Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,540 A→G 7.2% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,5400AG 7.2% 305.3 / 11.8 112noncoding (3/120 nt)rrfF5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base A (45/56);  new base G (6/2);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAG  >  NC_000913/3423404‑3423678
                                                                                                                                        |                                                                                                                                          
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCaa                                                                                                                                         >  1:970471/1‑140 (MQ=17)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCaa                                                                                                                                         >  1:970508/1‑140 (MQ=17)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCaa                                                                                                                                         >  1:1226559/1‑140 (MQ=17)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                                                         >  1:573567/1‑140 (MQ=37)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                                                         >  1:462963/1‑140 (MQ=37)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                                                         >  1:1510275/1‑140 (MQ=37)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa                                                                                                                                         >  2:230326/1‑140 (MQ=17)
gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa                                                                                                                                         >  2:474943/1‑140 (MQ=17)
gAGCCGTTTGTTGTAGGTGTTGGCTCGCAGATCCCTACTCTCGCATGGGGAGAGCCCCCACGACCATCGGCGCTCCGGCGGGTCACGGCTGCGTGCGGCATGGCGTCAGGTGGTGCCACCGCGCGCCTGCCGCCAGACaa                                                                                                                                         >  2:1163214/1‑140 (MQ=2)
 aGCCTTTTGTTTTATTAGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACTCTACCAGCGGCGCTACTGCGTTTCACTGCTGAGTTCGGCATGGGGTCAGGTGGGACCTCCGCGCTAGTGCCGCCCGACaaa                                                                                                                                        >  1:1627804/1‑140 (MQ=11)
   ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAAtt                                                                                                                                      <  2:359467/140‑1 (MQ=37)
      tttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCtg                                                                                                                                   <  2:1442766/140‑2 (MQ=9)
             tATTTGATGTCAGGCAGTTCACTACTCTCGCATGGGGAGACCCCACACTACCAACGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAAGTCTTATATaaa                                                                                                                            >  2:888197/1‑139 (MQ=11)
                  gATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc                                                                                                                       <  1:1281587/140‑1 (MQ=255)
                    tGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa                                                                                                                     >  2:38976/1‑140 (MQ=255)
                    tGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTATAAAATGCCGa                                                                                                                     >  2:1420911/1‑140 (MQ=255)
                    tGTCTGGCAGATCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAGATTCTTTTCTAATATGCCGa                                                                                                                     >  2:1420784/1‑140 (MQ=255)
                        tGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTGTTATATTCTGCCGaagat                                                                                                                 >  2:1353360/1‑137 (MQ=255)
                         ggCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTTCCGAACttt                                                                                                                >  1:927375/1‑140 (MQ=255)
                         ggCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtcc                                                                                                                >  1:1418626/1‑138 (MQ=255)
                            aGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc                                                                                                             <  1:38976/140‑1 (MQ=255)
                            aGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAACAGCCGAACTTTAAc                                                                                                             <  1:604870/140‑1 (MQ=255)
                               tCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTa                                                                                                          <  2:1578822/140‑1 (MQ=255)
                                       ctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg                                                                                                  <  2:1560711/140‑1 (MQ=255)
                                       ctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg                                                                                                  <  1:737859/140‑1 (MQ=255)
                                       ctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg                                                                                                  <  1:884144/140‑1 (MQ=255)
                                       ctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg                                                                                                  <  1:738066/140‑1 (MQ=255)
                                       ctcGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTGTTATAATCTGCCGAACATGAACCAAAAATgtgg                                                                                                  >  2:230411/1‑140 (MQ=14)
                                         cGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtggtg                                                                                                <  1:386492/140‑1 (MQ=255)
                                           cATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGTCAAATTCTTTTATAAACTGCCGAACTTTAACCTAAAAAGTGGTGCt                                                                                              >  2:1311837/1‑140 (MQ=255)
                                                 gagaCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAcc                                                                                        <  1:984213/140‑1 (MQ=255)
                                                   gaCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCa                                                                                      <  2:692332/140‑1 (MQ=255)
                                                    aCCCCACACTACCATCGGCGCTACGGCGTTTCGCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAGCTGCCGAACTTGAACCTAAAAAGTTTTGCTGAAACCCag                                                                                     >  2:175486/1‑140 (MQ=18)
                                                    aCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGTCAAATTCTTTTCTAAACTGCCGAACGTTATACAAAAAAGTGGAGCTGATACCCag                                                                                     >  2:1307077/1‑140 (MQ=17)
                                                    aCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACACATTCTTTTCTAATCTGCCGAACTTTAACATAAAAAGTGGTGCTGTGACCCag                                                                                     >  2:418378/1‑140 (MQ=255)
                                                    aCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACATAAAAAGTGGTGCTGATACCCag                                                                                     >  1:116093/1‑140 (MQ=255)
                                                    aCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTATAAACTGCCGAACGTTAACCGAAAATGTGGAGGTGAAACCCag                                                                                     >  1:115887/1‑140 (MQ=17)
                                                    aCCCCACACTACCATCGGCGCTACGGCGTTTCACGTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAGCTGCCGAACTTTAACATAAAAATTGGTGCTGATAccctg                                                                                     >  1:1355231/1‑138 (MQ=18)
                                                         acacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGTCAAATTCTTTTCTATTCTGCCGATCGTGAACCTAAAAAGTGGTGCTGATACCCAGAGTCg                                                                                >  2:1282992/1‑140 (MQ=18)
                                                         acacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTTTTCTAAACTGCCGAACTTTAACCAAAAAAGTGGTGCTGAGACCCAGAGtag                                                                                >  2:1552138/1‑138 (MQ=18)
                                                         acacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATATGCCGAACTTTAACCTAAAAAGAGGTGCTGATACCAAGAGGCg                                                                                >  2:876775/1‑140 (MQ=255)
                                                          cacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCCGAGTCGa                                                                               >  1:351141/1‑140 (MQ=255)
                                                           acTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGaa                                                                              <  1:927635/140‑1 (MQ=255)
                                                               ccctCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTAAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTgg                                                                          <  1:1231731/137‑1 (MQ=255)
                                                               ccATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTgg                                                                          <  2:816458/140‑1 (MQ=255)
                                                               ccATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTgg                                                                          <  2:1324919/140‑1 (MQ=255)
                                                               ccATCGGCGCTACGGCGTTTCACTACTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTgg                                                                          <  1:1617540/140‑1 (MQ=255)
                                                                  tCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATTGGGTCAGCTTGGACCACCGCGCTACGGCCGCCAGACAAATTCTTTTATAAGCTCCCGAAAGTGACCCTAAATAGTGGTGGTTAAACCCAGAGTCGGAGTTGGGa                                                                       >  2:1126676/1‑140 (MQ=9)
                                                                  tCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTTGGGa                                                                       >  1:609114/1‑140 (MQ=255)
                                                                    ggCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGAcc                                                                     <  2:629977/140‑1 (MQ=255)
                                                                     gcgcTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCt                                                                    <  1:1347788/140‑1 (MQ=255)
                                                                     gcgcAACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCt                                                                    <  1:639777/140‑1 (MQ=255)
                                                                         tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAcc                                                                <  1:226403/140‑1 (MQ=255)
                                                                         tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAcc                                                                <  2:281217/140‑1 (MQ=255)
                                                                         tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAcc                                                                <  2:354379/140‑1 (MQ=255)
                                                                         tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAcc                                                                <  1:37853/140‑1 (MQ=255)
                                                                         tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAACTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAcc                                                                <  1:1230261/140‑1 (MQ=255)
                                                                                tttCTCTTCTGAGTTCGGCATGGGGTCAGGTGGGTCCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCTAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCa                                                         <  2:1149775/140‑1 (MQ=255)
                                                                                   cACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                      >  2:1362920/1‑140 (MQ=255)
                                                                                   cACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                      <  1:1155444/140‑1 (MQ=255)
                                                                                   cACTTCAGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                      >  2:254460/1‑140 (MQ=255)
                                                                                        cTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcg                                                 <  2:1259902/140‑1 (MQ=255)
                                                                                        cTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcg                                                 <  2:1259888/140‑1 (MQ=255)
                                                                                         tGAGTTCGGCTTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcgc                                                <  2:808349/140‑1 (MQ=255)
                                                                                         tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcgc                                                <  2:41764/140‑1 (MQ=255)
                                                                                         tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCAGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcgc                                                <  1:1590504/140‑1 (MQ=255)
                                                                                             ttCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAACTTCTTTTCTAATCTGCCGAACTTTAACCTAAACAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTa                                            <  1:174435/140‑1 (MQ=255)
                                                                                             ttCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTa                                            <  2:573567/140‑1 (MQ=255)
                                                                                             ttCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTa                                            <  1:1138010/140‑1 (MQ=255)
                                                                                             ttCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCACTa                                            <  1:1507321/140‑1 (MQ=255)
                                                                                               cGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAcc                                          >  2:448629/1‑140 (MQ=255)
                                                                                               cGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAcc                                          >  2:1389077/1‑140 (MQ=255)
                                                                                                ggCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCTAACAGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCa                                         >  1:370925/1‑140 (MQ=255)
                                                                                                ggCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCa                                         >  1:247430/1‑140 (MQ=255)
                                                                                                  cagggggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGAGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                       <  1:1125203/137‑1 (MQ=255)
                                                                                                  cATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                       <  2:476611/140‑1 (MQ=255)
                                                                                                      gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACACCTTCTTTTCTACTCTGCCGAACTTTAACCAAAAAAGTGGTGCTGAGACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCACCTGAg                                   <  2:1364050/140‑1 (MQ=37)
                                                                                                      gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATACTTGACTAATCTGCCGAACTTTAACCTCACAAGAGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                   <  2:755633/140‑1 (MQ=37)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTATCCTATAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCaaat                             >  2:438745/1‑140 (MQ=255)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                             >  1:106856/1‑140 (MQ=255)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                             >  1:631555/1‑140 (MQ=255)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                             >  2:605296/1‑140 (MQ=255)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                             >  1:442354/1‑140 (MQ=255)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTATAATCTGCCGAACTTAAACCTAAAAATTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTAACCAAGGGTTCGCACAACCAACTGAGCCataa                             >  2:748067/1‑139 (MQ=38)
                                                                                                            ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCGGTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGACGAACTGGGGACCTCACCCTTACCAAGGGTGCGATCTACCTTATGAGCCatat                             >  1:72155/1‑140 (MQ=38)
                                                                                                                ggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGTGCCATATCAGc                         >  1:590902/1‑140 (MQ=255)
                                                                                                                       cgcgCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaa                  >  1:436452/1‑140 (MQ=255)
                                                                                                                       cgcgCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaa                  >  1:204518/1‑140 (MQ=255)
                                                                                                                       cgcgCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGAGCTCTACCAACTGAGCCATATCAGCACGCTaa                  >  1:44837/1‑140 (MQ=255)
                                                                                                                           cTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAttt              >  2:178346/1‑140 (MQ=255)
                                                                                                                            taatGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg             <  2:1357729/137‑1 (MQ=255)
                                                                                                                            tACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg             <  1:639540/140‑1 (MQ=255)
                                                                                                                                    ccAGACATATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGTTTCCCAGAGTCGTTCTGGGGTCCTCTCCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg     <  2:657815/140‑1 (MQ=37)
                                                                                                                                    ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg     <  2:462963/140‑1 (MQ=255)
                                                                                                                                    ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg     <  1:248803/140‑1 (MQ=255)
                                                                                                                                    ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg     <  1:248802/140‑1 (MQ=255)
                                                                                                                                    ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg     <  1:248788/140‑1 (MQ=255)
                                                                                                                                    ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg     <  2:849770/140‑1 (MQ=255)
                                                                                                                                      aGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCa   <  2:195430/140‑1 (MQ=255)
                                                                                                                                       gACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg  <  2:724390/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAG  >  NC_000913/3423404‑3423678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: