Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,478,768 C→T 5.9% A9T (GCG→ACG)  yheV ← DUF2387 family putative metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,478,7680CT 5.9% 233.6 / 4.0 68A9T (GCG→ACG) yheVDUF2387 family putative metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (36/28);  new base T (2/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

TTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGT  >  NC_000913/3478629‑3478907
                                                                                                                                            |                                                                                                                                           
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:1588234/1‑140 (MQ=255)
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:901670/1‑140 (MQ=255)
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:684856/1‑140 (MQ=255)
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:684823/1‑140 (MQ=255)
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:567373/1‑140 (MQ=255)
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCAGTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:977919/1‑140 (MQ=255)
ttGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACAATCAACAATATCAATATTATTTTCGCGCCACAACGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGc                                                                                                                                             >  1:1588239/1‑140 (MQ=255)
      aCGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATaaa                                                                                                                                       <  2:1070951/140‑1 (MQ=255)
            tCGGGGGCCTCTTTTTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTTCGCCTGAAAGGCCGGGCAATTTTGCGCCCGCAATAAAACGtt                                                                                                                                  <  1:1114013/140‑1 (MQ=255)
                  gCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACAAACAACAAGATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGAt                                                                                                                           >  2:711770/1‑140 (MQ=255)
                     tcttTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGc                                                                                                                        <  1:1580759/140‑1 (MQ=255)
                          gTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCt                                                                                                                   <  2:695832/140‑1 (MQ=255)
                            cTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCttt                                                                                                                 >  2:507070/1‑140 (MQ=255)
                            cTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCttt                                                                                                                 <  2:1103146/140‑1 (MQ=255)
                            cTGCGTCACGCATCTGATGTCCGCCCTTAACACAGTCAACAATATCAATCTTATTTTCGCGCCACATCGCCATGGAATCCTGCGCCTGTCAGGCCGGGCATTTTGCGCCCGCAATAAACCGTTTTCGGATTGCCATCttt                                                                                                                 <  2:1605752/140‑1 (MQ=255)
                               cTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTAcc                                                                                                              <  1:1075534/140‑1 (MQ=255)
                                 tctcGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTTTTTTCGCGCCACATCGCAATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCct                                                                                                            <  1:341265/140‑1 (MQ=255)
                                 tctcGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCct                                                                                                            <  2:280944/140‑1 (MQ=255)
                                      cATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                       <  2:929075/140‑1 (MQ=255)
                                      cATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                       <  1:1617601/140‑1 (MQ=255)
                                      cATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                       <  2:248087/140‑1 (MQ=255)
                                         cTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGATTTCGGATAGCCATCTTTACCCTCGACTCaa                                                                                                    >  1:1668737/1‑140 (MQ=255)
                                         cTGATGTCAGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATAATGCGCCTGACAGGCAGTGAATTTTGCGCCCGCAATAAAACTTTTTCGGATTGCCATATTTACCCACTACTCaa                                                                                                    >  1:1415972/1‑140 (MQ=255)
                                          tGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCaaa                                                                                                   <  1:507070/140‑1 (MQ=255)
                                                 cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                            >  2:962079/1‑140 (MQ=255)
                                                 cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                            >  2:1622703/1‑140 (MQ=255)
                                                 cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                            >  1:858523/1‑140 (MQ=255)
                                                 cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                            >  2:833725/1‑140 (MQ=255)
                                                  gCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATccc                                                                                           <  1:1667114/140‑1 (MQ=255)
                                                   cACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCAATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCa                                                                                          >  1:402155/1‑140 (MQ=255)
                                                          cacaTTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCGGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATTAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTcc                                                                                   <  2:723735/140‑1 (MQ=255)
                                                              ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                               >  2:629533/1‑140 (MQ=255)
                                                                caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                             >  2:221172/1‑140 (MQ=255)
                                                                 aacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGTAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                            >  2:1636657/1‑140 (MQ=255)
                                                                 aacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGTAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                            >  2:1636522/1‑140 (MQ=255)
                                                                  acaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGcg                                                                           >  2:1510077/1‑140 (MQ=255)
                                                                   caatatcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGcgt                                                                          <  2:677292/140‑1 (MQ=255)
                                                                       atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTTGCGTCGtt                                                                      >  2:592440/1‑140 (MQ=255)
                                                                       atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTTGCGTCGtt                                                                      >  2:592601/1‑140 (MQ=255)
                                                                           atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                  >  2:1027301/1‑140 (MQ=255)
                                                                           atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                  >  2:1410091/1‑140 (MQ=255)
                                                                           atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATAGCCATCATTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                  >  2:697158/1‑140 (MQ=255)
                                                                                 ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCAt                                                            >  2:185432/1‑140 (MQ=255)
                                                                                 ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTGCTTGTTGCAt                                                            >  2:185639/1‑140 (MQ=255)
                                                                                    tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTc                                                         >  2:4047/1‑140 (MQ=255)
                                                                                    tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAAGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTc                                                         >  2:4214/1‑140 (MQ=255)
                                                                                      gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAATCGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTGCTTGTTGCATTTCAc                                                       >  2:311076/1‑140 (MQ=255)
                                                                                      gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                       >  2:310912/1‑140 (MQ=255)
                                                                                                gCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGTAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                             <  1:1636657/140‑1 (MQ=255)
                                                                                                gCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGTAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                             <  1:1636522/140‑1 (MQ=255)
                                                                                                      gAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTc                                       >  2:559591/1‑140 (MQ=255)
                                                                                                       aaTCCAGCGCCTGACAGGCCGGGCATTTAGCGCCCGCAATAAAACGTTATCGGAATGCCAACTTAACCCTCTACTCAAAATCAACCCAGCCGACCAGCTGGCGTCGTTCTTGTTGCATTTCACCCTGGAAAATCTCTTcc                                      >  2:732665/1‑140 (MQ=255)
                                                                                                         tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAg                                    >  2:733921/1‑140 (MQ=255)
                                                                                                            tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                 >  2:1151569/1‑140 (MQ=255)
                                                                                                            tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                 >  2:494972/1‑140 (MQ=255)
                                                                                                                  tGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                           <  2:20872/140‑1 (MQ=255)
                                                                                                                    aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                         >  1:995911/1‑140 (MQ=255)
                                                                                                                    aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                         >  1:522560/1‑140 (MQ=255)
                                                                                                                    aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                         <  2:815930/140‑1 (MQ=255)
                                                                                                                        gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTc                     >  1:287449/1‑140 (MQ=255)
                                                                                                                        gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTc                     >  2:1180527/1‑140 (MQ=255)
                                                                                                                        gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGAGTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTc                     >  2:586292/1‑140 (MQ=255)
                                                                                                                          cGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTccc                   >  1:1037871/1‑140 (MQ=255)
                                                                                                                           gggCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATACATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCAAGTTCGCGTCGGGCTTCCCt                  >  2:1374274/1‑140 (MQ=255)
                                                                                                                                   ttGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga           <  2:949207/140‑1 (MQ=255)
                                                                                                                                   ttGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga           >  2:431352/1‑140 (MQ=255)
                                                                                                                                   ttGCGCCCGCAATAAAACGTTGTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga           <  2:949761/140‑1 (MQ=255)
                                                                                                                                        cccGCAATCAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCATATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt      <  2:219519/140‑1 (MQ=255)
                                                                                                                                        cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt      <  1:1106914/140‑1 (MQ=255)
                                                                                                                                        cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt      <  2:1111383/140‑1 (MQ=255)
                                                                                                                                         ccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAACTTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAtt     <  1:584079/140‑1 (MQ=255)
                                                                                                                                         ccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAtt     <  2:446639/140‑1 (MQ=255)
                                                                                                                                         ccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAtt     <  2:443497/140‑1 (MQ=255)
                                                                                                                                         ccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAtt     <  1:1383593/140‑1 (MQ=255)
                                                                                                                                           gCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCAACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGGTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTg   <  1:36284/140‑1 (MQ=255)
                                                                                                                                            cAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGCCGGGCTTCCCTGGCGCGAGAAATTTGt  <  1:133105/140‑1 (MQ=255)
                                                                                                                                            cAATAAAACGTTTTCGGAGTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGt  <  2:818723/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGT  >  NC_000913/3478629‑3478907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: