Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,546,954 G→T 8.1% A132A (GCG→GCT gntT → gluconate transporter, high‑affinity GNT I system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,546,9540GT 8.1% 288.0 / 6.8 86A132A (GCG→GCTgntTgluconate transporter, high‑affinity GNT I system
Reads supporting (aligned to +/- strand):  ref base G (39/40);  new base T (4/3);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTACGGTA  >  NC_000913/3546815‑3547093
                                                                                                                                           |                                                                                                                                           
ccacGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGACGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCg                                                                                                                                             >  2:442/1‑140 (MQ=255)
 cacGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGa                                                                                                                                            <  1:1491834/140‑1 (MQ=255)
  acGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGaa                                                                                                                                           <  1:246045/140‑1 (MQ=255)
  acGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTATTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTATACCATCGCGGCTTCTGCGaa                                                                                                                                           <  1:1185705/140‑1 (MQ=255)
        aTTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTGTACCATCGCGGCTTCTGCGAATATTcc                                                                                                                                     >  2:576962/1‑140 (MQ=255)
         ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTTCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                                                                    <  1:516205/140‑1 (MQ=255)
         ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                                                                    <  1:1316883/140‑1 (MQ=255)
         ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                                                                    <  1:322009/140‑1 (MQ=255)
          tGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCAc                                                                                                                                   >  2:963260/1‑140 (MQ=255)
            ccAAATTTGGTAAACAACACCTCCAGTGGGCGGTGGTACTGACCGGTTTTTCCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCActg                                                                                                                                 <  2:888115/140‑1 (MQ=255)
            ccAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCActg                                                                                                                                 <  2:301873/140‑1 (MQ=255)
                  ttGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTAt                                                                                                                           >  1:735179/1‑140 (MQ=255)
                     gTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGtt                                                                                                                        <  2:1654299/140‑1 (MQ=255)
                                aTCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAAt                                                                                                             <  2:1255006/140‑1 (MQ=255)
                                     gTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg                                                                                                        <  1:799679/140‑1 (MQ=255)
                                     gTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGAATGTTGGTGTACCAATggcgg                                                                                                        <  1:801482/140‑1 (MQ=255)
                                        ggCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTg                                                                                                     >  1:1299327/1‑140 (MQ=255)
                                        ggCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTg                                                                                                     >  1:1299107/1‑140 (MQ=255)
                                        ggCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTg                                                                                                     >  1:1299125/1‑140 (MQ=255)
                                          cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                                                   <  2:1620289/140‑1 (MQ=255)
                                          cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                                                   <  2:1100788/140‑1 (MQ=255)
                                          cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                                                   <  1:221533/140‑1 (MQ=255)
                                          cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                                                   <  2:350750/140‑1 (MQ=255)
                                          cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                                                   <  2:793077/140‑1 (MQ=255)
                                          cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                                                   <  1:1311145/140‑1 (MQ=255)
                                           ggtggtACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCAc                                                                                                  <  2:1031406/140‑1 (MQ=255)
                                                 aCTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtctg                                                                                            <  1:22176/140‑1 (MQ=255)
                                                  cTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtctgt                                                                                           >  1:1385790/1‑140 (MQ=255)
                                                  cTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTAACATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtctgt                                                                                           >  1:1030291/1‑140 (MQ=255)
                                                     aCCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGGGTACCATTGGCGGCTGCACTGTCTGTGAc                                                                                        >  2:1337501/1‑140 (MQ=255)
                                                     aCCGGTTTTACCGTTGGTGTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCGGTCTGTTGGTGTACCAATGGCGGCTGCACTGTCGCTGAc                                                                                        >  2:613045/1‑140 (MQ=255)
                                                       cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGAccc                                                                                      >  1:260865/1‑140 (MQ=255)
                                                         gTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGTGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATCTTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCAc                                                                                    >  2:174073/1‑140 (MQ=255)
                                                         gTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCAc                                                                                    >  2:32478/1‑140 (MQ=255)
                                                                    ggTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGcc                                                                         >  2:748336/1‑140 (MQ=255)
                                                                                ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                                                             >  1:136700/1‑140 (MQ=255)
                                                                                ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                                                             >  1:1271015/1‑140 (MQ=255)
                                                                                ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                                                             >  2:27216/1‑140 (MQ=255)
                                                                                ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                                                             >  1:426300/1‑140 (MQ=255)
                                                                                ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                                                             >  2:1489993/1‑140 (MQ=255)
                                                                                 tCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGt                                                            <  2:832417/140‑1 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:252068/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGTCGGCTGCAATGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:35421/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCCCCGCATCCGGGTCCGACTGCg                                                   >  2:1367462/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  2:563419/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  2:521247/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:1379976/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  2:697734/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:1661290/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:411887/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:35396/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:898859/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTCTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  2:307172/1‑140 (MQ=255)
                                                                                          tGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGATTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                                                   >  1:57305/1‑140 (MQ=255)
                                                                                            ggCTTTTTGATGATGCTGCCGCAGGTCTTAACCATCGCGAAATCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAAAGCCGGCTGCACAGACTGAGACCCACGGCTTCCTCCCAACGCATCCGGGTCCCACTGCGag                                                 >  2:22284/1‑139 (MQ=255)
                                                                                              cTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTg                                               <  1:18093/140‑1 (MQ=255)
                                                                                              cTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTg                                               <  1:15085/140‑1 (MQ=255)
                                                                                                        aTGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAtttt                                     >  2:1232028/1‑140 (MQ=255)
                                                                                                        aTGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAtttt                                     >  1:851299/1‑140 (MQ=255)
                                                                                                        aTGCTGCCGCTGGAGTTTACAATCGCGGCATCAGCGAATATTCCACTGCTGTAGGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCATCCTGACACCGCATCCGGGTCCGACAGCGATTGCCACCAttta                                     >  1:849373/1‑139 (MQ=255)
                                                                                                             gcCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATg                                >  1:818041/1‑140 (MQ=255)
                                                                                                             gcCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATg                                >  1:767337/1‑140 (MQ=255)
                                                                                                                  tGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGat                           <  2:410272/140‑1 (MQ=255)
                                                                                                                  tGGTGTTTACCATCGCGGATTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGat                           <  2:299966/140‑1 (MQ=255)
                                                                                                                   ggTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCAGGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGata                          <  2:543658/140‑1 (MQ=255)
                                                                                                                      gtTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATgg                       >  2:1321070/1‑140 (MQ=255)
                                                                                                                         tACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTa                    <  2:1299327/140‑1 (MQ=255)
                                                                                                                         tACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTa                    <  2:1299107/140‑1 (MQ=255)
                                                                                                                         tACCATCGCGGCTTCTGCTAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTa                    <  2:1299125/140‑1 (MQ=255)
                                                                                                                         tACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTAAATGCCGATATGGGTa                    <  2:873459/140‑1 (MQ=255)
                                                                                                                         tACCATCGCGGCTTCAGCGAATATACCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGACGATATgggaa                    >  2:679965/1‑138 (MQ=255)
                                                                                                                            cATCACGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTaaaa                 <  2:290429/140‑1 (MQ=255)
                                                                                                                              tCGCGGCTTCTGCGAATATTCCACTGCGGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAGAAcc               >  2:342846/1‑140 (MQ=255)
                                                                                                                               cgcgGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAAccc              >  2:1584053/1‑140 (MQ=255)
                                                                                                                               cgcgGCTTCAGCGAATATTCCACTGATGGATTTTTGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGTCTTCCTGCCACCGCATCAGGGTCCGACTGAGATTGCAACCATTGTCAATGCCTATATGTGTAAAAccc              >  2:975343/1‑140 (MQ=255)
                                                                                                                                 cgGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCctg            <  1:27216/140‑1 (MQ=255)
                                                                                                                                    cTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCctgctg         <  1:822518/140‑1 (MQ=255)
                                                                                                                                    cTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCctgctg         <  1:307172/140‑1 (MQ=255)
                                                                                                                                     ttCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGt        <  2:991391/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  1:1489993/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  2:260865/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  2:184318/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  1:1038248/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  2:898859/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  1:1021514/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  1:563419/140‑1 (MQ=255)
                                                                                                                                      tCTGCGAATAGTCCACTGCTGAATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTGTCAATGCCGATATGGGTAAAACCCTGCTGTa       <  2:836713/140‑1 (MQ=255)
                                                                                                                                           gAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTACGGTa  <  1:739510/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTACGGTA  >  NC_000913/3546815‑3547093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: