Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,511,719 C→A 5.2% R209L (CGA→CTA)  yhfZ ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,511,7190CA 5.2% 286.7 / 2.0 85R209L (CGA→CTA) yhfZputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (38/42);  new base A (2/3);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGC  >  NC_000913/3511584‑3511856
                                                                                                                                       |                                                                                                                                          
cgcgGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTc                                                                                                                                        <  1:1070433/140‑1 (MQ=255)
cgcgGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTc                                                                                                                                        <  1:59762/140‑1 (MQ=255)
cgcgGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTc                                                                                                                                        <  1:1572257/140‑1 (MQ=255)
  cgGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCTGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCca                                                                                                                                      <  1:904905/140‑1 (MQ=255)
                 cggcgTCGCCGCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACCTCGCCTTTCACAATGCGGTGTACACACACGTCAAAAGAGAGATCGACGCGGGCCACATCACAACCGCCac                                                                                                                      >  2:1632489/1‑139 (MQ=255)
                    cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                   >  1:827926/1‑140 (MQ=255)
                    cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTAAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                   >  1:1407476/1‑140 (MQ=255)
                     gTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaaa                                                                                                                  >  2:1342126/1‑140 (MQ=255)
                                  ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACACTGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATg                                                                                                     <  2:1307674/140‑1 (MQ=255)
                                  ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTAGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATg                                                                                                     <  1:1453386/140‑1 (MQ=255)
                                  ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTAGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATg                                                                                                     <  1:1576661/140‑1 (MQ=255)
                                   cAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATg                                                                                                     <  2:1215478/140‑1 (MQ=255)
                                    aGCATGTTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGAt                                                                                                   >  2:740803/1‑140 (MQ=255)
                                    aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCTGTCATTAt                                                                                                   >  1:94883/1‑140 (MQ=255)
                                    aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGAt                                                                                                   >  2:1521126/1‑140 (MQ=255)
                                    aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGATGTAAACTCTCGTGATAAGAGAGATCGACACGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGAt                                                                                                   >  1:838756/1‑140 (MQ=255)
                                       aTGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGTTCATGAtttt                                                                                                >  2:629799/1‑140 (MQ=255)
                                          gTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAACATCGGTCATGATTTTCTGa                                                                                            >  1:33123/1‑140 (MQ=255)
                                           tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGa                                                                                            >  2:366083/1‑140 (MQ=255)
                                            ctgTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGAt                                                                                           <  1:1213247/138‑1 (MQ=255)
                                            cAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGAt                                                                                           <  2:1588880/140‑1 (MQ=255)
                                              gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATcc                                                                                         >  1:16452/1‑140 (MQ=255)
                                                  gTTTACCGCCACCACGACCCAGATCACCGCATCGACACCGCCTTAAACAATGCGTAGAAACCTCTCCTTAAAACAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                     <  2:1642302/140‑1 (MQ=255)
                                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTCACACTGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                   <  2:533510/140‑1 (MQ=255)
                                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                   >  1:565235/1‑140 (MQ=255)
                                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGAGTTTCTGATCCGCCGaa                                                                                   >  1:583068/1‑140 (MQ=255)
                                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCATTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                   <  1:550199/140‑1 (MQ=255)
                                                       ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAATATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                <  2:1101754/140‑1 (MQ=255)
                                                       ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                <  2:23082/140‑1 (MQ=255)
                                                       ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                <  2:22888/140‑1 (MQ=255)
                                                       ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACAGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                <  2:1599224/140‑1 (MQ=255)
                                                         gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGtt                                                                              >  1:979733/1‑140 (MQ=255)
                                                            accacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc                                                                           <  1:893330/140‑1 (MQ=255)
                                                             ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATcc                                                                          >  2:1414118/1‑140 (MQ=255)
                                                                    ccAGATCACCGCATCGACATCGCCTTTAACTATGCGTTGTAATCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                   <  2:1207500/140‑1 (MQ=255)
                                                                    ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGCGAGATCGACTCGTTCCACATCACTACCGCCAAAAACAACATCGGTCTTGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                   <  2:1321539/140‑1 (MQ=255)
                                                                    ccAGATCACCGCATCGACATCGCCTTTAACAAAGCGTTGTAAACTCTCGTGATAAGAGAGATCTACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                   <  2:1628602/140‑1 (MQ=255)
                                                                      aGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                 <  1:874449/140‑1 (MQ=255)
                                                                           aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                            <  1:116182/140‑1 (MQ=255)
                                                                           aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCACCCCCACGCGCta                                                            >  1:201486/1‑139 (MQ=255)
                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                          >  1:829947/1‑140 (MQ=255)
                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                          >  1:589132/1‑140 (MQ=255)
                                                                                    aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGc                                                   >  1:41230/1‑140 (MQ=255)
                                                                                         gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                              >  2:139307/1‑140 (MQ=255)
                                                                                              tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCt                                         >  1:696093/1‑140 (MQ=255)
                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  1:811774/1‑140 (MQ=255)
                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  1:1210369/1‑140 (MQ=255)
                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  2:114472/1‑140 (MQ=255)
                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  2:730573/1‑140 (MQ=255)
                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  1:1574274/1‑140 (MQ=255)
                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  2:945178/1‑140 (MQ=255)
                                                                                                    agCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                   >  1:1681360/2‑140 (MQ=255)
                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                  <  2:599272/140‑1 (MQ=255)
                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                  <  1:290180/140‑1 (MQ=255)
                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                  >  1:389034/1‑140 (MQ=255)
                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                  <  2:930957/140‑1 (MQ=255)
                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGATTGCGGACTCGCCTTTACGGc                                  <  2:597196/140‑1 (MQ=255)
                                                                                                             aaCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                          <  2:325410/140‑1 (MQ=255)
                                                                                                                tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                       >  1:922500/1‑140 (MQ=255)
                                                                                                                  tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                     <  2:491137/140‑1 (MQ=255)
                                                                                                                  tagTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                     <  1:1658106/138‑1 (MQ=255)
                                                                                                                   cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                    >  1:389204/1‑140 (MQ=255)
                                                                                                                   cGTGATAAGAGAGATCGACTAGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                    >  2:909513/1‑140 (MQ=255)
                                                                                                                   cGTGATAAGAGAGATCGACTAGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                    >  2:909418/1‑140 (MQ=255)
                                                                                                                    gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCt                   <  1:1317256/140‑1 (MQ=255)
                                                                                                                    gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCt                   >  2:1200902/1‑140 (MQ=255)
                                                                                                                    gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCt                   <  1:1317216/140‑1 (MQ=255)
                                                                                                                     tGATAAGAGAGATCGACTCGGTCCACATCACTACCGCCAAAAAAAACCTCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                  <  1:1683299/140‑1 (MQ=255)
                                                                                                                       caaaGAGAGCTCGACTCGTTCCACACCACTACCGCCAAAACAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTATGCGGACTCGCCTTTACGTCAAATCAACTGGTGCTCGc                <  2:1607859/138‑1 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                <  2:647285/140‑1 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                <  2:98248/140‑1 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                <  2:857417/140‑1 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                <  1:637502/140‑1 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                >  1:625394/1‑140 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                <  1:532045/140‑1 (MQ=255)
                                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                <  1:324472/140‑1 (MQ=255)
                                                                                                                         cagagagATCGTCTAGTTCCACATCACGACCCCCAAAAAAAACAGCGGTCATGATCTTCTGATCCGCCGACCCGGATTCCAGCCCCACGCGCGTCACGTTTGCTGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCa              <  2:890498/139‑1 (MQ=255)
                                                                                                                          agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa             >  2:1313047/1‑140 (MQ=255)
                                                                                                                            agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg           <  2:322615/140‑1 (MQ=255)
                                                                                                                               gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAg        <  2:760979/140‑1 (MQ=255)
                                                                                                                                aTCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg       >  2:1142989/1‑140 (MQ=255)
                                                                                                                                    aCTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg   <  2:423113/140‑1 (MQ=255)
                                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTgcgc  >  2:1660748/1‑140 (MQ=255)
                                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc  <  2:696093/140‑1 (MQ=255)
                                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc  >  2:29616/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGC  >  NC_000913/3511584‑3511856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: