Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,244,970 C→A 6.8% intergenic (‑229/‑254) uxaC ← / → exuT uronate isomerase/hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,244,9700CA 6.8% 267.9 / 4.2 81intergenic (‑229/‑254)uxaC/exuTuronate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (44/30);  new base A (2/4);  total (47/34)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGC  >  NC_000913/3244836‑3245108
                                                                                                                                      |                                                                                                                                          
gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTa                                                                                                                                       <  2:1610751/140‑1 (MQ=255)
 aGTTAGATCACAAAACGAAGTTAAAAAAATTACTCTCAAAGTTGTATATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTaa                                                                                                                                      <  2:812854/140‑1 (MQ=255)
 aGTTAGATCAATAAACGTAGTTAAAAATATTACTCTCAACGTGGTTAATCTCGCTGCACGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTaa                                                                                                                                      <  2:812918/140‑1 (MQ=255)
            tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGAc                                                                                                                           >  1:1186324/1‑140 (MQ=255)
            tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGAc                                                                                                                           >  1:1411668/1‑140 (MQ=255)
             aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACa                                                                                                                          <  1:276938/140‑1 (MQ=255)
                cGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                                                                                                       <  2:1147546/140‑1 (MQ=255)
                                 ctctCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGc                                                                                                      <  1:1375708/140‑1 (MQ=255)
                                       aaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACTAGTTATCTTTCTGCCGTCGCTAATCa                                                                                                <  2:600934/140‑1 (MQ=255)
                                       aaGTGGTAAATCTCGCAGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa                                                                                                <  2:600970/140‑1 (MQ=255)
                                            gTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt                                                                                           >  1:1535515/1‑140 (MQ=255)
                                            gTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt                                                                                           >  1:33149/1‑140 (MQ=255)
                                            gTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt                                                                                           >  1:33092/1‑140 (MQ=255)
                                                aTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc                                                                                       <  1:1583581/140‑1 (MQ=255)
                                                aTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc                                                                                       <  1:12280/140‑1 (MQ=255)
                                                      cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATg                                                                                 >  2:1429002/1‑140 (MQ=255)
                                                      cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATg                                                                                 >  1:404099/1‑140 (MQ=255)
                                                                cgcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGa                                                                                        >  1:870500/1‑123 (MQ=255)
                                                                cgcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGa                                                                                        <  2:870500/123‑1 (MQ=255)
                                                                 gcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCCAATTGCCGa                                                                      >  2:1122829/1‑140 (MQ=255)
                                                                  ccAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGat                                                                     <  2:1317018/140‑1 (MQ=255)
                                                                      tACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTTTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcat                                                                 >  2:774136/1‑140 (MQ=255)
                                                                      tACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcat                                                                 >  1:580254/1‑140 (MQ=255)
                                                                       aCTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcatt                                                                <  1:150021/140‑1 (MQ=255)
                                                                       aCTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcatt                                                                <  1:150020/140‑1 (MQ=255)
                                                                         tGGCCTTGCTGTCGTCAGGTAATGTCCCTTCAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGTCGGAATGCAAATTGCCGAttcattca                                                              <  2:1082863/140‑1 (MQ=255)
                                                                         tGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGAttcattca                                                              <  1:967878/140‑1 (MQ=255)
                                                                         tGGCCTTGCTGTCGTCAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGCTCTCACATTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCAGAttcattca                                                              <  2:319727/140‑1 (MQ=255)
                                                                         tGGCCTTGCTGTCGTCAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGCTCTCACATTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCAGAttcattca                                                              <  2:319709/140‑1 (MQ=255)
                                                                         tGGCCTTGCTGTCGTCAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGCTCTCACATTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCAGAttcattca                                                              <  2:320348/140‑1 (MQ=255)
                                                                         tGGCCTTGCTGTCGTCAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGCTCTCACATTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCAGAttcattca                                                              <  2:319982/140‑1 (MQ=255)
                                                                          ggCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGACTGCAAATTGCCGATtcattcat                                                             >  1:926331/1‑140 (MQ=255)
                                                                           gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCGACATTTGTGATGGCTCTCAGCTTTTTTAGTTGTATGTCAAGTTATCTTTCTGGCGTCGCAAATCATAAGTCGTGGGAATGCAAATTGACGTTTcattcatt                                                            >  2:1057807/1‑140 (MQ=255)
                                                                           gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACGTTTTAAAGTTGTATGACAAGTTATATTTCTGCCGTCGCAAATCATAATTCGACGGAATGCAAATTGCCGATTcattcatt                                                            >  1:302183/1‑140 (MQ=255)
                                                                           gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGACTGCAAATTGCCGAGTcattcatt                                                            >  1:1005328/1‑140 (MQ=255)
                                                                           gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTcattcatt                                                            >  2:1341243/1‑140 (MQ=255)
                                                                           gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACATTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCCAATTGCCGATTcattcatt                                                            >  1:586400/1‑140 (MQ=255)
                                                                           gCCTTGCTGTCGTCAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGATCTCACATTGTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATACGTCGACGGACTGCAAAGTGCCGATTcattcatt                                                            >  2:649454/1‑140 (MQ=255)
                                                                              ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGt                                                         <  2:1357057/140‑1 (MQ=255)
                                                                              ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGt                                                         <  2:1356620/140‑1 (MQ=255)
                                                                                gCTGTCGTCAGGTGATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTGTGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                                                       >  2:841050/1‑140 (MQ=255)
                                                                                gCTGTCGTCAGGTAATGTCCCTACAAATTTTCCCAGATTTGTGATGGCTCTCACCTTTTATAGTTGTTTGTCAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAGGGTATGCATTTTGCCGATTCATTCATTTGttt                                                       >  2:766115/1‑139 (MQ=255)
                                                                                gCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                                                       >  2:939502/1‑140 (MQ=255)
                                                                                gCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                                                       >  1:1565556/1‑140 (MQ=255)
                                                                                gCTGTCGTCAGGTAATGTCCCTACAAATATTCCAACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                                                       >  1:953415/1‑140 (MQ=255)
                                                                                gCTGTCGTCAGGTAATGTCCCTACAAATATTCCAACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATATTTCTGACGTCGCAAATCATAATTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                                                       >  1:953402/1‑140 (MQ=255)
                                                                                      gtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGaa                                                 <  1:1658585/140‑1 (MQ=255)
                                                                                      gtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGaa                                                 >  1:440050/1‑140 (MQ=255)
                                                                                       tcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAAt                                                <  2:336156/140‑1 (MQ=255)
                                                                                       tcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAAt                                                <  2:1000178/140‑1 (MQ=255)
                                                                                       tcAGGTAATGTCCCTACAAATAGTCCCACCTTTGTGATGGCTCTCACCTTTTAAAGTTGTCTGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAAGTGCCGATTCATTCATTTGTTAGATGAAt                                                <  2:635238/140‑1 (MQ=255)
                                                                                           gTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCggg                                            >  1:500999/1‑140 (MQ=255)
                                                                                             aaTGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGtt                                          <  1:1328329/140‑1 (MQ=255)
                                                                                              aTGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTa                                         >  1:450114/1‑140 (MQ=255)
                                                                                              aTGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTa                                         >  2:152497/1‑140 (MQ=255)
                                                                                               tGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTaa                                        <  2:975463/140‑1 (MQ=255)
                                                                                                gTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAAc                                       <  1:702373/140‑1 (MQ=255)
                                                                                                gTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAAc                                       <  1:41186/140‑1 (MQ=255)
                                                                                                gTCCATACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAAc                                       <  2:585148/140‑1 (MQ=255)
                                                                                                   ccTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCgg                                    >  2:891263/1‑140 (MQ=255)
                                                                                                   ccTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCgg                                    >  1:1580508/1‑140 (MQ=255)
                                                                                                   ccTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCgg                                    >  2:891419/1‑140 (MQ=255)
                                                                                                   ccTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCgg                                    >  2:891433/1‑140 (MQ=255)
                                                                                                     tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTa                                  >  1:1683368/1‑140 (MQ=255)
                                                                                                     tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTa                                  >  1:1268374/1‑140 (MQ=255)
                                                                                                     tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTa                                  >  1:1267956/1‑140 (MQ=255)
                                                                                                     tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTa                                  <  2:671226/140‑1 (MQ=255)
                                                                                                          atatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGGTAACCGGTACTGac                             >  2:617326/1‑139 (MQ=255)
                                                                                                           tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGACTGCAAATTGCCGATTCATACATTTGTTAGATGAATCGGGTTAACCGGTACGGAAg                            >  2:806345/1‑140 (MQ=255)
                                                                                                           tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGaa                                                 >  2:1194109/1‑119 (MQ=255)
                                                                                                           tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGaa                                                 <  1:1194109/119‑1 (MQ=255)
                                                                                                           tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAg                            >  2:103346/1‑140 (MQ=255)
                                                                                                           tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATCCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAg                            >  2:860242/1‑140 (MQ=255)
                                                                                                               cccACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGa                        >  1:1162906/1‑140 (MQ=255)
                                                                                                                 cacaTTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAAt                      >  2:165320/1‑140 (MQ=255)
                                                                                                                      ttGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCa                 >  2:379944/1‑140 (MQ=255)
                                                                                                                      ttGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCa                 >  1:625888/1‑140 (MQ=255)
                                                                                                                        gtgATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACg               >  2:338140/1‑140 (MQ=255)
                                                                                                                        gtgATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACg               >  1:1540190/1‑140 (MQ=255)
                                                                                                                         tgATGGCTCTCACCTTTTTAAGTTGTATTACATGTTATCTTTTTTCCGTCGCAAATCATATGTCGACTGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCTGTACGGTAGCCGATTTAGCACGa              <  2:255928/140‑1 (MQ=255)
                                                                                                                           aTGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGaaa            >  2:1177762/1‑140 (MQ=255)
                                                                                                                                     ccTTTTAAAGTTGTATGACACGGTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGc  <  1:124220/140‑1 (MQ=255)
                                                                                                                                     ccTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGc  <  1:278868/140‑1 (MQ=255)
                                                                                                                                     ccTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGc  <  1:226737/140‑1 (MQ=255)
                                                                                                                                     ccTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGc  <  1:1529927/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                          
GAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGC  >  NC_000913/3244836‑3245108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: