQ=255)
                                                                                                                                         aCGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCt  <  1:659909/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGGCGAGTTGAAGCGTGCTAACAGCAGCTCCGCCACCTCTTCAGCCACGCGTTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCT  >  NC_000913/3204249‑3204525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: